Data Medical

Vinod Vathipadiekal Ph.D

** ***** **., *** ****** Center, Boston, MA, 02114 Cell # +1-301-

***-**** *********@*****.***

. Fields of expertise: Expertise in Biochemistry, Cell and Molecular

Biology.

. Special Skills: Excellent capacity for leadership and

interdisciplinary teamwork in a culturally diverse environment due to

strong international experience, effective management, organization

skills and friendly personality.

. Experience in grant writing, data curation, preparing scientific reports

and grant progress reports, and presented research data in several

international conferences (oral as well as poster).

. Excellent record of publishing research articles in top-tier journals

including Cancer Cell, Lancet Oncology, Journal of Biological Chemistry,

Biological Chemistry, Peptides (total 25 publications).

Permanent resident of U.S.A, (Visa sponsorship not required)

TECHNICAL SKILLS

Cell and Molecular Biology: Standard cell and molecular biology techniques;

Genomics, Cancer Stem Cells isolation and characterization; Microarrays,

Biomarker / Target discovery and validation; Bacterial and mammalian cell

culture; Handling clinical specimens, Tissue preparation and isolation of

biomolecules; Nucleic acid preparation for genomic analysis; Plasmid

preparations; Transformation and transfection; siRNA / shRNA methods;

Establishing cell lines from primary tumors and ascites; Expression of cDNA

; Cell signaling and Signal transduction analysis.

Assay Development and Analytical methods: qRT-PCR; Immunohistochemistry;

DNA Microarray; Tissue Microarray; Flow cytometry; Western blot; ELISA;

Immunofluorescence; Development of cell and enzyme-based assays;

Colorimetric assays; PCR assays; Fluorescence spectroscopy; Microscopy;

Column chromatography; HPLC; Spectrophotometry; SDS-PAGE; Cellular

chemosensitivity assays; mRNA profiling assays to identify global genomic

changes and pathways.

Bioinformatics: Database management; Analysis of microarray data using

different bioinformatics software's including BRB ArrayTools;

Identification of pathways / biomarkers using Pathway Studio software;

Analysis of genomic data from publically available databases including

TCGA; Survival analysis; Generation of gene signatures; Identification of

targetable pathways and molecules; FlowJo, GraphPad Prism and Origin

Software; Data analysis and statistical methods.

Drug discovery and Enzymology: Biochemical Assays; Screening for drugs from

natural sources; Isolation and characterization of proteins/enzymes and

peptides from natural sources; Enzyme assay development and drug

evaluation; Enzyme kinetics; Evaluation of kinetics parameters for enzyme-

inhibitor interactions and curve fitting.

Industrial Projects: Worked for industrial research projects in

collaboration with pharmaceutical companies such as Roche and Sanofi to

identify drug response biomarkers.

PROFESSIONAL EXPERIENCE

Research Scientist, Massachusetts General Hospital, Boston, November 2011 -

present

Supervisor: Dr. Michael Birrer, MD, PhD.

. Identified and validated DNA repair enzymes such as POLH and REV3L as

biomarkers and potential drug targets, for chemoresistant ovarian cancer,

using gene expression profiling on patient samples.

. Identified novel secreted biomarkers signature for ovarian cancer

detection through genomics.

. Team member in the first personalized therapy clinical trial for the Low

grade serous ovarian cancer patient's by targeting MAP kinase pathway- A

phase II trial of selumetinib (AZD6244) in women with recurrent low-grade

serous carcinoma of the ovary (GOG239).

Post Doctoral Research Fellow, MGH Cancer Center / Harvard Medical School

(HMS), June 2009 -November 2011 / National Cancer Institute/ NIH, Bethesda,

June 2007-June2009 (Lab moved to MGH, Boston)

Supervisor: Dr. Michael Birrer, MD, PhD.

. Identified and validated a prognostic gene expression signature that

correlated with survival in serous subtype of ovarian carcinoma. This led

to the identification of a gene called MAGP2 as a therapeutic target for

ovarian cancer.

. Generated a potential ovarian cancer stem cell signature using cells

isolated from ascites of ovarian cancer patients and identified Notch

signaling as a potential therapeutic target.

. Generated a histotype specific gene signature for clear cell ovarian

cancer and identified druggable pathways involving HIF 1 alpha and

enolase-mediated metabolic pathway.

. Contributed genomic data for deposit in "The Gene Expression Omnibus",

which are approved by the GEO staff for scientific use (Accession

Numbers: GSE33874, GSE29450, GSE18521).

Doctoral Research Fellow, National Chemical Laboratory, Pune University,

India, June 2002 to May 2007

Research Guide: Dr. (Mrs.) Mala Rao, PhD

. Isolated and characterized a novel peptidic drug of HIV-1 Protease from

an extremophilic Bacillus sp.

. Evaluated the inhibitor potency and deciphered the mechanisms of enzyme

inhibition using active site labeling technique, spectrophotometry and

fluorimetric assays.

. Cloned HIV-1 Protease inhibitor (11 amino acid peptide, ATBI) using

Gateway cloning technology.

. Established an amino acid assisted catalytic mechanisms for glycosidases

for its improved performance in alkaline pH range making it suitable for

industrial applications.

. Demonstrated a method with a potential for the rapid screening of drugs

that regulate the protein expression directly from an intact cell by

laser desorption / ionization.

. Generated nanoparticle based enzyme biocatalysts and optimized assay

conditions.

COMMUNICATION AND ORGANIZATION

. Experience in presenting complex data in formal oral and poster

presentations to multidisciplinary groups from 10 to over 50 people.

. Establishing project goals and accomplishments and submission of

progress reports.

. Reviewed and published scientific manuscripts and interpreted

technical literature.

. Worked on large multi-lab scientific projects and clinical trails as

a team member. Determined project subset goals ad set schedule.

Contributed technical expertise and generated data.

. Languages: English, Hindi and Malayalam (Fluent). Tamil (basic)

LEADERSHIP AND MANAGERIAL

. Trained coworkers on technical scientific instruments.

. Supervised new students and employees in initial projects in the labs

and train them technical skills.

. Experience in setting up and maintaining research laboratory

(Exclusively involved in moving Dr. Birrer Laboratory from NCI / NIH,

Bethesda to MGH / Harvard Medical School, Boston).

EDUCATION

. Ph.D in Biotechnology, University of Pune, India, 2007.

. M.S in Biotechnology, University of Pune, India, 2002.

. B.S in Medical Biochemistry, University of Calicut, India, 2000

RESEARCH GRANTS (PEER-REVIEW)

. "Role of POLH and REV3L in ovarian cancer pathogenesis and chemotherapy

Resistance" von der Luft grant for ovarian cancer research ($50,000.00)

Role: Principle Investigator

ORIGINAL RESEARCH PUBLICATIONS:

Research articles: 25 (7 first author); Book Chapters: 2 (2 first author);

Reviews: 2 (1 first author); Selected Publications listed

. Vathipadiekal, V., and Rao, M. (2004) J. Biol. Chem. 279: 470**-*****.

. Vinod V. P., Sudheer, S., Vergenia, B., Shukla, P. G., and Rao, M. (2006)

Biotechnol. Progr. 22:1585-1590

. Vathipadiekal V., Verma, A., and Rao, M. (2007) Biol. Chem. 388: 61-65

. Vinod V. P., Phadtare, S., Joshi, H., Sastry, M., and Rao, M. (2007) J.

Nanosci. Nanotechnol. 7, 2767-2773

. Vathipadiekal V, Umasankar PK, Patole MS, and Rao M (2010) Peptides.

31:16-21.

. Mok SC*, Bonome T*, Vathipadiekal V, Bell A, et al. (2010) Cancer Cell.

16:521-532. (*Equal authorship).

. Stany MP*, Vathipadiekal V*, Ozbun L,, et al. (2011) PLoS ONE.6

(7):e21121 (*Equal authorship)

. Vathipadiekal V*, Saxena D*, Mok SC, et al. (2012) PLoS ONE. 7(1):e29079

. Farley J, Brady WE, Vathipadiekal V, Lankes HA, Coleman R, et al. (2013)

. Lancet Oncol. 14(2):134-40.

BOOK CHAPTERS

. Vinod, V. P., Phadtare, S., Sastry, M., and Mala Rao (2004) Plant,

Microbes and Biotechnology. Pointer Publishers (India) Dr. Sampat Nehra

(Editor), p- 1-13, ISBN 81-7132-398-7.

. Vathipadiekal, V and Rao, M (2011) "Nanobiocomposites for Biocatalysis":

In Encyclopedia of Nanoscience and Nanotechnology. American Scientific

Publishers. Dr. H. S. Nalwa (Editor), Vol. 16, Pages 539-549.

MANUSCRIPT READY FOR SUBMISSION:

. Vathipadiekal V, Waldron L, Wang V, Skates SJ and Birrer MJ (2014)

Creation of a Microarray which Measures the Gene Expression of Human

Secreted Proteins "Secretome Array": Validation using Ovarian Cancer Gene

Expression Data for Biomarker Discovery.

. Vathipadiekal V, Ozbun L, Lee JW, Vivas-Mejia P, Lopez-Berestein G, Sood

AK, Mok SC and Birrer M (2014) Gene expression profile of chemoresistance

of microdissected papillary serous tumors of the ovary identifies POLH

and REV3L as potential therapeutic targets.

AWARDS & HONORS

. "AACR-Bristol-Myers Squibb Oncology Scholar-in-Training Award".

. AACR 2012 Annual Meeting "Highly Rated Paper".

. "von der Luft Award for Ovarian Cancer Research" MGH Cancer Centre.

. "Poster of Distinction Award" MGH's ECOR, Boston.

. "IUBMB (International Union of Biochemistry and Molecular Biology) Young

Scientists Award".

. Travel Grant 20th IUBMB International Congress of Biochemistry and

Molecular Biology, Japan.

. "Department of Science and Technology Award" DST Govt. of India to attend

the Nobel Laureates and Students meeting in Lindau.

. "Deutsche Forschungsgemeinschaft" (DFG, Germany) invited to visit various

research institutes in Germany.

. "Best Research Scholar Award" from National Chemical Laboratory, CSIR,

Govt. of India.

. CSIR/UGC-Research Fellowship by CSIR/UGC, India.

. Qualified Graduate Aptitude Test in Engineering (GATE).

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