Kansas City Quality Control

Location:

United States

Posted:

August 06, 2014

Contact this candidate

Resume:

CURRICULUM VITAE

ANKIT SHAH

*** * **** **, *** # 307, Contact: 732-***-****

Kansas City, MO-64111

*****.****@****.***

QUALIFICATIONS SUMMARY

Experience in performing in vitro experiments on astrocytes, primary human astrocytes, neuronal and

neuroblastoma (SH-SY5Y) cell-line, PBMC cells, T-cells, CEM cells, U937 monocytes and THP-1 monocytes.

Experience in conducting experiments in non-human primate (Rhesus Macaque) based model for HIV.

Experience in various immunological and molecular biology techniques (Western Blotting, ELISA,

multiplex cytokine assay, immunocytochemistry, immunohistochemistry, confocal microscopy, realtime RT-

PCR), siRNA and primer designing, DNA damage assays, apoptosis studies

Knowledge of basic principles and process of drug development.

Experienced and well-trained in independent experimental designing and conducting hypothesis-driven

research in the field of infectious disease, neurological diseases and basic neuroscience.

Experience in setting up methods and designing experiments using flow cytometer and electroporation.

Experience in PBMC preparation and T-lymphocyte separation from Human and Rhesus Macaque blood

samples.

Experience in plasmid isolation, preparation and purification techniques.

Experience in carrying out in vitro enzymatic assays for various CYPs and determine rate, K, t1/2 and

Cmax.

Experience in performing in vivo experiments (mice):

in vivo oral and intraperitoneal studies (mice) and data analysis using MS Excel, SPSS

Behavioral studies on mice to check the effect of CNS depressants, neurodegenerative disorders.

Maintaining and genotyping a transgenic colony of over 200 mice and performing behavioral and

histological tests to study the neurotoxicity.

Experience in writing experimental protocols, animal protocols, literature reviews, grant reports,

publications, NIH grant proposals. Troubleshoot analytical methods and laboratory instruments

Statistical packages and experience: SPSS, ChemDraw, GraphPad Prism and MS office.

Good communication, computer documentation and presentation skills

RESEARCH PROJECTS

(Awarded the Outstanding Dissertation of the year 2013 award by UMKC)

Dissertation projects: HIV-1 gp120 and methamphetamine-mediated induction of

proinflammatory cytokines/chemokines and oxidative stress in astrocytes: Implications in

neuroinflammation.

To study the effect of MA and gp120 on cytotoxicity and underlying mechanisms in astrocytic model.

To study the effect of gp120 on production of pro-inflammatory cytokines/chemokines

and molecular mechanisms underlying the same using recombinant gp120 protein and

expression vector. (Ph.D.).

To study the effect of methamphetamine on production of pro-inflammatory

II.

cytokines/chemokines and molecular mechanisms underlying the same. (Ph.D.).

To investigate if MA and gp120 interact with each other to produce synergistic or

III.

additive effect on the production of proinflammatory cytokines in astrocytes and investigate the

underlying mechanisms. (Ph.D.)

To investigate the effect of MA and gp120 on oxidative stress and investigate the role of

IV.

various cytochrome P450. (Ph.D.).

To study the role of ER stress in the cytotoxicity induced by gp120 and MA in astrocytes

and neuronal cells and understand various molecular mechanism(s) underlying the same.

Comprehensive proposal: Epigenetic regulation of SOCS in the pathology of schizophrenia.

Other research projects:

Isolation and characterization of PBMC from whole blood and tissue samples such as lymph

node and rectal biopsy from non-human primate (Rhesus Macaque) and estimate the CD3/4/8

profile in the disease progression due to SIV infection.

Preparation of non-human primate brain samples for histological studies in various regions of

the brain.

Preparation and characterization of primary human astrocyte culture from brain.

Enzyme binding studies on interaction of methamphetamine with various cytochrome P450

enzymes.

Breeding and genotyping HIV-1 gp120 transgenic mice.

Studying the histopathology of brain in gp120-tg mice.

RESEARCH ACCOMPLISHMENTS

Demonstrated the inflammatory potential of methamphetamine and HIV-1 gp120.

Demonstrated the molecular mechanism underlying MA and gp120-mediated cytokine

expressions.

Demonstrated the interaction between MA and gp120 to induce pro-inflammatory cytokines and

chemokines and established the role of CYP enzymes in neuroAIDS for the first time.

Studied the biochemical pathways to understand how generation of oxidative stress in astrocytes

due to MA and gp120 play a role in cytotoxicity.

Studied the effect of MA on LPS-induced monocytes to produce pro-inflammatory

cytokines/chemokines.

Studied the role of ER-stress in MA and gp120-mediated cytotoxicity in astrocytes (Most recent

project).

PROFESSIONAL EXPERIENCE

Post-Doctoral Associate Jan 2014 – Present

Graduate Teaching Assistant

PharmD (Pharmacotherapy I & III) Fall2008

PharmD (Pharmacotherapy II) Spring 2009

PharmD (Pharmacotherapy I & III, Pharmacoeconomics) Fall2009

Pharm D & Graduate (Pharmacology-II) Spring 2010

Graduate (Pharmacology-I) Fall 2010

Graduate Research Assistant

Dr. Anil Kumar’s lab Jan-2011 - present

The lab chiefly concentrates on the following:

• Effect of drug and alcohol abuse on pathogenesis of AIDS.

• Identification of target of immune responses in HIV-1 during natural infection

• Compartmentalization of HIV during natural infection

• Vaccine approaches in monkey model of AIDS

Teaching experience

I have been actively involved in training new students with the existing techniques in the lab. I have

acted as a preceptor for multiple students during rotation and internships in the lab.

PROFESSIONAL AFFILIATIONS

Member of Society on Neuroimmune Pharmacology (SNIP).

Member of Science Advisory Board

Reviewer for PLoS One

SELECTED PUBLICATIONS

Research articles (First author)

Shah, A., et al. “HIV-1 gp120 induces unfolded protein response (UPR) via endoplasmic reticulum

(ER)-stress genes: Role in astrocyte toxicity” In preparation.

Shah, A. et al. “HIVgp120 and methamphetamine-mediated oxidative stress induces astrocyte

apoptosis via cytochrome P450 2E1” Cell death and Disease (2013) 4, e850

Shah, A., et al. “Synergistic cooperation between methamphetamine and HIV-1 gp120 through the

PI3K/Akt pathway induces IL-6 but notILl-8 expression in astrocytes.” PLoS One.

2012;7(12):e52060

Shah, A., et al. Involvement of Metabotropic Glutamate Receptor-5, AKT/PI3K Signaling and NF-

kB Pathway in Methamphetamine-mediated increase in IL-6 and IL-8 Expression in Astrocytes. J

Neuroinflammation 9 (2012) 52.

Shah, A., et al., HIV-1 envelope protein gp120 up regulates CCL5 production in astrocytes which

can be circumvented by gp120-specific siRNA and inhibitors of NF- kappa B pathway BBRC, 2011

Oct 14;414(1):112-7.

Shah, A., et al., HIV-1 gp120 Induces Expression of IL-6 through a Nuclear Factor-Kappa B-

Dependent Mechanism: Suppression by gp120 Specific Small Interfering RNA. PLoS One, 2011.

6(6): p. e21261.

Shah, A. and A. Kumar., HIV-1 gp120-mediated increases in IL-8 production in astrocytes are

mediated through the NF-kappaB pathway and can be silenced by gp120-specific siRNA. J

Neuroinflammation, 2010. 7: p. 96.

Shah, A., Shah, J.S., Sen, D.J., Comparison of pharmacological screening of CNS depressant

action of fused ring heterocyclic adduct with fused ring aromatic with benzodiazepine as standard .

Journal of International Drug Development and Research.2010: Vol.2 (1).

Research articles (Co-author)

Jackson, A., Shah, A., et al. Methamphetamine alters cell cycle regulation in astrocytes. In

Preparation

2. Earla, R., Shah, A., et al. Enhanced methamphetamine metabolism in rhesus macaque compared

to human by cytochrome P450 pathways: An analysis using a novel LCMS/MS method and

docking study. Drug Metabolism and Disposition 2014 (submitted).

3. Xun, L., Shah, A. et al. HIV-1Nef Induces CCL5 production in astrocytes through p38 MAPK and

PI3K/Akt pathway and utilizes NF-κB, CEBP and AP-1 transcription factors.” Sci Rep 4: 4450

Nookala, A., Shah, A. et al. HIV-1 Tat-mediated induction of CCL5 in Astrocytes involves NFκB,

AP1, C/EBPα and C/EBPγ transcription factors and JAK, PI3K/Akt and p38MAPK signaling

pathways.” PLoS One, 8(11), e78855.

5. Gangwani, M., Shah, A. et al. “Human Immunodeficiency Virus Type 1 Type 1 Viral Protein R (Vpr)

Induces CCL5 Expression in Astrocytes via PI-3K and MAPK Signaling Pathways.” Journal of

Neuroinflammation 2013, 10:136.

6. Liu, X., Shah, A., et al. “Methamphetamine increases LPS-mediated expression of IL-8, TNF-α

and IL-1β in human macrophages through common signaling pathways”. PLoS One 7 (2012)

e33822.

7. Jin, M., Shah, A., et al., A LC-MS/MS Method for Concurrent Determination of Nicotine Metabolites

and Role of CYP2A6 in Nicotine Metabolism in U937 Macrophages: Implications in Oxidative

Stress in HIV + Smokers. J Neuroimmune Pharmacol. 2012 Mar;7(1):289-99.

Review Articles:

P.S. Silverstein, A. Shah, et al, HIV-1 gp120 and Drugs of Abuse: Interactions in the Central

Nervous System, Curr HIV Res. 2012 Jul;10(5):369-83

2. Silverstein, P., Shah, A., et al., Methamphetamine Toxicity and its Implications During HIV-1

Infection, J Neurovirol. 2011 Oct;17(5):401-15

SELECTED POSTER PRESENTATION

(A) NATIONAL MEETINGS

1. Society on Neuroimmune Pharmacology (SNIP) Annual Meeting:

1. Ankit Shah, Anil Kumar, HIV-1 gp120 induces unfolded protein response (UPR) via endoplasmic

reticulum (ER)-stress genes: Role in astrocyte toxicity at S-NIP conference held at New Orleans,

LA in March, 2014 (Also Invited for Oral symposium)

2. Ankit Shah, Anil Kumar, HIV-1 protein gp120 and methamphetamine co-operate synergistically to

increase oxidative stress in astrocytes: Role of cytochrome P450 . at S-NIP conference held at

San Juan, PR in April, 2013 (Also Invited for Oral symposium)

3. Ankit Shah, Anil Kumar, Signaling mechanisms involved in methamphetamine-mediated

increase in the expressions of IL-6/IL-8 in astrocytes. at S-NIP conference held at Honolulu,

HI in April, 2012.

4. Raeesa Gupte, Ankit Shah, Anil Kumar, Etahnol induced upregulation of IL-6 and IL-8 in

astrocytes is mediated by the NF-kB pathway at S-NIP conference held at Clearwater Beach, FL

in April, 2011.

5. Mengyao Jin, Raeesa Gupte, Ankit Shah, Anil Kumar, Santosh Kumar, The role of cytochrome

P450 on alcohol/nicotine-mediated oxidative stress in U937 macrophages at S-NIP conference

held at Clearwater Beach, FL in April, 2011.

6. Ankit Shah, Anil Kumar, HIV-1 glycoprotein 120 induces the pro-inflammatory cytokine IL-6 via

NF-κB pathway and methamphetamine can synergistically potentiate the gp120-mediated IL-6

induction. at S-NIP conference held at Clearwater Beach, FL in April, 2011. (Also Invited for Oral

symposium)

7. Ankit Shah, Anil Kumar HIV-1 gp120 Induces Expression of IL-6 Through a Nuclear Factor-

Kappa B-dependent Mechanism: Suppression by gp120 Specific Small Interfering RNA in the

poster presentation session for S-NIP conference held at Manhattan beach, CA in April, 2010.

2. Society of Neuroscience (SFN) NIDA Mini convention:

Ankit Shah, Anil Kumar, Methamphetamine increases the expression of pro-inflammatory

cytokines through NF-κB pathway in astrocytes . at NIDA mini-convention on “Frontiers in Addiction

Research” in November, 2011.

(B) REGIONAL MEETINGS

UMKC Health Sciences Research Summit:

Ankit Shah, Anil Kumar, HIV-1 gp120 induces unfolded protein response (UPR) via

endoplasmic reticulum (ER)-stress genes: Role in astrocyte toxicity at Health Sciences

Research Summit 2014 – Kansas City, MO in April, 2014.

Ankit Shah, Anil Kumar, Signaling mechanisms involved in methamphetamine-

mediated increase in the expressions of IL-6/IL-8 in astrocytes.” at Health Sciences

Research Summit 2012 – Kansas City, MO in April, 2012.

Mengyao Jin, Raeesa Gupte, Ankit Shah, Anil Kumar, Santosh Kumar, A LC-MS/MS method

for concurrent determination of CYP2A6-mediated nicotine metabolites in U937 macrophages at

Health Sciences Research Summit 2011 – Kansas City, MO in April, 2011

Ankit Shah, Anil Kumar, HIV-1 glycoprotein 120 induces the pro-inflammatory cytokine

IL-6 via NF-κB pathway and methamphetamine can synergistically potentiate the gp120-

mediated IL-6 induction. at Health Sciences Research Summit 2011 – Kansas City, MO in

April 2011.

Ankit Shah, Anil Kumar, HIV-1 gp120 Induces Expression of IL-6 Through a Nuclear Factor-

Kappa B-dependent Mechanism: Suppression by gp120 Specific Small Interfering RNA. at

Research Day – UMKC, Kansas city, MO in April, 2010.

Ankit Shah, Anil Kumar, HIV-1 gp120 mediated increase in IL-8 production in astrocytes is

mediated through NF-κbeta pathway and can be silenced by gp120 specific siRNA. at Research

Day – UMKC, Kansas city, MO in April, 2010.

HIV-1 gp120 Induces Expression of IL-8 Through a Nuclear Factor-Kappa B-dependent

Mechanism: Suppression by gp120 Specific Small Interfering RNA in the oral presentation session

for the scientific symposium in International conference on Opportunistic Pathogens at AIIMS,

New Delhi, India in September, 2010. (Invited for Oral symposium)

2. Comparison of pharmacological screening of CNS depressant action of fused ring heterocyclic

adduct with fused ring aromatic with benzodiazepine as standard was presented in the poster

session of 10th APTI National Convention, Nagpur, Maharashtra, 17-18 October 2005.

3. Preparation and evaluation of Albumin Microspheres of DiltiazemHCl As a sustained release

drug in the poster session of 9th APTI INTERNATIONAL CONVENTION,Vishakhapattnam,

Andhra Pradesh, 2-3 October 2004

EDUCATION

Interdisciplinary Ph.D. in Pharmacology & Toxicology August 2008-Dec 2013

University of Missouri-Kansas City (UMKC)

GPA: 3.6/4.00

Advisor: Dr. Anil Kumar

MS in Cell & Molecular Biology August 2008 – May 2012

University of Missouri-Kansas City (UMKC)

GPA: 3.5/4.00

Master of Chemistry (Pharmaceutical Concentration) August 2007 – June 2008

(Degree not awarded)

Fairleigh Dickinson University

GPA: 3.7/4.00

Bachelor of Science in Pharmacy 2002 - 2006

Sri Sarvajanik Pharmacy College, Mehsana, India

GPA: 4.0/4.0

ACHIEVEMENTS/AWARDS & HONORS

Scholarships/Fellowships

Awarded with the Outstanding Dissertation of the year 2013 by UMKC

Awarded with Chancellor’s Doctoral Fellowship for 2013-14.

Awarded for best poster at Health Science Research Summit 2012 organized by UMKC.

Awarded with the Dean’s scholarship for 2011 in the Pharmacology & Toxicology Division at

University of Missouri Kansas City.

Awarded with Robert C. Lanman scholarship at UMKC for the academic year 2011-12

Awarded for best poster at Health Science Research Summit 2011 organized by UMKC

Awarded as Best Student of the year 2010 in the Pharmacology & Toxicology Division at

University of Missouri Kansas City.

Travel Grants

Awarded with Early Career Investigator Travel Award to cover the 20th SNIP meeting held at

New Orleans, LA in March 2014

Awarded with SGS Travel Grant to attend SNIP 2013 meeting in San Juan, PR.

Awarded with IDSC Travel Grant to attend SNIP 2013 meeting in San Juan, PR.

Awarded with Early Career Investigator Travel Award to partially cover the 19th SNIP meeting

held at San Juan, PR in April 2013.

Awarded with Young Investigator Travel Award to partially cover the 18th SNIP meeting held at

Honolulu, HI in April 2012.

Awarded with the NIDA AAPI travel fellowship to attend 2011 NIDA mini-convention on

“Frontiers in Addiction Research” at Washington, D.C. in November 2011.

Awarded with a Young Investigator Travel Award to attend 17th SNIP meeting held at

Clearwater Beach, Miami, FL in April 2011

Miscellaneous

Awarded with the Doctoral Scholarship award at University of Missouri-Kansas City for pursuing

PhD.

Awarded with a scholarship at Fairleigh Dickinson University as a foreign International Graduate

Student.

Awarded twice with Certificates by Shri Sarvajanik Pharmacy College for securing 1 st position in

Second Year B.Pharm (and 5th in North Gujarat University) and 2nd position in Third Year B.Pharm

(and 13th in North Gujarat University).

Awarded with the Certificate by GENERAL HOSPITAL, MEHSANA P.P UNIT for whole heartedly

participating in INTENSIFIED PULSE POLIO IMMUNISATION 2004-2005 for Eradication of Polio.

INDUSTRIAL EXPERIENCE

Trainee QA Officer January 2007 – June 2007

INTAS Pharmaceuticals Ltd., Ahmedabad, Gujarat, India

• Involved in In-Process Quality Control in solid drug manufacturing.

• Was actively involved in international audits from UK (MHRA) and from USA (US-FDA).

• Was promoted to the parenteral dosage form plant in 2 months of experience.

• Was promoted in the oncology department for both solid and parenteral drug dosage

forms manufactured for international market chiefly USA.

Intern Candidate May 2005 – August 2005

ASTRAL Pharmaceutical Indus., Vadodara, Gujarat, India

Closely examined all departments of pharma industry during my internship.

Contact this candidate