Technician Project Management

Ramulu Punna

**********@*****.***, Phone: 213-***-****; : 284, 2nd St, Saddle Brook, NJ-07663

Professional Summary:

● 35 years of lab and research experience in data generation and analysis. Knowledge of

general laboratory equipment and materials, GLP/GMP, ISO/IEC: 17025:2005

● Ability to work independently, record keeping and multitasking within a team environment

● Excellent written and verbal communication skills

● Expert in preparation of manuscripts and SOPs

● Proficient in Microsoft Office tools: Word, Excel and Power point

● Organized professional with exceptional project management abilities to plan and oversee

projects from concepts to successful conclusion

Work Permit: Legally authorized to work for any employer in the USA without sponsorship

Technical Skills:

Hands on experience in microbiological, clinical, histopathological, biochemical and

endocrinological examinations of blood & food samples using sophisticated techniques

Chromatography (TLC, GLC, HPLC)

Spectrophotometer (UV-Visible, Atomic Absorption & Fluorescence)

Molecular Biology (PCR & Cell Culture)

Immunology (Raising Antibodies, ELISA, Western Blot & Electrophoresis)

Endocrinology (Iodination, Radioimmunoassay, Radio receptor binding assay &Gama Counter)

Pathology (Microscope, Auto Technicon & Ultra centrifuge)

Research Skills:

Generation of data on insoluble, soluble and total dietary fiber contents of common Indian foods

and incorporated the data into National Institute of Nutrition's publication "Nutritive Value of

Indian Foods".

Isolation of soluble dietary fiber fraction, a good source of galactomannan from fenugreek seeds

Demonstration of hypoglycemic & hypocholesterolemic effects of galactomannan in obese rats

Standardization of biologically active A14125 I-Insulin by C-18 reverse phase Cartridge and HPLC

Standardization and generation of riboflavin content of commonly consumed foods by HPLC

Professional Experience:

Technical Officer, National Institute of Nutrition, Hyderabad, India 1998-2014

Analytical and Food Chemistry

Determination of insoluble, soluble and total dietary fiber contents in foods by AOAC method

Effect of maturity and processing on insoluble, soluble and total dietary fiber contents of foods

Evaluation of hypoglycemic and hypolipidemic properties of SDF (galactomannan) in obese rats

Analysis of chemical/nutritional/non-nutritional/proximate composition of various foods

Studies on protein quality/biological/safety/toxicological evaluation of foods in experimental rats

Endocrinological Techniques

Radioimmunoassay of insulin in serum and insulin receptor binding assay in erythrocytes

ELISA technique was used for serum transferitin protein binding assay

Radioimmunoassay was used for T3, T4 and TSH levels in serum

Separation of pure red blood cells from whole blood

Technical Assistant, National Institute of Nutrition, Hyderabad, India 1985-1998

Microbiological examinations of food samples

Preparation of agar media and transfer into test tubes and plates

Sterilization by autoclave and glassware in hot air oven

Sub culturing of micro organisms into new agar tubes under asepting condition

Inoculation of micro organism into microbiological assay tubes & incubation in Incubator at 370

HACCP, mycotoxin tests in foods as per GLP, ISO/IEC: 17025: 2005 and Food Safety and

Standards Authority of India (FSSAI)

Lab Technician, National Institute of Nutrition, Hyderabad, India 1976-1985

Biochemical Analysis

Biochemical assays for liver function, kidney function and water soluble vitamins in serum

Quantification of blood glucose, proteins like albumin, globulin and fibrinogen

Determination of total proteins in CSF fluid for neurological status of the body

Histopathological examinations

Biopsy of body organs, collections of organs and tissues processing by Auto Technicon using

various organic solvents at different ratios

Lab Technician, Pathology Dept. Govt. Ayurvedic Medical College, India 1974-1976

Clinical Pathological examinations

Complete urine, feces and blood examination and biochemical analysis

Education:

PhD in Biochemistry, Osmania University, India, 2001

Associate in Analytical Chemistry, Institution of Chemists (India), 1992

B.S in Biology and Chemistry, Osmania University, India, 1974

Certificates:

1. Lab Quality Management System & Internal Audit as per ISO/IEC 17025:2005 from Indian

Institute of Quality Management, Jaipur, India

2. Laboratory Technician Certificate from Institute of Preventive Medicine, Hyderabad, India

3. Certificate Course on Endocrinology Techniques from National Institute of Nutrition, India

4. Short Term Course on Biostatistics from National Institute of Nutrition, Hyderabad, India

Publications/References:

List of research publications and references will be provided up on request

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