Technician Manager

Deborah E. Geiman

** ****** *****

Glen Rock, PA 17327

Home: *** – 227 – 9721

Work: 410 – 502 – 8237

E-mail: ********@****.***

Professional Qualifications

• Over 15 years of laboratory research experience, progressively building upon each year

of knowledge and expertise with a concentration in microbiology, molecular biology, and

cell biology.

• Develop and implement organizational procedures and policies for TB research center

laboratory and BSL-3 lab comprised of over 40 personnel and 6 Principal Investigators.

Manage and supervise daily operations of TB lab, ensure maintenance and resolution of

problems with equipment, facilities and personnel.

• Disseminate information to personnel in the laboratory and oversee compliance. Train

and supervise personnel in proper BSL-3 practices and handling Mycobacterium

tuberculosis.

• Enforce all biosafety standards, radiation standards, BSL-3 and BSL-2 standard practices.

Write-up SOPs for BSL-2, BSL-3 and ABSL-3 laboratories, guide implementation of the

SOPs and monitor compliance.

• Liaise with many internal and external regulatory agencies, vendors, sales reps, and

maintenance services.

• Design and execute independent research projects. Responsible for the direction of the

research, evaluation/interpretation of experimental results and writing manuscripts.

Requires utilizing a detailed knowledge of research procedures and practices. Critique

abstracts, manuscripts and grant proposals. Assist with development of budgets for new

grant proposals, as well as, justifications of those proposed budgets.

• Manage and supervise the bacterial genetics core and a portion of the mouse model core

activities of an NIAID contract. Includes overseeing the generation, characterization,

handling, and storage of a large collection of M. tuberculosis mutants, writing progress

reports, attending contract meetings, reviewing and presenting data on the progress of

bacterial genetics and mouse model core activities. Developing detailed protocols for in

vivo (in mice) virulence assessment requests and corresponding with the requestor on

progress and data updates of project. Work with Colorado State University and BEI

resources to set up a large biologic material archive. Confirm that appropriate material

transfer agreements are in place prior to delivery outside JHU.

• Train and supervise the day-to-day activities of at least three research

technicians. Assist in the performance reviews of these personnel and in interviewing,

screening and hiring new personnel.

Technical Laboratory Experience

DNA and RNA: genomic and plasmid DNA isolation; cloning and engineering of

recombinant DNA constructs; restriction enzyme mapping; DNA sequencing; primer

extension and S1 nuclease mapping; oligonucleotide construction; general PCR and qRT-

PCR methodology; microarray technology; site-directed mutagenesis and deletion

analysis; radioactive/nonradioactive labeling of DNA; Southerns; mRNA and total RNA

isolation; Northerns; RNase protection assay; in vitro transcription; electrophoresis

mobility shift assay; DNaseI footprinting.

Microbiology/Cell Biology: culturing and manipulation of M. tuberculosis, M.

smegmatis, E. coli at BSL-3 and BSL-2 levels; MIC determination; DNA electroporation,

phage transduction, and transposon mutagenesis of bacteria; construction of knock-out

and knock-in lines; cell wall lipid PDIM analysis; mycobacteria infection of

macrophages; tissue culture; isolation of nuclear extracts, membranes and cytoplasmic

fraction; light and fluorescence microscopy.

Protein and Immunology: in vitro translation of proteins; GST pulldown assay;

immunoprecipitation; Bradford, Lowry and BCA protein determination; bacterial

expression and purification of proteins; Westerns; ELISA.

Animal Model Work: aerosol infection of mice with M. tuberculosis, monitoring

progression of disease state, sacrificing, dissection and culturing of tissues for CFU

analysis; tissue dissection and histopathology preparation.

Human Tissue and Tumor Work: extraction of prostaglandins from colon and

esophageal tissues; preparation of specimens for gas chromatography-mass spectrometric

measurements and analysis of data.

Limited Experience: LC-MS and ION Torrent Personal Genome Machine

and FACS flow.

Computer Skills

• Microsoft Office Professional Suite

• Acrobat, Endnote

• Molecular Biology software tools: Vector NTI, Primer 3, NEBcutter, Webcutter,

GenePixPro

• Statistical software: GraphPad Prism

• Skyline, BioCyc

• Tuberculist, TBDB, PUBMED, NCBI Blast

Work Experience

The Johns Hopkins University School of Medicine 1987 - 2014

Laboratory Manager 2001 - 2014

Senior Research Technician 1997 - 2001

Research Technician II 1992 - 1997

Research Technician 1987 - 1992

Education

Hood College, Frederick, MD 1994 Masters

of Science: Molecular Biology

York College of PA, York, PA

Bachelors of Science: Biology and Psychology

Publications

Parthasarathy G, Lun S, Guo H, Ammerman NC, Geiman DE, Bishai WR. Rv2190c, an

NlpC/P60 family protein, is required for full virulence of Mycobacterium tuberculosis. PLoS

One. 2012 Aug 31; 7(8).

Lee JH, Ammerman NC, Nolan S, Geiman DE, Lun S, Guo H, Bishai WR. Isoniazid resistance

without a loss of fitness in Mycobacterium tuberculosis. Nat Commun. 2012 Mar 20; 3:753

Converse PJ, Eisenach KD, Theus SA, Nuermberger EL, Tyagi S, Ly LH, Geiman DE, Guo H,

Nolan ST, Akar NC, Klinkenberg LG, Gupta R, Lun S, Karakousis PC, Lamichhane G,

McMurray DN, Grosset JH, Bishai WR. The impact of mouse passaging of Mycobacterium

tuberculosis strains prior to virulence testing in the mouse and guinea pig aerosol models. PLoS

One. 2010 Apr 21; 5(4).

Abdul-Majid KB, Ly LH, Converse PJ, Geiman DE, McMurray DN, Bishai WR. Altered

cellular infiltration and cytokine levels during early Mycobacterium tuberculosis sigC mutant

infection are associated with late-stage disease attenuation and milder immunopathology in

mice. BMC Microbiol. 2008; 8:151.

Lee JH, Geiman DE, Bishai WR. Role of stress response sigma factor, SigG, in Mycobacterium

tuberculosis. J Bacteriol. 2008. 190(3): 1128-33.

Geiman, DE, Raghunand, TR., Agarwal, N. & Bishai, WR. Differential Gene Expression in

Response to Exposure to Antimycobacterial Agents and Other Stress Conditions among Seven

Mycobacterium tuberculosis whiB-Like Genes. Antimicrob Agents Chemother. 2006. 50: 2836-

41.

Geiman DE, Kaushal D, Ko C, Tyagi S, Manabe YC, Schroeder BG, Fleischmann RD, Morrison

NE, Converse PJ, Chen P, Bishai WR. Attenuation of late-stage disease in mice infected by the

Mycobacterium tuberculosis mutant lacking the SigF alternate sigma factor and identification of

SigF-dependent genes by microarray analysis. Infect Immun. 2004 Mar; 72(3): 1733-45.

Giardello FM, Casero RA Jr, Hamilton SR, Hylind LM, Trimbath JD, Geiman DE, Judge KR,

Hubbard W, Offerhaus GJ, Yang VW. Prostanoids, ornithine decarboxylase, and polyamines in

primary chemoprevention of familial adenomatous polyposis.

Gastroenterology. 2004 Feb; 126(2): 425-31.

Lamichhane G, Zignol M, Blades NJ, Geiman DE, Dougherty A, Grosset J, Broman KW, Bishai

WR. A postgenomic method for predicting essential genes at subsaturation levels of

mutagenesis: application to Mycobacterium tuberculosis. Proc Natl Acad Sci

U S A. 2003 Jun 10; 100(12): 7213-8.

Dang DT, Zhao W, Mahatan CS, Geiman DE, Yang VW. Opposing effects of Kruppel-like factor

4 (gut-enriched Kruppel-like factor) and Kruppel-like factor 5 (intestinal-enriched Kruppel-like

factor) on the promoter of the Kruppel-like factor 4 gene. Nucleic Acids Res. 2002 Jul 1; 30(13):

2736-41.

Giardiello FM, Yang VW, Hylind LM, Krush AJ, Petersen GM, Trimbath JD, Piantadosi S,

Garrett E, Geiman DE, Hubbard W, Offerhaus GJ, Hamilton SR.

Primary chemoprevention of familial adenomatous polyposis with sulindac.

N Engl J Med. 2002 Apr 4; 346(14): 1054-9.

Chen X, Johns DC, Geiman DE, Marban E, Dang DT, Hamlin G, Sun R, Yang VW.

Kruppel-like factor 4 (gut-enriched Kruppel-like factor) inhibits cell proliferation by blocking

G1/S progression of the cell cycle.

J Biol Chem. 2001 Aug 10; 276(32): 30423-8.

Zhang W, Geiman DE, Shields JM, Dang DT, Mahatan CS, Kaestner KH, Biggs JR, Kraft AS,

Yang VW. The gut-enriched Kruppel-like factor (Kruppel-like factor 4) mediates the

transactivating effect of p53 on the p21WAF1/Cip1 promoter.

J Biol Chem. 2000 Jun 16; 275(24): 18391-8.

Yang VW, Geiman DE, Hubbard WC, Spannhake EW, Hylind LM, Hamilton SR, Giardiello FM.

Tissue prostanoids as biomarkers for chemoprevention of colorectal neoplasia: correlation

between prostanoid synthesis and clinical response in familial adenomatous polyposis.

Prostaglandins Other Lipid Mediat. 2000 Jan; 60(1-3): 83-96.

Geiman DE, Ton-That H, Johnson JM, Yang VW. Transactivation and growth suppression by

the gut-enriched Kruppel-like factor (Kruppel-like factor 4) are dependent on acidic amino acid

residues and protein-protein interaction.

Nucleic Acids Res. 2000 Mar 1; 28(5): 1106-13.

Mahatan CS, Kaestner KH, Geiman DE, Yang VW. Characterization of the structure and

regulation of the murine gene encoding gut-enriched Kruppel-like factor (Kruppel-like factor 4).

Nucleic Acids Res. 1999 Dec 1; 27(23): 4562-9.

Jiang MS, Tang QQ, McLenithan J, Geiman D, Shillinglaw W, Henzel WJ, Lane MD.

Derepression of the C/EBPalpha gene during adipogenesis: identification of AP-2alpha as a

repressor. Proc Natl Acad Sci U S A. 1998 Mar 31; 95(7): 3467-71.

Hwang CS, Mandrup S, MacDougald OA, Geiman DE, Lane MD.

Transcriptional activation of the mouse obese (ob) gene by CCAAT/enhancer binding protein

alpha. Proc Natl Acad Sci U S A. 1996 Jan 23; 93(2): 873-7.

Cheneval D, Christy RJ, Geiman D, Cornelius P, Lane MD. Cell-free transcription directed by

the 422 adipose P2 gene promoter: activation by the CCAAT/enhancer binding protein. Proc

Natl Acad Sci U S A. 1991 Oct 1; 88(19): 8465-9.

Christy RJ, Kaestner KH, Geiman DE, Lane MD. CCAAT/enhancer binding protein gene

promoter: binding of nuclear factors during differentiation of 3T3-L1 preadipocytes. Proc Natl

Acad Sci U S A. 1991 Mar 15; 88(6): 2593-7.

Christy RJ, Yang VW, Ntambi JM, Geiman DE, Landschulz WH, Friedman AD, Nakabeppu Y,

Kelly TJ, Lane MD. Differentiation-induced gene expression in 3T3-L1 preadipocytes:

CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte-

specific genes. Genes Dev. 1989 Sep; 3(9): 1323-35.

Chua CC, Barritault D, Geiman DE, Ladda RL. Induction and suppression of type I collagenase

in cultured human cells. Coll Relat Res. 1987 Sep; 7(4): 277-84.

Chua CC, Geiman DE, Ladda RL. Receptor for epidermal growth factor retains normal

structure and function in aging cells. Mech Ageing Dev. 1986 Mar; 34(1): 5-55.

Chua CC, Geiman DE, Keller GH, Ladda RL. Induction of collagenase secretion in human

fibroblast cultures by growth promoting factors. J Biol Chem. 1985 May 10; 260(9): 5213-6.

Chua CC, Geiman DE, Schreiber AB, Ladda RL. Nonfunctional epidermal growth factor

receptor in cells transformed by Kirsten sarcoma virus. Biochem Biophys Res Commun. 1984

Jan 30; 118(2): 538-47.

Chua CC, Geiman D, Ladda RL. Transforming growth factors released from Kirsten sarcoma

virus transformed cells do not compete for epidermal growth factor membrane receptors. J Cell

Physiol. 1983 Oct; 117(1): 116-22.

Chua CC, Geiman DE, Ladda RL. Detection of an Mr 200,000 glycoprotein in the culture

medium of skin fibroblasts from patients with Huntington disease.

Biochem Biophys Res Commun. 1983 Mar 16; 111(2): 690-9.

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