Engineering University

Lee, Baeck-Seung

Curriculum Vitae

Baeck-Seung Lee, Ph.D.

**** ******** ****** #**

St. Louis, MO 63105

Phone; 314-***-****

Work; 314-***-****

Email: ****@*********.*****.***

Education

Post-doc., Immunology/Pathology, Washington University in St Louis, 2008 - current.

Ph.D., Molecular Biology, University of Texas at Austin, 2007

Dissertation: "Functional Characterization of the B-cell Lymphoma/Leukemia 11A

(BCL11A) Transcription Factor"

M.S., Genetic Engineering, Korea University, Seoul, Korea, 1997

Dissertation: "Cloning and Sequencing of cDNA Encoding Antibacterial peptides from a

Korean Frog"

B.S., Genetic Engineering, Korea University, Seoul, Korea, 1995

Teaching Experience

Microbiology lab (UT Austin)

General Biology Discussion lecture (UT Austin)

Biochemistry lab (Korea University)

Mentoring several Undergraduate students

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Lee, Baeck-Seung

Research Skills

DNA and RNA

; Gene expression or Silencing using plasmid, retrovirus, lentivirus and siRNA, RT-PCR,

qPCR, in vitro transcription/translation, EMSA, northern blot and southern blot, RNase

protection assay, and Microarray, cDNA library construction, DNA sequencing, DNA

fragmentation assays for cell death, In vitro Ttranscription/Translation, Chromatin

immunoprecipitation (ChIP), Inducible protein expressions using Shield, Tet, or Hormone

receptors, Sanger sequencing, Gibson Assembly for gene synthesis.

Protein

; Western blot, whole cell labeling and kinase assay, Co-immunoprecipitation, Tandem

affinity purification for Mass-spec, Expression of GST, His, MBP fusion proteins and

isolation in E.coli, Rabbit antibody production, Monoclonal antibody purification from a

hybridoma culture.

Cell

; Basic cell culture, a small bioreactor and 3D cell cultures, Transfections and

Transductions of DNA or Virus, Inhibitor treatments of ATM, DNA-PKcs, Abl kinases,

and parp1 for gene expression, cell death and DNA repair, In vitro cell differentiation of

B and T cells using cytokines and co-culture.

Others

; Gene editing, Genomic DNA engineering, or gene targeting using Zinc finger nuclease

and Crispr/cas9, luciferase assay, cell isolation and in vitro culture from mouse and pig,

Light Microscopy and Fluorescent Microscopy for detection of proteins, FACS analysis,

cell sorting using Aria or Magnetic beads, Luciferase assay, small animal handlings,

Recombinant influenza virus production, Development of Artificial Liver Unit,

Ultracentrifugation for RNA preparation or virus concentration.

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Lee, Baeck-Seung

Awards

High honor, 1st Semester, 1995

Department of Genetic Engineering. Korea University (Seoul Korea)

High honor, 2nd Semester, 1995

Department of Genetic Engineering. Korea University (Seoul Korea)

Education Assistant Scholarship, 2nd Semester, 1996

Department of Biotechnology. Korea University (Seoul Korea)

National Funds Scholarship, 1st Semester, 1997

Department of Biotechnology. Korea University (Seoul Korea)

National Funds Scholarship, 2nd Semester, 1997

Department of Biotechnology. Korea University (Seoul Korea)

Publications

1. Liu H, Ippolito GC, Wall JK, Niu T, Probst L, Lee BS, Pulford K, Banham AH, Stockwin L,

Shaffer AL, Staudt LM, Das C, Dyer MJ, Tucker PW. Functional studies of BCL11A:

characterization of the conserved BCL11A-XL splice variant and its interaction with

BCL6 in nuclear paraspeckles of germinal center B cells. Mol Cancer. 2006 May 16;5;18.

2. Helmink BA, Bredemeyer AL, Lee BS, Huang CY, Sharma GG, Walker LM, Bednarski JJ,

Lee WL, Pandita TK, Bassing CH, Sleckman BP. MRN complex function in the repair of

chromosomal Rag-mediated DNA double-strand breaks. J Exp. Med. 2009 Mar

16;206;669.

3. Yin B, Lee BS, Yang-Iott KS, Sleckman BP, Bassing CH. Redundant and nonredundant

functions of ATM and H2AX in αβ T-lineage lymphocytes. J Immunol. 2012 Aug 1:189:1372.

4. Jeremy A. Daniel, Manuela Pellegrini, Baeck-Seung Lee, Zhi Guo, Darius Filsuf, Natalya V.

Belkina, Zhongsheng You, Tanya T. Paull, Barry P. Sleckman, Lionel Feigenbaum and

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Lee, Baeck-Seung

André Nussenzweig. Loss of ATM kinase activity leads to embryonic lethality in mice. J

Cell Biol. 2012 Aug6;198:295.

5. Gapud EJ*, Lee BS*, Mahowald GK, Bassing CH, Sleckman BP (*co-first author). Repair

of chromosomal RAG-mediated DNA breaks by mutant RAG proteins lacking

phosphatidylinositol 3-like kinase consensus phosphorylation sites. J Immunol. 2011 Aug

15;187:1826.

6. Natalie C. Steinel*, Baeck-Seung Lee*, Anthony T. Tubbs, Jeffrey J. Bednarski, Emily

Schulte, Katherine S. Yang-Iott, David G. Schatz, Barry P. Sleckman, and Craig H. Bassing

(*co-first author). The Ataxia Telangiectasia Mutated Kinase Controls Igκ Allelic

Exclusion by Inhibiting Secondary Vκ-to-Jκ Rearrangement. J Exp Med. Feb 2013.

7. Baeck-Seung Lee, Joseph D Dekker, Bum-Kyu Lee, Vishwanath R Iyer, Barry Sleckman,

Arthur L. Shaffer, Gregory C. Ippolito, and Philip W. Tucker. The BCL11A transcription

factor directly activates RAG gene expression and V(D)J recombination. Molecular and

Cellular Biology. May 2013.

8. Um JH, Brown AL, Singh SK, Chen Y, Gucek M, Lee BS, Luckey MA, Kim MK, Park JH,

Sleckman BP, Gellert M, Chung JH. Metabolic sensor AMPK directly phosphorylates RAG1

protein and regulates V(D)J recombination. Proc Natl Acad Sci. June 2013 .

9. Baeck-Seung Lee, Eric J. Gapud, Shichuan Zhang, Yair Dorsett, Andrea Bredemeyer, Rosmy

George, Elsa Callen, Jeremy A. Daniel, Oleg Osipovich, Eugene M. Oltz, Craig H. Bassing,

Andere Nussenzweig, Susan Lees-Miller, Michal Hammel. Benjamin P.C. Chen and Barry P.

Sleckman. Functional Intersection of ATM and DNA-PKcs Kinase Activities During

V(D)J Recombination. Molecular and Cellular Biology. July 2013.

10. Gregory C. Ippolito, Joseph D. Dekker, Yui-Hsi Wang, Bum-Kyu Lee, Arthur L. Shaffer III,

Jian Lin, Jason K. Wall, Baeck-Seung Lee, Louis M. Staudt, Yong-Jun Liu, Vishwanath R.

Iyer, and Haley O. Tucker. Dendritic cell fate is determined by BCL11A. Proc Natl Acad

Sci. Mar 2014.

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Lee, Baeck-Seung

References

Dr. Barry Sleckman (Post-doc supervisor)

Washington University in St Louis

Immunology/Pathology

Phone: 314-***-****

E-mail: ********@*****.***

Dr. Eugene Oltz

Washington University in St Louis

Immunology/Pathology

Phone: 314-***-****

E-mail: *****@*********.*****.***

Dr. Philip Tucker (Ph.D. supervisor)

University of Texas at Austin

Molecular Genetics and Microbiology

Phone: 512-***-****

E-mail: **********@****.******.*** (or ***********@******.******.***)

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