Medical Training
Resume:
TEFERA
A.
BOKU,
B.Sc.,
MT
(ASCP)
§
C:
*03-
475-
6314
§
****@**.***
MEDICAL
TECHNOLOGIST
“Producing
Accurate
Laboratory
Results
for
Timely
Diagnosis,
Treatment
&
Discharge
Decisions”
ASCP
certified
laboratory
professional
with
two
college
degrees,
offering
five
years
of
progressive
diverse
laboratory
expertise
both
in
high
complexity
clinical
laboratory
and
biomedical
research
for
evidence
based
&
patient
focused
test
results;
Self
motivated
with
strong
understanding
of
diagnostic
laboratory
procedures
(SOPs),
reference
ranges,
and
ability
to
identify
and
document
quality
control
(QC)
issues
and
activities;
Proficient
in
prioritizing
multiple
laboratory
operations;
Poised
professional
demeanor
under
stress,
impeccable
work
ethics,
adaptable
to
new
training
&
technology,
and
productive
in
team
or
independent
work
environment
CORE
LAB
AUTOMATION
AND
TECHNOLOGY
SKILLS
Sysmex
XE
2100
Hematology
Analyzer
• Dimension
Vista
1500
Analyzer
• AutionMax
AX-
4280
Urine
Chem
Analyzer
•
Sysmex
SP-
1000i
Automated
Smear
System
• ADVIA
Centaur
XP
Immunossay
• iQ200
Autom.
Urine
Microscopy
System
•
Stago
STA-
R
Mechanical
Detection
System
• GEM
4000
Blood
Gas
System
• Uricon-
NE
Refractometer
•
PFA-
100
Platelet
Function
System
•
HISTOCOMPATIBILITY
AND
MOLECULAR
TECHNOLOGY
SKILLS
BD
FACSCalibure
• OpenGene
HIV
Seq.
System
• Cobas
4800
•
BD
HLA-
B27
• COBAS
Ampliprep
• ABBOT
m2000sp
•
UNOS
• Qiagen
Rotor-
Gene
3000
• Nuclesens
EasyMAG
•
BD
Multitest
• AMPLICORE
(CT/NG)
• Various
Thermal
Cyclers
•
Roche
LightCycler
• Biorobot
EZ1
•
BLOOD
BANKING
AND
TRANSFUSION
MEDICINE
SKILLS
• ABO/Rh
Typing
• RBC
Phenotyping
• Cord
Blood
• Antibody
Screen
&
ID
• Massive
Trans.
Procedure
• Transfusion
Reaction
Workup
• Crossmatching
• Pediatric
Aliquot
&
Irradiation
• Ortho
ProVue
Technology:
Cerner
Millennium
(LIS),
EasyLink
Data
Management,
EP
Evaluator
(EE9),
Rotor
Gene
300,
Primer
3,
NCBI
database,
60
wpm,
Word,
Excel,
PowerPoint,
Access,
Outlook
Exchange,
Browsing,
Windows,
Mac,
Linux
platforms,
GraphPad
Prism,
UV-
Probe,
Softmax
Pro,
Gaussian,
Molden,
ISIS
Draw
Leadership:
Team
Collaboration,
Conflict
Resolution,
Vision,
Value
&
Goal
Focused
Synergy
PROFESSIONAL
SUMMARY
MEDICAL
TECHNOLOGIST
(ASCP)
/
MOLECULAR
ONCOLOGY
02/2013
–
04/2013
Quest
Diagnostics
–
Chantilly
Hours
:
40
14225
Newbrook
Dr,
Salary
:
$58,000.00
Chantilly,
VA
20151
(703)
802-
6900
Supervisors/
Lab
Managers/
Technical
Director
:
Christina,
Bruce,
Dr.
Quilu
Pan
Managed
an
excellent
balance
of
accuracy
and
speed
in
high
volume
testing
while
flexibly
covering
up
to
12
hour
shift
to
complete
the
task
at
hand.
• Completed
and
recorded
routine
quality
control
checks,
daily
and
weekly
preventive
maintenance
before
processing
patient
specimen.
• Processed
molecular
oncology
samples,
isolated
RNA
from
plasma
using
ABBOT
m2000sp
and
total
nucleic
acid
from
plasma
using
Nuclesens
EasyMAG
extraction
technique
• Completed
RT-
PCR
for
JAK2
V617,
JAK2
Exon
12,
JAK2
Exon
13
and
MPL
mutation
detection
and
analysis
• Extracted
DNA
using
BioRobot
EZ1
from
heparinized
blood
for
JAK2,
MPL,
IGH
B-
cell,
and
TCR
gamma
T-
cell
gene
rearrangement.
• Performed
amplification
reaction
for
B-
RAF,
PAX8/PPAR,
Ret/PTC1,
Ret/PTC3,
JAK2,
MPL,
IGH
B-
cell,
TCR
gamma
T-
cell,
immunoglobulin
VH,
HRAS,
KRAS,
and
NRAS
mutation,
translocation
or
gene
rearrangement
detection
and
analysis.
• complies
with
proficiency
surveys,
continuing
education,
performance
evaluation
and
professional
development
requirements.
TEFERA
A.
BOKU,
B.Sc.,
MT
(ASCP)
PAGE
TWO
MEDICAL
TECHNOLOGIST
HLA
/HISTOCOMPATIBILITY
TECHNOLOGIST
(ASCP)
08/2011
–
10/2011
Georgetown
University
Hospital
Hours
:
40
110
Irving
Street
NW
Salary
:
$51,000.00
Washington,
DC
20010
(202)
444-
2819
Training
Supervisor,
Jing
Guang
;
Lab
Manager:
Sharon
Novak
Excellent
performance
and
diverse
expertise
in
HLA
diagnostic
laboratory
procedures
(SOPs)
in
high
complexity
clinical
laboratory
environment.
• Isolated
class
I
and
class
II
cells
from
peripheral
blood,
lymph
node,
spleen
specimen
and
enumerated
cells
using
hemocytometry.
• Accurately
completed
serologic
HLA
typing
by
CDC
method
with
utmost
attention
to
detail
while
recording
test
results
and
protecting
patient
privacy
at
all
times.
• Performed
flow
cytometric
HLA
crossmatch
testing
on
donor
cells
and
recipient
serum,
analyzed
crossmatch
data,
documented
and
reported
hsitocompatibility
test
results.
• Knowledgeable
in
HLA
antibody
screening,
identification,
titer
as
well
as
SSP
and
SSO
PCR
molecular
HLA
typing
technique.
• Dependable
in
compatibility
analysis,
reviewing,
verifying,
releasing,
and
recording
all
test
results.
• Prevented
testing
error,
troubleshoot
failed
tests,
performed
routine
maintenance,
calibration,
quality
control,
and
function
test
• Attended
HLA
continuing
education
seminars,
completed
mandatory
trainings
and
weekly
lab
meetings.
MEDICAL
TECHNOLOGY
&
QA
SAFETY
EDUCATION
08/2010
–
08/2011
Washington
Hospital
Center
Hours
:
40
110
Irving
Street
NW
Salary
:
$26,000.00
Washington,
DC
20010
Lab
Manager:
Dr.
John
C.
Rees
(301)249-
5924
/
Program
Director:
Amy
M.
McCarty
(202)877-
3346
Consistently
commended
for
outstanding
performance
and
collaboration
in
multifaceted
laboratory
operations
including
the
evaluation
of
new
SOPs
and
daily
laboratory
operations
in
Dc’s
Largest
900
bed
hospital.
Protected
patient
privacy
at
all
times,
adheres
to
safety
resources,
policies,
CLIA,
FDA,
CAP
&
OSHA
regulatory
guidelines,
and
demonstrated
high
level
of
initiative
in
laboratory
process
improvement
through
Lean
Six
Sigma.
• Conducted
hematology,
urinalysis,
coagulation,
&
chemistry
diagnostic
testing
as
a
generalist
and
delivered
timely
and
quality
results
in
routine
&
specialty
lab
tests
from
blood,
serum,
urine,
CSF,
body
fluids,
and
other
specimens.
• Exceeds
established
turnaround
times,
reports
STAT
and
critical
results
according
to
lab
procedures
and
dependable
in
specimen
handling
and
processing,
analysis,
reviewing,
verifying,
releasing,
and
recording
all
test
results.
• Prevents
collection,
clerical,
specimen
processing
and
testing
error
and
troubleshoots
failed
tests;
performs
routine
maintenance,
calibration,
quality
control,
function
test,
method
validation,
and
instrument
correlation
to
prevent
test
result
discrepancy.
• Maintains
appropriate
level
of
lab
supplies
&
reagents
and
labels
testing
kits,
reagents
with
expiration,
open,
and
receipt
dates.
• Professionally
communicates
panic
values
and
flags
with
physicians
and
technical
information
and
laboratory
incidents
with
laboratory
managers
or
workmates
for
smooth
and
efficient
lab
operation.
• Analyzes
broad
screening
automation
tests
for
metabolic
panels,
function
tests,
markers,
lipid
profile,
TDM
&
drug
screening,
vitamins
&
minerals,
hepatitis,
HgA1c,
ABG,
CSF
protein,
and
ketone
studies.
• Performes
macro/microscopic
&
automated
dipstick
urinalysis,
pap
stain
cytodiagnostics,
clinitest,
icto
test,
acetest,
SSA,
hemastix
confirmatory
tests,
crystal,
cast,
cell,
yeast,
and
bacteria
identification
and
quantification.
• Proficient
in
routine
&
specialized
coagulation
tests,
phlebotomy
procedures
and
other
specialized
lab
tests
such
as
electrophoresis,
osmolality,
fibronectin,
and
Vit
D
• Completed
160
hours
of
hematology
bench
on
manual
CBC
and
differential
analysis,
RBC
morphology
&
coloration,
WBC
special
stains,
reticulocyte
count,
phase
platelet
count,
manual
CSF
&
body
fluid
cell
count
and
differential,
malaria
&
crystal
examination,
sickle
solubility
test,
ESR
(Westergren
Tube
Method),
and
manual
HCT.
• Complies
with
proficiency
surveys,
continuing
education,
performance
evaluation
and
professional
development
requirements.
• Performed
DNA
extraction
from
heparinized
peripheral
blood
and
observed
nucleic
acid
amplification
for
detecting
heterozygous
or
homozygous
hereditary
thrombotic
disorder
(FII,
MTHFR,
FV
Lieden
mutation)
by
melting
curve
analysis
• Trained
on
quantization
of
circulating
HCV
and
HIV
viral
load
using
real-
time
PCR
for
monitoring
therapeutic
efficacy
and
HIV
DNA
sequencing
to
detect
mutations
associated
with
resistance
to
antiretroviral
treatment
• Observed
detection
of
Chlamydia
trachomatis
and
Neisseria
gonorrhoeae
infection
from
urine,
endocervical
and
urethral
swabs
by
DNA
amplification
and
colorimetric
technique
using
HRP
labeled
probes.
TEFERA
A.
BOKU,
B.Sc.,
MT
(ASCP)
PAGE
THREE
MEDICAL
TECHNOLOGIST
MOLECULAR
AND
BIOCHEMISTRY
RESEARCH
ASSISTANT
08/2007
–
08/20010
Physiology
and
Biophysics
Dept,
Howard
University
Hours
:
40
520
W
Street
NW
Salary
:
$31,000.00
Washington,
DC
20059
Primary
Investigators
Dr.
Serdia
O.
Mack
(202)806-
9708
/
Dr.
Ayele
Gugssa
(202)806-
6783
PROJECT
ONE:
HYPOTHALAMIC
CONTROL
OF
ENERGY
EXPENDITURE
AND
BREATHING
In
studying
the
ventillatory
and
orexigenic
effect
of
neuropeptide-
Y
(NPY),
model
animals
brain
are
bilaterally
injected
with
NPY-
SAP
or
Saporine
and
physiologic
and
molecular
data
are
compared.
I
contributed
as
a
molecular
research
support
by
measuring
corticotrophin
releasing
hormone
(CrH)
and
thyroid
releasing
hormones
(TrH)
mRNA
level
and
by
comparing
qPCR
data
between
the
experimental
and
control
animal.
• Developed
new
protein,
DNA,
and
RNA
triple
extraction
procedure
from
neuropeptide
Y-
saporine
and
saline
injected
flash
frozen
rat
paraventricular
nucleus
(PVN)
tissue
for
real
time
-
RT
PCR
and
protein
quantitative
studies.
• Utilized
NCBI
gene
sequence
BLAST
search
for
TrH,
CrH,
and
control
gene
with
Primer
3
application
for
custom
primer
design
for
a
two
step
real
time-
RT
PCR
mRNA
study.
• Determined
RNA
extraction
purity
by
260
to
280
absorption
ratio
and
completed
reverse
transcription
using
MMLV
reverse
transcriptase,
DNTPs,
and
oligod
(dT),
random
and
custom
primers
on
Quiagen
kit.
•
Amplified
cDNA
using
FastStart
SYBR
Green
technology
for
semi
quantitative
real
time-
PCR
on
Rotor-
Gene
3000
and
computed
melting
curve,
and
dCT
data
analysis.
• Performed
agarose
gel
electrophoresis
and
densitometric
analysis
for
PCR
product
variation
on
treated
and
control
animal
brain
tissues.
PROJECT
TWO:
AUTONOMIC
CONTROL
SYSTEMS,
AGING
AND
OXIDATIVE
STRESS
Reactive
oxygen
species
(ROS)
is
generated
during
anomalous
oxidative
phosphorylation
process
in
the
electron
transport
chain
(ETC)
and
prolonged
ROS
attack
leads
to
age
related
snowballing
health
disorders.
I
determined
spatial
and
temporal
differential
ROS
generation,
using
genetically
encoded
biosensor
(HyPer),
and
other
selective
ROS
probes.
I
also
compared
and
characterized
antioxidant
enzyme
expression,
behavioral
variation
and
functional
senescence
analysis.
•
Standardized
quantitative
analysis
on
Drosophila
melanogaster
SOD,
CAT,
and
GST
using
microplate,
gel
activity,
western
blot
&
densitometry
to
determine
organismal
redox
temporal
homeostasis.
• Completed
model
animal
behavioral
analysis
including
locomotion,
life
span,
mortality,
fertility,
oxidative
stress
resistance
(paraquat
&
H2O2)
and
caloric
restriction
studies
for
age
related
measurable
functional
senescence.
• Contributed
in
the
quantitative
and
qualitative
determination
of
both
in
vivo
and
in
vitro
cellular
reactive
oxygen
species
(ROS)
using
HyPer
(H2O2
biosensor)
and
other
selective
probes
combined
with
micro-
scale
dissection
and
microcopy.
• Studied
and
discussed
ROS
related
biological
and
molecular
events
such
as
cell
death,
myopathy
and
nerodegeneration
which
manifests
itself
in
mutagenesis,
carcinogenesis,
diabetes,
inflammation,
and
other
age
related
health
disorders.
• Analyzed
data
for
statistical
significance
using
GraphPad
Prism
and
Excel
and
contributed
such
observation
as
a
preliminary
data
in
a
research
proposal
and
other
research
papers
for
publication.
• Presented
progress
reports,
observations,
new
research
tools,
and
related
publications
on
weekly
laboratory
meetings.
MOLECULAR
RESEARCH
ASSISTANT
10/2005
–
12/2006
Plant
Pathology
and
Physiology
Dept.,
Virginia
Tech.
Hours
:
20
551
Latham
Hall
Salary
:
$10,400.00
Blacksburg,
VA
24061
Primary
Investigator:
Dr.
Boris
Vinatzer
(540)231-
2126
PROJECT:
EVOLUTION
OF
PRE-
AGRICULTURAL
PLANT
PATHOGEN
TO
AGRESSIVE
INVADERS
OF
MONOCULTURE
CROPS
I
participated
in
DNA
sequencing
and
molecular
characterization
of
Pseudomonas
syringae
in
the
Plant
Pathology
and
Physiology
Department
in
Virginia
Tech
as
a
molecular
research
assistant.
This
investigation
entails
the
virulent
gene
acquiring
mechanism
of
wide
plant
host
range
infecting
pathogens.
I
performed
amplification
reaction,
DNA
extraction,
gel
electrophoresis,
imaging,
and
other
molecular
techniques
as
well
as
growing
and
inoculation
of
plant
models.
TEFERA
A.
BOKU,
B.Sc.,
MT
(ASCP)
PAGE
FOUR
MEDICAL
TECHNOLOGIST
Inoculate
tobacco,
tomato
and
arabidopsis
plants
with
different
strains
to
study
virulent
gene
acquiring
mechanisms
•
Assisted
in
DNA
sequencing
of
P.
syringae
by
molecular
techniques
by
designing
primer,
PCR,
electrophoresis
&
gel
imaging
•
Maintained
arabidopsis,
tomato,
and
tobacco
plants
while
culturing
Pseudomonas
syringae
on
frequent
basis
•
Prepared
master
mix,
aliquots
and
operated
PCR
machines
while
properly
organizing
all
research
progress
report
•
SELECTED
PRESENTATIONS
1.
FISH,
PCR,
karyotyping
and
cell
morphology
analysis
of
Ph+
cells
for
CML
diagnosis
and
IST-
571
mechanism
of
BCR-
ABL
inhibition,
resistance,
and
available
alternative
treatments
(2011).
2. Diabetes
associated
end
stage
renal
disease
(ESRD)
and
progressive
changes
on
GBM
and
ECM
that
leads
to
a
declining
in
GFR
and
increase
in
proteinuria
(2011).
3. DHHS
administration
of
CLIA
through
approved
laboratory
test
monitoring
agencies
and
state
health
regulatory
agencies
on
new
instrument
procurement,
correlation,
and
method
validation
(2011).
4. Laboratory
Administrative
Council
(LAC)
presentation
on
FDA
proposed
oversight
of
laboratory
developed
tests
(LDTs)
(2011).
5. Advantages
of
invivo
ROS
profiling
in
Dorsophila
transgens
using
OxyR-
GFP
fusion
H2O2
biosensor
(HyPer)
over
non-
biological
ROS
probes,
mechanisms
of
H2O2
sensing,
and
data
acquisition
by
measuring
changes
in
ratiometric
fluorescence
(2009).
PROFESSIONAL
EDUCATION
DIPLOMA
–
MEDICAL
TECHNOLOGY
AND
QA
SAFETY
EDUCATION
The
Washington
Hospital
School
of
Medical
Technology,
Washington,
DC
Clinical
Immunology,
Diagnostic
Microbiology,
Immunohematology
and
Blood
Banking,
Clinical
Chemistry,
Clinical
Parasitology,
Mycology,
Virology,
Diagnostic
Molecular
Biology,
Hematology,
Hemostasis
/
Coagulation,
Urinalysis
and
Cytodiagnostics,
Clinical
Laboratory
Management,
Lean
Six
Sigma.
BACHELOR
OF
SCIENCE
–
BIOCHEMISTRY
College
of
Agriculture
and
Life
Science,
Virginia
Polytechnic
Institute
and
State
University,
Blacksburg,
Virginia
Cell
&
Molecular
Biology,
Genetics,
Biochemistry,
Biophysical
Chemistry,
Biostatistics,
Microbiology,
Analytical
Chemistry,
Bioethics.
ASSOCIATE
OF
SCIENCE
–
BOLOGICAL
SCIENCE,
Cum
Laude
(3.7
GPA)
Northern
Virginia
Community
College
(NVCC),
Science
Department,
Alexandria,
VA
HIGH
SCHOOL
-
GREAT
DISTINCTION
GRADUATE
(4.0
GPA)
Bole
High
School,
Addis
Ababa,
ET
PROFESSIONAL
TRAINING
/
CERTIFICATIONS
MEDICAL
LABORATORY
SCIENCE
/
MEDICAL
TECHNOLOGY
American
Society
for
Clinical
Pathology
(ASCP)
HIPPA
COMPLIANCE,
GOOD
RESEARCH
CONDUCT,
GENERAL
COMPLIANCE
TRAINING
Washington
Hospital
Center
and
Howard
University
MOUS,
MCP,
A
PLUS
(A+)
TRAINING
Real
Network
Systems
IT
Training
School
AWARDS
CERTIFICATE
OF
APPRECIATION
AND
RECOGNITION
FROM
NVCC
“Outstanding
Contribution
and
Dedication
to
the
Student
Ambassador
and
Volunteer
Exchange
Program”
“Successful
Completion
of
the
Student
Leadership
Training
Program”
“Outstanding
Service
to
Tutorial
Services”
PROFESSIONAL
AFFILIATIONS
AMERICAN
SOCIETY
FOR
CLINICAL
PATHOLOGY
-
MEMBER
PHI
THETA
KAPPA
INTERNATIONAL
HONOR
SOCIETY–
MEMBER
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