Manager Laboratory

Tatiana G Shapkina

**** ******* *****

Raleigh, NC 27604

919-***-**** (home)

919-***-**** (cell)

acckq1@r.postjobfree.com

SUMMARY

Highly experienced biochemist with more than twenty years of research lab expertise,

looking for an opportunity to contribute to biotech industry.

Proficient in wide variety of laboratory techniques including: ribosomes, nucleic acids

and tagged and untagged protein purification; radioactive and fluorescent labeling; preparative

and analytical gel electrophoresis, LC, HPLC, ultracentrifugation, PCR; data analysis using

Excel and Kaleidagraph programs. Familiar with stopped-flow and quench-flow techniques,

fluorescence polarization method, Alpha-screen, LANCE.

EXPERIENCE

NC State University – Raleigh, NC September 1996 – August 2013

Department of Molecular and Structural Biochemistry, Laboratory of Professor P. Wollenzien

Research Specialist

• Involved in numerous projects investigating the structure and structure-function

relationship of bacterial ribosomes and ribosomal ligands using various biochemical

approaches such as UV-crosslinking, foot- and toe-printing, filter-binding assay, kinetic

and thermodynamic studies of complex formation by rapid quenching and stopped flow

methods.

• Purified multiple protein factors and substrates of protein synthesis machinery for use in

laboratory experiments.

• Participated in assay development and implementation for small biotech companies:

High Throughput Screening of chemical compounds as inhibitors of protein synthesis

in Staphylococcus aureus (TRANA Discovery, Inc., Cary, NC).

This involved setting up and testing assays with Escherichia Coli and Staphylococcus

aureus ribosomes using ALPHA-screen, LANCE and fluorescence polarization

systems.

Biochemical characterization and determination of specific sites of action of

compounds selected by the above mentioned HTS and analysis of their footprints on

Staphylococcus aureus ribosomes.

Assessment of DNA– lipoprotein complex formation (ALERA Labs, LLC., Research

Triangle Park, NC).

Developed fluorescence anisotropy assay and used it to analyze interaction of DNA-

oligo with proprietary lipoprotein in aqueous solutions.

• Performed lab manager duties such as overseeing safety, radiation inventory and

personnel training, ordering, and equipment maintenance.

• Trained undergraduate and graduate students in sample preparation and analytical

methods.

Nuclear Physics Institute – St. Petersburg Russia June 1995 – August 1996

Department of Molecular and Radiation Biophysics, Laboratory of Protein Synthesis

Scientist

• Finished work on and defended doctoral dissertation “Puromycin reaction of A-site

bound peptidyl-tRNA”

• Participated in a large scale preparation of individual tRNAs for commercial use.

North Carolina State University – Raleigh, NC June 1992 – May 1995

Department of Molecular and Structural Biochemistry, Laboratory of Professor P. Wollenzien

Visiting Scientist

• Studied the structure of mRNA-70S ribosomal complexes by UV-crosslinking of s 4U-

labeled mRNA and rRNA.

Leningrad Nuclear Physics Institute – Russia September 1983 – May 1992

Department of Molecular and Radiation Biophysics, Laboratory of Protein Synthesis

Junior Scientist April 1985 – May 1992

• Purified and labeled individual tRNAs for the laboratory use.

• Studied interaction of tRNAs with bacterial ribosomes.

Intern September 1983 – March 1985

• Learned laboratory techniques, studied interaction of tRNAs with mammalian ribosomes.

EDUCATION

Engelhardt Institute of Molecular Biology – Moscow, Russia February 1995

PhD, Molecular Biology

Leningrad Polytechnical Institute – Leningrad, Russia February 1985

(now St.Petersburg State Polytechnical University)

BS/MS, Biophysics – Department of Physics and Mechanics

Master of Science theses “Interaction of deacylated tRNA with rat liver ribosomes.”

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