Experienced Mid-level Microbiologist/Fermentationist

Floyd L. Inman III

*** **** ******, *******, **, 28376

*************@*****.***,

Home: 910-***-****

Cell: 910-***-****

OBJECTIVE:

To obtain a full-time position within the scope of Applied Microbiology and Fermentation with an emphasis on bioproducts; antimicrobial production and microbial physiology. Special skills/interests include bacteriology, fermentation systems, bioprocessing, medical biodefense, and medical countermeasure development.

SUMMARY OF QUALIFICATIONS:

* Mid-level microbiologist with eight years of laboratory experience in medical and academic research laboratories.

* Five years of teaching/instructional/training experience in microbiology, fermentation technology, biocontrol, antimicrobial production/isolation, countermeasure development, biochemistry, bioprocessing, DNA analysis, biotechnology, and research strategies.

* Five years of research experience in mass producing entomoparasitic nematodes (Heterorhabditis bacteriophora & Steinernema carpocapsae) and entomopathogenic bacteria (Photorhabdus luminescens & Xenorhabdus nematophila).

* Three years experience in mass producing and isolation of antimicrobials produced by entomopathogenic bacteria (P. luminescens & X. nematophila).

* Five years of experience in laboratory management, mentoring, microbiological consulting.

* Demonstrate technical skills in medical, agricultural, industrial, environmental, and applied microbiology with particular interests in fermentation technology, biocontrol agents, molecular biology and biosecurity/biodefense.

* Knowledgeable in fermentation technology, cell/tissue culture, protein analysis, media formulation, quality control, microbial physiology, and preventative maintenance.

* Possess strong knowledge and skills involving microbial kinetics, enzymatic assays/characterization, and techniques in biochemistry, molecular biology, microbiology, and fermentation.

* Successful in producing quality research.

* Proficient in providing a learning and training environment for researchers and students alike.

TECHNIQUES AND SKILLS:

Microbiology and Fermentation:

Bacterial isolation, characterization and identification, aseptic techniques, bacterial enumeration from samples, minimum inhibitory concentrations (MICs), handling of BSL-1, 2 and 3 organisms, water quality and environmental testing, microbial kinetics, substrate utilization studies, oxygen transfer/uptake rates, media formulation and development, up-scale, contamination control, process development and mass production strategies, basic knowledge of cGMP, cGxP and FDA regulations, drug/product screening and production.

Biochemistry and Cell and Molecular Biology:

Protein purification, PAGE separation, protein determination, Western blot, enzymatic assays and characterizations, ELISA, gene and recombinant DNA cloning, expression vector ligations, PCR, RT-PCR, Southern and Northern blots, DNA and RNA isolations, bacterial transformations, electrophoresis, primary culture of bacterial, fungal, algal, plant, and animal cells/tissues/organisms, cell lysis techniques, staining, bacterial genetics, bacterial/nematode symbiosis, insect mortality/toxicity studies.

Other:

Fermentation systems (1.5 L – 30 L), spectrophotometers (UV/vis and standard), thermal cyclers, luminometers, bacterial identification systems, microscopy (brightfield, phase contrast, and scanning electron), DNA analyzers, automated cell counters, chemistry analyzers, shakers, incubators, laboratory management, scientific training and mentoring of undergraduate and foreign personnel, ordering and purchasing equipment and supplies, grant writing and management, writing peer-reviewed articles, networking laboratory equipment, multi-tasking.

RELATED PROFESSIONAL EXPERIENCE:

Sr. Research Associate, Lead Microbiologist & Research Operations Manager (Aug 2009 – present)

Sartorius-stedim Biotechnology Laboratory,Biotechnology Research and Training Center, University of North Carolina at Pembroke, Pembroke, NC

* Train and supervise undergraduate students and visiting scientists in basic laboratory policies and procedures in the areas of: molecular biology; microbiology; fermentation; nematology; and cell/tissue culture.

* Write scientific publications, grants, SOPs and troubleshooting protocols.

* Scientific research involving insect pathogenic, bacterial symbionts of entomoparasitic nematodes (growth kinetics, metabolite and toxin production, molecular, etc).

* Mass produce entomoparasitic nematodes for biological control purposes (Brave-Guard™ beneficial microworms).

* Teaching assistant for bioprocessing, microbiology, DNA analysis and fermentation technology

* Laboratory, research and financial management (scheduling, inventory, research and safety operations, purchasing, etc).

* Manage and coordinate the activities of a research laboratory or research station.

* Acquire, coordinate, and allocate resources among various research projects.

* Coordinate work between multiple research projects with several principal investigators

* Maintain high levels of scientific skill, knowledge and expertise in specific areas of microbiology and fermentation technology.

* Serve as a reliable source for technical and program-related issues.

* Ability to plan at a strategic development plans, organizational structures, and systems to fulfill mission-driven goals.

* Plan, develop and execute research operations and procedures with ability to arrange and assign work to best use research funds.

* Provide oversight of technical programs and administrative activities in a research setting.

* Coordinate and manage facilities, equipment, supplies and related resources with ability to monitor environmental risks and quality control.

* Design and execute biological experiments without supervision performing independent analysis and data reporting.

* Work with a variety of microbes of agricultural and medical significance.

BioWork Laboratory Coordinator (June 2009 – Aug 2009)

Brunswick Community College, Supply, NC

* Prepared laboratory demonstrations and experiments.

* Volunteered tutoring services and additional cGxP training to students.

* Provided lecturing assistance to the course instructor.

Senior Laboratory Technician (Feb 2008 – June 2009)

Brunswick Community College, Supply, NC

* Prepared laboratory experiments for chemistry, biology and biotechnology classes.

* Maintain bacterial and algal collections

* Mass produced algae in a photobioreactor system to study biodiesel production.

* Performed tutoring and training of incoming student technicians.

* Prepared media, reagents and cultures

* Assisted in instruction for biotechnology related classes.

* Cultured mouse B lymphocytes, Chinese hamster ovary cells and hybridomas.

Medical Laboratory Specialist (Nov 2000 – Apr 2003)

U.S. Army Medical Department, Continental US

* Performed laboratory procedures and elementary and advanced testing on biological and environmental specimens to aid in diagnosis, treatment and prevention of disease.

* Received and logged request for laboratory tests along with collecting labels and accessioning specimens.

* Prepared equipment, reagents and supplies for testing.

* Performed routine tests in urinalysis, hematology, clinical chemistry, microbiology, blood bank and serology to include quality control.

* Performed preventative maintenance and troubleshooting on laboratory equipment.

EDUCATION AND TRAINING:

University of Maryland University College Adelphi, MD (August 2011 - December 2014)

M.S. in Biotechnology

Specialization: Biosecurity & Biodefense

The University of North Carolina at Pembroke Pembroke, NC (August 2009 - May 2011)

B.S. in Biology

Concentration: Molecular Biology

Brunswick Community College Supply, NC (January 2008 - May 2009)

A.A.S. in Biotechnology; A.S.; & A.A.

Southeastern Community College Whiteville, NC (January 2005 - February 2007)

No degree awarded

Medical Laboratory Technology

U.S. Army Academy of Health Science Ft. Sam Houston, TX (September 2000 - April 2001)

Military Training

Medical Laboratory Specialist

SOCIETIES/ORGANIZATIONS:

Sigma Xi, The Scientific Research Society, RTP Chapter January 2014 - present

North Carolina Academy of Science

March 2010 - present

North Carolina Branch of the American Society for Microbiology October 2009 – present

PUBLICATIONS:

Belur, P. D., Inman III, F. L., & Holmes, L. D. (2013). Determination of specific oxygen uptake rate of Photorhabdus luminescens during submerged culture in lab scale bioreactor. Biocontrol Science and Technology, 23(12), 1458-1468. doi: 10.1080/09583157.2013.840361

Belur, P. D., Inman III, F.L., & Holmes, L. D. (01 November 2013). Oxygen uptake rate of Heterorhabditis bacteriophora culture in a bioreactor. [Talk]. 13th Workshop of th IOBC Global Working Group on Mass Rearing and Quality Assurance. Begaluru, India. Retrieved from http://www.nbaii.res.in/iobc/presentation/S2-O8.pdf

Bowen, M., Co, D., Inman, F., & Holmes, L. (2012). Microbial kinetics of Photorhabdus luminescens in glucose batch cultures. Explorations: The Journal of Undergraduate Research and Creative Activities for the State of North Carolina, 7, 14-22. Retrievable from http://uncw.edu/csurf/explorations/documents/mattbowen.pdf

Holmes, L. D. & Inman III, F. L. (2010, October). Trehalose as a carbohydrate source for the culture and stability of the phase I variant of Photorhabdus luminescens. Poster presented at the 3rd American Society for Microbiology Conference on Beneficial Microbes, Miami, FL. Abstract retrievable from http://conferences.asm.org/index.php/2012-02-09-21-04-52/2012-02-09-21-03-56/9-past-conferences/98-3rd-asm-conference-on-beneficial-microbes

Holmes, L. & Inman III, F. L. (2011). Symbiotic properties of the entomopathogenic bacterium, Photorhabdus luminescens, and its nematode partner, Heterorhabditis bacteriophora. [Abstract]. Journal of Nematology, 43(1), 60.

Holmes, L. & Inman III, F. L. (2014). Entrepreneurship in Southeastern North Carolina: A partnership that works. Paper presented at the Open 2014 National Meeting of the National Collegiate Inventors and Innovators Alliance (NCIIA), San Jose, CA. Paper retrievable from http://nciia.org/open/wp-content/uploads/2013/10/HOLMES.pdf

Inman III, F. L. & Holmes, L. D. (2010). Mass production of the beneficial nematode, Heterorhabditis bacteriophora, in submerged culture. [Abstract]. Journal of the North Carolina Academy of Science, 126(3), 119.

Inman III, F. L. & Holmes, L. D. (2010). Screening Photorhabdus luminescens for bacteriostatic properties using a modified version of the Kirby-Bauer method. [Abstract]. Journal of the North Carolina Academy of Science, 126(3), 119.

Inman, F. & Holmes, L. D. (2011). Basics of fermentation technology: Bioreactor designs and batch fermentations. In S. Sethi, S. Singh, R. K. Pal, & A. Arora (Eds.), Fermentation Technology with emphasis on Methods and Scale-up Strategies in Fermentation (pp 189-211). New Delhi, India: Division of Post Harvest Technology, Indian Agricultural Research Institution.

Inman, F. & Holmes, L. D. (2011). Basics of fermentation technology: Microbial growth kinetics. In S. Sethi, S. Singh, R. K. Pal, & A. Arora (Eds.), Fermentation Technology with emphasis on Methods and Scale-up Strategies in Fermentation (pp 212-232). New Delhi, India: Division of Post Harvest Technology, Indian Agricultural Research Institution.

Inman, F. & Holmes, L. D. (2011). Basics of fermentation technology: Fermentation media and microbial nutrition. In S. Sethi, S. Singh, R. K. Pal, & A. Arora (Eds.), Fermentation Technology with emphasis on Methods and Scale-up Strategies in Fermentation (pp 233-256). New Delhi, India: Division of Post Harvest Technology, Indian Agricultural Research Institution.

Inman, F. & Holmes, L. D. (2011). Basics of fermentation technology: Downstream processing. In S. Sethi, S. Singh, R. K. Pal, & A. Arora (Eds.), Fermentation Technology with emphasis on Methods and Scale-up Strategies in Fermentation (pp 257-274). New Delhi, India: Division of Post Harvest Technology, Indian Agricultural Research Institution.

Inman, F. & Holmes, L. D. (2011). Basics of fermentation technology: Centrifugation. In S. Sethi, S. Singh, R. K. Pal, & A. Arora (Eds.), Fermentation Technology with emphasis on Methods and Scale-up Strategies in Fermentation (pp 275-285). New Delhi, India: Division of Post Harvest Technology, Indian Agricultural Research Institution.

Inman, F. & Holmes, L. D. (2011). Basics of fermentation technology: Chromatography. In S. Sethi, S. Singh, R. K. Pal, & A. Arora (Eds.), Fermentation Technology with emphasis on Methods and Scale-up Strategies in Fermentation (pp 286-302). New Delhi, India: Division of Post Harvest Technology, Indian Agricultural Research Institution.

Inman III, F. L. & Holmes, L. D. (2012). The effects of trehalose on the bioluminescence and pigmentation of the phase I variant of Photorhabdus luminescens. Journal of Life Sciences, 6, 119-129.

Inman III, F. L. & Holmes, L. (2012). Antibacterial screening of secreted compounds produced by the phase I variant of Photorhabdus luminescens. Indian Journal of Microbiology, 52(4), 708-709. doi:10.1007/s12088-012-0307-6

Inman III, F. L. & Holmes, L. (2012). Effect of heat sterilization on the bioactivity of antibacterial metabolites secreted by Xenorhabdus nematophila. Pakistan Journal of Biological Sciences, 15(20), 997-1000. doi: 10.3933/pjbs.2012.997.1000

Inman III, F. L., Singh, S., & Holmes, L. D. (2012). Mass production of the beneficial nematode Heterorhabditis bacteriophora and its bacterial symbiont Photorhabdus luminescens. Indian Journal of Microbiology, 52(3), 316-324. doi: 10.1007/s12088-012-0270-2

Kooliyottil, R., Upadhyay, D., Inman III, F., Mandjiny, S., Holmes, L. (2013). A comparative analysis of entomoparasitic nematodes Heterorhabditis bacteriophora and Steinernema carpocapsae. Open Journal of Animal Sciences, 3(4), 326-333. doi: 10.4236/ojas.2013.34049

Menefee, M., Holmes, L. D., & Inman III, F. L. (2013, March). Developing entrepreneurship and innovation in rural southeastern North Carolina: An update. Paper presented at the Open 2013 National Meeting of the National Collegiate Inventors and Innovators Allegiance, Washington, D.C. Paper retrievable from http://nciia.org/sites/default/files/features/conference/2013/papers/holmes.pdf

Moreau, E., Inman III, F., Singh, S., Walters, H., & Holmes, L. (2011). Remote control of fed-batch fermentation systems. Journal of Chemistry and Chemical Engineering, 5, 897-902.

Rao, B. M., Inman III, F. L., & Holmes, L. D. (2013). Bioluminescence and chitinase production during chitin fermentation by Vibrio harveyi. Journal of Life Science, 7(5), 491-494.

Rao, B. M., Inman III, F., Holmes, L., & Lalitha, K. V. (2013). Chitinase production in a fed-batch fermentation of colloidal chitin using a mixed culture of Vibrio harveyi and Vibrio alginolyticus. Fishery Technology, 50, 66-74.

Singh, S., Moreau, E., Inman, F., & Holmes, L. D. (2012). Characterization of Photorhabdus luminescens growth for the rearing of the beneficial nematode Heterorhabditis bacteriophora. Indian Journal of Microbiology, 52(3), 325-331. doi: 10.1007/s12088-011-0238-7

Talkington, A., Inman III, F. L., & Holmes, L. D. (2013). A novel method for determining microbial kinetics. Journal of Life Science, 7(8), 787-790.

Talkington, A., Inman III, F., Holmes, L. D., & Wei, G. (2013). An extension of a logistic model for microbial kinetics. Advances in Systems Science and Application, 13(1), 80-99.

Upadhyay, D., Kooliyottil, R., Mandjiny, S., Inman III, F. L., & Holmes, L. D. (2013). Mass production of the beneficial nematode Steinernema carpocapsae utilizing a fed-batch culturing process. Journal of Plant Pathology, 2(1), 52-58.

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