Assistant Construction

Curriculum Vitae

Yi Li

Postdoctoral Associate, Research Foundation of SUNY

Member of American Society for Microbiology since 2010

** ****** **. *** *, Binghamton, NY 13905

434-***-****, *********@*****.***

Specialties: DNA manipulation Electrophoresis PCR qPCR ELISA MIC Recombinant protein expression

Protein purification FPLC HPLC Mass spectrometry Confocal microscopy

EDUCATION

Ph.D., Cell and Molecular Biology Microbiology Biofilm

Binghamton University, Binghamton NY December 2013

• Thesis research with Dr. Karin Sauer entitled “Regulation of dispersion in response to environmental cues

and its contribution to pathogenicity in Pseudomonas aeruginosa.”

B.S., Biological Sciences and Biotechnology

Sun Yat-sen University, Guangzhou, China June 2008

• Honors thesis research with Dr. Xiaodong Lin entitled “ Effect of recombinant NnLea D34 protein on cell

growth and stress tolerance in Escherichia coli.”

PUBLICATIONS

Li Y, Heine S, Entian M, Sauer K, Frankenberg-Dinkel N. 2013. NO-induced biofilm dispersion in Pseudomonas

aeruginosa is mediated by an MHYT domain-coupled phosphodiesterase. J Bacteriol. 2013 Aug;195(16):3531-42.

Li Y, Petrova O. E., Su S, Lau G. W., Panmanee W, Na R, Hassett D. J., Davies D. G., and Sauer K. BdlA, DipA

and induced dispersion contribute to acute virulence and chronic persistence of Pseudomonas aeruginosa.

(submitted to PLoS Pathogen)

SKILLS

Bacterial culture Restriction digest/vector manipulation

Aseptic techniques Lyophillization

DNA/RNA extraction Ultracentrifugation

in vitro transcription Microbial identification/characterization

Spectrophotometry Water sampling and analysis

ELISA Biofilm (continuous flow and static)

Immunoassay Minimum inhibitory concentration test (MIC 96 Well Assays)

Vector construction Recombinant protein expression

Plasmid extraction Protein purification (Ni-NTA, Co-immunoprecipitation)

Inducible gene expression Fast protein liquid chromatography (FPLC)

Transformation High-performance liquid chromatography (HPLC)

Site-direct mutagenesis Metal oxide affinity chromatography

PCR Full-length cDNA library construction

Real time PCR Mass spectrometry (MALDI-TOF, LC/MS)

RT-PCR GFP expression and detection system

SDS/PAGE Confocal microscopy

Western blotting Virulence model (Arabidopsis thaliana)

Software: Proficient in MatLab, COMSTAT, Internet research, MS Office ( Word, Excel, PowerPoint), Windows and

Mac OS, Data analysis. Experience with EndNote, ImageJ, Photoshop.

Continued…

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Languages: Fluent in English, Cantonese and Mandarin.

EXPERIENCE

Postdoctoral Associate

Research Foundation of SUNY, Binghamton NY February 2014-present

• Conducted projects to study signaling pathway of dispersion and pathogenicity of Pseudomonas aeruginosa,

using biofilms reactors, virulence model, mass spec (LC/MS/MS), qPCR, electrophoresis, DNA

manipulation, biochemical analysis and western blot.

Interdisciplinary Research Team Member

Howard Hughes Medical Institute, Binghamton NY May 2013-December 2013

• Collaborated with Chemistry Department to conduct interdisciplinary research in science and engineering.

• Discovered several electrospun fibers could inhibit or promote biofilm formation by using confocal

microscopy.

• Mentored both biology and chemistry department undergraduate students in i nterdisciplinary projects.

Molecular Biologist/Microbiologist, PhD Student

Binghamton University, Binghamton NY August 2009-December 2013

• Discovered a phosphodiesterase involved in the signaling pathway of dispersion induced by signaling

molecule nitric oxide.

• Tested the pathogenicity of chronic bacterial infections in different growth conditions to develop novel

therapies and compounds for treatment.

• Utilized techniques in molecular biology, genetics, recombinant protein expression and purification,

biochemistry, HPLC, mass spectrometry and confocal microscopy to study protein-protein interaction and

dispersion signaling pathway.

• Presented (Including oral presentation and poster presentation) research findings at three international

research conferences and symposia.

• Trained undergraduate students while conducting short-term projects.

Teaching Assistant

Binghamton University, Binghamton NY August 2009-May 2013

• Instructed 32 junior and senior biology major undergraduate students per semester for 4 semesters in

“General Microbiology Laboratory”.

• Instructed 44 students per semester in “Basic Microbiology”.

• Instructed 75 students per each of 3 semesters in “Intro Cell and Molecular Biology Laboratory”, fully

responsible for laboratory preparation and execution.

Research Assistant

Sun Yat-sen University, Guangzhou, China September 2006-June 2008

• Executed independent research on “Determination of the effect of Nnlea D34 protein on recombinant in E.

coli.” using DNA manipulation, recombinant protein expression and microscopy.

• Conducted project “Environmental factors influence onion mitosis peak.” using microscopy.

• Collaborated closely with graduate students, postdoctoral associates, and faculty.

PRESENTATIONS

Oral Presentation, Li Y, Sauer K. (2013) “Membrane bound cyclase CrdA

is essential for dispersion in Pseudomonas aeruginosa”. American Society for Microbiology, 113 th General Meeting,

Denver, CO (International)

Poster, Li Y, Sauer K. (2012) “ The membrane bound cyclase CrdA is required for

Pseudomonas aeruginosa biofilm dispersion and pathogenicity.” American Society for Microbiology, 6th ASM

Conference on Biofilms, Miami, FL (International)

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