Research Associate

PROFILE: Experience working in research industry for 3 years. Highly

skilled in In Vitro Assays, RT-PCR, SDS-PAGE, ELISA and Confocal

Microscopy. An enthusiast for growth and advancement. A team

player with strong organizational skills. Result oriented while

paying attention to details

RESEARCH EXPERIENCE

New York University Langone Medical Center, New York, NY

NYU Cancer Institute

November 2012 - Present

Research Associate

. Manage the laboratory, order supplies and maintain

inventory

. Culture and maintain human cancer cell lines and

spheres from cancer cells, Develop and validate

protocols as required

. Colony forming assay for characterization of cancer

cells and cancer stem cell-enriched cell lines for

radiation and chemotherapy; biochemical assays

. Confocal microscopy for characterization of stem cell

and localization of proliferation markers; Flow

cytometry - stem cell markers, apoptosis and cell cycle

analysis.

. Analyze mice xanograft data for growth curves and

chemotherapy/radiotherapy response; process mice tumor

samples for histology studies

. Carry out high throughput screening for novel

compounds, miRNAs and siRNA

. Assist on recombinant proteins purification/expression

and enzymatic assays; familiar with mass spectrometry

Department of Dermatology

December 2010 - October 2012

Research Technician

. Performed in vitro cell culture and maintained

mammalian and human cell lines, Developed and validated

protocols as required

. Did toxicity tests to determine efficacy of various

compounds against melanoma; cell-based assays

. Performed cell fractionation, protein extraction and/or

separation /Immunoprecipitation assays

. Conducted immunofluorescence/Confocal Microscopy on

adherent cell lines; Transfected mammalian cells with

fluorescent tags and quantitative analysis for protein

expression

. Worked on Western blot/SDS-PAGE for protein expression

and IEF gel electrophoresis/2D SDS-PAGE for protein

separation

. Tested drug efficacy in 3D skin model (Melanoderm and

Epiderm) and carried out Differential centrifugation

St. John's University, Queens, NY

January 2008 - June 2010

Graduate Research Student

Thesis Research: Use of Prolactin to prevent LPS induced pre - term

delivery in pregnant females

. Studied the pharmacology and toxicology of compounds in

in-vivo animal model with the use of pregnant mice;

Tested the effects of compounds on uterine and

placental tissues

. Conducted gel electrophoresis and Western blot on

proteins extracted from tissue samples which included

tissue homogenization and protein extraction

. Performed RNA isolation and DNA quantification for real

time RT-qPCR to study gene expression samples;

Performed ELISA analysis and other immunoassays

. Prepared Immunohistochemistry slides using animal

tissue samples to verify the presence/absence of

inflammatory cells and performed FACS/Flow cytometry on

samples

. Collaborated with colleague(s) in experiments using a

novel anti-inflammatory agent synthesized at St. John's

University, NY

. Relevant coursework: Neuropharmacology and

Cardiovascular pharmacology

INDUSTRY EXPERIENCE

Gujarat Pharmalab Pvt. Ltd., Ahmedabad, India

January 2007 - May 2007

Trainee/Intern

. Worked in Quality Control lab, Quality Assurance,

Production departments

. Conducted analytical tests such as LC-Mass

Spectrometry, HPLC, Affinity Chromatography and

Capillary Electrophoresis

. Revised/reviewed SOPs to maintain GLP and GMP

. Reported deviations, non-conformations, corrective and

preventive actions

Zydus Cadila Healthcare Ltd., Ahmedabad, India

November 2005

Trainee/Intern

. Worked in Formulation, Production and Quality Control

Department(s)

. Performed dosage calculation(s), packaging and

labeling, HPLC and tablet dissolution studies

. Ensured the maintenance of GLP and GMP

. Created a detailed work report for training period

EDUCATION:

St. John's University, Queens, NY

Graduation: June 2010

Master of Science in Pharmaceutical Sciences, Major in Pharmacology

GPA: 3.2/4.0

Rajiv Gandhi University of Health Sciences, India

Graduation: October 2006

Bachelor of Pharmacy, Minor in Clinical Immunology and Pharmacokinetics

Grade: 3.75/4.0

PUBLICATIONS:

. Sundaram S; Ashby CR; Pekson R; Sampat V; Sitapara R;

Mantell L; Chen C-H; Yen H; Abhichandani K; Munnabgi S;

Khadtare N; Stephani RA; Reznik SE. N, N-

Dimethylacetamide (DMA) Prevents Preterm Birth in a

Murine Model by Up-Regulating Interleukin 10 (IL-10).

Am J Pathol. 2013 Aug;183(2):422-30

. Abhichandani K; Shah N; Sundaram S; Chen C-H; Yeh H;

Reznik SE. Prolactin Controls Infection-Associated

Preterm Birth in a Murine. Int J Gynaecol Obstet. 2012

Oct; 119; S263

HONORS:

. Gold medal for academic excellence in 3rd year Bachelor

of Pharmacy program

COMPUTER SKILLS:

. Proficient in GraphPad Prism, SigmaPlot/SigmaStat, LSM

and Zen Digital imaging softwares

. Experience working with Diva, FlowJo, ModFit LT

softwares

. PC and Macintosh literate, efficient in Microsoft

office applications

MEMBERSHIPS

. Member of American Association of Pharmaceutical Scientists (AAPS)

. Member of American Association of the Advancement in Science (AAAS)

REFERENCES: Available upon request

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