Assistant University
Resume:
Curriculum Vitae Dr Krista A Spreng
Krista A. Spreng Ph.D.
www.linkedin.com/pub/krista-spreng-phd/88/4b6/62b/
Department of Microbiology 12612 Grey Eagle Court #44
Uniformed Services University of the Health Sciences Germantown, MD 20874
4301 Jones Bridge Road 440-***-****
Room B4082 acchq8@r.postjobfree.com
Bethesda, MD 20814
Phone: 301-***-****
Fax: 301-***-****
acchq8@r.postjobfree.com
EDUCATION
University of Missouri, Columbia, MO 2006-2012
Ph.D. in Microbiology in the laboratory of Dr. George C. Stewart
Niagara University, Niagara University, New York 2002-2006
Bachelor of Science in Biology, minor in chemistry
RESEARCH EXPERIENCE
Post Doctoral Research Fellow: In the laboratory of Dr. Alison O’Brien, Department of
Microbiology, Uniformed Services University of the Health Sciences, MD
July 2012-
• Investigations into the activation of small GTPases such as RhoA by cytotoxic necrotizing
factor 1 from Escherichia coli
• Determination of the infectious dose of E.coli strain CP9 in a C57BL/6 mouse model
The impact NOD has on the pathogenesis on E. coli infections, performed using
o
NOD deficient mice (collaboration project)
• Determination of specific genes that contribute to pathogenesis of the uropathogenic E.
coli strain CP9 using collaborative cross mice
Doctoral Research: In the laboratory of Dr. George C. Stewart, Department of Molecular
Microbiology and Immunology and Veterinary Pathobiology (MMI/VPB), University of Missouri,
MO
2006-2012
• Identification and analysis of spore associated proteins from Bacillus anthracis identified
using transposon mutagenesis (Tn916), BSL2
Development and optimization of the transposon mutagenesis screening system
o
based off of BclA incorporation
Identification of protein interactions
o
Identification of new spore associated proteins, purification of recombinant
o
proteins, and antibody production
• Diagnostic detection of Bacillus anthracis using bioluminescent reporter phage
(collaboration), BSL3
• Characterization of collagen-like Bacillus anthracis genes 2280 and 2281
• Creation of constructs for heavy metal resistance (arsenic, cadmium, mercury, etc) to
create strains to study for bioremediation (collaboration)
Undergraduate research projects: In the lab of Dr. Mark Gallo, Niagara University, NY
2004-2006
• Development and optimization of microarray slides to study genes in Staphylococcus
aureus
Antibiotic resistant strains swabbed from the nose of deer to look at gene
o
upregulation (collaboration)
• Epidemiology study of the different Influenza strains
Undergraduate research projects: In the lab of Dr. Bill Edwards, Niagara University, NY
Curriculum Vitae Dr Krista A Spreng
2003-2004
• Collection and identification of the different algae species in the western basin of Lake Erie
HONORS AND AWARDS
• International Gram positive pathogens conference, travel award 2010
• International Gram positive pathogens conference, travel award from the post doctoral
fellow and graduate student association 2010
• First place- biology division poster contest, Central States Microscopy and Microanalysis
Society 2009
• First place- colorized transmission electron micrograph, University of Missouri Life
Sciences Week 2009
PROFESSIONAL AFFILIATIONS
• Member, American Society for Microbiology 2006- present
• Member, Microscopy Society of America 2007-present
• Member, Central States Microscopy and Microanalysis Society 2009-2012
POSTER PRESENTATIONS
• Krista A Spreng, George C Stewart (2013). Identification of an inosine-uridine preferring
nucleoside hydrolase family protein as a BxpB-dependent exosporium protein of Bacillus
anthracis, The international conference on Bacillus anthracis, B. cereus, and B.
thuringiensis, Victoria, Canada.
2013
• Krista A Spreng, George C Stewart (2010). The Contribution of an Inosine-Uridine
nucleoside preferring hydrolase family protein to spore development, Great Plains
Infectious Disease Conference, Kansas
2010
• Krista A Spreng (2010). The Contribution of an Inosine-Uridine nucleoside preferring
hydrolase family protein to spore development, International conference for Gram positive
pathogens, Omaha, Nebraska 2010
• Spreng, Krista A and Stewart, George C (2010). BAS4961: Contribution to exosporium
assembly or interaction with host/environment? Life Science Week, University of Missouri
2010
• J. Binkley, BM Thompson, K. A. Spreng, GC Stewart (2009). Creation of a better anthrax
vaccine: expression of protective antigen on the outside of a Bacillus spore? 8th annual
Great Plains Infectious Disease meeting, Kansas 2009
• Krista A Spreng, Brian M Thompson, George C Stewart (2009). Transposon mutagenesis
based study of spore maturation of Bacillus anthracis Central states microscopy and
microanalysis society, Missouri 2009
TEACHING AND TRAINING ACTIVITIES
• Teaching assistant, Microbiology course, School of Medicine, Uniformed Services
University of the Health Sciences, Bethesda, MD 2012-2013
• Teaching and training of graduate rotational students (3), Department of Microbiology,
University of Missouri, MO 2011-2012
• Teaching and training of undergraduate students (2) and technicians (1), Department of
Microbiology, University of Missouri, MO 2010-2012
• Teaching assistant, Medical Microbiology course, Department of Medicine, University of
Missouri, MO 2007-2008
• Teaching assistant, Microbiology laboratory course (2 semesters), Department of Biology,
Niagara University, NY 2005-2006
PUBLICATIONS
Spreng KA, Smith MA, Ventura CL, and O’Brien AD (2014). Genotype is an important contributing
factor of host susceptibility to urinary tract infections. In preparation.
Spreng KA and Stewart GC (2014). Identification and characterization of Bacillus anthracis spore
associated protein 4961. J Bacteriol, In preparation.
Shofield D, Sharp NJ., Vandamm J, Molineux IJ, Spreng KA, Rajanna C, Westwater C and
Stewart, GC (2013). Bacillus anthracis diagnostic detection and rapid antibiotic susceptibility
determination using ‘bioluminescent’ reporter phage. J Microbiol Methods, 95 (2): 156-61.
Curriculum Vitae Dr Krista A Spreng
Spreng KA, Thompson BM, and Stewart GC (2013). A genetic approach for the identification of
exosporium assembly determinants of Bacillus anthracis. J Microbiol Methods, 93 (1): 58-67.
Thompson BM, Hsieh HY, Spreng KA, Stewart GC (2011). The co-dependence of BxpB/ExsFA
and BclA for proper incorporation into the exosporium of Bacillus anthracis. Mol Microbiology, 79
(3): 799-813.
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