Engineering Engineer

Stephanie Grainger, Ph.D.

** ******* ****, ********, ** 02180

Phone: (603) 566 – 0504 / Email: ********@*****.*******.***

SUMMARY:

Accomplished scientist with extensive experience in tissue engineering, biomaterials, and stem cell biology.

Proven track record of quantitative assay development, experimental study design, and achievement of focused

and collaborative results in both academic and industrial settings.

SKILLS and QUALIFICATIONS

Cell-based assays and culture techniques: cell phenotypic and functionality assays, protein expression assays,

angiogenesis models, tumor models, viability assays, stem cells, primary and immortalized cell lines

Drug delivery: synthetic microspheres, nanoparticles, growth factor delivery from hydrogels, 3D microfluidic

systems

Biomaterials: fibrin, collagen, elastin, synthetics

Animal models: in vivo implantation of biomaterials and cellularized scaffolds, microsurgical techniques,

establishment of new models, histological processing and analysis

Microscopy: widefield fluorescence, confocal, brightfield, dissecting, scanning/transmission electron microscopy

Image processing: immunofluorescence, immunohistochemistry, analysis via MATLAB and ImageJ

Mechanical analysis: uniaxial stress-strain, rheology, pressure-diameter, suture retention

PROFESSIONAL and EDUCATIONAL EXPERIENCE:

Postdoctoral Research Fellow, Department of Surgery at Harvard Medical School and Beth Israel

Deaconess Medical Center (6/2012 – Present; Advisor: Dr. Elliot Chaikof).

Developed tissue engineered vascular grafts utilizing both native and synthetic biomaterials, stem cells, and

drug delivery mechanisms.

Formed partnerships with multiple external groups to invent a method for alignment of biomaterial micro- and

nano-structures for improved in vivo graft performance.

Ph.D., University of Michigan, Department of Biomedical Engineering, Laboratory of Cell Signaling in

Engineered Tissues (8/2009 – 4/2012; Advisor: Dr. Andrew Putnam).

Produced a novel assay for quantitative assessment of tissue engineered blood vessels in 3D matrices.

Discovered differences in the behavior and performance of different types of stem cells, both quantitatively,

and mechanistically.

Lead collaboration thrusts, and obtained funding, with partner labs to develop new animal models for

assessment of this new technology in vivo.

Research & Development Intern, BioPharma Research Division, Praxair, Burr Ridge, Illinois (5/2010 –

8/2010).

Designed a new method utilizing carbon dioxide to eliminate viral contamination from bacterial cultures for

downstream pharmaceutical purification processing reductions.

Teamed with other researchers on protein purification, freeze-drying optimization techniques, and large-scale

mammalian cell culture projects.

M.S., University of Michigan, Department of Biomedical Engineering, Cellular Engineering & Nano-

Therapeutics Laboratory (9/2007 – 7/2009; GPA: 3.8/4.0; Advisor: Dr. Mohamed El-Sayed).

Created an in vitro 3D solid tumor model, and evaluated the feasibility of therapeutic ultrasound in enhancing

the delivery of polymeric drug delivery particles.

Plant Engineer, Rohm and Haas Electronic Materials, Marlborough, Massachusetts (5/2007-8/2007).

Designed a waste water recovery system for multiple pieces of equipment while working both independently

and in conjunction with other engineers and operators.

Completed purification experiments at the bench, then scaled the work up for application and successful use in

a pilot plant.

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Stephanie Grainger, Ph.D.

37 Newcomb Road, Stoneham, MA 02180

Phone: (603) 566 – 0504 / Email: ********@*****.*******.***

B.S., University of New Hampshire, Department of Chemical Engineering (9/1999 – 5/2003; GPA: 3.84/4.0)

Graduated as top student within the program. Completed 3 summers of internship work (2 listed below):

Pollution Prevention Intern, Rohm and Haas Electronic Materials, Marlborough, Massachusetts

(5/2006-8/2006, 5/2005-8/2005).

o Performed precipitation, distillation, recrystallization, and filtration experiments at the bench to

remove polymeric contaminants from solvents.

o Succeeded in saving over 29,000 kg/yr of acetone by designing a successful purification and reuse

plan over two summers of work.

SELECTED HONORS and AWARDS:

Graduate Honors and Awards

- Poster Presentation Award, Gordon Research Conference on Biomaterials & Tissue Engineering, 2011.

- Rackham Graduate Student Research Grant, University of Michigan, 2011.

- Rackham Student Travel Award, University of Michigan, 2011.

- Fellow, University of Michigan - Cellular Biotechnology Training Program, Fellowship, 2008-2011.

- 2nd Place Poster Award, Biophysical Division, College of Engineering Graduate Symposium, 2008.

- Honorable Mention, NSF-Graduate Research Fellowship Program, 2008.

- Rackham Merit Fellow, University of Michigan, Department of Biomedical Engineering, 2007-2008.

Undergraduate Honors and Awards

- Presidential Scholar, University of New Hampshire, 2003-2008.

- Outstanding Student Award, American Institute of Chemists, 2007.

- 3rd Place Student Poster Award, American Institute of Chemical Engineers National Conference 2006.

- National Record Scholar, Tau Beta Pi, 2006.

- Top Scholar in Class Frederick Waite Scholarship, University of New Hampshire, 2005 & 2006.

- Outstanding Chemical Engineering Student Award, American Institute of Chemical Engineers, 2006.

EXTRACURRICULAR and LEADERSHIP ACTIVITIES:

Mentored several undergraduate and graduate students.

Mathematics Tutor, UNH Mathematics Center (2004 – 2007)

Society of Women Engineers (2004 – Present: UNH VP 2005 – 2006, President 2006 – 2007).

Campus Band, UM, Flute (2007 – 2012).

American Institute of Chemical Engineers (2005 – 2007: UNH VP 2005 – 2006, President 2006 – 2007).

Student Leadership Council, UNH, College of Engineering and Physical Sciences (2006 – 2007).

Tau Beta Pi, NH Alpha Chapter (2005 – 2007: UNH Treasurer 2006 – 2007)

Technical Association of Pulp & Paper Industry, (2006 – 2007: UNH President 2006 – 2007).

Symphonic Band, UNH, Flute (2003 – 2007).

Undergraduate Research Conference, UNH, (2004).

SELECTED PUBLICATIONS and PRESENTATIONS:

1. Kumar VA, Caves JM, Haller CA, Dai E, Liu L, Grainger SJ, Chaikof EL, “Collagen-based substrates with

tunable strength for soft tissue engineering,” Biomaterials Science, 1: 1193-1202 (2013).

2. Kumar VA, Caves JM, Haller CA, Dai E, Liu L, Grainger SJ (presenter), Chaikof EL, “Vascular grafts generated

from collagen and elastin analogs,” Harvard Medical School Surgery Research Day, May 11, 2013, Boston, MA,

Poster Presentation.

3. Kumar VA, Caves JM, Haller CA, Dai E, Liu L, Grainger SJ, Chaikof EL, “Acellular vascular grafts generated

from collagen and elastin analogues,” Acta Biomaterialia, 9(9):8067-8074 (2013).

2

Stephanie Grainger, Ph.D.

37 Newcomb Road, Stoneham, MA 02180

Phone: (603) 566 – 0504 / Email: ********@*****.*******.***

4. Grainger SJ, Carrion B, Ceccarelli J, Putnam AJ, “Stromal cell identity governs the in vivo functionality of

engineered capillary networks formed by co-delivery of endothelial cells and stromal cells,” Tissue Engineering

Part A, 19(9-10): 1209-1222 (2012).

5. Grainger SJ, Putnam AJ, “ECM remodeling in angiogenesis” In Mechanical and Chemical Signaling in

Angiogenesis, ed. Reinhart-King C, New York, NY: Springer, 185-209 (2013).

6. Grainger SJ, Vigen M (presenter), Putnam AJ, “Engineering functional capillary networks via cell therapy”,

Biomedical Engineering Society Annual Meeting, October 26, 2012, Atlanta, GA, Oral Presentation.

7. Grainger SJ, Putnam AJ, “Assessing the permeability of engineered capillary networks”, Gordon Research

Conference, August 1, 2011, Holderness, NH, Poster Presentation.

8. Grainger SJ, Putnam AJ, “Assessing the permeability of engineered capillary networks in a 3D culture”, PLoS

ONE 6(7): e22086 (2011).

9. Grainger SJ, Putnam AJ, “Assessing the permeability of engineered capillary networks”, Upper Midwest

Biomaterials Day, May 12, 2011, Ann Arbor, MI, Poster Presentation.

10. Grainger SJ, Putnam AJ, “Assessing the permeability of engineered capillary networks”, Cellular Biotechnology

Training Program Annual Symposium, April 27, 2011, Ann Arbor, MI, Poster Presentation.

11. Grainger SJ, Serna JV, Sunny S, Zhou Y, Deng CX, El-Sayed ME, “Pulsed ultrasound enhances nanoparticle

penetration into breast cancer spheroids”, Molecular Pharmaceutics, 7(6): 2006-2019 (2010).

12. Grainger SJ, Putnam AJ, “Assessing the permeability of engineered capillary networks in a 3D culture”,

Biomedical Engineering Society Annual Meeting, October 8, 2010, Austin, TX, Oral Presentation.

13. Grainger SJ, El-Sayed MEH, “Stimuli-sensitive particles for drug delivery” In Biologically responsive hybrid

biomaterials, ed. Jabbari E, Khademhosseini A, Boston, MA: Artech Publishing, 171-190 (2010).

14. Grainger SJ, Valencia-Serna J, Sunny S, Zhou Y, Deng CX, El-Sayed MEH, “Effect of ultrasound on the

penetration of model fluorescent nanoparticles into breast cancer spheroids”, Controlled Release Society – Illinois

Student Chapter Symposium, May 29, 2009, Chicago, IL, Proceedings.

15. Grainger SJ, Putnam AJ, “Assessing the permeability of engineered capillary networks via kinetics of perfusion”,

Cellular Biotechnology Training Program Annual Symposium, April 15, 2010, Ann Arbor, MI, Poster

Presentation.

16. Grainger SJ, Valencia-Serna J, Sunny S, Zhou Y, Deng CX, El-Sayed MEH, “Effect of ultrasound on penetration

of nanoparticles into breast cancer spheroids”, Midwest Biomedical Engineering Conference, April 3, 2009, Ann

Arbor, MI, Proceedings.

17. Grainger SJ, Valencia-Serna J, Zhou Y, Deng CX, El-Sayed MEH, “Effect of therapeutic ultrasound on

penetration of nanoparticles into breast cancer spheroids”, 14th International Symposium on Recent Advances in

Drug Delivery Systems, February 16, 2009, Salt Lake City, UT, Proceedings.

18. Grainger SJ, Valencia-Serna J, Zhou Y, Deng CX, El-Sayed MEH, “Effect of ultrasound on penetration of

nanoparticles into breast cancer spheroids”, University of Michigan College of Engineering Graduate Symposium,

November 7, 2008, Ann Arbor, MI, Proceedings.

19. Grainger SJ, Farag IH, “Study of pilot plant acetone use reduction”, American Institute of Chemical Engineers

National Student Conference, November 13, 2006, San Francisco, CA, Abstract ID #78749, (2006).

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