Project Development

Ishita Shah

***** ****** **, ***#**, Fremont, CA-94536

Cell: 714-***-****, Email:****.****.*****@*****.***

Career Objective

To obtain a position in the Research and Development team of Biotechnology or Pharmaceutical company where

I can effectively use my expertise in cell culture and molecular biology techniques, my strong organizational skills

and ability to work in a team

Summary of Skills

Cell culture techniques (Bacterial and Mammalian) Molecular Biology techniques

Mammalian cell culture techniques (RAW, HEK Isolation of DNA (plasmid and genomic) and

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CHO cell lines) RNA and its purification

Polymerase Chain Reaction (PCR) cDNA synthesis

Stable and transient transfections of cells ●

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and quantitative real time PCR

ShRNA mediated gene knockdown

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Agarose and polyacrylamide gel electrophoresis

Primary cell culture (Bone marrow derived ●

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(SDS-PAGE)

macrophages)

Expression vector designing

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Recombinant protein expression

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Gene cloning: Restriction digestion, ligation and

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Baculovirus expression in SF9 insect cells

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transformation of E.coli cells

Protein purification

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Primer Designing, Site directed mutagenesis

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Immunocytochemistry and Immunohistochemistry

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W estern blotting

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Summary of Qualifications

MS Biology

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More than 3 years laboratory experience in cell culture and molecular biology techniques

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Experience in statistical analysis of the data using Excel and Graphpad Prism

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Effective interpersonal skills and communication skills

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Experience

Research Associate - University of California, San Diego July 2010-November 2013

Projects:

Study of Arachidonic acid metabolism and Cox pathway in RAW cells using stable mpges1 (enzyme for

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PGE2) knockdown and ptgds (enzyme for PGD2) knockdown cells. Stable knockdown cells were obtained

using lentiviral based shRNA constructs. To study the effect of knockdowns on the pathway, gene expression

analysis was done using real-time qPCR and western blotting was done to check protein l evels. The levels of

metabolites were analyzed from the media samples using LC-MS.

Study of effect of nicotine on Arachidonic acid metabolism and Cox pathway in RAW and U937 pathway

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Expression of calcium independent phospholipase A2 (iPLA2) in SF9 cell s using baculovirus expression

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system and purification of iPLA2 protein by Affinity Column Chromatography

Screening phospholipase A2 inhibitors using C14 labeled radioactive assay and generating IC50 values for

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potent enzyme inhibitors

Equipment Usage

Experience of using various instruments like HPLC, Mass spectrometer, DNA thermal cycler, spectrophotometer,

nanodrop, sequencer (Genetic analyzer), light microscopes and florescent microsco pe

Education

California State University, Northridge 2011

Northridge, California, USA

Biology

Master of Science

Sardar Patel University 2008

V V Nagar, Gujarat, India

Biotechnology

Master of Science

Sardar Patel University 2006

V V nagar, Gujarat, India

Biotechnology

Bachelor of Science

Thesis/Dissertation

Project 1: Production of Sialic acid affected by GNE gene mutations

Summary:

-Subcloning of mutated GNE gene into pUMVC3 vector (PCR amplification, restriction digestion, ligation)

-Sequencing of GNE gene

-Transfection in CHO cells

-Biochemical Assay for Sialic acid production in CHO cells

Project 2: Isolation and characterization of pentose metabolizing yeast

Summary:

-Isolation of yeast from insect gut and fruits -

biochemical tests for yeast

-cloning and sequencing of 26s rDNA for identification of yeast

Publications

-Targeted Proteomics of the Eicosanoid Biosynthetic Pathway Completes an Integrated Genomics -Proteomics-

Metabolomics Picture of Cellular Metabolism

Eduard Sabid, Oswald Quehenberger,Qin Shen, Veavi Chang, Ishita Shah, Aaron M Armando,

Alex Andreyev,Olga Vitek, Edward A. Dennis, Ruedi Aebersold

-New potent and selective polyfluoroalkyl ketone inhibitors of GVIA calcium independent phospholipase A2

Victoria Magrioti, Aikaterini Nikolaou, Annetta Smyrniotou, Ishita Shah, Violetta Constantinou-Kokotou,

Edward A. Dennis and George Kokotos

Posters

-Development and functional analysis of wild type and R266Q GNE expression plasmids

Y. VallesAyoub, S.L. Martenez, A. Haghighatgoo, I. Shah, C. Saechao, Z. Khokher, S. Esfandiarifard, C.J. Riley, M.

Pietruszka, D. Darvish, at American Society of Gene and Cell Therapy (ASGT) annual meeting.

-Analysis of expression of wildtype and mutated GNE genes

I.Shah, Y. Valles-Ayoub, Z.khoker, D.No, C.Saechao, Z.khoker, S. Metzenberg, D.Darvish A. Metzenberg, at

California State University Program for Education and Research in Biotechnology (CSUPERB) symposium, and

CSUN 14th Annual Symposium * * - First prize winner

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