Experienced Biochemist

Lei Jiang, PhD

**** * **** **. ***. B**, Lawrence, KS 66046

469-***-****, ************@*****.***, Permanent Resident

PROFESSIONAL STATEMENT

A biochemist seeking a R&D position at a pharmaceutical or biotechnology company

Thirteen-year experience in Neuroscience research

Extensive expertise in developing and troubleshooting analytical assays

Long-term career goal: to identify biomarkers to help diagnosis of diseases; to study disease pathological

mechanisms to facilitate development of therapeutics

WORK EXPERIENCE

University of Kansas, School of Pharmacy, Higuchi Biosciences Center Sep 2004 – Present

Senior Research Associate, Research Associate, Postdoctoral Research Associate

Research

- Established mechanisms of brain aging and cognitive decline in neurodegenerative diseases;

summary of research work resulting in 1 review article (in preparation)

- Identified the plasma membrane Ca2+-ATPase (PMCA) as well as a range of synaptic membrane

lipid raft proteins and lipids as potential brain aging biomarkers, resulting in 2 publications

- Isolated the PMCA enzyme and characterized protein distribution, expression, activity and

regulation in rat brain synapses and primary neuronal cultures, resulting in 2 publications

- Core lab member contributing to the $6.4MM grant renewal from the National Institute on Aging

Key Lab Skills

- Developed analytical procedures and protocols including ELISA, immunoblotting, co-

immunoprecipitation, immunochemistry, enzyme activity assay, two-dimensional fluorescence

difference gel electrophoresis, mass spectrometry, and high performance thin layer

chromatography

- Became proficient in the cell culture system operation and rodent handling

- Set up and maintained a proteomics lab

Lab Management

- Supervised a research team of two Research Assistants and one undergraduate student

- Collaborated with Mass Spec and Bioinformatics core laboratories for lipidomic and proteomic

characterization

Graduate Research Experience Jul 2000 – Jun 2004

Shanghai Jiao Tong University, China, Proteomics Laboratory Manager Nov 2002 – Jun 2004

Research

- Screened protein biomarkers for schizophrenia in the body fluids of Chinese patient population,

resulting in 4 publications

Key Lab Skills

- Set up a proteomics lab by transferring Proteomics Technology learned from Roche during

exchange program, developed lab protocols and standard operating procedures

- Qualified instruments and tested the automation of the spot handling workstation and mass

spectrometer

- Evaluated the affinity chromatography protocols

Lab Management

- Supervised a research team of one postdoc, one Research Assistant, and two PhD students

F. Hoffmann La Roche Ltd., Switzerland, Exchange Scholar Aug 2001 – Oct 2002

Research

- Identified protein biomarkers of schizophrenia in the human CSF and plasma samples, resulting in

1 publication

- Developed protocols of affinity and hydrophobic interaction chromatography to enrich low-

abundance proteins in plasma to facilitate the biomarker discovery, resulting in 2 publications

Key Lab Skills

- Two-dimensional gel electrophoresis, image analysis, gel spot picking, gel digestion

- Protein fingerprinting identification via MALDI-TOF, peptide sequencing via LTQ mass spectrometer,

affinity and hydrophobic interaction chromatography

Chinese Academy of Science, Shanghai Institute of Physiology, China, Jul 2000 – Aug 2001

Research Assistant

Research

- Studied on DNA biomarkers of schizophrenia in 20 schizophrenia candidate genes

Key Lab Skills

- DNA sequencing, SNPs mapping, qPCR, RNA isolation and reverse transcription

Chinese Academy of Science, Shanghai Institute of Biochemistry, China, Feb 1999 – Jun 1999

Undergraduate Intern Student

- Expressed and purified the prokaryotic protein of recombinant Histidine-tagged Hepatitis C viral

envelope protein

EDUCATION

1999 – 2004 Ph.D. degree in Biochemistry and Molecular Biology, Shanghai Jiao Tong University,

Shanghai, China and F. Hoffmann La Roche Ltd., Basel, Switzerland

Dissertation: Screening of Schizophrenia-Associated Proteins on a Large-Scale by Using

Proteomics Technology

1995 – 1999 B.S. degree in Biotechnology, Shanghai Jiao Tong University, Shanghai, China

PROFESSIONAL MEMBERSHIP/AWARDS

Member of SfN, AAPS, HUPO, and The Proteome Society

Manuscript reviews for Journal of Nutritional Biochemistry and Alcoholism: Clinical and Experimental

Research

Honors at 21st School of Pharmacy Graduate Honors Symposium, 2006

Siemens graduate fellowship, Shanghai Jiao Tong University, 2000

Jiao Tong University student scholarship, 1997 and 1998

Second place in National University Student Literary and Artistic Performance, piano accompanist in violin

ensemble, 1996

PUBLICATIONS

Jiang L (2014). Brain aging and Ca2+ dysregulation (invited review). Advances in Aging Research. (In

preparation)

Jiang L (corresponding author), Bechtel MD, Bean JL, Winefield R, Williams TD, Zaidi A, Michaelis EK,

Michaelis ML (2014). Effects of gangliosides on the activity of the plasma membrane Ca2+-ATPase. BBA

Biomembranes 1838(5): 1255-1265.

Jiang L, Bechtel MD, Galeva NA, Williams TD, Michaelis EK, Michaelis ML (2012). Decreases in plasma

membrane Ca -ATPase in brain synaptic membrane rafts from aged rats. J Neurochem 123(5): 689-99.

Jiang L, Fang J, Moore DS, Gogichaeva NV, Galeva NA, Michaelis ML, Zaidi A (2010). Age-associated

changes in synaptic lipid raft proteins revealed by two-dimensional fluorescence difference gel

electrophoresis. Neurobiol Aging 31(12): 2146-59.

Zhang M, Wan C, Ji B, Zhang Z, Zhu H, Tian N, La Y, Huang K, Jiang L, He G, Gao L, Zhao X, Shi Y,

Huang G, Feng G, He L (2009). Proteome alteration of U251 human astrocytoma cell after inhibiting

retinoic acid synthesis. Mol Cell Biochem 323(1-2): 185-93.

Jiang L, Fernandes D, Mehta N, Bean JL, Michaelis ML and Zaidi A (2007). Partitioning of the plasma

membrane Ca2+-ATPase into lipid rafts in primary neurons: Effects of cholesterol depletion. J Neurochem

102(2): 378-88.

Wan C, La Y, Zhu H, Yang Y, Jiang L, Chen Y, Feng G, Li H, Sang H, Hao X, Zhang G, and He L (2007).

Abnormal changes of plasma acute phase proteins in schizophrenia and the relation between

schizophrenia and haptoglobin (Hp) gene. Amino Acids 32(1): 101-8.

Wan C, Yang Y, Li H, La Y, Zhu H, Jiang L, Chen Y, Feng G, and He L (2006). Dysregulation of retinoid

transporters expression in body fluids of schizophrenia patients. J Proteome Res 5(11): 3213-6.

Yang Y, Wan C, Li H, Zhu H, La Y, Xi Z, Chen Y, Jiang L, Feng G, and He L (2006). Altered levels of acute

phase proteins in the plasma of patients with schizophrenia. Anal Chem 78(11): 3571-6.

Fountoulakis M, Juranville JF, Jiang L, Avila D, Roder D, Jakob P, Berndt P, Evers S, and Langen H (2004).

Depletion of the high-abundance plasma proteins. Amino Acids 27(3-4): 249-59.

Jiang L, He L and Fountoulakis M (2004). Comparison of protein precipitation methods for sample

preparation prior to proteomic analysis. J Chromatogr A 1023(2): 317-20.

Jiang L, Lindpaintner K, Li HF, Gu NF, Langen H, He L and Fountoulakis M (2003). Proteomic analysis of

the cerebrospinal fluid of patients with schizophrenia. Amino Acids 25(1): 49-57.

PRESENTATIONS

Poster presenter at 28th School of Pharmacy Graduate Student Honors

Jan 16, 2013

Symposium

Dec 3, 2010 Poster presenter at Higuchi Biosciences Center Science Talks

Poster presenter at Society for Neuroscience 2010, the 40th annual meeting,

Nov 13-17, 2010

San Diego, CA, USA

Poster presenter at the 41st annual meeting of American Society for

Mar 6-10, 2010

Neurochemistry, Santa Fe, NM, USA

Dec 5, 2008 Poster presenter at Higuchi Biosciences Center Science Talks

Poster presenter at Society for Neuroscience 2008, the 38th annual meeting,

Nov 15-19, 2008

Washington DC, USA

Nov 28, 2007 Poster presenter at Higuchi Biosciences Center Science Talks

Apr 20, 2007 Lecture presenter at Department Graduate Symposium

Apr 21, 2006 Lecture presenter at Department Graduate Symposium

Lecture presenter at 21st School of Pharmacy Graduate Honors Symposium

Jan 17, 2006

PERSONAL INFORMATION

Fluent in English and Chinese

Permanent Resident (Green Card Holder)

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