Project Assistant

Xiaoling Wang Ph.D. Curriculum vitae

CONTACT INFORMATION

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Xiaoling Wang Ph.D. Curriculum vitae

Name: Xiaoling Wang

Address: **** **** ****** ****, ****** CA 91010

Telephone: 415-***-****

Email: ********@***.***

Visa Status: U.S. Permanent Resident.

RESEARCH EXPERIENCE

Postdoctoral Fellow, Department of Virology, Beckman Research Institute at Duarte, CA (2011-present)

Project: Study the role of Mir-9 in Spinal Muscular Atrophy using human pluripotent cells

- Lead a project to assemble gene specific Tale nuclease and CRISPR for genetic modification in

cancer cell lines and human pluripotent cells.

- Conduct unbiased genome-wide analysis of the off-target effects of CRISPRs and TALENs using

Illumina platforms.

- Construct a genetically modified human ES and iPS models to study Mir-9 in motor neuron

differentiation.

- Delineate Mir-9 function in the differentiation of SMA-patient derived iPS cells.

Postdoctoral Fellow, Department of Urology, University of California at San Francisco, CA (2009-2011)

Project: Used small RNA as a tool to activate programming genes for induced pluripotent stem cells

(iPS cells) generation

- Successfully designed a screening assay to identify saRNAs which can increase the reprogramming

efficiency of mouse embryonic fibroblast (MEF) into induced pluripotent stem (iPS) cells.

- Created and optimized a reliable reporter assay to efficiently screen Nanog-inducing saRNAs.

Doctoral Research, Roswell Park Cancer Institute, State University of New York at Buffalo (2002-2008)

Project: Investigated the physiological role of Thoc1 using transgenic mouse models

- Generated Thoc1 conditional knockout mouse model and hypomorphic mouse model.

- Discovered and characterized the essential role of Thoc1 in reproduction and early embryonic

development using multiple internal/external cellular and molecular techniques.

- Identified and validated the downstream target genes of Thoc1 from gene expression profiling data.

Biostar Genechip Inc., Shanghai, Research Assistant (2001-2002)

- Developed a diagnostic kit for alpha-Thalassemia, a severe inherited blood disorder in Asia.

- Designed and optimized probes for detecting three major deletion mutation of α-thalassemia.

- Trained and supervised many entry-level technicians.

BUSINESS EXPERIENCE

Siegal Consulting, Market Analysis Internship (Oct 2013-present)

- Perform market research and competitive landscape analysis for a wide variety of therapeutic

projects.

- Identify and analyze novel technologies through studying literatures, clinical trials, and market

position.

Technology Transfer Office at UC Riverside, Student Marketing Consultant (Oct 2013-Dec 2013)

- Led a three-person team to develop a marketing plan for a novel cancer therapy.

- Performed a comprehensive analysis of competitive landscape, technology feasibility, and market

valuation of the technology.

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Xiaoling Wang Ph.D. Curriculum vitae

EDUCATION

Ph.D. in Pharmacology Roswell Park Cancer Institute, State University of New York at Buffalo, NY. 2002-2008

- Roswell Park Division Award for Dissertation Excellence 2008

- Best Platform Presentation Pharmaceutical Sciences Day 2007

- University Travel Award, State University of New York at Buffalo 2006

Certificate in Bioscience Management, Keck Graduate Institute of Applied Life Sciences, CA 2012-2014

M.S. in Virology Wuhan University Wuhan P.R.China, 1997-2000

- University Award for Dissertation Excellence 2000

B.S. in Virology Wuhan University Wuhan P.R.China, 1993-1997

- Wuhan University student fellowship 1994-1997

MENTORING

• Scientific Mentor: Trained and supervised undergraduate, junior graduate students and technicians

• Teaching assistant: Trained undergraduate students to use molecular biological tools and perform

database research

PUBLICATIONS

Xiaoling Wang, Xinji Chen, Yingjia Wang, Buyuan Chen, Ren-Jang Lin and Jiing-Kuan Yee. Precise gene

modification mediated by TALEN and single-stranded oligodeoxynucleotides in human cells. Plos

one, 2014, Apr. 1st

Xiaoling Wang, Yebo Wang, Xiwei Wu, Jinhui Wang, Zhaojun Qiu, Tammy Chang, Ren-Jang Lin and

Jiing-Kuan Yee. An unbiased approach to study the off-target effect of CRISPR/Cas9 reveals its

tolerance of bulge formation. (Submitted to Nature Biotechnology)

Laura Pitzonka, Xiaoling Wang, Sumana Ullas, David W. Wolff, Yanqing Wang, and David W. Goodrich.

The THO ribonucleoprotein complex is required 1 for stem cell homeostasis in the mouse small

intestine. Mol Cell Biol, 2013. 33(17):3505-3514.

Xiaoling wang, Ji Wang, Rob Place, Vera Huang, and Long-Cheng Li. Induction of NANOG expression by

targeting promoter sequence with small activating RNA antagonizes retinoic acid-induced

differentiation. Biochemical Journal, 2012, 443:821–828

Robert F Place, Ji Wang, Emily J Noonan, Rachel Meyers, Muthiah Manoharan, Klaus Charisse, Rick Duncan,

Vera Huang, Xiaoling Wang and Long-Cheng Li Formulation of Small Activating RNA Into Lipidoid

Nanoparticles Inhibits Xenograft Prostate Tumor Growth by Inducing p21 Expression. Molecular Therapy

Nucleic Acids (2012) 1, e15; doi:10.1038/mtna.2012.5

Vera Huang, Yi Qin, Ji Wang, Xiaoling wang, Rob Place, and Long-Cheng Li. RNAa is conserved in

mammalian cells. Plos One, 2010, 5(1): e8848

Ji Wang, Rob Place, Vera Huang, Xiaoling wang, Emily J. Noonan, Clara E. Magyar, Jiaoti Huang and

Long-Cheng Li. Prognostic Value and Function of KLF4 in Prostate Cancer: RNAa and Vector-

Mediated Overexpression Identify KLF4 as an Inhibitor of Tumor Cell Growth and Migration .

Cancer Res, 2010 70; 10182

Xiaoling Wang, Meena Chinnam, Xiaojing Zhang, Yanqing Wang and David W. Goodrich . Thoc1

deficiency compromises gene expression necessary for normal testis development in the mouse.

Mol Cell Biol, 2009. 29(10): 2794-803.

Xiaoling Wang, Yanping Li, Xiaojing Zhang, and David W. Goodrich. An allelic series for studying the mouse

Thoc1 gene. Genesis, 2007. 44: 32-37.

Yanping Li, Athena W. Lin, Xiaojing Zhang, Yanqing Wang, Xiaoling Wang, and David W. Goodrich. Cancer

Cells and Normal Cells Differ in TheirRequirements for Thoc1. Cancer Res, 2007. 67(14): 6657-64.

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Xiaoling Wang Ph.D. Curriculum vitae

Yanping Li, Athena W. Lin, Xiaojing Zhang, Yanqing Wang, Xiaoling Wang, and David W. Goodrich. Thoc1 as a

potential molecular target for cancer therapy. Medicina-Buenos Aires, 2007 67:13-19.

Xiaoling Wang, Yanjie Chang, Yanping Li, Xiaojing Zhang, and David W. Goodrich. Thoc1/Hpr1/p84 is essential

for early embryonic development 1 in the mouse. Mol Cell Biol, 2006. 26(11): 4362-4367

Yangping Li, Xiaoling Wang, Xiaojing Zhang, and David W. Goodrich. Human hHpr1/p84/Thoc1 regulates

transcriptional elongation and physically links RNA polymerase II and RNA processing factors. Mol Cell Biol,

2005. 25(10): 4023-33.

Ming Li, Peixin Cai, Liping Nie, Minghui Huang, Xiaomei Tao, Yuzhu Li, Xiaoling Wang, Zhu Xiang, Mingchun

Wang and Mengsu Yang. Detection of thalassemia using ThalaChip. Chinese Journal of Hematology, 2003

24(10): 551-552.

Shayan Wang, Peixin Cai, Ruanzhang Zhang, Fangfang Li, Xiaoling Wang, Yong Dai and Mengsu Yang.

Application of microarray in screening deletion mutation of α-thalassemia. Chinese Journal of Hematology, 2002

23(11): 603-604.Xiaoling Wang, Linbai, Ye, Jingrong Gao, Jinping Xu, Hui Zeng. Expression of a human TNF-α

mutant in E.coli and measurement of its ability to induce apoptosis. Wuhan University Journal of Natural

Sciences, 2001 Vol.47 No.2 P.200-204.

MEETING ABSTRACTS

Xiaoling Wang, Yinjia Wang, Buyuan Chen, Xinji Chen, Tammy Chang, Ren-Jang Lin and Jiing-kuan Yee.

TALEN and single-stranded oligodeoxynucleotide-mediated gene editing in human tumor cell lines and

embryonic stem cells. CSH annual meeting, 2013

Xiaoling Wang, Yanping Li, Yanqing Wang, Xiaojing Zhang and David W. Goodrich. Thoc1 is involved in breast

cancer tumorigenesis. AACR annual meeting, 2008.

Xiaoling Wang, David W. Goodrich. Thoc1 functions in mouse development, reprodu Buyuan Chen, Xinji

Chen, Tammy Chang, Ren-Jang Lin and Jiing-kuan Yee

ction and tumorigenesis. AACR annual meeting, 2007.

Xiaoling Wang, David W. Goodrich. The role of Thoc1 in mouse development, reproduction and tumorigenesis.

14th Annual Graduate Student Association Poster Competition, 2007

Xiaoling Wang, Yanping Li, Yanjie Chang, Xiaojing Zhang, and David W. Goodrich. Thoc1 is required for early

embryonic development in the mouse. AACR annual meeting, 2006.

SKILLS

• Cell Biology: Human and mouse ES cell culture, tissue culture, transfection,TALEN and CRISPR mediated

genetic modification, generating human and mouse iPS cells, murine xenograft models and teratoma

formation, fluorescence microscopy, reporter assay, flow cytometry, motor neuron differentiation.

• Molecular Biology: Gene cloning, quantitative Real Time PCR, southern blotting, lentiviral and retrovirual

vector design, construction and virus production; virus tittering and purification mutagenesis, nucleic acid

extraction, purification and quantification, cloning, western blotting, Chromatin immunoprocipitation (ChIP),

total RNA microarray, next generation sequencing and data analysis.

• Animal Modeling: Mouse genome modification, in situ immuno-staining, tissue microarray, breeding and

genotyping, anesthesia, necropsy, histology.

• Computer skills: Word; Powerpoint; Excel; PRISM, basic bioinformatics analysis, R statistics etc.

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