TEFERA

A.

BOKU,

B.Sc.,

MT

(ASCP)

§

C:

*03-

475-

6314

§

ace1w1@r.postjobfree.com

MEDICAL

TECHNOLOGIST

“Producing

Accurate

Laboratory

Results

for

Timely

Diagnosis,

Treatment

&

Discharge

Decisions”

ASCP

certified

laboratory

professional

with

two

college

degrees,

offering

five

years

of

progressive

diverse

laboratory

expertise

both

in

high

complexity

clinical

laboratory

and

biomedical

research

for

evidence

based

&

patient

focused

test

results;

Self

motivated

with

strong

understanding

of

diagnostic

laboratory

procedures

(SOPs),

reference

ranges,

and

ability

to

identify

and

document

quality

control

(QC)

issues

and

activities;

Proficient

in

prioritizing

multiple

laboratory

operations;

Poised

professional

demeanor

under

stress,

impeccable

work

ethics,

adaptable

to

new

training

&

technology,

and

productive

in

team

or

independent

work

environment

CORE

LAB

AUTOMATION

AND

TECHNOLOGY

SKILLS

Sysmex

XE

2100

Hematology

Analyzer

• Dimension

Vista

1500

Analyzer

• AutionMax

AX-

4280

Urine

Chem

Analyzer

•

Sysmex

SP-

1000i

Automated

Smear

System

• ADVIA

Centaur

XP

Immunossay

• iQ200

Autom.

Urine

Microscopy

System

•

Stago

STA-

R

Mechanical

Detection

System

• GEM

4000

Blood

Gas

System

• Uricon-

NE

Refractometer

•

PFA-

100

Platelet

Function

System

•

HISTOCOMPATIBILITY

AND

MOLECULAR

TECHNOLOGY

SKILLS

BD

FACSCalibure

• OpenGene

HIV

Seq.

System

• Cobas

4800

•

BD

HLA-

B27

• COBAS

Ampliprep

• ABBOT

m2000sp

•

UNOS

• Qiagen

Rotor-

Gene

3000

• Nuclesens

EasyMAG

•

BD

Multitest

• AMPLICORE

(CT/NG)

• Various

Thermal

Cyclers

•

Roche

LightCycler

• Biorobot

EZ1

•

BLOOD

BANKING

AND

TRANSFUSION

MEDICINE

SKILLS

• ABO/Rh

Typing

• RBC

Phenotyping

• Cord

Blood

• Antibody

Screen

&

ID

• Massive

Trans.

Procedure

• Transfusion

Reaction

Workup

• Crossmatching

• Pediatric

Aliquot

&

Irradiation

• Ortho

ProVue

Technology:

Cerner

Millennium

(LIS),

EasyLink

Data

Management,

EP

Evaluator

(EE9),

Rotor

Gene

300,

Primer

3,

NCBI

database,

60

wpm,

Word,

Excel,

PowerPoint,

Access,

Outlook

Exchange,

Browsing,

Windows,

Mac,

Linux

platforms,

GraphPad

Prism,

UV-

Probe,

Softmax

Pro,

Gaussian,

Molden,

ISIS

Draw

Leadership:

Team

Collaboration,

Conflict

Resolution,

Vision,

Value

&

Goal

Focused

Synergy

PROFESSIONAL

SUMMARY

MEDICAL

TECHNOLOGIST

(ASCP)

/

MOLECULAR

ONCOLOGY

02/2013

–

04/2013

Quest

Diagnostics

–

Chantilly

Hours

:

40

14225

Newbrook

Dr,

Salary

:

$58,000.00

Chantilly,

VA

20151

(703)

802-

6900

Supervisors/

Lab

Managers/

Technical

Director

:

Christina,

Bruce,

Dr.

Quilu

Pan

Managed

an

excellent

balance

of

accuracy

and

speed

in

high

volume

testing

while

flexibly

covering

up

to

12

hour

shift

to

complete

the

task

at

hand.

• Completed

and

recorded

routine

quality

control

checks,

daily

and

weekly

preventive

maintenance

before

processing

patient

specimen.

• Processed

molecular

oncology

samples,

isolated

RNA

from

plasma

using

ABBOT

m2000sp

and

total

nucleic

acid

from

plasma

using

Nuclesens

EasyMAG

extraction

technique

• Completed

RT-

PCR

for

JAK2

V617,

JAK2

Exon

12,

JAK2

Exon

13

and

MPL

mutation

detection

and

analysis

• Extracted

DNA

using

BioRobot

EZ1

from

heparinized

blood

for

JAK2,

MPL,

IGH

B-

cell,

and

TCR

gamma

T-

cell

gene

rearrangement.

• Performed

amplification

reaction

for

B-

RAF,

PAX8/PPAR,

Ret/PTC1,

Ret/PTC3,

JAK2,

MPL,

IGH

B-

cell,

TCR

gamma

T-

cell,

immunoglobulin

VH,

HRAS,

KRAS,

and

NRAS

mutation,

translocation

or

gene

rearrangement

detection

and

analysis.

• complies

with

proficiency

surveys,

continuing

education,

performance

evaluation

and

professional

development

requirements.

TEFERA

A.

BOKU,

B.Sc.,

MT

(ASCP)

PAGE

TWO

MEDICAL

TECHNOLOGIST

HLA

/HISTOCOMPATIBILITY

TECHNOLOGIST

(ASCP)

08/2011

–

10/2011

Georgetown

University

Hospital

Hours

:

40

110

Irving

Street

NW

Salary

:

$51,000.00

Washington,

DC

20010

(202)

444-

2819

Training

Supervisor,

Jing

Guang

;

Lab

Manager:

Sharon

Novak

Excellent

performance

and

diverse

expertise

in

HLA

diagnostic

laboratory

procedures

(SOPs)

in

high

complexity

clinical

laboratory

environment.

• Isolated

class

I

and

class

II

cells

from

peripheral

blood,

lymph

node,

spleen

specimen

and

enumerated

cells

using

hemocytometry.

• Accurately

completed

serologic

HLA

typing

by

CDC

method

with

utmost

attention

to

detail

while

recording

test

results

and

protecting

patient

privacy

at

all

times.

• Performed

flow

cytometric

HLA

crossmatch

testing

on

donor

cells

and

recipient

serum,

analyzed

crossmatch

data,

documented

and

reported

hsitocompatibility

test

results.

• Knowledgeable

in

HLA

antibody

screening,

identification,

titer

as

well

as

SSP

and

SSO

PCR

molecular

HLA

typing

technique.

• Dependable

in

compatibility

analysis,

reviewing,

verifying,

releasing,

and

recording

all

test

results.

• Prevented

testing

error,

troubleshoot

failed

tests,

performed

routine

maintenance,

calibration,

quality

control,

and

function

test

• Attended

HLA

continuing

education

seminars,

completed

mandatory

trainings

and

weekly

lab

meetings.

MEDICAL

TECHNOLOGY

&

QA

SAFETY

EDUCATION

08/2010

–

08/2011

Washington

Hospital

Center

Hours

:

40

110

Irving

Street

NW

Salary

:

$26,000.00

Washington,

DC

20010

Lab

Manager:

Dr.

John

C.

Rees

(301)249-

5924

/

Program

Director:

Amy

M.

McCarty

(202)877-

3346

Consistently

commended

for

outstanding

performance

and

collaboration

in

multifaceted

laboratory

operations

including

the

evaluation

of

new

SOPs

and

daily

laboratory

operations

in

Dc’s

Largest

900

bed

hospital.

Protected

patient

privacy

at

all

times,

adheres

to

safety

resources,

policies,

CLIA,

FDA,

CAP

&

OSHA

regulatory

guidelines,

and

demonstrated

high

level

of

initiative

in

laboratory

process

improvement

through

Lean

Six

Sigma.

• Conducted

hematology,

urinalysis,

coagulation,

&

chemistry

diagnostic

testing

as

a

generalist

and

delivered

timely

and

quality

results

in

routine

&

specialty

lab

tests

from

blood,

serum,

urine,

CSF,

body

fluids,

and

other

specimens.

• Exceeds

established

turnaround

times,

reports

STAT

and

critical

results

according

to

lab

procedures

and

dependable

in

specimen

handling

and

processing,

analysis,

reviewing,

verifying,

releasing,

and

recording

all

test

results.

• Prevents

collection,

clerical,

specimen

processing

and

testing

error

and

troubleshoots

failed

tests;

performs

routine

maintenance,

calibration,

quality

control,

function

test,

method

validation,

and

instrument

correlation

to

prevent

test

result

discrepancy.

• Maintains

appropriate

level

of

lab

supplies

&

reagents

and

labels

testing

kits,

reagents

with

expiration,

open,

and

receipt

dates.

• Professionally

communicates

panic

values

and

flags

with

physicians

and

technical

information

and

laboratory

incidents

with

laboratory

managers

or

workmates

for

smooth

and

efficient

lab

operation.

• Analyzes

broad

screening

automation

tests

for

metabolic

panels,

function

tests,

markers,

lipid

profile,

TDM

&

drug

screening,

vitamins

&

minerals,

hepatitis,

HgA1c,

ABG,

CSF

protein,

and

ketone

studies.

• Performes

macro/microscopic

&

automated

dipstick

urinalysis,

pap

stain

cytodiagnostics,

clinitest,

icto

test,

acetest,

SSA,

hemastix

confirmatory

tests,

crystal,

cast,

cell,

yeast,

and

bacteria

identification

and

quantification.

• Proficient

in

routine

&

specialized

coagulation

tests,

phlebotomy

procedures

and

other

specialized

lab

tests

such

as

electrophoresis,

osmolality,

fibronectin,

and

Vit

D

• Completed

160

hours

of

hematology

bench

on

manual

CBC

and

differential

analysis,

RBC

morphology

&

coloration,

WBC

special

stains,

reticulocyte

count,

phase

platelet

count,

manual

CSF

&

body

fluid

cell

count

and

differential,

malaria

&

crystal

examination,

sickle

solubility

test,

ESR

(Westergren

Tube

Method),

and

manual

HCT.

• Complies

with

proficiency

surveys,

continuing

education,

performance

evaluation

and

professional

development

requirements.

• Performed

DNA

extraction

from

heparinized

peripheral

blood

and

observed

nucleic

acid

amplification

for

detecting

heterozygous

or

homozygous

hereditary

thrombotic

disorder

(FII,

MTHFR,

FV

Lieden

mutation)

by

melting

curve

analysis

• Trained

on

quantization

of

circulating

HCV

and

HIV

viral

load

using

real-

time

PCR

for

monitoring

therapeutic

efficacy

and

HIV

DNA

sequencing

to

detect

mutations

associated

with

resistance

to

antiretroviral

treatment

• Observed

detection

of

Chlamydia

trachomatis

and

Neisseria

gonorrhoeae

infection

from

urine,

endocervical

and

urethral

swabs

by

DNA

amplification

and

colorimetric

technique

using

HRP

labeled

probes.

TEFERA

A.

BOKU,

B.Sc.,

MT

(ASCP)

PAGE

THREE

MEDICAL

TECHNOLOGIST

MOLECULAR

AND

BIOCHEMISTRY

RESEARCH

ASSISTANT

08/2007

–

08/20010

Physiology

and

Biophysics

Dept,

Howard

University

Hours

:

40

520

W

Street

NW

Salary

:

$31,000.00

Washington,

DC

20059

Primary

Investigators

Dr.

Serdia

O.

Mack

(202)806-

9708

/

Dr.

Ayele

Gugssa

(202)806-

6783

PROJECT

ONE:

HYPOTHALAMIC

CONTROL

OF

ENERGY

EXPENDITURE

AND

BREATHING

In

studying

the

ventillatory

and

orexigenic

effect

of

neuropeptide-

Y

(NPY),

model

animals

brain

are

bilaterally

injected

with

NPY-

SAP

or

Saporine

and

physiologic

and

molecular

data

are

compared.

I

contributed

as

a

molecular

research

support

by

measuring

corticotrophin

releasing

hormone

(CrH)

and

thyroid

releasing

hormones

(TrH)

mRNA

level

and

by

comparing

qPCR

data

between

the

experimental

and

control

animal.

• Developed

new

protein,

DNA,

and

RNA

triple

extraction

procedure

from

neuropeptide

Y-

saporine

and

saline

injected

flash

frozen

rat

paraventricular

nucleus

(PVN)

tissue

for

real

time

-

RT

PCR

and

protein

quantitative

studies.

• Utilized

NCBI

gene

sequence

BLAST

search

for

TrH,

CrH,

and

control

gene

with

Primer

3

application

for

custom

primer

design

for

a

two

step

real

time-

RT

PCR

mRNA

study.

• Determined

RNA

extraction

purity

by

260

to

280

absorption

ratio

and

completed

reverse

transcription

using

MMLV

reverse

transcriptase,

DNTPs,

and

oligod

(dT),

random

and

custom

primers

on

Quiagen

kit.

•

Amplified

cDNA

using

FastStart

SYBR

Green

technology

for

semi

quantitative

real

time-

PCR

on

Rotor-

Gene

3000

and

computed

melting

curve,

and

dCT

data

analysis.

• Performed

agarose

gel

electrophoresis

and

densitometric

analysis

for

PCR

product

variation

on

treated

and

control

animal

brain

tissues.

PROJECT

TWO:

AUTONOMIC

CONTROL

SYSTEMS,

AGING

AND

OXIDATIVE

STRESS

Reactive

oxygen

species

(ROS)

is

generated

during

anomalous

oxidative

phosphorylation

process

in

the

electron

transport

chain

(ETC)

and

prolonged

ROS

attack

leads

to

age

related

snowballing

health

disorders.

I

determined

spatial

and

temporal

differential

ROS

generation,

using

genetically

encoded

biosensor

(HyPer),

and

other

selective

ROS

probes.

I

also

compared

and

characterized

antioxidant

enzyme

expression,

behavioral

variation

and

functional

senescence

analysis.

•

Standardized

quantitative

analysis

on

Drosophila

melanogaster

SOD,

CAT,

and

GST

using

microplate,

gel

activity,

western

blot

&

densitometry

to

determine

organismal

redox

temporal

homeostasis.

• Completed

model

animal

behavioral

analysis

including

locomotion,

life

span,

mortality,

fertility,

oxidative

stress

resistance

(paraquat

&

H2O2)

and

caloric

restriction

studies

for

age

related

measurable

functional

senescence.

• Contributed

in

the

quantitative

and

qualitative

determination

of

both

in

vivo

and

in

vitro

cellular

reactive

oxygen

species

(ROS)

using

HyPer

(H2O2

biosensor)

and

other

selective

probes

combined

with

micro-

scale

dissection

and

microcopy.

• Studied

and

discussed

ROS

related

biological

and

molecular

events

such

as

cell

death,

myopathy

and

nerodegeneration

which

manifests

itself

in

mutagenesis,

carcinogenesis,

diabetes,

inflammation,

and

other

age

related

health

disorders.

• Analyzed

data

for

statistical

significance

using

GraphPad

Prism

and

Excel

and

contributed

such

observation

as

a

preliminary

data

in

a

research

proposal

and

other

research

papers

for

publication.

• Presented

progress

reports,

observations,

new

research

tools,

and

related

publications

on

weekly

laboratory

meetings.

MOLECULAR

RESEARCH

ASSISTANT

10/2005

–

12/2006

Plant

Pathology

and

Physiology

Dept.,

Virginia

Tech.

Hours

:

20

551

Latham

Hall

Salary

:

$10,400.00

Blacksburg,

VA

24061

Primary

Investigator:

Dr.

Boris

Vinatzer

(540)231-

2126

PROJECT:

EVOLUTION

OF

PRE-

AGRICULTURAL

PLANT

PATHOGEN

TO

AGRESSIVE

INVADERS

OF

MONOCULTURE

CROPS

I

participated

in

DNA

sequencing

and

molecular

characterization

of

Pseudomonas

syringae

in

the

Plant

Pathology

and

Physiology

Department

in

Virginia

Tech

as

a

molecular

research

assistant.

This

investigation

entails

the

virulent

gene

acquiring

mechanism

of

wide

plant

host

range

infecting

pathogens.

I

performed

amplification

reaction,

DNA

extraction,

gel

electrophoresis,

imaging,

and

other

molecular

techniques

as

well

as

growing

and

inoculation

of

plant

models.

TEFERA

A.

BOKU,

B.Sc.,

MT

(ASCP)

PAGE

FOUR

MEDICAL

TECHNOLOGIST

Inoculate

tobacco,

tomato

and

arabidopsis

plants

with

different

strains

to

study

virulent

gene

acquiring

mechanisms

•

Assisted

in

DNA

sequencing

of

P.

syringae

by

molecular

techniques

by

designing

primer,

PCR,

electrophoresis

&

gel

imaging

•

Maintained

arabidopsis,

tomato,

and

tobacco

plants

while

culturing

Pseudomonas

syringae

on

frequent

basis

•

Prepared

master

mix,

aliquots

and

operated

PCR

machines

while

properly

organizing

all

research

progress

report

•

SELECTED

PRESENTATIONS

1.

FISH,

PCR,

karyotyping

and

cell

morphology

analysis

of

Ph+

cells

for

CML

diagnosis

and

IST-

571

mechanism

of

BCR-

ABL

inhibition,

resistance,

and

available

alternative

treatments

(2011).

2. Diabetes

associated

end

stage

renal

disease

(ESRD)

and

progressive

changes

on

GBM

and

ECM

that

leads

to

a

declining

in

GFR

and

increase

in

proteinuria

(2011).

3. DHHS

administration

of

CLIA

through

approved

laboratory

test

monitoring

agencies

and

state

health

regulatory

agencies

on

new

instrument

procurement,

correlation,

and

method

validation

(2011).

4. Laboratory

Administrative

Council

(LAC)

presentation

on

FDA

proposed

oversight

of

laboratory

developed

tests

(LDTs)

(2011).

5. Advantages

of

invivo

ROS

profiling

in

Dorsophila

transgens

using

OxyR-

GFP

fusion

H2O2

biosensor

(HyPer)

over

non-

biological

ROS

probes,

mechanisms

of

H2O2

sensing,

and

data

acquisition

by

measuring

changes

in

ratiometric

fluorescence

(2009).

PROFESSIONAL

EDUCATION

DIPLOMA

–

MEDICAL

TECHNOLOGY

AND

QA

SAFETY

EDUCATION

The

Washington

Hospital

School

of

Medical

Technology,

Washington,

DC

Clinical

Immunology,

Diagnostic

Microbiology,

Immunohematology

and

Blood

Banking,

Clinical

Chemistry,

Clinical

Parasitology,

Mycology,

Virology,

Diagnostic

Molecular

Biology,

Hematology,

Hemostasis

/

Coagulation,

Urinalysis

and

Cytodiagnostics,

Clinical

Laboratory

Management,

Lean

Six

Sigma.

BACHELOR

OF

SCIENCE

–

BIOCHEMISTRY

College

of

Agriculture

and

Life

Science,

Virginia

Polytechnic

Institute

and

State

University,

Blacksburg,

Virginia

Cell

&

Molecular

Biology,

Genetics,

Biochemistry,

Biophysical

Chemistry,

Biostatistics,

Microbiology,

Analytical

Chemistry,

Bioethics.

ASSOCIATE

OF

SCIENCE

–

BOLOGICAL

SCIENCE,

Cum

Laude

(3.7

GPA)

Northern

Virginia

Community

College

(NVCC),

Science

Department,

Alexandria,

VA

HIGH

SCHOOL

-

GREAT

DISTINCTION

GRADUATE

(4.0

GPA)

Bole

High

School,

Addis

Ababa,

ET

PROFESSIONAL

TRAINING

/

CERTIFICATIONS

MEDICAL

LABORATORY

SCIENCE

/

MEDICAL

TECHNOLOGY

American

Society

for

Clinical

Pathology

(ASCP)

HIPPA

COMPLIANCE,

GOOD

RESEARCH

CONDUCT,

GENERAL

COMPLIANCE

TRAINING

Washington

Hospital

Center

and

Howard

University

MOUS,

MCP,

A

PLUS

(A+)

TRAINING

Real

Network

Systems

IT

Training

School

AWARDS

CERTIFICATE

OF

APPRECIATION

AND

RECOGNITION

FROM

NVCC

“Outstanding

Contribution

and

Dedication

to

the

Student

Ambassador

and

Volunteer

Exchange

Program”

“Successful

Completion

of

the

Student

Leadership

Training

Program”

“Outstanding

Service

to

Tutorial

Services”

PROFESSIONAL

AFFILIATIONS

AMERICAN

SOCIETY

FOR

CLINICAL

PATHOLOGY

-

MEMBER

PHI

THETA

KAPPA

INTERNATIONAL

HONOR

SOCIETY–

MEMBER

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