Medical Quality Control

Curriculum Vitae

Don P. Liu, Ph.D.

**** *** ****** **, *******, ME 04605

207-***-**** (cell), 978-***-****(home)

*******@*******.***

Citizenship: USA

Summary and Major Achievements

1. Extensive training in Molecular Biology, Neurobiology, and Mouse Genetics.

2. More than ten-year experience in rational design and characterization of genetically

modified mouse models for human diseases, colony management, and process and

technology development for efficient genotyping.

3. Project management experience: worked closely with CROs on SNP genotyping, and

foundations such as ALS for aggressive colony management due to transgene instability.

Design experiments, summarize results, write reports, and propose solutions to upper

management as well as clients based on experimental data.

4. Made several libraries (cDNA expression and genomic DNA libraries), hundreds of

clones, including more than twenty sophisticated conditional gene targeting constructs.

Successfully generated several knock-out and knock-in mouse models (Arc, Tmem67,

Trpv4, dystrophin) through homologous recombination, and many transgenic lines.

Designed hundreds of allele specific PCR and qPCR assays for mouse genotyping.

5. Familiar with many current technologies such as RMCE, ZFN/Talens and CRISPR/Cas9

for more efficient production of targeted mutations and genome editing.

Professional experience

06/2002 to date Associate Research Scientist The Jackson Laboratory

Current project: working in Dr. Chengkai Dai’s lab, my project focuses on elucidating the novel

functions of HSF1 in facilitating malignant transformation and tumor maintenance in several

mouse models and cell lines.

Rational design and characterization of genetically modified mice: generated and characterized

several mouse models for neurodegenerative disorders, kidney disease, and muscular dystrophy.

Process and technology development for efficient genotyping: Implementation of qPCR for

homozygous transgenic lines, for unstable copy numbers (SOD mice), as well as trisomic mice.

Cancer Biology, St. Jude Children’s Research Hospital, Memphis, TN

01/2000-05/2002

Studied Trp53 and NMYC pathways, mechanisms of apoptosis and tumor suppressors in

neuroblastoma.

01/1997-12/1999 Neurobiology, Friedrich Miescher Institute, Basel, Switzerland

Studied in vivo function and expression of Arc during early embryo development and in mature

central nervous system, by generating transgenic, knock-out and knock-in mouse models.

08/1991-12/1996 Medical College of Pennsylvania

Constructed and screened cDNA expression libraries for novel dopamine receptors and g-

proteins.

Studied cytoskeletal protein MAP1B and the mechanism of its neuron-specific transcription.

Technical skills

Molecular Biology: Efficient use of various technologies including classical cloning and BAC

recombineering. Hands on experience in cDNA and genomic DNA library construction.

Protein expression: skillful in making expression vectors to express proteins both in prokaryotic

or eukaryotic cells.

Cell biology and others: hands-on experience with cell culture and transfection, Western blot and

immunoprecipitation, ELISA, DNase I hypersensitivity and footprinting, RNase protection

assay, DNA mobility shift, retrovirus production and transduction, RT-qPCR for expression

analysis, in situ hybridization and immunohistochemistry.

Data analysis: familiar with commonly used software packages for DNA sequence analysis,

microarray and next-gen sequencing.

Education

08/1991-12/1996 Ph.D. (Molecular Biology and Neurobiology) Medical College of

Pennsylvania, Philadelphia, PA

09/1984-07/1987 M.S. (Environmental Microbiology) Tongji Medical University, Wuhan,

P.R. China

09-1979-07/1984 M.D./B.M. Tongji Medical University, Wuhan, P.R. China

Committee Service

06/2002-03/2013 Genetic Resource Committee, The Jackson Laboratory

06/2002-03/2013 Genetic Quality Control Committee, The Jackson Laboratory

Honors and Awards

1996 Ogburn fellowship, an award and a prize of $5,000.00 for the best thesis proposal,

Graduate School of the Medical College of Pennsylvania.

1996 Listed on Who's Who among students in American Universities and Colleges of the year

Publications

Lutz CM, Kariya S, Patruni S, Osborne MA, Liu D, Henderson CE, Li DK, Pellizzoni L, Rojas J,

Valenzuela DM, Murphy AJ, Winberg ML, Monani UR. (2011) Postsymptomatic restoration of

SMN rescues the disease phenotype in a mouse model of severe spinal muscular atrophy. J Clin

Invest. 121(8):3029-41.

Workman E, Saieva L, Carrel TL, Crawford TO, Liu D, Lutz C, Beattie CE, Pellizzoni L,

Burghes AH. (2009) A SMN missense mutation complements SMN2 restoring snRNPs and

rescuing SMA mice. Hum Mol Genet. 18(12):2215-29.

Cook SA, Collin GB, Bronson RT, Naggert JK, Liu DP, Akeson EC, Davisson MT. (2009) A

mouse model for Meckel syndrome type 3. J Am Soc Nephrol. 20(4):753-64.

Dong P. Liu, Cecilia Schmidt, Tim Billings, and Muriel T. Davisson (2003) A

Quantitative PCR Genotyping Assay for Ts65Dn. BioTechniques 35, 1170-1180.

Tal Teitz, Tie Wei, Dong Liu, Virginia Valentine, Marcus Valentine, Jose Grenet, Jill M. Lahti,

Vincent J. Kidd (2002). Caspase-9 and Apaf-1 are expressed and functionally active in human

neuroblastoma tumor cell lines with 1p36 LOH and amplified MYCN. Oncogene 21:1848-1858.

Dong Liu, Di Bei, Hemar Parmar and Andrew Matus (2000) Activity-regulated, cytoskeleton-

associated protein (Arc) is essential for visceral endoderm organization during early

embryogenesis. Mech. Dev. 92:207-215.

Dong Liu (1997) Mictotubule-associated protein 1B (MAP1B) gene expression in the nervous

system: characterization of the gene promoter and the molecular mechanism regulating its

transcription. Ph.D. thesis. UMI Dissertation Services. Medical College of Pennsylvania, PA

Dong Liu and Itzhak Fischer (1997) Structural analysis of the proximal region of the

microtubule-associated protein 1B promoter. J. Neurochem. 69, 910-919.

Dong Liu and Itzhak Fischer (1996) Isolation and sequencing of the 5' end of rat microtubule-

associated protein (MAP1B)-encoding cDNA. Gene 171, 307-308.

Dong Liu and Itzhak Fischer (1996) Two alternative promoters direct neuron-specific expression

of the microtubule-associated protein 1B gene. J. Neurosci. 16, 5026-5036.

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