Curriculum vitae

Bhupesh Parise Ph.D

D.O.B: **-05-1983

Nationality: Indian

Phone: +49-176********

E-mail: acdc8z@r.postjobfree.com

Academic distinctions:

(Oct - Feb)

**** - 2014 Ph.D, Faculties of Veterinary Medicine and Medicine, Justus Liebig University,

Giessen.

2008-2009 Research assistant at Laboratory for Molecular Ophthalmology, Justus

Liebig University Giessen, Universitätsklinikum Giessen and Marburg GmbH, Giessen

Campus.

2005-2007 Masters in Biotechnology (M.Sc) from Bharathidasan University

2001-2004 Bachelors degree B. Sc (Tripple major-Biotechnology, Botany and

Chemistry) from Kakatiya University

Other degrees

Post Graduate Diploma in Chemical Analysis and Quality management (PGDCAQM)

from Hyderabad Central University, India.

Diploma in Nanotechnology

Statistical Analysis Systems (SAS)

Research Experience:

During my research career I have been involved in various research projects which include:

August 2003- March 2004: (8 Months)

Involved in Department of Biotechnology (DBT), funded project “Tissue culture studies in

tomato (Lycopersicum esculentum)” from Government of India to Kakatiya University where I

got expertise in various plant tissue culture techniques.

October 2006- March 2007: (6 Months)

Masters thesis in Shantha Biotechniques (SANOFI) titled “Cloning of the 5’ portion of 342 bp

of the Alcohol Oxidase gene of Shantha4 in E. coli”. My tasks included construction and

analysis of recombinant molecules, where I got expertise in various molecular biology

techniques.

October 2007- December 2007: (2 Months)

Trained in CHANDRA LABS, Hyderabad for Analytical Testing: FT-IR Spectroscopy, Gas

Chromatography (G.C) and High Pressure Liquid Chromatography (HPLC).

July 2008 – September 2009: (1 Year 3 months)

Worked as Research assistant at the Laboratory for Molecular Ophthalmology, Justus Liebig

University Giessen. My tasks included analysis of LCA genes specially Cloning and recombinant

protein expression studies.

October 2009 – Febraury 2014: (4 years 5 months)

Since October 2009 working as a PhD student under the supervision of Dr. Markus Preising, my

PhD thesis “Expression patterns of AIPL1 Splice variants in relation to Centromere protein

F” deals with functional studies of genetic disorders related to the retina. The main focus of

project is identifying novel interaction partners of Aryl hydrocarbon interacting protein like 1

(AIPL1) and the role of interaction in LCA (Lebers Congenital Amaurosis) which falls under

the cluster of EOSRD (Early Onset of Severe Retinal Dystrophy). Practical aspects of my project

include Molecular Cloning, heterologous expression, Animal cell culture, protein purification

assays and interaction studies.

Animal experience:

Experience in handling animal models Mouse & Rat.

Perfusion studies, isolation of Brain and Eyes from Mouse & Rat.

Isolation of Retina and immunohistochemical studies.

Computer skills:

Familiar with the following softwares: Vector NTI, MERLIN, pDRAW32, GENtle, CLC Main

Workbench 6, BioEdit, Ball view, Chromas Lite, ClustalW, SAS, End notex, Citavi, Unicorn,

Image J, SigmaPlot and GraphPad Prism.

Achievements:

Got selected twice for DAAD Scholarship and support program (STIBET) teaching

assistantship program 2010 and 2011.

Other Experience:

Worked as Organizing committee member for the International Giessen Graduate School

for the Life Sciences (GGL) conferences 2009 and 2010 Giessen, Germany.

Worked as Organizing committee member for EPOS 2010 36th Annual Meeting of the

European Paediatric Ophthalmological Society, New Challenges in Paediatric

Ophthalmology meeting held in Bad Nauheim, Germany.

Ran an institute Phoenix coaching center to train bachelor students for their competitive

exams. My role includes teaching Molecular biology, Biochemistry and organizing

activities of the institute.

Volunteered an institute Transcend educational consultancy to provide guidance for

bachelor students from rural areas in India in opting there PG courses offered in

different Universities in India and abroad.

Membership: ARVO (Association for Research in Vision and Ophthalmology)

Membership: IBS The International Biometric Society ID: 1189116.

Took part in lab rotation High Resolution Molecular Analysis at Kerckhoff Clinic:

Centre for Heart, Lung and Rheumatic Diseases, Bad Nauheim, Germany.

Trained in CHANDRA LABS, Hyderabad for Analytical Testing: FT-IR Spectroscopy,

Gas Chromatography (G.C) and High Pressure Liquid Chromatography (HPLC).

Scientific presentations:

2008: Bhupesh Parise, Markus. N. Preising, B. Lorenz : Investigation on proteins interacting

with AIPL1. First GGL-Conference on Life Sciences, Giessen, Germany.

2009: Bhupesh Parise, Markus. N. Preising, B. Lorenz : Investigation on proteins interacting

with AIPL1. Second GGL-Conference on Life Sciences, Giessen, Germany.

2010: Bhupesh Parise: Investigation on proteins interacting with AIPL1. Third GGL-

Conference on Life Sciences, Giessen, Germany. (Oral presentation)

2011: Markus Preising1, Bhupesh Parise1, Birgit Lorenz1 : Overlaping Intracellular Localization

of AIPL1 and CENPF upon Heterologous Expression of His-Tagged AIPL1 Constructs. 37th

Annual Meeting of the European Paediatric Ophthalmological Society Visual Impairment in

Childhood. Thessaloniki, Greece.

2012: Bhupesh Parise, Markus. N. Preising, B. Lorenz: Overlapping Intracellular Localization

of AIPL1 and CENPF upon Heterologous Expression of His-Tagged AIPL1 Constructs. The

Association for Research in Vision and Ophthalmology (ARVO) Fort Lauderdale Florida, USA.

Publications: (Manuscript Prepared)

Alternative splicing of AIPL1 gene - implications on biological function and the mutational

spectrum.

Bhupesh Parise1, Bodo Janke2,3, Birgit Lorenz1,3, Markus N. Preising1, 3

1

Department of Ophthalmology, Justus-Liebig-University, Giessen, Germany.

Corresponding Author: Dr. rer. medic. Dipl.-Biol. Markus N. Preising,

Department of Ophthalmology

Laboratory for Molecular Ophthalmology

Justus-Liebig University

Friedrichstr. 18

35392 Giessen

Tel: +49-641-***-*****

Email: acdc8z@r.postjobfree.com

Personal Profile:

Name: Bhupesh Parise

Gender: Male

Date of Birth: 04-05-1983

Nationality: Indian

Languages known: Telugu, Hindi, Tamil, English & German (B2 level)

E-mail: acdc8z@r.postjobfree.com

acdc8z@r.postjobfree.com

Permanent Address:

Bhupesh Parise

C/O Rajkumar Vutukuri

Marienberg Strasse 5-7

Stock - 3, Zimmer - 6

60528, Frankfurt an Main

Germany.

References

Prof. Dr. med. Birgit Lorenz, FEBO

Director, Department of Ophthalmology

Faculty of Medicine

Justus-Liebig Universität

Universitätsklinikum Gießen und Marburg GmbH

Friedrichstr. 18, D-35392 Gießen

Telefon: +49-641-***-*****

E-Mail: acdc8z@r.postjobfree.com

PD Dr. rer. medic., Dipl-Biol. Markus Preising

Justus-Liebig Universität

Department of Ophthalmology

Faculty of Medicine

Friedrichstr. 18

35392 Gießen

Tel: +49-641-***-*****

Email: acdc8z@r.postjobfree.com

PD Dr. Dr. med. vet. Knut Stieger

Department of Ophthalmology

Faculty of Medicine

Justus-Liebig-University Giessen

Friedrichstrasse 18

35392 Giessen, Germany

Tel: +49-641-**-*****

Email: acdc8z@r.postjobfree.com

Dr.P. Sundaresan

Senior Scientist

Department of Genetics

Aravind Medical Research Foundation

Dr. G.Venkataswamy Eye Research Institute

Aravind Eye Hospital, No.1 Anna Nagar

Madurai-625 020, Tamilnadu, India

Phone: 045*-******* Extn:423 (office)

Email: acdc8z@r.postjobfree.com

acdc8z@r.postjobfree.com

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