Manager Technician

Deborah E. Geiman

** ****** *****

Glen Rock, PA 17327

Home: *** – 227 – 9721

Work: 410 – 502 – 8237

E-mail: ********@****.***

Professional Qualifications

• Over 8 years of microbiology laboratory research experience, progressively building

upon each year of knowledge and expertise.

• Develop and implement organizational procedures and policies for TB research center

laboratory and BSL-3 lab comprised of over 40 personnel and 6 Principal Investigators.

Manage and supervise daily operations of TB lab, ensure maintenance and resolution of

problems with equipment, facilities and personnel.

• Disseminate information to personnel in the laboratory and oversee compliance. Train

and supervise personnel in proper BSL-3 practices and handling Mycobacterium

tuberculosis.

• Enforce all biosafety standards, radiation standards, BSL-3 and BSL-2 standard practices.

Write-up SOPs for BSL-2, BSL-3 and ABSL-3 laboratories, guide implementation of the

SOPs and monitor compliance.

• Liaise with many internal and external regulatory agencies, vendors, sales reps, and

maintenance services.

• Design and execute microbiology research projects in M. tuberculosis. Responsible for

the direction of the research, evaluation/interpretation of experimental results and writing

manuscripts. Requires utilizing a detailed knowledge of research procedures and

practices. Critique abstracts, manuscripts and grant proposals. Assist with development

of budgets for new grant proposals, as well as, justifications of those proposed budgets.

• Manage and supervise the M. tuberculosis bacterial genetics core and a portion of the

mouse model core activities of an NIAID contract. Includes overseeing the generation,

characterization, handling, and storage of a large collection of M. tuberculosis mutants,

writing progress reports, attending contract meetings, reviewing and presenting data on

the progress of bacterial genetics and mouse model core activities. Develop detailed

protocols for in vivo (in mice) virulence assessment requests and corresponding with the

requestor on progress and data updates of project. Work with Colorado State University

and BEI resources to set up a large biologic material archive. Confirm that appropriate

material transfer agreements are in place prior to delivery outside JHU.

• Train and supervise the day-to-day activities of four research

technicians. Assist in the performance reviews of these personnel and in interviewing,

screening and hiring new personnel.

Technical Laboratory Experience

Microbiology/Cell Biology: culturing and manipulation of M. tuberculosis, M.

smegmatis, E. coli at BSL-3 and BSL-2 levels; CFU and MIC determination; DNA

electroporation, phage transduction, and transposon mutagenesis of bacteria; construction

of knock-out and knock-in lines; cell wall lipid PDIM analysis; mycobacteria infection of

macrophages; tissue culture; isolation of nuclear extracts, membranes and cytoplasmic

fraction; light and fluorescence microscopy.

DNA and RNA: genomic and plasmid DNA isolation; cloning and engineering of

recombinant DNA constructs; restriction enzyme mapping; DNA sequencing; primer

extension and S1 nuclease mapping; oligonucleotide construction; general PCR and qRT-

PCR methodology; microarray technology; site-directed mutagenesis and deletion

analysis; radioactive/nonradioactive labeling of DNA; Southerns; mRNA and total RNA

isolation; Northerns; RNase protection assay; in vitro transcription; electrophoresis

mobility shift assay; DNaseI footprinting.

Protein and Immunology: in vitro translation of proteins; GST pulldown assay;

immunoprecipitation; Bradford, Lowry and BCA protein determination; bacterial

expression and purification of proteins; Westerns; ELISA.

Animal Model Work: aerosol infection of mice with M. tuberculosis, monitoring

progression of disease state, sacrificing, dissection and culturing of tissues for CFU

analysis; tissue dissection and histopathology preparation.

Human Tissue and Tumor Work: extraction of prostaglandins from colon and

esophageal tissues; preparation of specimens for gas chromatography-mass spectrometric

measurements and analysis of data.

Computer Skills

• Microsoft Office Professional Suite

• Acrobat, Endnote

• Molecular Biology software tools: Vector NTI, Primer 3, NEBcutter, Webcutter,

• Statistical software: GraphPad Prism

• Tuberculist, TBDB, PUBMED, NCBI Blast

Work Experience

The Johns Hopkins University School of Medicine 1992 - 2014

Laboratory Manager 2001 - 2014

Senior Research Technician 1997 - 2001

Research Technician II 1992 - 1997

Education

Hood College, Frederick, MD

Masters of Science: Molecular Biology

York College of PA, York, PA

Bachelors of Science: Biology and Psychology

Publications

Parthasarathy G, Lun S, Guo H, Ammerman NC, Geiman DE, Bishai WR. Rv2190c, an

NlpC/P60 family protein, is required for full virulence of Mycobacterium tuberculosis. PLoS

One. 2012 Aug 31; 7(8).

Lee JH, Ammerman NC, Nolan S, Geiman DE, Lun S, Guo H, Bishai WR. Isoniazid resistance

without a loss of fitness in Mycobacterium tuberculosis. Nat Commun. 2012 Mar 20; 3:753

Converse PJ, Eisenach KD, Theus SA, Nuermberger EL, Tyagi S, Ly LH, Geiman DE, Guo H,

Nolan ST, Akar NC, Klinkenberg LG, Gupta R, Lun S, Karakousis PC, Lamichhane G,

McMurray DN, Grosset JH, Bishai WR. The impact of mouse passaging of Mycobacterium

tuberculosis strains prior to virulence testing in the mouse and guinea pig aerosol models. PLoS

One. 2010 Apr 21; 5(4).

Abdul-Majid KB, Ly LH, Converse PJ, Geiman DE, McMurray DN, Bishai WR. Altered

cellular infiltration and cytokine levels during early Mycobacterium tuberculosis sigC mutant

infection are associated with late-stage disease attenuation and milder immunopathology in mice.

BMC Microbiol. 2008; 8:151.

Lee JH, Geiman DE, Bishai WR. Role of stress response sigma factor, SigG, in Mycobacterium

tuberculosis. J Bacteriol. 2008. 190(3): 1128-33.

Geiman, DE, Raghunand, TR., Agarwal, N. & Bishai, WR. Differential Gene Expression in

Response to Exposure to Antimycobacterial Agents and Other Stress Conditions among Seven

Mycobacterium tuberculosis whiB-Like Genes. Antimicrob Agents Chemother. 2006. 50: 2836-

41.

Geiman DE, Kaushal D, Ko C, Tyagi S, Manabe YC, Schroeder BG, Fleischmann RD, Morrison

NE, Converse PJ, Chen P, Bishai WR. Attenuation of late-stage disease in mice infected by the

Mycobacterium tuberculosis mutant lacking the SigF alternate sigma factor and identification of

SigF-dependent genes by microarray analysis. Infect Immun. 2004 Mar; 72(3): 1733-45.

Lamichhane G, Zignol M, Blades NJ, Geiman DE, Dougherty A, Grosset J, Broman KW, Bishai

WR. A postgenomic method for predicting essential genes at subsaturation levels of

mutagenesis: application to Mycobacterium tuberculosis. Proc Natl Acad Sci

U S A. 2003 Jun 10; 100(12): 7213-8.

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