Engineering Engineer
Resume:
WU, YONGQI
ADD: **** Gainsborough Ct. Apt D, Columbus, OH Cell: +1-614-***-**** Email: ********@*****.***
Objective:
Seeking for an Engineer or Research Scientist Position in Cell/Tissue Culture, Assay Development,
Bioreactor Design, Translational Medicine and Other Biochemical Engineering-related Fields
Education Background
Ph.D. in Chemical and Biomolecular Engineering June, 2014
The Ohio State University, Columbus Department of Chemical and Biomolecular Engineering
Advisor: Jeffrey Chalmers
Overall GPA: 3.74/4.0
B.S. & Chukochen Honors Degree in Bioengineering June, 2010
Zhejiang University, P.R. China Chukochen Honors College & Dept of Chemical Engineering
Core Course GPA: 4.0/4.0 Overall GPA: 3.88/4.0
Chukochen Honors Degree in Entrepreneurial Study June, 2010
Zhejiang University, P.R. China Chukochen Honors College & School of Management
Core Course GPA: 3.8/4.0 Overall GPA: 3.88/4.0
Skills:
Core: Cell/Tissue Culture, Bioreactor Design, Immunochemistry, Drug Target Analysis,
DNA/RNA Labeling and Engineering, High Throughput Screening, Assay Automation
Instrument: Flow Cytometry, Confocol Microscopy, Nuance Microscopy, Real-Time qRT-PCR,
MacsQuant, NanoSight, Nanostring nCounter, Luminex, GC, HPLC
Programming: Matlab, Visual C++, LabVIEW, R, ChemCAD, SuperPro, JMP
Language: Chinese / English, Full Professional Proficiency
Research Experience
Sept. 2010 – Present
Chemical and Biomolecular Engineering, The Ohio State University
Graduate Research Associate Advisor: Jeffrey Chalmers
Cell Culture / Gene Engineering:
Constructed MCF-7 / ES Cells with Survivin and EGFP Sharing the Same Promoter and Apply This MCF-7 Into
High Throughput Screening System for Testing the Effectiveness and Toxicity of Cancer-related Drug
Maintained Tumor / ES / CHO Cell Line Bank and Supplied Transfected / Original Cell line for Entire Lab and
All Collaborating Labs for 4 Years
Cell / Drug Target Characterization: Collaborating with Dr. Lustberg’s Lab, The Ohio State Univ. Med. Center
Assessed γ-H2AX Expression Level by Endonuclear Immunocytochemical Staining for Tumor Cells in Phase I
Dose Escalation Trial of Veliparib
Established a 6-color Fluorescent Immunochemical Staining Methodology Combined with RNA in situ
Hybridization on Human Tumor-related Cells with Confocal and Nuance Multi-spectral Microscopy System
Characterized and Sorted Heterogeneous Cell Populations in Breast Cancer Patient with 9-color Flow Cytometry
Nucleic Acid Analysis:
Comprehensively Analyzed Enriched Cancer-related Cell Population with RT-PCR, Ion Ampliseq Cancer Hotspot
Panel and Nanostring nCounter Analysis System
DNA FISH Test of HER2/CEP7 and EGFR/CEP7 on Breast /Head & Neck Circulating Tumor Cells
Bioreactor Design Collaborating with Upstream R&D, EMD Millipore
Designed Impeller for Expedited and Energy-efficient Mixing in Fed-batch Bioreactor
Improved 3-D Cell Culture Bioreactor with PET Matrix to Simulate Human Breast Tumor in vivo Environment
Collaborating with Dr. Sooryakumar’s Lab, Dept. of Physics, The Ohio State Univ.
Cell Separation:
Developed, Optimized and Commercialized Quadrupole Magnetic Cell Separation System to Negatively Enrich
Circulating Tumor Cells with CD45 Immunomagnetic Labeling
Designed a Single-cell-based Magnetic Cell Separation Chip to Positively Enrich Circulating Tumor Cells and
Encapsulate Cells in Droplets
Sept. 2008 – Aug. 2010
Department of Chemical Engineering, Zhejiang University, China
Undergrad Research Associate Advisor: Lirong Yang
Enzyme Immobilization:
Synthesized and Evaluated Surface-activated Fe3O4 Magnetic Nanoparticles for Arthrobacter sp. Lipase
Immobilization and Recycling
Fermentation / Gene Engineering:
Screened Pseudomonas Highly Expressing R-2-haloacid Dehalogenase and Acquired Related Gene Sequences.
Expressed the R-2-haloacid Dehalogenase Gene in E. coli via Vector Design and Transfection
Major Publications
Wu, Y., Deighan, C. J., Miller, B. L., Balasubramanian, P., Lustberg, M. B., Zborowski, M., Chalmers, J. J. (2013).
Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion
methodology. Methods, 64(2), 169-182.
Wu, Y., Deighan, C. J., Park, K. J., Peter A., Miller, B. L., Kaumaya, P., Foy, K. C., Lustberg, M. B., Chalmers, J.
J. Combined RNA in situ hybridization and fluorescent immunocytochemical analysis on circulating tumor cells
isolated from metastatic cancer patients. In review.
Lustberg, M. B., Balasubramanian, P., Miller B. L., Garcia-Villa, A., Deighan, C. J., Wu Y., Carothers, S., Berger,
M., Ramaswamy, B., Olson, E. M., Wesolowski, R., Layman, R. M., Mrozek, E., Pan, X., Summers, T. A.,
Shapiro, C. L., Chalmers, J. J. Heterogeneous atypical cell populations are present in blood of metastatic breast
cancer patient. Breast Cancer Res. 16(2):R23.
Major Conference Presentations
Wu, Y., Park, K. J., Deighan, C. J., Peter A., Miller, B. L., Lustberg, M. B., Chalmers, J. J. Multi-parameter
Evaluation of Heterogeneous Circulating Tumor Cells: Combined RNA in situ Hybridization, Immunofluorescent
Analysis, and Nanostring mRNA. 2014 OSUCCC ASM.
Wu, Y., Mathsyaraja, H., Miller, B. L., Deighan, C. J., Shapiro, C., Chalmers, J. J., Ostrowski, M., Lustberg, M. B.
Circulating CD115 Positive Leukocytes in Blood Samples From Patients with Breast Cancer. 2013 OSUCCC
ASM.
Chalmers, J. J., Wu, Y., Deighan, C. J., Lustberg, M. B., Peter A., Miller, B. L., Park, K. J. Simultaneous, m-RNA
and/Or micro-RNA, and protein expression on individual, circulating tumor cells. 2013 AIChE Annual Meeting
#341250
Leadership Experience
President of Green Origin Association Sept. 2007 - Sept. 2009
Primary Association for Environmental Education and Protection in China with 600+ Staff Members
Leader of University Alumni Association Sept. 2008 - Sept. 2010
Led 15-people Team for Alumni Fundraising, Souvenir Marketing and Alumni CaféManaging
Honor
First Prize of Excellent Student Scholarship in Zhejiang University
First-grade Student Scholarship in Chukochen Honors College, Zhejiang University
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