Maintenance Training

Resume

Mandhata Singh

(M.Sc. Biotechnology)

E-mail:- ****************@*****.***

Mob. No: - 91-995*******, 801*******

Objective :-

Aspiring for a career that places me in a challenging position within a fast paced and learning

oriented environment so as to develop my technical and inter personal skills .

Job Profile :-

I am directly involved in organ transplantation(solid organs & bone marrow) by

conducting HLA molecular typing and CDC Cross match test & its prospective study for

patient coming for organ transplantation in Transplant Immunology & Molecular

Biology Lab, Indraprastha Apollo Hospital Sarita Vihar New Delhi.

Educational Qualification :-

Course Institute University/Board Subject %age

Department of V.B.S.Purvanchal University,

Ist

M.Sc.Biotech Biotechnology, Jaunpur U.P. Biotechnology

V.B.S.P.U. Jaunpur

B.Sc. SMM Town PG V.B.S.Purvanchal University, Biotechnology,

IInd

College Ballia Jaunpur U.P. Chemistry, Zoology

Intermediate BBIC Navanagar U.P. Board Chemistry, Physics,

Ist

Ballia Allahabad Biology, English, Hindi

Highschool BBIC Navanagar U.P. Board Science, Mathematics,

Ist

Ballia Allahabad Social Science, English,

Hindi, Sanskrit

Work Experience:-

Indraprastha Apollo Hospital, Sarita Vihar New Delhi, India

Jr. Molecular Biologist, Transplant Immunology & Molecular Biology since

2013 to Present.

See whole sole of HLA Molecular Typing for Solid organs(Liver & Kidney) and

Bone Marrow Transplant

Prepared Standard operating protocol for HLA Molecular Typing.

Conducting trainings for Post Graduate (M. Tech Biotech, M.Sc. Biotech, and

Microbiology) & Under Graduate (B. Tech Biotech B.Sc. Biotech) students from

various Government and Private Institutions.

Equipment Maintenance and Library In charge

Bench strength:-

My strength lies in the bench work experience of one and half years in the field of

Transplant Immunology & molecular diagnostics during which I have done more

than 2000 HLA Molecular typing For Solid organs and Bone Marrow Transplant.

I have Processed <300 HLA –B*27 Molecular typing.

Trainings :-

I have completed Biotech Industrial Training Programme(BITP) at Auroprobe

Laboratories Ghaziyabad in the area of “ Comparative study of bactec 460 TB

system and PCR for the detection of Extra pulmonary Tuberculosis” sponsored

by DBT Government of India organized by Biotech consortium India

Limited(BCIL) New Delhi .

Dissertation work on “Diversity analysis of Halophillic & Halotolarant Fungi

from salt affected region of Kutch” from NBAIM Mau,UP-275101

Participated Three day workshop at Dept. of Biotechnology VBS Purvanchal

University Jaunpur in the supervision of Dr. Lalji Singh.

Two-month summer training from Deptt. of Biotechnology VBS Purvanchal

University Jaunpur

Techniques performed :-

A. Molecular Biology and genetics

HLA class I & class II Molecular Typing (HLA-ABDR) for solid organs (Liver, Kidney)

transplant

HLA class I & class II Molecular Typing Five locus (HLA-A B C DR & DQ) for Bone

Marrow transplant

HLA class II Molecular Typing for sarcoidosis

HLA class I (HLA-B*57:01) Molecular Typing for hypersensitivity reaction to

Abacavir

HLA-B*27 by SSP PCR

Complement Dependent Cytotoxicity (CDC) Cross Match by NIH method.

Panel Reactive antibody (PRA)

Centrifugation

Electrophoresis

DNA Isolation From Human Blood Tissue, Microbes

PCR

B. Cytogenetics

Preparation of Metaphase Chromosome

Instruments Handled:-

• Microscopy

• Thermal Cycler (Bio Rad,MJ Research & Applied Bio system)

• RT PCR

• HPLC

• Spectrophotometer

• Electrophoresis Unit

• Gel documentation system (Bio-Rad)

• And all other Instrument required in Molecular Lab

Computer Literacy:-

Operating System – Dos, Windows.

Application - MS office (MS Word, MS PowerPoint, MS Excel), Internet surfing.

Bioinformatics Software Expertise: - Database and Prediction Servers – Working

knowledge with some important bioinformatics tool & software like Primer3,

Rosmol, Primer designing

Mine Positive Aspects:-

I believe in hard work and complete honesty for my work.

Balanced temperament

Professionally committed and responsible. Adapt easily to new situations.

Successfully handled wide range of functions including, logistics and

maintenance using a combination of creative, organizational and interpersonal

skills.

Maintain focus in demanding work environments under deadline and pressure

conditions. Meet challenges head on and always find a way to effectively

complete multiple assignments or tasks.

Strong problem solving and decision making skills with the ability to develop

and implement effective action plans.

Energetic and self-motivated team player and builder. At ease in high stress,

fast paced environment with emerging and multiple responsibilities.

Punctual.

Strong communication skill

Personal Details :-

Father’s name Late Chandra Bhan Singh

3rd Aug 1989

Date of birth

Sex Male

Marital Status Single

Permanent address Vill-Husenpur, Post-Jajauly, Dist- Ballia, U P Pin: 221717

Mob. No. 91-995*******, 801*******

Hobbies Reading Book, Playing Cricket

Declaration :-

I hereby declare that the information given in this resume are true and correct to the

best of my knowledge. In case, any error is found in future. I shall be liable for the

consequences among from there including termination of my employment or withdraw of

the offer of my employment.

Date: Mandhata Singh

Place: - New Delhi

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