Manager Project

Qualifications Summary

Analytical, dedicated, and detail-oriented PhD scientist, with hands-on

experience in the areas of molecular biology, genetics, and genomics.

Enthusiastic to apply extensive research, critical thinking, and

communication skills as well as competencies in research studies and

scientific projects; research program development; and laboratory

management. Excel at leading, mentoring, and developing cross-functional

teams to enhance their competencies toward fulfillment of corporate target

goals and objectives.

Education

1999-2008 Doctor of Philosophy in Genetics- Microbiology

University of Madras, Chennai, India

1996-1998 Master of Science in Microbiology

Bharathidasan University, Tiruchirappalli, India

1993-1996 Bachelor of Science in Microbiology

University of Madras, Chennai, India

Skills and Expertise

- As a lab manager, involved in the recruitment, training and supervision

of graduate students, postdoctoral fellows, and technicians.

- Maintained active participation in several research projects and

successfully met project deadlines.

- Worked closely with the Principal Investigators and collaborators to

monitor the research grants progress.

- Successful at independently designing and executing scientific projects.

- Prepared NIH progress reports; Identified and resolved scientific and

technical problems to move forward the research projects.

- Organized interdepartmental meetings and seminar series; Assisted in

conducting nationwide workshops and conferences

- Technical Skills: Well versed in next generation DNA and RNA sequencing

and large-scale data analysis; Microarray and IPA analysis; molecular

biology techniques including, RTPCR, qRTPCR, cloning, over-expression of

recombinant proteins, protein purification, Western blot, ELISA, cDNA and

NGS library construction, Northern blot and basic microbiology and

biochemistry techniques; Maintenance of various mammalian cell lines,

siRNA design, lentiviral vector manipulation, generation of stable

knockdown cell lines, cell survival assays, Immunofluorescence, CHIP

assay, Flow cytometry.

- Computer Skills: Microsoft Office Suite (Word, PowerPoint, and Excel);

knowledgeable of Sequencher DNA Sequence Analysis Software, Galaxy,

Intergrated Genome Browser, SeqMonk and Avadis NGS Softwares, IPA

Analysis, GeneSpring, GraphPad, Photoshop, ImageJ, Canvas, and

Statistical Software (R and SPSS). Extensive knowledge in public genomic

data resources of the NCBI, UCSC and ENSEBL.

Professional Experience

University of Toledo, College of Medicine and Life Sciences, Toledo, OH,

USA

Assistant Professor (Research Track) Department of Physiology and

Pharmacology 2011-Present

Play a vital role for National Institutes of Health (NHLBI) funded projects

in the department of physiology and pharmacology with annual cost of $1.2M.

Oversee multiple research projects and set priorities to complete and meet

projected deadlines. Prepare the annual progress report. Gather and

document all preliminary data for a NIH grant application. Conduct research

with several genetically diverse animals, including generation and

characterization of gene knockout and knock in animals, congenic rat

strains for cardiovascular disease, Quantitative Trait Locus (QTL) mapping,

and investigation of regulatory gene expression by microarray and RNA

sequencing and functional analysis.

Postdoctoral Fellow

2008-2011

Compiled preliminary data for a National Heart, Lung, and Blood Institute

(NHLBI) grant application with a projected budget of more than $1.5M

annually. Managed the development of 26 congenic rat strains with a

substitution genetic mapping strategy, monitoring a colony of over 500

rats, resulting in the genotyping of more than 3,000 rats in a year.

Genetic mapping of the region on rat chromosome 1, 9, and 10 involved in

the blood pressure regulation. Identification of the disease causative

genetic factor/gene based on its location on the rat genome by linkage

analysis and Quantitative Trait Locus (QTL) mapping by substitution mapping

and/or gene expression and protein expression profiling using whole genome

systems biology approaches. Performed cell biology and molecular biology

experiments that resulted in high-impact publications.

University of Madras, Department of Genetics, Chennai, India

Guest Faculty and Lecturer Molecular Biology Program

2006-

2007

Provided molecular biology and genetics lectures to students taking up

their master's degree in Molecular Biology. Performed periodical

evaluations of 45 to 50 students and members of junior technical staff.

Project Fellow, University Grants Commission

2001-2003

Held accountability in administering the cloning and immunoscreening of a

cell wall associated protein gene of Mycobacterium tuberculosis to

determine antibodies response in the serum of the majority of the

individuals with tuberculosis infection which indicated the antigen was

capable of stimulating a humoral immune response.

Kanmani Human Fertility Centre, Chennai, India

Junior Embryologist Cytogeneticist

2005-2006

Conducted chromosomal analysis by preparing cultures for peripheral blood,

cell harvesting, and slide preparation.

University of Madras Affiliated College, Tamil Nadu, India

Lecturer Department of Microbiology

1998-1999

Handled and taught 45 graduate students on various subjects, including

general microbiology, biotechnology, and immunology. Made contribution to

laboratory projects, assisted students in a self-contained classroom with

their final project. Developed students' microbiology techniques and

critical thinking skills.

Awards and Honors

New Investigator Award: Annual High Blood Pressure Research Conference

(2011)

American Heart Association Council for High Blood Pressure Research,

Orlando, FL, USA

Special Recognition Awards (2010-2011)

University of Toledo College of Medicine and Life Sciences, Toledo, OH, USA

International Society of Hypertension (ISH) Poster Award (2011)

ISH New Investigators Symposium, Orlando, FL, USA

New Investigator Travel Award: Annual High Blood Pressure Research

Conference (2010)

American Heart Association Council for High Blood Pressure Research,

Washington, DC, USA

Boehringer Ingelheim Young Investigator Travel Award (2010)

Finalist Young Investigator-in-Training Abstract Competition (2010)

American Society of Hypertension, 25th Annual Scientific Meeting, New York,

NY

Selected Presenter - Trainee Highlights in Physiological Genomics Session

(2010)

American Physiological Society at Experimental Biology, Anaheim, CA, USA

Professional Affiliations

2012- Present, American Physiological Society (APS)

2009-Present, American Heart Association (AHA)

2005-Present, International Society of Infectious Diseases (ISID)

2012- 2008, Indian Association of Biomedical Scientists (IABMS)

Journal Review Editorial Board Grant Review I Invitations

2011-Present, American Heart Association (AHA) Grant Peer Review Committee

2011-Present, American Medical Journal (Editorial Board)

2013, Physiological Genomics (Invited for peer Review)

2012, Colloids and surfaces B: Biointerfaces (Invited for peer Review)

2012, Frontiers in Genomic Physiology (Editorial Board)

Publications

Kumarasamy S., Gopalakrishnan K., Mell B., Morgan E., Waghulde H and Joe B.

Cross-talk between two transcription factors, Nr2f2 and Fog2, is

critical for maintenance of normal blood pressure. Nature

Communications, 2014, under revision.

Atanur, SS., Diaz, AG., Maratou, K., Sarkis, A., Rotival

Gopalakrishnan, K and Aitman, TJ. Genome sequencing reveals loci

under artificial selection that underlie disease phenotypes in the

laboratory rat. Cell, 2013, 154(3), 691-703.

Pillai, R., Waghulde, H., Nie, Y., Gopalakrishnan, K., Kumarasamy, S.,

Farms, P. ... and Joe, B. (2013). Isolation and high-throughput

sequencing of two-closely linked epistatic hypertension susceptibility

loci with a panel of bicongenic strains. Physiol Genomics, 2013, 45(16),

729-36.

Kumarasamy, S., Gopalakrishnan, K., Abdul-Majeed, S and Joe. B.

Construction of two novel reciprocal conplastic rat strains and

characterization of cardiac mitochondria, Am J Physiol Heart Circ

Physiol, 2013, 304(1):H22-32.

Gopalakrishnan, K., Kumarasamy, S., Abdul-Majeed, S., Kalinoski, A.,

Morgan, EE, Gohara, AF Joe, B. Targeted disruption of

adamts16 gene in a rat genetic model of hypertension. Proc Natl Acad Sci

U S A, 2012, 109(50), 20555-9. (This article was highlighted in Nat Rev

Nephrol. 2013 Feb; 9(2):64. doi: 10.1038/nrneph.2012.268)

Gopalakrishnan, K., Kumarasamy, S., Yan, Y., Liu, J., Kalinoski, A

Joe, B. Increased expression of rififylin in a <330 kb congenic strain

is linked to impaired endosomal recycling in proximal tubules. Front

Genet, 2012, 3, 138.

Kothandapani, A., Gopalakrishnan, K., Kahali, B., Reisman, D., and Patrick,

SM. (2012). Downregulation of SWI/SNF chromatin remodeling factor

subunits modulate cisplatin cytotoxicity. Exp Cell Res, 2012, 318(16),

1973-86.

*Kumarasamy, S., *Gopalakrishnan, K., Kim, DH., Abraham, NG., Johnson, WD.,

Joe, B, and Gupta, AK. (2011). Dysglycemia induces abnormal circadian

blood pressure variability. Cardiovasc Diabetol, 2011, 10(1), 104 (Co

first authors).

Kumarasamy, S., Gopalakrishnan, K., Toland, EJ., Yerga-Woolwine, S., Farms,

P., Morgan, EE., and Joe, B. (2011). Refined mapping of blood pressure

quantitative trait loci using congenic strains developed from two

genetically hypertensive rat models. Hypertens Res, 2011, 34(12), 1263-

70.

Gopalakrishnan, K., Morgan, E E., Yerga-Woolwine, S., Farms, P.,

Kumarasamy, S., Kalinoski, A., Liu, X., Wu, J., Liu, L., and Joe B.

Augmented rififylin is a risk factor linked to aberrant cardiomyocyte

function, short qt-interval and hypertension. Hypertension, 2011, 57:764-

771.

Gopalakrishnan, K., Kumarasamy, S., Rapp, JP., and Joe, B.. Reply to Letter

to the Editor: Mapping genes for hypertension using experimental models:

a challenging and unanticipated very long journey. Physiol Genomics,

2011, 43, 101-102.

*Gopalakrishnan, K*., *Saikumar, J., Peters, CG., Kumarasamy, S., Farms.,

and Joe, B. Defining a rat blood pressure quantitative trait locus

to a <81.8kb congenic segment: comprehensive sequencing and renal

transcriptome analysis. Physiol Genomics, 2010, 42A, 153-161(*Co first

authors).

*Kumarasamy, S., Gopalakrishnan, K*., Shafton, A., Nixon, J., Thangavel,

J., Farms, P., and Joe, B. Mitochondrial polymorphisms in rat genetic

models of hypertension. Mamm Genome, 2010, 21(5-6), 299-306 (*Co first

authors).

Joe, B., Saad, Y., Lee, N., Frank, B., Achinike, O., Luu, T

Gopalakrishnan, K and Bouchard, C. Positional identification of

variants of adamts16 linked to inherited hypertension. Human Molecular

Genetics, 2009, 18, No. 15, 2825-2838.

Cicila, GT., Morgan, E.E., Lee, SJ., Farms, P., Yerga-Woolwine, S

Gopalakrishnan, K and Joe, B. Epistatic genetic determinants of

blood pressure and mortality in a salt-sensitive hypertension model.

Hypertension, 2009, 53, 725-732.

Anbarasi, K., Kathirvel. G., Vani, G., Jayaraman, G., and Shyamala Devi,

CS. Cigarette smoking induces Hsp70 expression and apoptosis in rat

brain: Modulation by bacoside A. Neuroscience, 2006, 138(4),1127-1135.

Selected published abstracts

Gopalakrishnan, K., Kumarasamy, S., Blair, M., Joe, B. (2013). A novel long

non-coding RNA targeting Rififylin is mechanistically linked to the

regulation of blood pressure, cardiac function and survival in a rat

genetic model of hypertension. Rat Genomics and Models meeting. Cold

Spring Harbor Laboratory. Pg 62.

Gopalakrishnan, K., Kumarasamy, S and Joe, B. (2011). Impaired

endosomal recycling in proximal tubules is mechanistically linked to

proteinuria. Inter-Amer Soc Hypertension (IASH) meeting. Hypertension

58(5), E92-E92

Gopalakrishnan, K., Morgan, EE and Joe, B. (2011). Augmented

rififylin is a risk factor linked to aberrant cardiomyocyte function,

short qt-interval and hypertension. American Heart Association (AHA)

conference. Hypertension, 57, 764-771

Gopalakrishnan, K., Saikumar, J., Peters, CG, Kumarasamy, S., Farms, P.,

Yerga-Woolwine, S., and Joe B. (2010). Defining a rat blood

pressure quantitative trait locus to a <81.8kb congenic segment:

Comprehensive sequencing and renal transcriptome analysis. Experimental

Biology Conference. Physiol Genomics, 42A, 153-161

Gopalakrishnan, K., Kalinoski, A., Morgan, EE., Abdul-Majeed, S., Yerga-

Woolwine, S., Farms, P., Joe, B. (2011). Adamts16 is a novel

genetic determinant of blood pressure: Evidence from a zinc-finger

nuclease mediated knockout rat. American Heart Association (AHA)

conference. Hypertension, 58(5), E149-E150

Gopalakrishnan, K., Kumarasamy, S., Thangavel, J and Joe, B.

(2010). Mapping a genetic determinant of blood pressure to <42.5kb: rffl

and targets of novel mirnas are linked to aberrant rat cardiomyocyte

function and hypertension. American Society for Hypertension (ASH)

conference. Journal of Clinical Hypertension, 12(Suppl.1), OR-39, A17

Gopalakrishnan, K., Saikumar, J., Farms, P., Yerga-Woolwine, S., Edward J.,

Toland, WS., ... Joe, B. (2010). A < 81.8kb rat locus homologous to

human chromosome 2 controls blood pressure. American Society for

Hypertension (ASH) conference. Journal of Clinical Hypertension (JCH),

12(Suppl.1), OR-13, A6

Gopalakrishnan, K., Saikumar, J., Peters, C., Kumarasamy, S., Farms, P.,

Yerga-Woolwine, S., ... and Joe, B. (2010). Defining a < 81.8kb rat

blood pressure locus homologous to human chromosome 2: Comprehensive

single nucleotide polymorphisms, renal transcriptome and microRNA

analyses. AHA conference. Hypertension, 56(5), E60-E60

Gopalakrishnan, K., Thangavel, J., Kumarasamy, S., Yerga-Woolwine, S.,

Farms, P., Morgan, E., ... and Joe, B. (2010). Alleles of a S.LEW

congenic rat spanning 320.6kb further increases the BP of the

hypertensive Dahl S rat: Evidence for Rffl and/or two miRNAs as

potential QTL effectors. Experimental Biology Conference. FASEB Journal,

24(3), 792

Gopalakrishnan, K., Yerga-Woolwine, S., Farms, P., and Joe, B. (2009). A

congenic segment (<61kb) containing a single gene from a normotensive

rat increases hypertension of the Dahl salt-sensitive (S) rat.

Experimental Biology Conference. FASEB J, 23, LB92

Gopalakrishnan, K., Yerga-Woolwine, S., Morgan, E., Farms., P., and Joe, B.

(2009). Detection of polymorphisms in predicted precursor microRNAs:

Implications in a blood pressure quantitative trait locus with alleles

of a normotensive rat increasing hypertension in the Dahl salt-sensitive

(S) rat. AHA Conference. Hypertension, 54(4), E46-E46

References will be provided upon request

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