Development Training

Sanjeeva Sudhakar Metikala

Halberstädter straße - 4, 06112 Halle (Saale), Germany accj3y@r.postjobfree.com-halle

Work: +49 (0-345-***-**** Mobile: +491********** Sex: Male DOB: 07 August 1988

Nationality: Indian.

EDUCATION

Ph.D. in Molecular Biology & Biochemistry (current)

Institute of Physiological Chemistry, MLU, Halle-Wittenberg, Germany, 2012-current

Masters in Biomedical Engineering

Martin Luther University, Halle-Wittenberg & FH Anhalt, Köthen, Germany, 2012

Bachelors in Biotechnology

Vinayaka Missions University, Chennai, India, 2009.

Higher secondary school (Biology)

Raos Junior college, Nandyal, India, 2005.

RESEARCH EXPERIENCE

Ph.D. Thesis - “The effect of hypoxia during vertebrate development”.

Experience in working with animal model “Xenopus laevis”.

Mastered various molecular, developmental biology and biochemistry techniques.

Extensive research in unfolding the effects of hypoxia on hematopoiesis and general

development.

Master’s Thesis - “The effect of hypoxic conditions on the angiogenesis during Xenopus laevis

development”.

Successfully established novel computer-controlled hypoxia culture system to study

Xenopus laevis development.

Preliminary analysis of the effect of hypoxia on embryonic vasculogenesis.

PHD-2 loss-of-function experiments.

Internship - Institute for Physiological Chemistry, MLU, Halle (Saale), Germany, 2011.

Hands-on experience with various molecular / developmental biology techniques.

Bachelor’s Thesis - “Screening of Gene polymorphisms at the Human leukocyte antigen (HLA) – DQ

A1 and Alcohol dehydrogenase (ADH) gamma gene loci in a Student

Community by PCR-SSCP”.

Hands-on exposure to basic molecular biology techniques.

Identified 3 different HLA DQ-A1 alleles (0101, 0102 and 0103 in heterozygous and

homozygous combinations) in a mixed student community in India using PCR, LIS-

SSCP.

No polymorphisms identified regarding ADH gamma gene.

Electrophoresis Techniques - Training program at “Biotech”, Yercaud, India, 2007.

Hands-on experience with various electrophoresis techniques.

TEACHING EXPERIENCE

Lecturer in Zoology - Kankara Gurreddy Junior College, Banaganapalli, India, 2009-10.

Voluntarily taught basics in Zoology to higher secondary students.

PRESENTIONS / PARTICIPATIONS

Poster presentation on “The effect of hypoxia during vertebrate development” at Sensing and

Signaling of Hypoxia: Interfaces with Biology and Medicine, Breckenridge, Colorado, USA,

Jan 2014.

Poster presentation on “The effect of hypoxia during vertebrate development” at

2. Doktoranden Kongress, Halle Saale, Germany, Nov 2013.

Oral presentation on “The effect of hypoxia during vertebrate development” at First German

Xenopus Meeting, Hohenheim, Germany, Oct 2013.

Oral & poster presentation on “The effect of hypoxia during vertebrate development” at

Molecular life sciences, Frankfurt, Germany, Oct 2013.

Poster presentation on “The effect of hypoxia during vertebrate development” at

Developmental Biology, Lucca (Barga), Italy, June 2013.

Poster presentation on “The effect of hypoxic conditions on the angiogenesis during Xenopus

laevis development”, at 9th GfE Summer School, Günzburg, Germany, Sep 2012.

Participated in 17th Leipziger Workshop on “Cytomics”, Leipzig, Germany, Jan 2011.

Actively participated in the International Conference, “Bio-Explore”, organized by Ohlone

College, U.S.A, Chennai, India, Feb 2008.

HONORS / AWARDS

Served as President, 2008-09, BIOWIN Association, Department of Biotechnology – VMU.

First prize in Electrophoresis training program, “Biotech”, Yercaud, India.

PROFESSIONAL SOCIETIES

der Gesellschaft für Biochemie und Molekularbiologie (GBM)

(German Society for Biochemistry and Molecular Biology)

Gesellschaft für Entwicklungsbiologie (GfE)

(German Society for Developmental Biology)

SELECTED SKILLS & TECHNIQUES

Molecular Biology Techniques:

• RNA and DNA isolation • DNA sequencing • cDNA synthesis

• PCR, primer construction and cloning • RT-PCR • Immunostaining

• Whole mount in situ hybridization • DNA mutagenesis using SOMA technique

• Morpholino design & in vivo application • Apoptosis screening

• Loss/Gain of function technique • LIS – SSCP • Silver Staining

• Western blot analysis • SNP

Microbiology Techniques:

• Preparation of Media and Culture • Sterilization • Inoculation

• Maintenance • Cell Staining and Counting Techniques

Biochemical Techniques:

• Assay of enzymes, nucleic acids, proteins • HPLC

• Thin Layer Chromatography

Immunology Techniques:

Immunohistochemistry, Immunodiffusion, Haemagglutination and ELISA

Computer skills:

Good command of office suite (word processor, presentation software, spread sheet)

Good command of image processing/ editing software (ImageJ, Adobe suite)

Bioinformatics:

• GenBank searching: BLAST, PSI-BLAST • Multiple sequence alignments

• Wide use of Xenbase website • in silico alignments (e.g serial cloner)

LANGUAGES KNOWN

• English – proficient user • Telugu – Mother tongue • German – Basic • Hindi – Very good user

PUBLICATIONS

Herbert Neuhaus, Sanjeeva Metikala and Thomas Hollemann. Xenopus cadherin 5 is specifically

expressed in endothelial cells of the developing vascular system. Manuscript submitted to International

Journal of Developmental Biology.

Sanjeeva Metikala, Herbert Neuhaus, and Thomas Hollemann. The effect of hypoxia on

vasculogenesis during early embryonic development of Xenopus laevis. Manuscript under preparation.

PERSONAL REFEREES

Prof. Dr.rer.nat. Thomas Hollemann, Dr. Rekha Govindan,

Martin-Luther Universität, Halle-wittenberg, Associate Professor,

Medizinische Fakultät, Department of Biotechnology,

Institut für Physiologische Chemie, Vinayaka Missions University,

Paiyanoor – 603104,

Hollystraße 1,

06114 Halle (Saale), Germany. Tamilnadu, India.

Tel : +91 (0) 44 – 27443801

Tel : +49 (0-345-***-****

Fax : +91 (0) 44 - 27443800

e-mail : accj3y@r.postjobfree.com e-mail : accj3y@r.postjobfree.com

Prof. Dr. rer. nat. Juergen Dittmer, Dr. Herbert Neuhaus,

Martin-Luther-Universität Halle-Wittenberg, Martin-Luther Universität, Halle-wittenberg,

Universitaetsklinik und Poliklinik für Medizinische Fakultät,

Gynaekologie, Institut für Physiologische Chemie,

FG5/E01, Ernst-Grube-Str. 40, Hollystraße 1,

06120 Halle (Saale), Germany. 06114 Halle (Saale), Germany.

Tel: +49 (0-345-***-**** Tel : +49 (0-345-***-****

e-mail : accj3y@r.postjobfree.com e-mail : accj3y@r.postjobfree.com

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