Quality Control Maintenance
Resume:
Francesca J. Davis
Chicago, Illinois 60628
******************@*****.***
EDUCATION:
Ph.D. (Animal Health & Biomedical Sciences)
University of Wisconsin-Madison, USA 1999
M.V.Sc (Surgery)
Bombay Veterinary College, India 1990
B.V.Sc & A.H.
Bombay Veterinary College, India 1987
TRAINING & EMPLOYMENT:
June 2011-Currently Research Specialist, Department of Pediatrics, University
of Illinois, Chicago.
VMU technician primary function: rodent care and facility maintenance.
Primary research functions: genotyping and maintenance of a transgenic mouse colony as a model for
Alzheimer’s disease. Mouse brain tissue harvest and total protein extraction. Western analyses for
Alzheimer’s disease /synaptic markers. Quantitative densitometry of Western blot data to compare
treatment effects on the same markers. Custom sandwich and commercial colorimetric ELISAs for soluble
and insoluble Amyloid species detection. Laboratory management functions: creating and updating
inventories, purchasing, generating Standard Operating Procedure documentation and ensuring general
laboratory safety, Training new personnel in established laboratory practices, and techniques.
February 2010-February 2011 Visiting Senior Research Specialist in Health Sciences,
Department of Pharmacology, University of Illinois,
Chicago.
Primary job functions included design, planning and execution of scientific experiments to examine the role
of NF kappa B in transcriptional regulation of genes invol ved in store operated calcium entry in pulmonary
micro-vascular endothelial lining. Techniques utilized: endothelial cell culture, cellular/nuclear protein
extraction and quantization, Western blotting, Electro-mobility Gel Shift Assays (EMSA) for protein-DNA
binding; bacterial transformation and plasmid DNA purification; transient transfection of cellular mono-
layers with expression plasmid DNA/ drug treatment, Luciferase reporter assays; assisting with manuscript
and grant preparations, including graphic representation of d ata, utilizing Microsoft Office.
January 2007- January 2010 Post-doctoral fellow, Department of Biochemistry, Rush
University Medical Center, Chicago.
Primary job functions included bench research and publication of manuscripts documenting intra-cellular
signaling of bFGF within human articular chondrocytes and a review article describing the role of
sumoylation in joint disorders. Techniques utilized: primary human chondrocyte culture in monolayer and
alginate beads; Western blotting; biochemical assays for proteoglycan synthesis and quanti zation; semi-
quantitative and real time PCR for gene expression analyses; Vera Doc system densitometry analysis of
array data; PUBMED literature searches, science writing /editing in manuscript and /or peer reviewed
abstract format; Refereed scientific literature for publication in peer reviewed journals; Trained medical
and graduate students in bench techniques. Laboratory management processes included creating and
updating inventories, preparing stocks of primary cell cultures for cryopreservation, dissection and
shipment of various human joint tissues, purchasing, monitoring compliance with Rush University’s
standard for safe laboratory practices, including hazardous chemical, infectious agents, human tissue and
radio isotope usage.
November 2005-Jan 2007 Research Associate, Radiation Oncology, Loyola
University, Maywood, Illinois.
Primary job functions included bench research that lead to authorship on published manuscript(s)
addressing the patho-physiology of cell fate deter mination in cancer cells. Bench research included: Cell
culture and maintenance of several adherent and non-adherent cancer cell lines; Time- and dose-response
studies to various anticancer agents and metabolic inhibitors in cultured cells; Cellular/nuclear protein
extraction; GST-tagged protein purification; Western blotting; total RNA extraction; Reverse Transcription
and Polymerase Chain Reaction (RT -PCR) for semi-quantitative gene analyses; Primer design; Agarose gel
electrophoresis; Bacterial transformation, plasmid DNA amplification and purification; Transient
transfection of DNA by Electro-poration in mammalian cells using the Amaxa system; Gene transcription-
reporter assays; Science writing of published manuscript; Laboratory maintenance duties including routine
equipment cleaning, equipment and material inventories.
August 2005 – November 2005 Veterinary Assistant. Petsmart, Chicago.
Assisted Veterinary Technicians and Veterinarians in animal handling and restraint during routine
examination, as well as X Ray procedures. Sampling of skin, blood, fecal and urine samples; Clinical tests
for diagnostic purposes. Dispensing prescribed medication and preventives. Cage cleaning and dog
walking.
August 2002 - June 2005 Research Associate/Post-doctoral fellow / Department of
Cardio-thoracic Surgery, University of Chicago, IL.
The position supported investigations on the role of histone deacetylase enzymes in epigenetic regulation of
cardiac hypertrophy. Technical skills employed: kinase / enzyme assays; EMSAs; ELISAs; transient and
stable transfections; gene reporter assays; Western, Northern and Southern blots; RT-PCR; sub-cloning of
DNA plasmids; primary neonatal rat cardiac myocyte and cardiac fibroblasts culture; microscopy
(fluorescent, light and con-focal); rodent handling; genotyping for transgenic colony maintenance;
computer based bio-informatics software usage and data analyses.
December 1999 - August 2002 Sr. Research Technologist, Department of Cardio-thoracic
Surgery, University of Chicago, IL.
The position supported investigations on the role of Serum Response Factor in human heart failure,
utilizing cellular and molecular biology techniques, and lead to a first authorship publication. Technical
skills employed are listed above.
July 1998- July 1999 Assistant Scientist Department of Surgical Sciences, School
of Veterinary Medicine, University of Wisconsin-Madison.
The position supported investigating the role of Neuro-peptides in inflammation of the Bladder.
Techniques employed: Invitro tissue pharmacology; cell culture; Western blotting and ELISAs.
October 1998-December 1999 Quality control Specialist, Nabisco Cookie factory,
Chicago.
The temporary position was supported by ‘LabTemps’ staffing company. The job functions were quality
assurance during batch manufacture of several different production lines of Nabisco cookies/snacks.
1988 – 1990 Resident (Veterinary Surgery)
Bai Sarkarbai Dinshaw Petit Animal Hospital, Bombay .
1987 Intern, Bombay Veterinary Teaching Hospital, Bombay.
PERSONAL STATEMENT: I am highly motivated, posses a great work ethic and a keen desire to
contribute to life science research. I am very committed to making a positive difference to the advancement
of science and society as an American citizen. If given the opportunity, you can be assured that I will work
with utmost dedication to meet with your standards of excellence .
Selected peer-reviewed publications:
1. Xiao C, Davis FJ, Chauhan BC, Viola KL, Lacor PN, Velasco PT, Klein WL, Chauhan NB.
Brain Transit and Ameliorative Effects of Intranasally Delivered Anti -Amyloid-β Oligomer Antibody in
5XFAD Mice. J Alzheimers Dis. 2013 Jan 1;35(4):777 -88.
2. Neelima B. Chauhan, Francesca Davis, Chun Xiao. Wheat germ agglutinin enhanced
cerebral uptake of anti-A antibody after intranasal administration in 5XFAD mice.
Vaccine. 2011 Oct 13;29(44):7631-7.
3. Dongyao Yan, Francesca J. Davis, Andrew D. Sharrocks and Hee-Jeong Im.
Emerging roles of SUMO modification in arthritis (Review). Gene. 2010 October, 466(1 -
2), 1-15.
4. Im HJ, Li X, Muddasani P, Kim GH, Davis F, Rangan J, Forsyth CB, Ellman M,
Thonar EJ. Basic fibroblast growth factor accelerates matrix degradation via a
neuro-endocrine pathway in human adult articular chondrocytes. J Cell Physiol.
2008 May;215(2):452-63.
5. Im HJ, Muddasani P, Natarajan V, Schmid TM, Block JA, Davis F, van Wijnen AJ,
Loeser RF. Basic fibroblast growth factor stimulates matrix metalloproteinase -13
via the molecular cross-talk between the mitogen-activated protein kinases and
protein kinase Cdelta pathways in human adult articular chondrocytes. J Biol
Chem. 2007 Apr 13;282(15):11110 -21.
6. Alimirah F, Panchanathan R, Davis FJ, Chen J, Choubey D. Restoration of p53
expression in human cancer cell lines upregulates the expression of Notch1:
implications for cancer cell fate determination after genotoxic stress.
Neoplasia. 2007 May;9(5):427-34.
7. Alimirah F, Chen J, Davis FJ, Choubey D. IFI16 in human prostate cancer. Mol
Cancer Res. 2007 Mar;5(3):251-9.
8. Davis FJ, Pillai JB, Gupta M, Gupta MP. Concurrent opposite effects of
trichostatin A, an inhibitor of histone deacetylases, on expression of alpha -MHC
and cardiac tubulins: implication for gain in cardiac muscle contractility. Am J
Physiol Heart Circ Physiol. 2005 Mar;288(3):H1477 -90.
9. Davis FJ, Gupta M, Camoretti-Mercado B, Schwartz RJ, Gupta MP.
Calcium/calmodulin-dependent protein kinase activates serum response factor
transcription activity by its dissociation from histone deacetylase, HDAC4.
Implications in cardiac muscle gene regulation during hypertrophy. J Biol Chem.
2003 May 30;278(22):20047-58.
10. Davis FJ, Gupta M, Pogwizd SM, Bacha E, Jeevanandam V, Gupta MP. Increased
expression of alternatively spliced dominant -negative isoform of SRF in human
failing hearts. Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1521 -33.
11. Gupta M, Davis FJ, Jayakar D, Jeevananadam V. A dominant negative isoform of
the serum-response factor (SRF) is increased in the failing human heart. J Heart
Lung Transplant. 2001 Feb;20(2):160.
12. Gupta M, Kogut P, Davis FJ, Belaguli NS, Schwartz RJ, Gupta MP. Physical
interaction between the MADS box of serum response factor and the TEA/ATTS
DNA-binding domain of transcription enhancer factor -1. J Biol Chem. 2001 Mar
30;276(13):10413-22.
13. Rauch D, Drescher P, Pereira FJ, Knes JM, Will JA, Madsen PO. Comparison of
iodinated contrast media-induced renal vasoconstriction in human, rabbit, dog,
and pig arteries. Invest Radiol. 1997 Jun;32(6):315 -9.
14. Pereira FJ, Will JA. Functional characterization of post-junctional adrenergic
receptor subtypes in bovine intra-mammary arteries. J Vet Pharmacol Ther. 1997
Dec;20(6):434-41.
15. Pereira FJ, Drescher P, Rauch D, Madsen PO, Will JA. Comparative regulation of
alpha1-adrenergic receptor mediated contraction in urogenitally derived smooth
muscle. Effect of epidermal growth factor. Urol Res. 1997;25 Suppl 1:S13 -9.
16. Drescher P, Pereira F, Will JA, Madsen PO. Nephrotoxicity from contrast media:
attenuation with theophylline. Radiology. 1995 Nov;197(2):547 -8.
CITIZENSHIP: United States of America
REFERENCES:
Dr. Divaker Choubey, Ph.D
********@******.**.***
Tel: 513-***-****
Dr. Hee-Jeong Im Sampen, Ph.D
***-************@****.***
Tel: 312-***-**** (office)/312-***-**** (lab)
Dr. Chinnaswamy Tiruppathi,Ph.D
*****@***.***
Tel: 312-***-**** (office)/312-***-**** (lab)
Contact this candidate