Researcher Biochemist Research Associate

AHMED KHALIL Ahmed

*** ***** ******** **., *-**, Amherst, MA 01002

* **

Home: 413-***-**** - Cell: 413-***-****: k ***********@*****.***

Summary

Research Biochemist with ten years in laboratory research. Accomplished in generating and applying new molecular

biology protocols and biotechnologies

H ighlights

DNA, RNA, & protein isolation expert

DNA, Flow cytometry

DNA microarray technology (sample preparation)

DNA Production of antibodies

Western blotting Anti-cancer natural products assessment

cancer

Down-stream bioprocessing Enzyme activity assays

Expert in mammalian cell culture Yeast gene expression

ELISA & Immunoassays Seahorse Technology

Expert in animal colony and modeling

Fluent in English and German

Fluent

Experience

Research Associate

2011 - 2013

Bay state Medical Center- University of Massachusetts - Springfield, M A

University

Setting up successfully different methods to investigate the mitochondrial dysfunction in cancer, diabetes, and

neurological diseases using Cell culture, Western Blotting, Seahorse technology to monitor metabolic activities, PCR,

neurological

genotyping and animal modeling of complex I deficiency.

of

Biochemist

2010 - 2011

Food Science Dep. - University of Massachusetts - Amherst, M A

Studied

Studied the therapeutic effect of conjugated linoleic acid on prostate cancer using Cell culture viability tests and

Flow cytometric analysis.

Research Fellow

May 2010 - Aug.2010

Medical Biochemistry Dep. - Uppsala University - Uppsala, Sweden

Uppsala

Developed a quick method to examine the differences in the intracellular levels of polyadenylation factors which

Developed

regulate HPV-16 late gene expression in transfected Hela cells using CAT ELISA.

Researcher and Lecturer

2008 - 2010

Genetic Engineering and Biotechnology Research Institute - Alexandria, Egypt

Setting up different protocols to investigate the antiviral effect of some Egyptian natural products on HCV infected

nvestigate

HepG2 cultures.

Researcher & Teaching assistant

2004 - 2008

Institute of Technical Chemistry - Hannover University - Hannover, Lower Saxony, Germany

Developed oligonucleotide chips to simply monitor the gene expression of Saccharomyces cerevisiae in continuous

chips

cultures using DNA micro-array technology, RNA expression level, flow cytometry and downstream processing.

Researcher and Lecturer Assistant

1997 - 2004

Genetic Engineering and Biotechnology Research Institute - Alexandria, Egypt

Engineering

Duties: Preparation, production and characterization of antitetantus antibodies using chromatographic

techniques, ELISA, SDS-PAGE and in-vivo Toxicity tests, in addition of animal (mice & sheep) colony, breeding as

bioreactors for antibody production.

E ducation

P h.D : Biochemistry, 2008

Institute of Technical Chemistry, Hannover University Germany

Dissertation Topic: A Study of Gene Expression of Saccharomyces Cerevisiae in Oscillating Continuous Cultures

Dissertation

Using DNA Microarray Technology

Master of Science: Biochemistry, 2002

AinShams University - Cairo, Egypt

Dissertation Topic: Production and Characterization of Antitetanus Fab Antibodies in sheep

Bachelor of Science: Biochemistry, 1995

Alexandria University Egypt

P ublications

● Kim, C., Patel, P., Brown, M., Khalil, A ., Yadava, N. (2014). Comparative analysis of the

mitochondrial physiology of pancreatic β cells. Bioenergetics 3:110.

● Yadava, N., Khalil, A., Schneider, S. (2013). Oxphos defects and their role in cancer initiation and progression in

.,

Mitochondria as Targets for Phytochemicals in Cancer Prevention and Therapy (Editor: D. Chandra; Publisher: Springer,

Mitochondria

New York), Chapter 1: 1-26.

● Khalil, A. (2008) A Study of Gene Expression of Saccharomyces Cerevisiae in Oscillating Continuous Cultures Using

(2008)

DNA Microarray Technology. Doctoral Dissertation. Hannover University, Worldcat.org.

● Redwan, M., Khalil, A., El-Dardiri, Z. (2005). Purification of ovine antitetanus tetanus antibody. Comparative

Dardiri,

Immunology, Microbiology & Infectious Diseases 28, 167-176.

Redwan, M., Khalil, A., El-Dardiri, Z. (2003). Production and optimization of ovine antitetanus antibody

Dardiri,

(postersession). Human Antibodies 12, 37–50.

● Clerc, P., Bordt, E., Saladino, A., Khalil, A., Yadava, N., Polster, B. (2014). Reversible off-target inhibition of

target

mitochondrial complex-I dependent respiration by the putative Drp-1 antagonist Mdivi-1(In press).

References ●

Prof. Dr. Nagendra Yadava, Assistant Professor, Pioneer Valley Life Sciences Institute, Springfield & Department of

Assistant

Biology, University of Massachusetts, Amherst Tel: 413-***-****, Fax: 413-***-****,

Email:********.******@**************.***

Email:********.******@**************.***

Dr. Adel Abd El-maksoud, Senior Researcher, Department of Chemistry, University of Massachusetts, Amherst, USA. Tel:

Department

413-***-****, E-mail: adel @chemistry.umass.edu or *******@*******.***

mail:

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