Immunology, Immunotherapeutics, biomarkers, Scientific writer, editor

*

NIVEDITA LAHIRI

*** ******* ********, ****** ** 06883 203-***-**** **************.****@*****.***

OBJECTIVE: Post-doctoral scientist and adjunct faculty seeking full time, permanent position in academia/ industry or

clinical research organizations

RESIDENT STATUS: Permanent resident (Green card holder), requiring no sponsorship.

RELEVANT QUALIFICATIONS

• PhD with more than 3 years of research experience in Harvard Medical School

• 5+ years of expertize in proteomics : Western Blot, SDS-PAGE, etc (see below)

• 5+ years of experience in handling human and murine cells.

• 3+ years of experience in Mass Spectrometry (HPLC ESI-QTOF/ LC-MS, elementary MALDI, TLC, Si Gel and

column chromatography)

• Modern bio-analytical software (see research skills section)

• Experience in working with hazardous materials, solvents, radiochemicals, BSL-2 labs.

• Editorial and reviewing experience (1 year), active scientific writer

WORK EXPERIENCE

1. Adjunct Lecturer Jan 2014-present

Dept. of Biology

Sacred Heart University

5151 Park Avenue, Fairfield CT 06825

RESPONSIBILITIES:

• General Biology laboratory courses and lectures at Bachelor’s level

• Organizing quizzes, pre-lab and in-lab assignments

• Project mentorship

2. Technical Editor (part-time) June 2013-present

Vedic Research International Inc.

1975 Washington Ave

New York, NY 11783

RESPONSIBILITIES:

• Reviewing, editing and proof reading manuscripts from research laboratories around the world.

• Scientific writing: writing articles and reviews on current scientific issues.

3. Post-doctoral fellow Jan 2009- Mar 2012

Div. of Rheumatology, Immunology and Allergy

Brigham and Women’s Hospital, Harvard Medical School

2

Boston MA

RESPONSIBILITIES:

• demonstrated the biochemical remodeling of the cell wall of rifampin resistant Mycobacterium

tuberculosis by HPLC ESI QTOF (Agilent 6520)- LCMS

• Experienced in LCMS/MS

• Bio-analytical software: Mass Hunter, R, XCMS, Mass Profiler professional, Genespring, Genepattern

• Biomarker research: identification of TB specific biomarkers from lipids extracted from urine, serum

and sputum of TB patients (by LCMS)

• Preliminary MALDI-TOF (not extensive experience)- only to detect contaminants in glycolipids

isolated from urine of TB patients, which would interfere with true biomarker detection.

4. Graduate research fellow (PhD Candidate) Dec 2002- June 2008

Dept. of Microbiology

Bose Institute, India

RESPONSIBILITIES

• Protein characterization and quantification: SDS PAGE, Western blotting, quantification by UV-VIS

spectrometry.

• Detecting and quantifying soluble proteins by ELISA and RIA

• Detecting cell surface proteins by Confocal microscopy

• Detecting nuclear proteins by EMSA

• Detecting membrane bound proteins and bound proteins by co-immunoprecipitation

• Enzyme assays

PROJECT AND RESPONSIBILITIES:

5. Lecturer Feb 2002-Dec 2002

Dept. of Physiology

St. Paul’s Cathedral Mission College

Kolkata, India

RESPONSIBILITIES: Taught theory and practical classes in Physiology and Microbiology at the bachelor’s

level; set academic curricula, question papers and checked assignments.

RESEARCH EXPERIENCE AND SKILLS

o Mammalian Cell culture: isolation and culture of murine primary peritoneal and splenic

macrophages and bone marrow derived cells; human PBMC and neutrophils

o Extraction of lipids from human samples and bacteria

o Immunological assays and cell based assays : FACS (basic), Confocal miscroscopy (basic),

ELISA, RIA, Western blot, T cell proliferation assay, Intracellular Cytokine staining (ICS), Co-

immunoprecipitation, subcellular fractionation; Cell viabiltity testing (MTT assay, trypan blue)

o Antibody production and purification from mycobacterial antigens

o PROTEOMIC STUDIES:

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o Enzymatic assays and Protein quantitation assays- UV spectroscopy, colorimetric assays,

endogenous phosphorylation of proteins, serine-threonine and tyrosine kinase and

phosphatase assays, Protein Kinase C assay, NADPH oxidase assays (DCFDA stain),

caspase activity assays, etc.

o Receptor mediated signaling

o Study of protein-protein and DNA-protein interactions: Co-immunoprecipitation, western

blotting and EMSA (electrophorectic mobility shift assay)

o Detection of protein and lipid biomarkers from TB patients’ body fluids: LC-MS and

bioanalytical software

o Molecular biology techniques: isolation of genomic DNA and RNA, EMSA, RT-PCR, qPCR, siRNA

transfection of cells, DNA fragmentation

o Animal handling: Mice. Administration of subcutaneous, intracardial and intraperitoneal injection of

drugs.

o Organ harvesting: Spleen, lungs, liver, lymph nodes for the study of cytokine mRNA

o LC-MS (HPLC QTOF Agilent 6520 series); MSMS,

o structure determination by collisional MS (MSMS);

o Lipid profiling;

o Extraction and purification of lipids from Mycobacterium tuberculosis, human urine and plasma

SOFTWARE SKILLS

• General: MS Office (Word, Excel, Powerpoint, outlook); Adobe photoshop, Adobe Illustrator, Graph Pad prism-- >5 years,

Blackboard

• Analytical: Mass Hunter; Mass Profiler Professional (Agilent), XCMS using R, Genepattern, genespring (basic) 3 years,

Basic SAS

• Special: Gel doc; Chemdraw

AWARDS AND HONORS

• CSIR-UGC-NET (National Eligibility Test for Junior Research Fellowship and Lectureship

• Nominated for Shyama Prasad Mukherjee (SPM) fellowship award for standing within the top 20% amongst the

NET candidates in life sciences (Jun 2002 and Dec 2002).

• Junior Research Fellowship of the Council of Scientific and Industrial Research (CSIR), Govt. of India,

• Senior Research Fellowship, CSIR

• Sir Nil Ratan Sircar memorial prize for pre-doctoral research work during Ph.D (2007)

EDUCATION

• PhD in Immunology and cell signaling, Bose Institute, Kolkata, India, with minor in Molecular Biology and

Biochemistry

• MS in Physiology, University College of Science and Technology, Kolkata, India,

• BS in Physiology, Presidency College, Kolkata, India

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PUBLICATIONS (arranged based on the position of authorship; *Majumder is the maiden surname of Lahiri)

1. *Majumder N, Dey R, Mathur RK, Datta S, Maitra M, Ghosh S, Saha B, Majumdar S. 2006. An Unusual Pro-

inflammatory Role of Interleukin-10 Induced by Arabinosylated Lipoarabinomannan In Murine Peritoneal

Macrophages. Glycoconj J. 23(9): 675-686

2. Majumder N, Bhattacharjee S, Bhattacharyya (Majumdar) S, Dey R, Guha P, Pal NK, Majumdar S. 2008.

Restoration Of Impaired Free Radical Generation And Pro-Inflammatory Cytokines by MCP-1 in Mycobacterial

Pathogenesis. Scand J Immunol 67: 329-339

3. Majumder N, Bhattacharjee S, Dey R, Bhattacharyya (Majumdar) S, Pal NK, Majumdar S, Arabinosylated

Lipoarabinomannan Modulates The Impaired Cell Mediated Immune Response In Mycobacterium tuberculosis

H37Rv Infected C57BL/6 Mice. Microbes Infect 10 (4): 349-357

4. Majumder Lahiri N, Das S, Bhattacharjee S, Gupta G, Bhattacharyya (Majumdar) S, Majumdar S. 2013. TLR-2

Signaling as a target for Ara-LAM mediated protection in Man-LAM induced pathogenesis. J. Biomed. Sci, 2 (2:3)

doi: 10.3823/1018

5. Lahiri N., Shah Rupal R. Murray Megan B., Fortune Sarah M. and Moody D. Branch, Unbiased Lipidomics of

Rifampin Monoresistant Mycobacterium Tuberculosis Strains Identifies Downregulated Siderophore Production.

Submitted 2013.

6. Lahiri N, Lahiri S.K. Envisioning the reversal of oncogenesis. Review. 2013. VRI Cell Signaling 1: 34-48 doi:

http://dx.doi.org/10.14259/cs.v1i2.64

7. Dey R, Majumder N, Bhattacharjee S, Bhattacharyya (Majumdar) S, Banerjee R, Ganguly S, Majumdar S. 2007.

Leishmania donovani Induced Ceramide As The Key Mediator of Akt Dephosphorylation In Murine Macrophages:

Role of PKC- And Phosphatase. Infect Immun 75: 2136-2142

8. Dey R, Majumder N, Bhattacharyya (Majumdar) S, Bhattacharjee S, Banerjee S, Roy S, Majumdar S. 2007.

Induction of host protective Th1 immune response by chemokines in Leishmania donovani infected BALB/c mice.

Scand J Immunol 66: 671-683

9. Bhattacharjee S, Majumder N, Bhattacharya P, Bhattacharyya (Majumdar) S, Majumdar S. 2007.

Immunomodulatory role of Arabinosylated Lipoarabinomannan on L. donovani infected murine macrophages:

Generation of protective immune response. Ind J Biochem Biophys 44: 366-372

10. Dey R, Sarkar A, Majumder N, Bhattacharya S, Roychoudhury K, Bhattacharyya S, Roy S, Majumdar S. 2005.

Regulation of impaired PKC signaling by chemokines in murine macrophages during visceral leishmaniasis. Infect

Immun. 73(12): 8334-44

11. Bhattacharyya (Majumdar) S, Dey R, Majumder N, Bhattacharjee S, Majumdar S. 2007. A novel approach to

regulate experimental visceral leishmaniasis in murine macrophages using CCR5 siRNA. Scand J Immunol 67:

345-353.

12. Dey R, Ghosh S, Bhattacharjee S, Majumder N, Majumdar S. 2007. Ceramide as the chief villain in the

perspective of visceral leishmaniasis: its role as the platform for pathogenic establishment. Review. Science and

Culture 73 (5-6).

PATENT

• Majumder N, Majumder S. A process for preparing pure arabinosylated lipoarabinomannan effective against tuberculosis and

leishmaniasis. 2010. Patent application No.772/KOL/2005, Govt. of India. Patent no. 237901

BOOK CHAPTER

• Das D, Saha A, Das S, Majumder N, Palchaudhury S, Majumder S, Dey S. 2005. Danger of heavy metal toxicity: Effect of

lead on male reproductive system. Book Chapter. Combating Disaster, acb publications, Section I: 27-32. ISBN 81-87500-29-8.

CONFERENCE PRESENTATIONS

• Oral Presentation: “Dual Role of Interleukin-10 in regulating the cell-signaling mechanisms of murine peritoneal macrophages:

Effect of Mannosylated and Arabinosylated Lipoarabinomannan.” Society of Biological Chemists (SBC) meeting at Digha April 9-11,

2004

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• A poster presentation: “Mannosylated and Arabinosylated Lipoarabinomannan differ in IL-10 mediated signaling in murine

macrophages”: Immunological Society Conference, PGIMER, Chandigarh Nov 24-27, 2005

• Oral presentation: “MCP-1 as a therapeutic tool against the pathogenesis induced by Mannosylated Lipoarabinomannan from

virulent Mycobacterium tuberculosis in murine macrophages.” Annual meeting of Society of Biological Chemists (SBC, Digha, May

19-21, 2006

• Poster presentation: “Arabinosylated Lipoarabinomannan modulates the impaired cell mediated immune response in

Mycobacterium tuberculosis H37Rv infected C57BL/6 mice”. International Conference on Frontier Researches in Integrative

Physiology (ICFRIP), Kolkata, from Jan 8-10, 2007.

• Poster presentation and invited oral Presentation: “MCP-1 confers protection against Mycobacterial pathogenesis by the

restoration of both free radical generation and pro-inflammatory cytokines”. 33rd Indian Immunology Conference, All India Institute of

Medical Sciences (AIIMS), New Delhi, from Jan 28-31, 2007

• Poster Presentation: Unbiased Lipidomics of Rifampin Monoresistant Mycobacterium Tuberculosis Strains Identifies

Downregulated Siderophore Production. Fifth New England TB Symposium, Cambridge MA, June 23, 2011

You can find more about me at:

LinkedIn url: http://www.linkedin.com/in/niveditalahiri/

Twitter url: twitter.com/LahiriNivedita

VisualCV: www.visualcv.com/niveditalahiri

Google+:https://plus.google.com/u/0/+NiveditaLahiri08/

Monster: http://www.beknown.com/nivedita-lahiri

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