Project Information Technology

AKAASH MISHRA

**** *. ************ **., ***# ***, Indianapolis, IN, 46202 USA. Email: acd9x4@r.postjobfree.com

EDUCATION

Master of Science (Biochemistry and Molecular Biology), Indiana University School of Medicine, Indianapolis, IN, USA

(GPA 3.94) (2011-current)

Master of Technology (Biotechnology), Jaypee Institute of Information Technology, Noida, Delhi NCR, India (CGPA 8.9 ~ 89%

aggregate) (2010-11)

Bachelor of Technology (Biotechnology), Jaypee Institute of Information Technology, Noida, Delhi NCR, India (CGPA 8.8 ~

88% aggregate) (2006-10)

WORK EXPERIENCE

Aug 2011- Present (~3 years): Graduate Research Assistant, Indiana University School of Medicine, Indianapolis, IN, USA

May 2009-July 2009: Summer Research Fellowship at School of Life Sciences, Jawaharlal Nehru University, Delhi, India

Project: Cloning of a Response Regulator and Transformation Of Rice cDNA Library into Yeast.

May 2008-July 2008: Summer Internship at Department of Plant Molecular Biology, South Campus, Delhi University, India

Project: Expression Analysis Of A Hydrophobic Protein From Wheat Heat Stressed cDNA Library

BIOTECHNOLOGY SKILLSET

Molecular Biology and Biochemistry Techniques – Cloning, Protein Purification, FPLC, HPLC, Electrophoretic Mobility Shift

Assay, Fluorescence Anisotropy, Yeast Two Hybrids, Co-Immunoprecipitation, RNA Isolation, RT PCR, Western Blots, Southern

Blots.

Tissue Culture Techniques- Primary cell lines from Human tissues, Transfection, Viral Transduction, Colony Formation Assays,

Flow Cytometry, MTT Assays, Luciferase Assays.

Mouse Handling- Breeding, Injecting, Checking Plugs, Cervical Dislocation, Harvesting Embryos, Cre LoxP System, Tamoxifen

Inducible Cre Conditional Knockouts.

RESEARCH PROJECTS

Year and Project Supervisor Project Organization

Duration

2012-2014 Dr. John Turchi Developing Small Molecule Inhibitors Indiana University

(Current) (Professor) Targeting Nucleotide Excision Repair School of Medicine

Medicine, Biochemistry Proteins RPA and XPA for Platinum based

Combination Cancer Therapy

2012 Dr. Anthony Firulli Identification of the cis-regulatory elements Indiana University

(6 months) (Professor) that control Hand1 gene expression in School of Medicine

Pediatrics newly differentiated neural crest cells

2012 Dr. Keith March Identifying the best AAV serotype for Indiana University

(2 months) (Professor) transducing Human Adipose Stem cells/ School of Medicine

Medicine Adipose Tissue fraction (Rotation project)

2011 Dr. JT Zhang Targeting Cancer Stem Cells by a novel Indiana University

(2 months) (Professor) BCRP (Breast Cancer Resistance Protein) School of Medicine

Pharmacology Inhibitor (Rotation project)

2010-2011 Dr. Sanjay Gupta Purification of Chikungunya envelope Jaypee Institute of

(1 Year) (Associate Professor) proteins and mapping their viral-host Information

Department of interactions (Part of AICTE and DBT Technology, Noida.

Biotechnology Govt. of India sponsored project)

2009-2010 Dr. Sanjay Gupta Mapping viral-viral envelope protein Jaypee Institute of

(1 Year) (Associate Professor) interactions of Chikungunya virus (Part of Information

Department of DBT Govt. of India sponsored project) Technology, Noida.

Biotechnology

PROFESSIONAL ACHIEVEMENTS

1st position for technical poster presentation on Stem Cells for the topic – Potential Sources of Adult Stem Cells organized at

JIIT, Noida.

2nd prize for technical paper presentation for the topic Slurry Bioreactors – an efficient approach for Bioremediation

organized at Delhi College of Engineering (DCE).

1st position for case study competition on Knowledge Management Applications in the Business World organized at JIIT,

Noida.

INTERNATIONAL POSTERS

Midwest DNA Repair Symposium, May 2014, Detroit, USA. Developing Small Molecule Inhibitors to Target

Nucleotide Excision Repair Protein XPA for Platinum Based Combination Cancer Therapy.

Akaash Mishra, Derek Woods, Silvana Dormi and John Turchi.

Midwest DNA Repair Symposium, May 2014, Detroit, USA. Synthesis and Evaluation of Small Molecule Inhibitors

of Replication Protein A.

Akaash Mishra, Silvana Dormi, Derek Woods and John Turchi.

12th International Congress on Targeted Anticancer Therapies, March 2014, Washington DC, USA. Targeting

Nucleotide Excision Repair for Novel Cancer Therapies.

Derek Woods, Akaash Mishra and John Turchi

246th ACS National Meeting & Exposition, September 2013, Indianapolis, USA. Synthesis and Evaluation of Small

Molecule Inhibitors of Replication Protein A.

Silvana Dormi, Akaash Mishra and John Turchi.

Midwest DNA Repair Symposium, May 2013, Kentucky, USA. Targeting nucleotide excision repair: Chemical

synthetic lethality and biology based combination cancer therapy

Pamela VanderVere-Carozza, Akaash Mishra, Jeremiah Hendricks, Jiyoon Lee, and John Turchi.

Genomic Instability and DNA Repair Banff Keystone Symposium, March 2013, Alberta, Canada. Targeting

nucleotide excision repair: Chemical synthetic lethality and biology based combination cancer therapy

Pamela VanderVere-Carozza, Jeremiah Hendricks, Jiyoon Lee, Akaash Mishra and John Turchi

CONFERENCES/SEMINAR

Midwest DNA Repair Conference (May 2014), Wayne State University, Detroit, MI, USA.

National conference: Biogenesis – II (2011), held at Department of Biotechnology, IILM Academy, Greater Noida.

6th International Biofuels conference, (2010) New Delhi organized by Winrock International India.

Presented a Seminar on “An Insight into the role of Structural Proteins in Alphaviruses” at JIIT, Noida (2010).

INTERNATIONAL PUBLICATIONS

M. Akaash et. al.,

High GC content: Critical parameter for predicting stress regulated miRNAs in Arabidopsis thaliana.

Bioinformation 4(4): 151-154 (2009) [Indexed in Pubmed - PMID: 20198191, Impact Factor 1.43]

Citations: Richa Gupta et. al., Bioinformation 5(3):132-135 (2010)

M. Akaash et. al., CHIKVPRO – a protein sequence annotation database for Chikungunya Virus. Bioinformation 5(1): 4-6

(2010) [Indexed in Pubmed - PMID: 21346870, Impact Factor 1.43]

SCHOLARSHIPS RECEIVED

IBMG Fellowship at Indiana University School of Medicine, IN, USA (2011-2012)

IAFBA (Indian Airforce Benevolent Association) Subroto Memorial Scholarship: Undergraduate (Professional) Scheme - Annual

scholarship for undergraduate (professional) study for 5 years.

COMPUTER KNOWLEDGE

Well versed with Windows environment, Word, Excel, & PowerPoint.

Certificate course in C & Data structures from Aptech. (New Delhi).

Bioinformatics tools like BLAST, Expasy tools, Biology Workbench, GENSCAN, CLUSTALW, SVM-PROT etc.

PERSONALITY TRAITS

Driven to turn theories into plans, Highly value knowledge, Future-oriented, Natural leadership qualities,

Excellent verbal and written communication skills, Self-confident, Decisive.

WORK AUTHORIZATION IN USA

Optional Practical Training (OPT) – 2 Years and 5 Months Work Authorization following completion of MS degree from

Indiana University. Expected start: August/September 2014

PERSONAL INFORMATION

DOB: 9th September 1989. Nationality: Indian. Languages: English (Fluent), Hindi (Fluent), Oriya (Native).

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