Medical Development
Resume:
Yiguo Shen, Ph.D
***** ****** **, ****** ****** CA 94546
Cell Phone: 510-
Email:
*****.****@*****.***
CAREER OBJECTIVES
A Research & Development position in which molecule biology, cell
biology, biochemistry, and in vivo animal model applied towards new
medicine for human diseases.
PROFILE HIGHLIGHT
. Experienced veteran in molecular & cell biology, biochemistry and
genetics, human disease studies including transcription regulation,
protein expression & interaction, primary cell cultures,
inflammation and metabolite assays, in vivo viral delivery, animal
model and behaviours. Hands-on experience in lentivirus and
adenovirus constructs and in vivo gene delivery, conditional
knockout construction, stable siRNA knockdown cell lines etc.
. Superb communication skills, self motivated team player.
. Design, implement, analyze, and present experiments to demonstrate
feasibility and achieve project goals. Solve complex research and
development challenges independently and quickly.
EDUCATION
Ph.D. Genetics, Institute of Genetics, Chinese Academy of
Sciences, China, 2002.
B.S. Biology, University of Science and Technology of
China, China, 1997.
METROLOGY AND SKILLS
. DNA&RNA Techniques, Molecular Cloning, Library Construction, Virus
Construction, Conditional
Knockout Construction, Real-Time PCR, Confocal, Electron
Microscopy;
. Mammalian cell culture, Expression System, Protein Purification and
Interaction, ELISA, Enzymes activity Assay, Cell-based and
metabolites Assays;
. Mouse Genetics, Mapping and Mutation Cloning, Mouse Behaviour
Tests, Zero-maze, Open Field, Forced Swim, Stereotaxic Virus
Injection;
. Immunohistochemistry, Confocal, Transmission Electron Microscopy.
SELECTED SCHOLASTIC HONORS
. 2009 Bachmann Strauss Dystonia & Parkinson Foundation Grant
Award
. 2007 Dystonia Medical Research Foundation Fellowship
PROFESSIONAL EXPERIENCE
July, 2013 - Present: Senior Scientist, Manager of Bioassay Development,
Nvigen Inc., Santa Clara CA
. Develop novel method to quantify nanoparticle binding sensitivity,
capacity. Develop new reagents and protocols for non-hybridization
and hybridization based DNA-binding nanoparticles. Optimize assay
condition and improve nanoparticle recovery efficiency. Develop new
method for Antibody-based Cell Capture Assay in blood analysis.
. Provide technique support to various customers, and co-supervise
the product and catalogue development.
July, 2012 - June, 2013: Assistant Adjunct Professor, Veteran Affairs
Medical Center, San Francisco CA
Northern California Institute for Research&Education
. Discovered the molecular mechanism of the metabolism regulated
inflammatory responses through a C-terminal binding protein in the
microglia and macrophage. Revealed the role of metabolic NAD+/NADH
sensor protein in regulating NF-?B activated inflammatory pathway.
Wrote a manuscript titled "Bioenergetic State Regulates the
Inflammatory Response through NADH sensor CtBP", co-authored two
other publications.
July, 2010 -June, 2012: Associate Specialist, Veteran
Affairs Medical Center, San Francisco CA
Northern
California Institute for Research&Education
. Established an in vitro model of metabolism regulated inflammation
in macrophage and microglia, identified CtBP as the metabolism
sensor regulating pro-inflammatory genes transcription.
. Characterized the first PNKD mouse model and dissected the genetic
interaction between PNKD and RIMS genes by behaviour studies and
immunohistology.
. Collaborated with Dr. Wooping Ge in the Jan Lab at UCSF on
electrophysiology and identified short-term plasticity changes in
the PNKD knockout mouse.
. Wrote a manuscript "A protein mutated in paroxysmal dyskinesia
suppresses synaptic vesicle exocytosis through the active zone
protein RIM" (Submitted to Neuron), and co-authored one
publication.
. Independently conceived and wrote half of an NIH R01 grant
proposal. The proposal was reviewed and revised by the time I left.
July, 2007 -June, 2010: DMRF Fellow, Dept. of Neurology, University
of California, San Francisco CA
. Identified the inhibitory role of PNKD in neurotransmitter release
through its association with pre-synaptic protein RIMS; illustrated
this through protein interaction, isolation and Mass Spectrometry,
synaptic component isolation, immune-gold Transmission Electronic
Microscopy, TIRF, etc.
. Collaborated with Dr. Yulong Li in Richard Tsien Lab and Dr. Pascal
Kaeser in Thomas Sudhof lab at Stanford University on synaptic-
physiology using real-time imaging of synaptic release.
. Wrote a manuscript "Mutations in PNKD Causing Paroxysmal
Dyskinesia Alters Protein Cleavage and Stability" (Human Molecular
Genetics 2011).
. Independently conceived and wrote a grant proposal for Bachmann
Strauss Dystonia & Parkinson Foundation. The proposal was approved
and funded for one year.
March, 2002 -June, 2007: Postdoctoral Fellow, Dept. of Neurology,
University of California, San Francisco CA
Howard Hughes Medical Institute
. Identified a point-mutation that causes cleavage fault and
subsequent protein stability fault in the human dyskinesia disease
gene PNKD.
. Analyzed the enzymatic activity of PNKD and collaborated with Dr.
Sunil Ojha in Patricia Babbitt lab at UCSF to simulate the
substrate binding motif and to screen possible compounds docking
through KEGG.
. Identified and characterized the transcription bi-regulator
activity of Atrophin proteins.
. Mouse forebrain development mutants screening and map-based cloning
of Megalin nonsense point mutation.
. Delivered 2 first-author (Journal of Biological Chemistry, Cellular
Molecular Life Sciences) and 2 co-author publications to help
secure research funding.
. Independently conceived and wrote a fellowship proposal for
Dystonia Medical Research Foundation. Proposal was approved and
funded for two years.
SELECTED PUBLICATIONS
1. Yiguo Shen, Angela Brennan, Yong Huang, Raymond Swanson. Bioenergetic
State Regulates the Inflammatory Response through NADH sensor CtBP
(Manuscript in Preparation).
2. Yiguo Shen, Wooping Ge, Yulong Li, Pascal Kaeser, Hsien-Yang Lee,
Richard Tsien, Thomas C. Sudhof, Lily Jan, Ying-Hui Fu, Louis J. Ptacek.
A protein mutated in paroxysmal dyskinesia suppresses synaptic vesicle
exocytosis through the active zone protein RIM (Submitted).
3. Tina Lam, Angela Brennan-Minnella, Seok Joon Won, Yiguo Shen, Colleen
Hefner, Yejie Shi, Dandan Sun, Raymond Swanson. Intracellular pH
reduction prevents excitotoxic and ischemic neuronal death by inhibiting
NADPH oxidase (In Revision).
4. Angela Brennan, Yiguo Shen, Raymond Swanson. Phosphoinositide 3-kinase
couples NMDA receptor activation to superoxide release and propagation
of excitotoxic injury. Cell Death and Disease. 2013, 4: e580.
5. Reno Reyes, Angela Brennan, Yiguo Shen, Ylva Baldwin, Raymond Swanson.
Activation of Neuronal NMDA Receptors Induces Superoxide-Mediated
Oxidative Stress in Neighboring Neurons and Astrocytes. Journal of
Neuroscience. 2012, 32:129**-*****.
6. Hsien-yang Lee, Junko Nakayama, Ying Xu, Xueliang Fan, Maha Karouani,
Yiguo Shen, Emmanuel N. Pothos, Ellen J. Hess, Ying-Hui Fu, Robert H.
Edwards and Louis J. Ptacek. Dopamine dysregulation in a mouse model of
paroxysmal nonkinesigenic dyskinesia. Journal of Clinical Investigation.
2012, 122:507-518.
7. Yiguo Shen, Hsien-Yang Lee, Joel Rawson, Sunil Ojha, Patricia Babbitt,
Ying-Hui Fu, and Louis J. Ptacek. Mutations in PNKD Causing Paroxysmal
Dyskinesia Alters Protein Cleavage and Stability. Human Molecular
Genetics. 2011, 20:2322-2332.
8. Yiguo Shen, Andrew S. Peterson. Atrophins, emerging roles in development
and neurodegenerative disease. Cellular and Molecular Life Sciences.
2009, 66:437 - 446.
9. Yiguo Shen, Gena Lee, Youngshik Choe, J. Susie Zoltewicz, Andrew S.
Peterson. Functional architecture of atrophins. Journal of Biological
Chemistry. 2007, 282: 5037-5044.
10. Scott R. May, Amir M. Ashique, Mattias Karlen, Baolin Wang, Yiguo Shen,
Konstantinos Zarbalis, Jeremy Reiter, Johan Ericson, Andrew S. Peterson.
Loss of the retrograde motor for IFT disrupts localization of Smo to
cilia and prevents the expression of both activator and repressor
functions of Gli. Developmental Biology. 2005, 287:378-389.
11. Konstantinos Zarbalis, Scott R. May, Yiguo Shen, Marc Ekker, John L. R.
Rubenstein, Andrew S. Peterson. A Focused and Efficient Genetic
Screening Strategy in the Mouse: Identification of Mutations That
Disrupt Cortical Development. PLoS Biology 2004, 2:1177-1187.
REFERENCES:
Dr. Andrew Peterson
Director, Department of Molecular Biology, Genentech
Tel: 650-***-****
Email: ********.******@****.***
Dr. Ziyin Li
Assistant Professor, Department of Microbiology and Molecular genetics,
Texas Medical Center
Tel: 281-***-****
Email: *****.**@***.***.***
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