Human Training
Resume:
Curriculum vitae
Melisa Andrea Soland, PhD
The last name is transitioning to Melisa Vance at the time
Post-doctoral Fellow at
Wake Forest Regenerative Medicine Institute
Winston-Salem, NC 27101 USA
Work Phone: 336-***-****
Personal Phone: 336-***-****
Fax: 336-***-****
E-mail: accntb@r.postjobfree.com
E-mail: accntb@r.postjobfree.com
EDUCATION
2006-2010. Doctor of Philosophy in Cellular and Molecular Biology.
University of Nevada, Reno. USA. (GPA: 3.94 in a scale 1-4)
Dissertation: “Modulation of mesenchymal stem cell immunogenicity through
stable expression of human cytomegalovirus proteins”, directed by Professor
Graça Almeida-Porada, M.D., Ph.D.
1996-2005. Master in Biotechnology. Department of Biochemistry and
Pharmacological Sciences. National University of Rosario. Argentina. (GPA: 7.77
in a scale 1-10)
Dissertation: “Study of the effects of genetic factors on the dislipoproteinemia
of the diabetes mellitus”, directed by Professor Adriana Almará at the Institute
of Cellular and Molecular Biology of Rosario (IBR). Department of
Biochemistry and Pharmacological Sciences of Rosario. National University of
Rosario, Argentina. The project was supported by The National Scientific and
Technical Research Council.
ACADEMIC AND RESEARCH EXPERIENCE
2011-Present Post-doctoral Fellow.
Stem cell therapies for treatment of inflammatory bowel disease using murine model
under advising of Professor Graça Almeida-Porada and supported by SENS
foundation. Encapsulation of rat stem cells with alginate biomaterials for type I
diabetes treatment. Wake Forest Regenerative Medicine Institute, Winston-Salem,
NC
Experience in: writing IUCAC animal protocols and manuscripts, performing
surgery and procedures in rodents. Experience in purification of cells from diverse
tissues: Bone marrow, cord tissue, spleen, small and large intestines and magnetic
cell sorting of specific cell populations.
2013. Undergraduate training
Summer training for senior student: John Moon, under the SENS foundation.
Presentation of the poster title: “Stem-cell based therapy for the treatment of
Inflammatory bowel disease in a murine model” at the Wake Forest Institute for
Regenerative Medicine and SENS6 conference at Queens’ College, Cambridge, UK.
2012-2013. Facilitator of Grad 713-714 at Wake Forest Graduate school (see
attached letter at the end).
2006-2010. Graduate Research Assistantship-PhD
Stem cell and microbiology project under advising of Professor Graça Almeida-
Porada and supported by NIH grant. Department of Animal Biotechnology, University
of Nevada, Reno.
Experience in: Tissue culturing of adherent or suspended cells. Molecular Biology
techniques (PCR, cloning, Sequencing, DNA, RNA and protein extraction, RT, real
time PCR). Microarrays data analysis. Elisa and its combinations with several
applications: cell proliferation and cytotoxicity assays (LDH release). Microscopy:
Transmitted display, fluorescence and confocal microscopy. Image processing in
Photoshop. Flow cytometry: up to four colors and its combination with other
applications, such as complement-mediated cytotoxicity assay. Transfection and
transduction of cells with retroviral systems. Viral infections, viral titrations.
2009. Undergraduate training
Summer training for pre-vet student: Sherina Devine, under the INBRE program.
Presentation of the poster title: “Cloning of HCMV UL16, UL18 and UL40 gene
sequences into the pQCXIN retroviral vector” at the University of Nevada, Reno.
1999-2005. Teaching assistantship award following competitive evaluation in
General and Inorganic Chemistry classes at the Department of Biochemistry
and Pharmacological Sciences of Rosario. The assistantship included teaching a
laboratory course and assisting students with problems-solving skills in General
and Inorganic Chemistry. Remunerated assistantship.
2004-2005. Teaching assistantship in Molecular Biology classes at the
Department of and Pharmacological Sciences of Rosario. The assistantship
included preparing the reagents and materials for the class and teaching students
practical molecular biology techniques. Not remunerated assistantship.
1998. Teaching assistant in General and inorganic Chemistry classes at the
admission course to the careers taught at the Department of Biochemistry and
Pharmacological Sciences of Rosario. The assistantship included problems-solving
skills in General and Inorganic Chemistry. Not remunerated assistantship.
2003-2004. Master thesis performed at the Institute of Cellular and Molecular
Biology of Rosario (IBR), Department of Biochemistry and Pharmacological
Sciences of Rosario. The assistantship was performed under supervision of
Professor Adriana Almará and supported by The National Scientific and Technical
Research Council. Rosario, Argentina.
Experience in: molecular biology techniques (PCR, DNA extraction, restriction
enzyme digestion, electrophoresis in agarose and polyacrilamide gels). Protein
expression and protein purification from bacterial cultures.
LANGUAGES
English.
2004- TOEFL exam (24 for listening, 26 for writing and 24 for reading, in a scale 1-
30 for each section, 5.0 essay writing.)
2005- GRE exam (340 for verbal, 570 for quantitative and 3.5 for analytical writing)
Italian. Studied at Secondary Bilingual School “Dante Alighieri”. Rosario. (1990-
1995)
Spanish. Mother tongue language.
AWARDS AND HONORS
Post-doctoral association travel award for 2012
2010. Martha Jones Scholarship from the Nevada Women’s fund. Reno, NV
$ 1000.
2009. E.L.Cord Scholarship from the Nevada Women’s fund. Reno, NV $ 2000.
2008. E.L. Cord Scholarship from the Nevada Women’s fund. Reno, NV $2000.
2008. Research grant awarded by the Graduate Student Association. University of
Nevada, Reno. $ 2000.
2007. Research grant awarded by the Graduate Student Association. University of
Nevada, Reno. $ 2000.
2007. Third place best oral presentation at the University of Nevada Life Science
Research Retreat at Granlibakken, sponsored by Charles River Laboratories.
Tahoe City, California. October 20-21, 2007. “Invisible mesenchymal stem cells
for transplantation”
PARTICIPATION IN SCIENTIFIC MEETINGS
Poster presentations at the 15th annual NCTERMS 2013 conference meeting. Old
Salem Visitor Center, Winston Salem.
Melisa A. Soland, Joana Boura, John Moon, Christopher Porada and Graça Almeida-
Porada.“Experience with T- cell Transfer Mouse Model of Inflammatory Bowel
Disease (IBD)”
C. Stem, M. Soland, S. Mokhtari, J. Moon, G. Almeida-Porada, C. Porada. “Finding
and Optimal Cellular Vehicle for Stem-Cell Based Gene Delivery of Factor VIII
for Correction of Hemophilia A”
Poster presentation at Wake Forest annual retreat 2013. Reynolda Campus.
Melisa Soland, Christopher Porada, Graça Almeida-Porada. “Perivascular Stromal
Cells as a Potential Reservoir and a Source of
Human Cytomegalovirus Reactivation”
Poster presentation at Wake Forest annual retreat 2012. Pinehurst, North Carolina.
Melisa Soland, Christopher Porada, Graça Almeida-Porada. “Mesenchymal Stem
Cells Engineered to Target Complement-mediated Damage in Brain Injury”
Oral presentation at the 14th annual NCTERMS 2012 conference meeting. North
Raleigh Hilton.
Melisa A. Soland, Christopher Porada, Graça Almeida-Porada “Mesenchymal Stem
Cells Engineered to Target Complement-mediated Injury”
Poster presentation at Wake Forest annual retreat 2011. Pinehurst, North Carolina.
Melisa Soland, Christopher Porada, Graça Almeida-Porada. “Modulation of
Human Mesenchymal Stem Cell Immunogenicity through Forced Expression of
Human Cytomegalovirus US Proteins”
Oral presentation at Wake Forest annual retreat 2011. Pinehurst, North Carolina.
“Modulation of Human Mesenchymal Stem Cell Immunogenicity through
Forced Expression of Human Cytomegalovirus US Proteins”
Poster presentations at 52th ASH Annual Meeting and Exposition. Orlando, Florida,
USA, December 4-7, 2010.
Melisa Soland, Evan Colletti, Mariana Bego, Chad Sanada, Christopher Porada,
Esmail D. Zanjani, Stephen St Jeor, Graça Almeida-Porada. “Modulation of
Mesenchymal Stem Cell MHC-I Complex Increases Engraftment in Vivo”
Evan J Colletti, Melisa Soland, Stephen St. Jeor, Esmail D Zanjani, Christopher D
Porada and Graca Almeida-Porada. “Genetic Modification of Human
Mesenchymal Stem Cells Enables Contribution to the Cardiac Progenitor Pool
and Developing Myocardium In Vivo”
Poster presentation at the 35th Annual International Herpesvirus Workshop. Salt
Lake City, Utah, USA. July 24th -29th, 2010.
Lisa R. Keyes, Melisa Soland and Stephen St. Jeor. “The Role of the LUNA protein
in Regulating HCMV Latency”
Poster presentation at the Combined Biochemistry/CMB/CMPP Graduate Retreat.
University of Nevada, Reno. October 17th, 2009
Melisa Soland, Christopher Porada, Esmail D. Zanjani, Stephen St Jeor, Graça
Almeida-Porada. “Novel mechanism for human cytomegalovirus evasion of the
complement system activation”
Poster presentation at 50th ASH Annual Meeting and Exposition. San Francisco,
California, USA, December 6th – 9th, 2008.
Melisa Soland, Mariana Bego, Christopher D Porada, Esmail D. Zanjani, Stephen St
Jeor and Graca Almeida-Porada. “Modulation of mesenchymal stem cell
immunogenicity through forced expression of human cytomegalovirus proteins”
Oral presentation at the University of Nevada Life Science Research Retreat at
Granlibakken. Tahoe City, California. October 20th-21st, 2008. “Invisible
mesenchymal stem cells for transplantation” (Awarded 3rd place best oral
presentation)
Poster presentation at the 33rd Annual International Herpesvirus Workshop. Estoril,
Portugal. July 27th - August 1st, 2008
Graça Almeida-Porada, Mariana Bego, Christopher Porada, Melisa Soland, Stephen
St Jeor. “Modulation of mesenchymal stem cell immunogenicity through forced
expression of human cytomegalovirus proteins”
Poster presentation at the 11th International CMV and Beta Herpesvirus Workshop.
Toulouse, France. May 13th–17th, 2007.
Mariana Bego, Melisa Soland, Stephen St Jeor. “Human Cytomegalovirus
Infection of Human Fibroblasts Cells Stably Expressing Protein LUNA”
Poster presentation at the 64th Scientific Sessions of diabetes of the “Journal of the
American Diabetes Association”. Orlando, Florida, United States June 4th–8th,
2004.
Soland M.A., Bouvet B.R., Arriaga S.M., Brufman A.S., Pezzotto S.M., Almará
A.M. “Lipoprotein Lipase, Apolipoprotein CIII and Apolipoprotein E Gene
Polymorphisms, and Hypertriglyceridemia in Type 2 Diabetes Mellitus”.
Poster presentation at the III Symposium of exposition of scientific and
technological activities. Sede de Gobierno de la Universidad Nacional de Rosario,
Argentina. November 15th -19th, 2004.
Soland M., Bouvet B., Arriaga, S., Almará A. “Estudio de la relación entre
Hipertrigliceridemia y polimorfismos genéticos de la Lipoproteína Lipasa y de
Apolipoproteínas E y CIII en diabéticos tipo 2”.
Poster presentation in V Regional Biotechnology symposium. Department of
Biochemistry and Pharmacological Sciences. Rosario, Argentina. November 25th,
2003.
Soland M., Almará A., Bouvet B “Hipertrigliceridemia y polimorfismo de
apolipoproteínas en diabéticos tipo 2”
Poster presentation in XLVIII Scientific Reunion of Argentinean society of clinic
investigation (S.A.I.C.) and Scientific reunion of immunology society (S.A.I.). Hotel
13 de julio, Mar del Plata, Argentina. November 19th – 22th, 2003.
Almará A., Soland M., Bouvet B “Hipertrigliceridemia y polimorfismo de
apolipoproteínas en diabéticos tipo 2”.
Attendance in National Congress of Biochemistry, Buenos Aires, Argentina. August
1999.
PUBLICATIONS
Melisa A. Soland, Lisa R. Keyes, Robert Bayne, John Moon, Christopher D. Porada,
Stephen St. Jeor, Graça Almeida-Porada. “Perivascular Stromal Cells as a
Potential Reservoir and a Source of Human Cytomegalovirus Reactivation”
American Journal of transplantation (Currently in press)
Almeida-Porada G, Soland M, Boura J, Porada CD. “Regenerative medicine:
prospects for the treatment of inflammatory bowel disease” Regen Med. 2013
Sep;8(5):631-44. doi: 10.2217/rme.13.52.
Soland MA, Bego M, Colletti E, Zanjani ED, St Jeor S, Porada CD, Almeida-Porada
G.“Mesenchymal stem cells engineered to inhibit complement-mediated
damage”. PLoS One. 2013;8(3):e60461.
Colletti E, El Shabrawy D, Soland M, Yamagami T, Mokhtari S, Osborne C,
Schlauch K, Zanjani ED, Porada CD, Almeida-Porada G.“EphB2 isolates a human
marrow stromal cell subpopulation with enhanced ability to contribute to the
resident intestinal cellular pool”. FASEB J. 2013 Jun;27(6):2111-21. doi:
10.1096/fj.12-205054. Epub 2013 Feb 14.
Keyes LR, Hargett D, Soland M, Bego MG, Rossetto CC, Almeida-Porada G, St
Jeor S. “HCMV protein LUNA is required for viral reactivation from latently
infected primary CD14 cells”. PLoS One. 2012;7(12):e52827. doi:
10.1371/journal.pone.0052827. Epub 2012 Dec 26.
Sanada C, Kuo CJ, Colletti EJ, Soland M, Mokhtari S, Knovich MA, Owen J,
Zanjani ED, Porada CD, Almeida-Porada G. “Mesenchymal stem cells contribute
to endogenous FVIII:c production”. J Cell Physiol. 2013 May;228(5):1010-6. doi:
10.1002/jcp.24247.
Melisa A. Soland, Mariana G. Bego, Evan Colletti, Christopher D. Porada, Esmail D.
Zanjani, Stephen St. Jeor, and Graça Almeida-Porada. “Modulation of Human
Mesenchymal Stem Cell Immunogenicity through Forced Expression of Human
Cytomegalovirus US Proteins”. PLoS One. 2012; 7(5): e36163.
Melisa Andrea Soland, Christopher D. Porada and Graça D. Almeida-Porada (2012).
“Potential of Mesenchymal Stem Cells for Liver Regeneration, Liver
Regeneration:, Pedro M. Baptista (Ed.), ISBN: 978-***-**-****-7, InTech,
Available from: http://www.intechopen.com/books/liver-regeneration/potential-of-
mesenchymal-stem-cells-for-liver-regeneration
Keyes LR, Bego MG, Soland M, St Jeor S. “Cyclophilin A is required for efficient
human cytomegalovirus DNA replication and reactivation”. J Gen Virol. 2012
Apr;9 3(Pt 4):722-32. Epub 2012 Jan 13. This work was cited by
globalmedicaldiscovery: http://globalmedicaldiscovery.com/key-scientific-
articles/cyclophilin-a-is-required-for-efficient-human-cytomegalovirus-dna-
replication-and-reactivation/
Melisa A. Soland, Cecilia V. Paparella, Beatriz R. Bouvet, Sandra M. Arriaga and
Adriana M. Almará. “Evaluation of the Relationship between Urinary
N Acetylglucosaminidase and Urinary Albumin Excretion in Diabetic
Nephropathy” 71st Scientific Sessions of the American Diabetes Association. July
2011, San Diego, California. Abstract publication: 2104-PO
Melisa Soland, Evan Colletti, Mariana Bego, Chad Sanada, Christopher Porada,
Esmail D. Zanjani, Stephen St Jeor, Graça Almeida-Porada. “Modulation of
Mesenchymal Stem Cell MHC-I Complex Increases Engraftment in Vivo”.
Abstract publication at 52th ASH Annual Meeting and Exposition. Orlando, Florida,
USA, December 4-7, 2010.
Evan J Colletti, Melisa Soland, Stephen St. Jeor, Esmail D Zanjani, Christopher D
Porada and Graca Almeida-Porada. “Genetic Modification of Human
Mesenchymal Stem Cells Enables Contribution to the Cardiac Progenitor Pool
and Developing Myocardium In Vivo”. Abstract publication at 52th ASH Annual
Meeting and Exposition. Orlando, Florida, USA, December 4-7, 2010.
Soland M., Bego M., Porada C., Zanjani E., St Jeor S. and Almeida-Porada G.
“Modulation of mesenchymal stem cell immunogenicity through forced
expression of human cytomegalovirus proteins”. Abstract publication at 50th
ASH Annual Meeting and Exposition. San Francisco, California, USA, December
6th–9th, 2008.
M.A Soland, Beatriz Reina Bouvet, S. Arriaga, Adriana María Almará. “Lipoprotein
lipase gene polymorphism and hypertriglyceridemia in patients with type 2
diabetes mellitus”. Avances en diabetología, vol. 24, Nº 3, 231-236 (2008).
Soland M.A., Bouvet B.R., Arriaga S.M., Brufman A.S., Pezzotto S.M., Almará
A.M. “Lipoprotein Lipase, Apolipoprotein CIII and Apolipoprotein E Gene
Polymorphisms, and Hipertrigliceridemia in Type 2 Diabetes Mellitus”. Journal
of the American Diabetes Association, vol. 52, Nº 2, A 521, 2189-PO (2004).
Almará A., Soland M., Bouvet B “Hipertrigliceridemia y polimorfismo de
apolipoproteínas en diabéticos tipo 2”. Medicina vol. 63, Nº 5/2, 526 (2003)
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