Research scientist with 6 yrs of job experience in glycobiology

ARYA A

‘Akshara’

Kudallur(P.O)

Palakkad(Dist)

Kerala-679554

India

Ph:-+918*********

e- mail:-****.*****@*****.***

Motivation

An enthusiastic, hardworking and adaptive person with a keen interest in research towards a more comprehensive

understanding of science, I particularly enjoy collaborating with scientist from different disciplines to develop new

skills and solve the new challenges.

Education

2005 – 2007 M.Sc Biotechnology, Bharathiar University,Coimbatore, Tamil Nadu,India.

2002 – 2005 B.Sc Biotechnology, Biochemistry and Genetics, Bangalore University,

Garden City College, Bangalore, India.

Research experience

2010- 2013 Senior Scientist, Biocon Research Limited, Bangalore, India.

• Development and qualification of N and O glycan analysis in TNFreceptor

Fc( IgG) fusion protein using chemical and enzymatic methods.

• Development and qualification of N-glycan analysis method for multiple

therapeutic monoclonal antibodies.

• Characterization and comparison of N-glycosylation in Nimotuzumab (antiEGFR

antibody marketed for head & neck cancer) expressed in CHO and NS0 cell lines.

• In vitro glyco-engineering of anti-Her2antibody and its correlation with biological

activity to study the effect of glycosylation on the biological activity of the anti-HER2

antibody.

• Glycoprotein purification using Lectin affinity chromatography and determination

of its glycan structures.

• Leadership and project management: Lead a team of three scientists and three project

trainees. Evaluation of the quality of the N-glycan, monosaccharide and sialic acid data

generated for multiple therapeutic monoclonal antibodies. Plan group experiments to

achieve the goals and meet the timelines of multiple programs.

2007-2010 Scientist, Biocon Research Limited, Bangalore, India.

• cIEF, CZE and CE-SDS method development for multiple monoclonal antibodies .

• Isolation,tagging and analysis of N-glycan from various glycoproteins by NP-

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HPLC and MALDI-MS

• Structural characterization of monoclonal antibodies by ‘Top-Bottom’ analysis

method using LC-MS.

• Intact mass determination of fusion proteins and monoclonal antibodies using

MALDI-MS.

• Sialic acid and monosaccharide analysis of various glycoproteins and their

quantitation by RP-HPLC method.

• Separation of antibody Fc and Fab domains by affinity chromatography

• Ion exchange chromatography to study the charge variants of proteins.

2006-2007 Research trainee, Characterization Development Laboratory, Biocon Limited,

Bangalore, India.

• Method development for protein analysis using capillary electrophoresis .

Description: Each protein has its own physiochemical properties which

affect its characterization parameters. I optimized sample preparation and

analytical parameters based on the protein’s physiochemical properties

using capillary zone electrophoresis .

Poster presentations

• AryaAloor, Jithendar Panda,LaxmiAdhikary, PradeepKabadi, Preetha Ramakrishnan,

Raju Mukherjee and Varshini Priya. 2011. Effect of high mannosylation on ADCC activity

of therapeutic mAbs. Indian proteomics conference, Delhi, India.

• AnandKedkar, Ann Jessita, AryaAloor,LaxmiAdhikary,

Nithyakalyani,PradeepNarayanaswamy, Raju Mukherjee, SnehaPadiyar and Susheel

Vaidya. 2009. Mass spectrometric studies of proteins.Biocon Ltd, Bangalore, India.

Training

2003 Training on microbial techniques,Sudharma laboratories, Trissur,Kerala, India.

2004 Training on biochemical techniques from Medical Trust Hospital, Eranakulam, India.

2005 Training on recombinant DNA techniques, Sreedhar Bhat laboratories, Bangalore, India.

2008 Trained on capillary electrophoresis fromBeckman Coulter, United Kingdom.

Instrument Knowledge and Hands on experience

• MALDI TOF/TOF (AutoFlex-III),BrukerDaltonics

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• ESI-MS (HCT Ultra PTM Discovery), BrukerDaltonics

• ESI-QTOF (QSTAR-XL, Applied Bio Systems)

• Agilent HPLC 1100 and 1200 series with DAD and FLD

• AKTA Prime

• Beckman coulter PA800 and PA800+ capillary electrophoresis

• Agilent Capillary electrophoresis

• UV Spectrophotometer

Hobbies

• Reading, music and traveling

Personnel Information

Gender: Female

Husband: Kannan Salim Kumar

Date of Birth: 19th April, 1985

Languages: Fluent in English and Malayalam. Can also communicate in Hindi, Kannada and Tamil.

References

Dr. Laxmi Adhikary

Principal Scientific Manager

Research and Development

Biocon Research Ltd.

Mobile: +91-984*******

e-mail: *****.********@******.***

Dr. Dinesh Palanivelu

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Associate Scientific Manager II

Research and Development

Biocon Research Ltd.

Mobile: +91-988*******

e-mail: ******.**********@******.***

Declaration

I hereby declare that the above information is correct to the best of my knowledge and I bear responsibility for its

accuracy.

Arya Aloor

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