Assistant Manager

Ellen B. Richardson

Curriculum Vitae

*** ******** **.

Columbia, SC 29212

Email: *******@*****.***

Cellular phone: 803-***-****

EDUCATION

University of South Carolina, Columbia, South Carolina, August 2011-May 2014

Master of Science in Biological Sciences

Emphasis in Molecular, Cellular, and Developmental Biology

Oral thesis defense completed: March 11, 2014

University of South Carolina, Columbia, South Carolina, January 2008-2011

Bachelor of Science in Biological Sciences

Minor in Chemistry

Cum Laude

PUBLICATIONS

March 2014, manuscript in preparation, "Mitochondrial replication from embryogenesis to

adulthood in DUI species, Mytilus galloprovincialis." Richardson, EB, Showman, RM.

RESEARCH EXPERIENCE

Graduate Research Assistant: Department of Biological Sciences, University of South Carolina,

Columbia, South Carolina, August 2011-May 2014

Research Summary: Double Uniparental Inheritance, termed DUI, is the process by which

mitochondrial DNA is inherited from both the paternal and maternal parent. This is a unique

mode of inheritance of mitochondrial DNA, and is found in only a few species of bivalve

mollusks. Most all other organisms follow a pattern of strict maternal inheritance of

mitochondrial DNA. In species which follow DUI, paternally inherited mitochondrial DNA

is localized only to the germs cells of male offspring, whereas maternally inherited

mitochondrial DNA is predominant in all tissue types of the female offspring and somatic

cells of the male offspring. The requirement for differential amplification of male versus

female mitochondrial haplotypes makes it an ideal model system for examining human

mitochondrial diseases where defective mitochondria out-replicate healthy mitochondria

leading to energy deficiencies particularly in muscle and neurons, leading ultimately to death.

• Laboratory: Dr. Richard Showman

• Examined timing of maternally vs. paternally inherited mitochondrial DNA replication

during embryonic stages of development in DUI species, Mytilus galloprovincialis.

• Managed regular laboratory maintenance including: animal maintenance, ordering

supplies, lab cleanup, lab safety routines, chemical inventory, and chemical Standard

Operating Procedures (SOPs)

• Supervised and trained undergraduate research assistants

Undergraduate Research Assistant: Department of Biological Sciences, University of South

Carolina, Columbia, South Carolina, June 2010-May 2011

• Laboratory: Dr. Richard Showman

• Research Summary: Involved in tracking an endoderm-specific gene, alkaline

phosphatase, in the sea urchin species, Strongylocentrotus purpuratus, using in situ

hybridization techniques

• Developed basic laboratory skills including PCR and gel electrophoresis

TEACHING EXPERIENCE

Graduate Teaching Assistant, Department of Biological Sciences, University of South Carolina,

Columbia, South Carolina, August 2011-May 2014

• Instructor of undergraduate Cellular and Molecular Biology Laboratory.

• Co-developed course and lab manual

• Taught students basic cellular and molecular biology lab techniques, including: Reverse

Transcription, Polymerase Chain Reaction, agarose gel electrophoresis, SDS-PAGE,

zymography, light and fluorescent microscopy, DNA fragmentation assays, apoptosis

assays.

• Assisted students in developing, executing, and writing about their own research projects.

• Responsible for teaching, grading, and creating assignments including: worksheets,

quizzes, and exams.

• Taught an average of 24 students per section, with two sections per semester.

• Supervised and trained undergraduate Peer Teaching Assistants

TECHNICAL SKILLS

• Microsoft Office

• Windows programs

• Reverse Transcription of RNA

• Polymerase Chain Reaction (PCR)

• real-time PCR

• digital droplet PCR

• Bio-Rad CFX Manager software

• Agarose gel electrophoresis

• Caspase 3/7 and 8 assays

• Fluorescent and confocal microscopy

• Zeiss' Zen 2011 and Axio Vision software

• DNA Fragmentation assay

• DNA isolation and purification from tissue and cells

• RNA isolation from cells

• SDS-PAGE

• Zymography

• Immunocytochemistry

• Development of fluorescently labeled and DIG-labeled DNA probes for in situ

hybridization

ACTIVITIES AND MEMBERSHIPS

Graduate Association of Biological Sciences (GABS) member, University of South Carolina,

August 2011-present

• Helped organize GABS events including guest lecture talks and lunches, departmental

speakers, and new student meet and greets.

• Member of intramural softball team

AWARDS

Life Scholarship recipient, August 2007-May 2011

REFERENCES:

1. Richard Showman, PhD

University of South Carolina

Developmental Biology

715 Sumter Street

CLS 511

Columbia, SC 20208

*******@**.***

Relationship: Principal Investigator, Committee Member-Chair

2. Lewis Bowman, PhD

University of South Carolina

Developmental Biology

715 Sumter Street

CLS 708

Columbia, SC 29208

******@****.**.***

Relationship: Graduate Advanced Cell Biology Professor

3. Margaret Cirtain, PhD

University of South Carolina

Laboratory Coordinator: Cell and Molecular Biology Laboratory

715 Sumter Street

CLS 108

Columbia, SC

********@****.**.***

Relationship: Teaching Assistant Supervisor

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