Mahmoud EL-Rifai

Permanent Resident of US

*** ********** **. ******** ** 01505 C: 508-***-**** Email: acd6k3@r.postjobfree.com

OBJECTIVE

Entry level full time position in Biomedical Engineering

EDUCATION

Worcester Polytechnic Institute (WPI), Worcester, MA

Bachelor of Science in Biomedical Engineering, GPA 3.5, May 2014

EXPERIENCE

Research assistant at Massachusetts Medical School Department of Biochemistry and Molecular Pharmacology

Worcester, MA, September 2013-present.

I have gotten valuable experience in chemical techniques, instrument use, reagent preparation, and general laboratory

safety training. Performed gene transfection, western blot experiments, and cell culture work.

Summer internship at Edwin L. Steele Laboratories, Department of Radiation Oncology at Massachusetts

General Hospital/Harvard Medical School, Boston, MA, summer 2013.

Genotypes mutated mice by using PCR, real-time qPCR, and FISH techniques to distinguish homozygous,

heterozygous, and wild type mice. Determined the changes of Ang-2 and VEGF expression in hypoxia over time in

vascular endothelial cells in-vitro.

LAB EXPERIENCE

• Cells: cell culture, cell transfection, cell freezing

• Amino Acids: RNA &DNA extraction, Bacterial Transformation & DNA amplification, PCR & qPCR

• Proteins: Western Blotting, ELISA, protein extraction from cells & tissue

• Staining: Immunohistochemistry (IHC), Fluorescent in situ hybridization (FISH)

• Microscopy: Confocal laser scanning microscopy, Contrast and fluorescent microscopy

• Animals: Rat, Mice anesthetics and care

SOFTWARE EXPERIENCE

• •

LabVeiw Microsoft Office suite

• ImageJ • MATLAB Software

PROJECTS

Major Qualifying Project (MQP), UMASS Medical School: “ Upconversion Nanoparticles”, Sept 2013-present

Use UCNPs to convert NIR light to visible photons, which can activate photosensitizers adsorbed on nanoparticles via

resonance energy transfer to generate Reactive oxygen species (ROS) to kill cancer cells, and to provide an alternative

to overcome hurdles of current photodynamic therapies.

Interactive Qualifying Project (IQP), WPI: “Young Adult Health Engagement”, Jan 2013-May 2013

Conducted research to develop a measure of health engagement that customized to the context of young adults

concentrating on university undergraduates, by designing survey based on reviewed literature to identify the factors

that support and inhibit health engagement among young adults.

Engineering Design, WPI, Jan 2013-March 2013

Built Semi-Permanent ECG Pads to be used in developing countries. Identify the principle steps, tools and techniques

for managing an engineering design process and to solve a specific biomedical problem. Learned the financial,

scheduling and legal issues involved in biomedical engineering design.

ACTIVITES

• Biomedical Engineering Society, WPI, May 2012 - Present

• Phi Theta Kappa (Honor Society), received completion award, March 2011 - Present

ADDITIONAL EXPERIENCE

• Part-time Arabic teacher, Alhuda Academy, Worcester, MA, Sept 2012 – present

• Driver SNAP transportation program, WPI Police Department, May 2013 – present

• Employee at, Quiet Logistics, Devens, MA, May 2013 – August 2013

Foreign Languages: Fluent in Arabic (Native)

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