Assistant University
Resume:
Chad J. Johnson, PhD
Campus: Home/Cell:
**** *********** ***** *** ****** St
Apt.D
Madison, Wisconsin 53706 Madison,
Wisconsin 53715
Phone 608-***-**** Phone (608) 770-
9837
acd2w5@r.postjobfree.com
EDUCATION
INSTITUTION AND LOCATION DEGREE YEARS FIELD OF STUDY
Bemidji State University B.S. 1988-1993 Biology and Chemistry
University of Wisconsin, Ph.D. 1994-2003 Cell and Molecular
Madison Biology
FURTHER EDUCATION
2013-Spring Audited 2 credit Delta course at the University of
Wisconsin - Madison, "Effective Teaching with Technology"
POSITIONS and EMPLOYMENT
2000 - Spring Teaching assistant, University of Wisconsin-
Madison
Two sections of Biocore 304, Cell Biology Lab
2003 - Jan 2007 Post-Doctoral Research Associate Dept. of Animal Health
and Biomedical Sciences, University of Wisconsin-Madison
Jan 2007 - Feb 2010 Assistant Scientist Dept of Comparative Biosciences,
University of Wisconsin-Madison
Mar 2010 - March 2014 Assistant Scientist Dept of Soil Science, University
of Wisconsin-Madison
PROFESSIONAL ACTIVITIES
Peer reviewed publications:
Johnson, CJ., Johnson, J., Clayton, M., McKenzie, D. and Aiken, J. (2003).
Prion protein gene heterogeneity in free-ranging white-tailed deer within
the chronic wasting disease affected region of Wisconsin. J Wildl Dis
39:576-581.
Pak, J.W., Vang, F., Johnson, CJ., McKenzie, D. and Aiken, J. (2005).
MtDNA point mutations are associated with deletion mutations in aged rat.
Exp Gerontol 40(3):209-218.
Johnson, CJ., Johnson, J., Vanderloo, J., Keane, D., McKenzie, D and Aiken,
J. (2006). Prion protein polymorphisms in white-tailed deer influence
susceptibility to chronic wasting disease. J Gen Virol 87:2109-2114.
Russo F, Johnson CJ, Johnson CJ, McKenzie D, Aiken JM, Pedersen JA. (2009)
Pathogenic Prion protein is degraded by a manganese oxide found in soils. J
Gen Virol 90(Pt 1):275-280
Blanchong JA, Heisey DM, Scribner KT, Libants SV, Johnson C, Aiken JM,
Langenberg JA, Samuel MD. (2009) Genetic susceptibility to chronic wasting
disease in free-ranging white-tailed deer: complement component C1q and
Prnp polymorphisms. Infect Genet Evol 9(6):1329-1335
Jennelle CS, Samuel MD, Nolden CA, Keane DP, Barr DJ, Johnson C, Vanderloo
JP, Aiken JM, Hamir AN, Hoover EA. (2009) Surveillance for transmissible
spongiform encephalopathy in Toxicol Environ Health 72(17-18):1018-24.
Heisey DM, Mickelsen NA, Schneider JR, Johnson CJ, Johnson CJ, Langenberg
JA, Bochsler PN, Keane DP, Barr DJ. (2010) Chronic wasting disease (CWD)
susceptibility of several North American rodents that are sympatric with
cervid CWD epidemics. J Virol 84(1):210-215
Johnson CJ, Herbst A, Duque-Velasquez C, Vanderloo JP, Bochsler P, Chappell
R, McKenzie D. (2011) Prion protein polymorphisms affect chronic wasting
disease progression. PLoS One. 6:e17450.
Johnson CJ, Aiken JM, McKenzie D, Samuel MD, Pedersen JA. (2012) Highly
Efficient Amplification of Chronic Wasting Disease Agent by Protein
Misfolding Cyclic Amplification with Beads (PMCAb). PLoS One. 7(4):e35383.
Robinson SJ, Samuel MD, O'Rourke KI and Johnson CJ. (2012) The role of
genetics in chronic wasting disease of North American cervids. LANDES
Biosci. 6(2):153-162
Robinson SJ, Samuel MD, Johnson CJ, Adams M, McKenzie DI. (2012) Emerging
prion disease drives host selection in a wildlife population. Ecol Appl.
22(3):1050-1059
Herbst A, Johnson CJ, Hynes K, McKenzie D, Aiken JM (2013) Mitochondrial
Biogenesis Drives a Vicious Cycle of Metabolic Insufficiency and
Mitochondrial DNA Deletion Mutation Accumulation in Aged Rat Skeletal
Muscle Fibers. PLoS ONE 8(3): e59006.
Selected Presentations:
Poster: 2013 May, Banff, Canada, Prion2013, "Detection of Prion
Protein Associated Cervid Chronic Wasting Disease in
Environmental Samples"
Speaker: 2008 September, Madrid, Spain, Prion2008, "CWD susceptibility
of several north American rodents that are sympatric with cervid
epidemics"
Speaker: 2007 August, Madison, WI, Wisconsin, Department of Natural
Resources Stakeholders Meeting, two presentations "Genetic
influence of PrP variability on CWD" and "Interspecies
transmission of CWD"
Techniques utilized:
Cell culture:
Maintaining mammalian, insect and bacterial cultures, maintaining
hybridoma cell lines and collecting/purifying monoclonal antibodies,
transiently and stably transfecting/clonally selecting cells, plaque assays
and viral growth/purification
Protein Science:
Immunoprecipitation, Western Blot, protein purification, protein
extraction, ELISA, recombinant protein production, Protein Misfolding
Cyclic Amplification and Proteinase fragmentation
Molecular Biology:
PCR, Sequencing, genome walking, cloning of PCR and restriction
digest products, DNA and RNA isolation, cDNA production, restriction
fragmentation and bacterial and mammalian transformation
Microscopy:
Histology, Immuno-histo chemistry (colorimetric and fluorescent),
gold labeling and cryo-electron microscopy
Animal Work: 18 years of experience
Animal husbandry, Inoculating animals via intra-cranial, intra-
peritoneal, sub-cutaneous and oral routes, collecting ascites fluid,
dissection (large and small mammal), anesthesia and euthanasia, perfusion
and non-recovery surgery
Volunteer Work:
WDNR Tuberculosis and CWD Surveillance: 2000 and 2001
Vilas county Wisconsin, retro-pharyngeal lymph-node collection during gun
deer season
WDNR Targeted CWD Surveillance: 2002, Spring
CWD endemic area of south-central Wisconsin, collecting retro-pharyngeal
lymph-node, obex region of the brainstem and third eyelid
WDNR Northern Unit CWD Surveillance: 2002, Fall
Second in command for the northern Wisconsin counties, duties included
training volunteer samplers, sampling retro-pharyngeal lymph-node and obex
region of the brainstem, filling out accident reports, preperation and
cleanup and the telling of jokes (sampling thousands of deer necessitates a
little levity)
USDA Depopulation of CWD infected captive heard: 2006
Sampling peripheral lymph-nodes, Black Earth, Wisconsin
University of Wisconsin Department of Soil sciences: April, 2014 -
16 - 20 hours per week mentoring 2 graduate students and assisting with
research projects regarding Prion detection
References:
Judd M. Aiken, PhD Debbie McKenzie, PhD
Professor Associate Professor
Centre for Prions and Protein Folding Diseases Biological
Sciences, UAE
acd2w5@r.postjobfree.com acd2w5@r.postjobfree.com
Joel Pedersen, PhD
Professor
Soil Science, University of Wisconsin
acd2w5@r.postjobfree.com
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