Technician Pvt Ltd

M ayur Gajera

****, **** ***** ***,

Mor risville, NC-27560

Cell: 717-***-****

Email: *********@*****.***

SU M MARY:

• I have more than 3 years of work experience in biopharmaceutical

organization as a research technician involved in various activities

related to cell culture such as i n vitro testing of lead molecule using

various cell lines, cell line maintenance and as a lab technician, with

substantial experience of quality testing and other research oriented

activities.

WORK EXPER I ENCE:

E r is Lifesciences Pvt L td. I ndia Jan

2012 – Sept 2013

Job title: Research Technician/Executive

• Maintained various cell lines such as A-549 (lung cancer) cell line,

M DA MB-231 (breast cancer) cell line, PC-3 (prostate cancer) cell

l ine

• Performed various cell-based assays such as cell viability assay,

cytotoxic assay, apoptotic assay and RT-PCR, gel electrophoresis

• Data collection and in terpretation of the experimental data

• Standardized cell based assay for testing molecules using different

cell lines

• Cryopreservation of cell lines and revival of the same

• Prepared culture media and stock solution of the test molecules

• Handled various lab equipments like incubator, centrifuge,

m icropipette, spectrophotometer, laminar f low hoods and

f luorescence microscope

• Responsible for accurate, fully understandable, and complete record

keeping of research work and experimental results

Azafran innovacion Pvt Ltd. I ndia

J une 2011 – Dec 2012

Job title: Research T rainee

• Performed various lab experiments such as chromatography of

natural products, pH measurement, acid-base ti t rations

• Tested lead compounds for i ts effect on cell lines

• Investigated changes in expression of regulatory proteins

• Act as QC technician for in process quality analysis

• Calibration of lab equipments such as weight balance, pH meter

• Maintaining neat and clean laboratory equipements

PROJECT EXPER I E NCE:

N I PE R-Ahmedabad J uly

2009 – June 2011

P roject title: Screening of some I ndian Medicinal Plants for Anti-

cancer Activity

• Prepared methanolic extracts from six different parts of the three

I ndian medicinal plants

• Tested for their cytotoxic ability using i n vitro cell based assays

against human cancer cell lines

• The extract with lowest IC 50 value was further fractionated.

• Lead compounds were isolated and tested for i ts effect on cancer cell

l ines

• The mechanism of action of the active molecule was determined

using different I n vitro assays like Annexin V assay and RT-PCR

and gel electrophoresis.

ED UCAT ION

B. Pha rma

Gujarat University, Gujarat, India A ug 2006

to June 2009

MS Pha rma (Natu ral P roducts)

N I PE R-Ahmadabad, Gujarat, India July

2009 to June 2011

ADD I T IO NAL SK I L LS

• Scientific li terature search through Pubmed and Preparing scientific

p resentation

• Analytical techniques like TLC, HPTLC, HPLC, Column

Chromatography

• Knowledge of In ternet data surfing and downloading the information,

E-mail and common net communication through the In ternet, Familiar

w ith the MS-OFFICE and basics of the computers

REFERENCES: Available upon request.

Contact this candidate