Medical Assistant
Resume:
Name Zeng (Ming) Zhao
Marital status Married, two children
Citizenship U.S.A.
Address
Olney, MD 20832
Tel. 301-***-****
Cell. 301-***-****
Education
Oct, 1978-Aug, 1983 Bachelor of biomedical science. Tianjin Medical
University, China
Sep, 1985-Aug, 1990 MSc. Human pathology, Tianjin Medical University,
China
Sep, 1990-July, 1994 M.D. University of Bern, Switzerland
July, 2011-July, 2012 ASCP Cytotechnology, UPMC, Pittsburgh, PA
Professional Curriculum
May,1983-May,1988 Assistant Professor of pathology, Department of
Pathology
Tianjin Medical University, China
Aug,1988-May,1990 Associate Professor of pathology, Department of
Pathology
Tianjin Medical University, China
Aug,1990-Aug,1991 Postdoctoral researcher, Institute of Pharmacology,
University of Bern, Switzerland
Sep,1991-Oct,1996 Postdoctoral researcher, Institute of Pathology,
University of Bern, Switzerland
Oct,1996-Oct,1998 Postdoctoral researcher
Department of Psychiatry, University of Utah
Nov,1998-May,1999 Postdoctoral researcher
Department of Psychiatry, University of California Irvine
Jun,1999-Aug,2002 Assistant Research Professor
Institute for Brain Aging and Dementia
University of California Irvine
Aug,2002-Sep.2006 Senior Research Fellow
National Cancer Institute
National Institutes of Health
Sep.2006-Jul.2011 Staff Fellow, LIB/DBPAP/OVRR/CBER/USFDA
July,2012 ASCP/CT cytotechnologist (ID: 25429065, NY CT 15903), GenPath
Bioreference, Clarksburg, MD
Publications
1. R. Somogi, M. Zhao and J. W. Stucki. Modulation of cytosolic-calcium
oscillations in hepatocytes results from crosstalk among second
messengers, the synergism between the alpha-1-adrenergic response,
glucagon and cyclic AMP, and their antagonism by insulin and
diacyglycerol manifest themselves in control of the cytosolic free
calcium oscillations. Biochem J. 286:896-877 (1992)
2. M. Zhao, J. A. Laissue and A. Zimmermann. "Neuroendocrine"
differentiation in hepatocellular carcinoma (HCC): Immunohistochemical
reactivity in related to distinct tumor cell type, but not to tumor
grade. Histol Histopathol 8(4):617-626 (1993)
3. M. Zhao, NX. Zhang, ZY. Du, J. A. Laissue and A. Zimmermann. Three
types of liver cell dysplasia (LCD) in small cirrhotic nodules are
distinguishable by karyometry and PCNA labeling, and their features
resemble distinct grades of hepatocellular carcinoma (HCC). Histol
Histopathol 9(1):73-83 (1994)
4. M. Zhao, NX. Zhang, J. A. Laissue and A. Zimmermann.
Immunohistochemical analysis p53 protein overexpression in liver cell
dysplasia (LCD) and in hepatocellular carcinoma (HCC). Virchows Archiv
424(6):613-621 (1994)
5. M. Zhao, NX. Zhang, ZY. Du, J. A. Laissue and A. Zimmermann.
Immunohistochemical detection of bcl-2 protein in liver lesions: bcl-2
protein is expressed in hepatocellular carcinomas, but not in liver
cell dysplasia. Histopathology 25(3):237-245 (1994)
6. M. Zhao, K. Matter, J. A. Laissue and A. Zimmermann. Collagen type IV
in human liver grafts: Quantitative relationship to graft survival and
rejection. Transplant Proc 26(4):2211-2214 (1994)
7. M. Zhao, AM. Wheatley, RR. Friis J. A. Laissue and A. Zimmermann. Die
Expression von C-Met and TGF-beta1 mRNA korreliert mit Fibrose und
Ducktulusproliferation in der allotransplantiert Rattenleber. Schweiz
Med Wochenschr 124:1730 (1994)
8. M. Zhao, K. Matter, J. A. Laissue and A. Zimmermann. Differential
expression of Cu/Zn and Mn superoxide dismutase in alcoholic liver
disease in man: Potential protective role of Mn superoxide dismutase. J
Hepatol 21:Suppl.1, S80, P2 C8/216 (1994)
9. M. Balli, M. Zhao and A. Zimmermann. Polycystic liver disease:
Immunohistochemical characterization of cyst epithelia and
extracellular matrix. Distr Nephol 116:125-133 (1995)
10. Q. Su, M. Zhao, E. Weber, HP. Eugster and B. Ryffel. Distribution and
activity of calcineurin in rat tissues: Evidence for
posttranscriptional regulation of calcineurin B. Eur J Biochem
230(2):469-476 (1995)
11. M. Zhao, K. Matter, J. A. Laissue and A. Zimmermann. Copper-Zinc and
Manganese superoxide dismutases immunoreactivity in hepatic iron
overload liver diseases. Histol Histopathol 10(4):925-935 (1995)
12. M. Zhao, D. Zhao, AM. Wheatley, RR. Friis, J. A. Laissue and A.
Zimmermann. Expression of C-Met mRNA and TGF-beta1 mRNA correlates with
ductular proliferation and fibrosis in grafted rat livers. AASLD
Hepatology 10:504, 1995
13. M. Zhao, K. Matter, J. A. Laissue and A. Zimmermann. Tenascin and
type IV collagen expression in liver cell dysplasia and in
hepatocellular carcinoma. Histol Histopathol 11(2):323-333 (1996)
14. M. Zhao, K. Matter, J. A. Laissue and A. Zimmermann. Copper/Zinc and
manganese superoxide dismutases in alcoholic liver disease:
Immunohistochemical quantitation. Histol Histopathol 11:899-907 (1997)
15. M. Zhao and A. Zimmermann. Apoptosis in human hepatocellular
carcinoma and in liver cell dysplasia is correlated with p53 protein
immunoreactivity. J Clin Pathol 50(5):394-400 (1997)
16. C. Gapany, M. Zhao and A. Zimmermann. The apoptosis protector, bcl-2
protein, is downregulated in bile duct epithelial cells of human liver
allografts. J Hepathology 26:535-542 (1997)
17. M. Zhao and A. Zimmermann. Apoptosis in hepatocellular carcinomas
with neuroendocrine differentiation. Histol Histopathol 12:973-980
(1997)
18. M. Zhao, J. A. Laissue and A. Zimmermann. Hepatocyte apoptosis in
hepatic iron overload disease. Histol Histopathol 12:367-374 (1997)
19. M. Zhao, J. A. Laissue and A. Zimmermann. TUNEL-positive hepatocytes
in alcoholic liver disease: Aretrospective biopsy study using DNA nick
end labeling. Virchows Archiv 431:337-344 (1997)
20. M. Zhao and A. Zimmermann. Liver cell dysplasia: Reactivity for C-Met
protein, Rb protein, E-cadherin and TGF-beta1 in comparison with
hepatocellular carcinoma. Histol Histopathol 13:657-670 (1998)
21. D. Kotler, M. Zhao and F. Clayton. Effect of combination
antiretroviral therapy upon rectal mucosa HIV RNA burden and
mononuclear cell apoptosis. AIDS 12(6):597-604 (1998)
22. PM. Bennett, M. Zhao, J. Rosenthal, M. Hoff, H. Coon and W. Byerley.
Mutation screening of a neutral amino acid transporter, ASCT1, and its
potential role in schizophrenia. Psychiatr Genet. 2000 Jun;10(2):79-82
23. M. Zhao*, DH. Cribbs, AJ Anderson, BJ. Cummings, JH. Su, AJ.
Wasserman, CW. Cotman. The induction of the TNFalpha death domain
signaling pathway in Alzheimer's disease brain. Neurochem Res. 2003
Feb;28(2):307-18.
24. M. Zhao, JH. Su, Anderson AJ, A Srinivasan, CW. Cotman. Activated
caspase-3 expression in Alzheimer's and aged control brain: Correlation
with Alzheimer pathology. Brain Res 898(2):350-357 (2001)
25. M. Zhao, JH. Su, E. Head, CW. Cotman. Accumulation of caspase cleaved
amyloid precursor protein represents an early neurodegenerative event
in aging and in Alzheimer's disease brains. Neurobiology of Disease
14(2003)391-403
26. Ming Zhao and David Schrump. Microdissection and Microarray Analysis
of Epigenetic Altered Gene Profile Induced by Decitabine (DAC),
Depsipeptide FK228 (DP) from Lung Cancer Biopsies. AACR 200*-***-***
27. Hong JA, Kang Y, Abdullaev Z, Flanagan PT, Pack SD, Fischette MR,
Adnani MT, Loukinov DI, Vatolin S, Risinger JI, Custer M, Chen GA, Zhao
M, Nguyen DM, Barrett JC, Lobanenkov VV, Schrump DS. Reciprocal binding
of CTCF and BORIS to the NY-ESO-1 promoter coincides with derepression
of this cancer-testis gene in lung cancer cells. Cancer Res. 2005 Sep
1;65(17):7763-74
28. Kassis ES, Zhao M, Chen GA, Hong JA, Nguyen DM, Schrump DS. Depletion
of DNA methyltransferase(DNMT) 1 and/or 3b mediates growth arrest and
apoptosis in lung and esophageal cancer and malignant pleural
mesothelioma cells. J Thorac Cardiovasc Surg. 2006 Feb;131(2):298-306.
Epub 2006 Jan 18
29. Schrump DS, Fischette MR, Nguyen DM, Zhao M, Li X, Kunst TF, Hancox
A, Hong JA, Chen GA, Pishchik V, Figg WD, Murgo AJ, Steinberg SM.
Phase I Study of Decitabine-Mediated Gene Expression in Patients with
Cancers Involving the Lungs, Esophagus, or Pleura. Clin Cancer Res.
12: 5777-5785, 2006.
30. Zhang XH, Rao M, Loprieato JA, Hong JA, Zhao M, Chen GZ, Humphries
AE, Nguyen DM, Trepel JB, Yu X, Schrump DS. Aurora A, aurora B and
survivin are novel targets of transcriptional regulation by histone
deacetylase inhibitors in non-small cell lung cancer.Cancer Biol Ther.
2008 Jun 2;7(9).
31. Schrump DS, Fischette MR, Nguyen DM, Zhao M, Li X, Kunst TF, Hancox
A, Hong JA, Chen GA. Clinical and molecular responses in lung cancer
patients receiving Romidepsin. Clin. Cancer Res. 14: 188-98, 2008
32. Viraj P. Mane, Philippa Hillyer, Zeng Zhao, Ronald L. Rabin. A qRT-
PCR high fidelity assay for subtypes of human interferon-? and
interferon-?l: expression profiles are ligand and cell-dependent. ICB
Research Expertise
1. Surgical pathology
-Histopathological interpretation from human/mouse gross, microscopic
samples
-Tissue process of paraffin and frozen samples
-Paraffin and frozen section preparation
-Electron microscopy section preparation
-Histochemistry/immunohistochemistry on paraffin, frozen, vibratome
sections
-ISH on histological sections and white blood cells chromosomes
2. Gene profiling analyses from limited biological samples (100 to 1,000
cells)
-Laser capture microdissection from fine needle biopsy, IHC stained
slides, and flow phenotypic sorted cells
-Tissue sampling and processing to keep integrity of RNA and protein
epitopes
-Section preparation for IHC, histochemistry, LCM, and RNA/micro RNA
isolation
-Linear amplification (amplifies the only original mRNAs) and
preparation of probe for gene profiling analyses (microarray, Taqman
array, SOLiD sequencing)
3. Bioinformatic Analysis
-Affymetrix human gene ST microarray module sofware
-Clustering and statistical analysis of the array image
-Biological and molecular pathway analysis of induced genes
4. Molecular Biology
-DNA and RNA isolation from blood, cell lines, and surgical samples
-TaqMan gene expression and TaqMan array analysis (ABI7900HT)
-Northern blot of TGF-betas and C-Met from hepatomas
-Western blot of neuronal signal transduction pathway
-In situ Hybridization by use of riboprobes for oncogene expression
-In situ terminal deoxynucleotidyl transferase reaction to detect
apoptosis
-Genomic and cDNA sequencing by manual and ABI 310
-Sequence analysis with GCG software, blast, translate and motifs
-Primer designation by use of oligo 5.0 software
-Primer and oligo synthesis with ABI 394 DNA synthesizer
5. Molecular Genetics
-Genome scanning with micro-satellite markers and SNP microarray
-Linkage analysis with LOD score estimation, parametric-FASTLINK
version 2.1; nonparametric-GENEHUNTER software package
-Halpotype analysis
-Positional cloning, construct contigs with ESTs, YAC and BAC
6. Stereology, Microspectrophotometry and Con-focal Image Analysis
-Analysis of fraction volumes of liver, estimate components of
extracellular matrix, dysplastic, hyperplastic, proliferating and
apoptotic liver cells
-Karyometry of nucleic acid and nuclear morphology, quantitation of
extracellular matrix (type IV collagen and tenascin) on histologic
sections
-Image analysis of labeled neurons by use of a software "Image-Pro-
Plus"
7. Biophysics
-Quantitative microfluorometry of free calcium oscillation in rat liver
cell
-Patch clamp of calcium channel on liver cell membrane
8. Liver Perfusion and Microscopic Surgery
-Enzymatic and non-enzymatic (EDTA) liver perfusion for isolation of
single liver cell
-Orthotopic liver transplantation on Lewis and Wistar rats
Research interests
My research will focus on two projects by use of detection of "undiluted"
signal in situ: (1) understand basic aspects of pathogenesis of human
respiratory virus and apply that knowledge towards development of safer and
more effective vaccines; (2) analyze epigenetic mechanisms in cancer,
especially cancer testis gene expression in situ, uncover and define
mechanisms underlying aberrant expression of cancer-testis antigens (CTAs)
in cancer.
The goal of my research is to offer investigators specialized support for
advanced antiviral immunity and cancer molecular genetic analyses, to
develop the state-of-the-art research technology to enhance the research
efforts. My interest will also focus on advancing the core services by
training and consulting on all aspects of the technology, including
experimental design, laboratory protocols, and analysis of results. The
technical support will keep abreast of new developments of technology and
applications.
Teaching philosophy
My teaching objectives are based on evidence-based clinical problem-solving
strategies to foster critical thinking skills. I encourage student to think
in a dynamic and interactive manner in my classes by constantly challenging
them to translate the theoretical knowledge and clinical presentation
(microscopic or gross findings) into a diagnosis; while at the same time
keeping in mind the molecular mechanisms of genetics, host-pathogen
interaction, induced immune responses or evasion, and the public health
implications. As a facilitator of the learning experience, I will make the
student aware of the important role pathology plays in the profession of a
surgical pathologist and in advanced biomedical research. I would like to
pay particular attention on the student's mastering the terminology,
concepts, and mechanisms essential for the understanding of disease
processes. So that they will be able to correlate what they have learned in
my class with their clinical observations. I will try to improve my
teaching by implementing the feedback from students and faculty mentors.
Contact this candidate