Research Associate

VIDYA BOYANAPALLI

Mail: ***C Foster Drive, Willimantic, CT, 06226

Email: ****************@*****.***

Phone: 860-***-****

PROFILE

. Over 3 years of research experience in areas related to

Molecular Biology and Biochemistry

. Adequately trained in good laboratory practices (GLPs)

. Detail-oriented and proven ability to complete projects on

time; excellent oral and written communication skills; ability

to work individually as well as in a team

SKILLS

. Significant experience in maintenance of cell lines (HCT 8, HEK

293, HeLa), scaling up of cells, culturing of cells in various

formats (96 well plate, 24 well plate and cell culture flasks),

aseptic techniques in cell media preparation, cell based assays,

cryopreservation of cell lines, parasite culture, familiar with

384-well assay

. Extensive knowledge of transporters, hepatocytes systems, enzymes

and ADME transporter studies

. Expertise in Polymerase Chain Reaction (PCR), qPCR, RT-PCR, assay

development, primer design, vector and molecular cloning,

extraction and purification of nucleic acids (DNA & RNA), protein

and DNA electrophoresis, transformation, Southern blotting, DNA

sequencing and sequence analysis, recombinant protein expression

in bacteria

. Efficient in Microsoft Office: Word, Excel, Power point, Access,

Visio, Project, GraphPad prism

. Familiar with siRNA transfection, ELISA, flow cytometry, cell

signaling

. Expertise in protein analysis, optical microscopy, multichannel

pipetting, spectrophotometry, nanovue plus spectrophotometer,

phosphorimager, western blotting

. Hands on experience for upstream process development (running

bench scale culture of E.coli ), scale up/down and bioreactor

operation

. Hands on experience with protein activity assays, ion exchange

chromatography, gel filtration chromatography and familiar with

HPLC

. Extensive technical writing experience, SOPs and versatile in

analyzing results

. Significant experience in using Entrez, PubMed, NCBI, Vector NTI

and other web based software for literature search

RESEARCH EXPERIENCE

August 2011- Present

Graduate Research Assistant, University of Connecticut, Storrs, CT

Investigated mutagenicity and cytotoxicity of abasic sites in E. coli

Involved in the cell culture (HEK 293, HeLa), scaling up of cells

(from 150cm2 flask to 500 cm2 flask), culturing of cells in various

formats (24 well plate and cell culture flasks), aseptic techniques

in cell media preparation, cryopreservation

Familiar with siRNA transfections

August 2009- July 2011

Graduate Research Assistant, Stephen F. Austin State University,

Nacogdoches, TX

. Hands on experience in maintenance of cell lines (HCT 8), scaling

up of cells, culturing of cells in various formats (96 well plate,

24 well plate and cell culture flasks), aseptic techniques in cell

media preparation, cell based assays, cryopreservation of cell

lines, parasite culture

Carried out "Characterization of Cryptosporidium parvum fatty acid

binding protein and its purification by affinity chromatography"

Maintained Human ileocecal colorectal adenocarcinoma (HCT-8) cell

lines and performed parasite culture to infect the HCT-8 cells with

Cryptosporidium parvum oocysts.

May 2009- June 2009

Summer Trainee, Biological E Ltd., Hyderabad, India

. Performed quality control tests such as pH, thiomersol estimation

in anti tetanus serum, phenol estimation in anti venom serum as

per established protocols prior to their market release

TEACHING EXPERIENCE

January 2012- Present

Graduate Teaching Assistant, University of Connecticut, Storrs,

Connecticut

. Supervised weekly lab experiments for general chemistry majors;

gave weekly lectures; graded homework and quizzes

EDUCATIONAL BACKGROUND

Master of Science (Graduated in July 2011)

Stephen F. Austin State University, Nacogdoches, Texas

Major: Molecular Biology; GPA: 3.82/4.00

Master of Science, (Expected May 2014)

University of Connecticut, Storrs, Connecticut

Major: Biochemistry; GPA: 3.72/4.00

AWARDS, SYNERGISTIC AND CO-CURRICULAR ACTIVITIES

Acted as Judge for the young inventors (eighth graders) at

Connecticut Invention Convention (April 2013)

First prize for technical talk on "MRI-A Tool For Imaging The Events

Of Brain", ANITS Engineering College, India (May 2008)

Received consolation prize in elocution "Biotech Vision Documents,

Biotech Parks" conducted by BEAGLE, India (January 2009)

Gave several technical talks at local engineering colleges of Andhra

Pradesh, India on varied topics such as cancer, biometrics,

nanomedicine and nanotechnology (September 2005-April 2009)

Member of American Society of Microbiology (January 2010-Present)

Member of Society of Biological Chemists (SBCI), India (August 2006-

April 2009)

Member of BEAGLE, the student association of Biotechnology

Department, ANITS Engineering College, India (September 2005-April

2009)

Member of e-bt club started by Department of Biotechnology, ANITS

Engineering College, India (January 2008-April 2009)

Contact this candidate