Project Medical

CURRICULUM VITAE

Zhenhua Huang, Ph.D

VISA STATUS: I have Permanent Resident Card (Green card)

CONTACT INFORMATION:

Department of Urology and Pathology, Johns Hopkins University

Email: acch45@r.postjobfree.com

EDUCATION

DEGREE

PhD: Biochemistry and Molecular Biology. Sep/1998 - Aug/2003

National Laboratory of Biomacromolecules, Center for Molecular

Biology, Institute of Biophysics, Chinese Academy of Sciences,

Beijing, China.

Dissertation: Purification, Activity and Conformation of Multidrug

Resistance Protein 1 (MRP1) and its Multidrug Resistance.

PROFFESSIONAL EXPERIENCE

Research Associate (8/2011-Current)

Department of Urology and Pathology. Johns Hopkins University Medical

School, Baltimore, MD

Research Project --The pathogenic effects of cPLA2 in prostate development

and carcinogenesis

o Studying the role of cPLA2 in prostate development.

o Studying the functions of cPLA2 in human and mouse prostate

tumor.

Research Project--Optimization of exogenous gene expression by using

lentivirus vectors

o Optimized many conditions to improve the infected

efficiencies of lentivirus.

o Constructed a co-expressing lentiviral plasmid to track the

desired gene expression.

Postdoctoral Fellow (08/2006-7/2011)

Department of Urology and Pathology, Johns Hopkins University Medical

School, Baltimore, MD

Research Project--Roles of the transcriptional activator Sox9 in prostate

growth control and cancer

o Studying mechanisms of Sox9 in control prostate

development.

o Studying the role of Sox9 in control human prostate

carcinogenesis.

o Studying the effect of Sox9 on prostate cell proliferation

suggest the encourage possibility on approach to the

treatment of prostate cancer.

Research Project--Studies the molecular mechanisms of prostate development:

o Studying the molecular mechanisms of prostate development.

o Searching candidates regulating prostate development by

using cDNA Microarry.

Postdoctoral Fellow (02/2004-07/2006)

Department of Biochemistry, Boston University, Boston, MA.

Research Project--Studies on the differentiation human embryonic stem cells

into epithelial lineage.

o Developed a highly efficient procedure to direct human

embryonic stem cell to differentiate into functional

epithelial lineage. The differentiated epithelial cells

might be used to replace the damaged epithelial cells,

especially skin.

Research Project--Studies on the effect of Badykinin and Angiotensin on the

expression of connective tissue growth factor human embryo lung fibroblasts

IMR-90 cells.

Doctoral Research Assistant (08/1998-08/2003)

National Laboratory of Biomacromolecules, Center for Molecular Biology,

Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Research Project--Studies on the mechanisms of MRP1 (multidrug resistance

protein 1) and the interaction between MRP1 and phospholipids as well as

anticancer drugs

Research Project--Studies on the mechanisms of multidrug-resistance of a

cisplatin-resistance cell line (A549/DDP).

HONORS AND AWARDS

. 2011 3rd reward of outstanding poster in 6th Annual prostate research

day in Johns Hopkins.

. 2005 Training grant on human embryonic stem cell (hESC) culture

techniques at Johns Hopkins University.

. 2003 Travel Award for 19th IUBMB Congress held in conjunction with the

2nd Annual Human Proteome Organisation (HUPO) Congress in Montreal,

Canada, 8-11 October 2003.

. 2003 Travel Award for 8th China and oversea bio-membrane Symposium

held in Beihai, P.R.China.

. 2002 Granted travel award to attend the Ninth National Biophysics

Symposium held in Dalian, P.R.China.

. 2001 "DIAO" award, Chinese Academy of Sciences (5% in total

postgraduate).

. 2001 Granted travel award to attend Biochemistry and Molecular Biology

Annual Meeting in China held in Shanghai China.

. 1997 Granted "outstanding instructor" in Huaihua medical college.

. 1996 Third prize in "teaching tournament" hold in Huaihua medical

college.

PUBLICATIONS

Peer-Reviewed Journal Publications:

Submitted, under review and in preparation

1. Zhenhua Huang, Rebecca Miller, Schaeffer EM. Optimization of

exogenous gene expression delivered by lentivirus vectors. (In

preparation).

2. Zhenhua Huang, Rebecca Miller, Schaeffer EM. Cytosolic

phospholipase A2-alpha plays a critical role in high grade human

prostate cancer.

3. Zhenhua Huang, Schaeffer EM. The functions of RNASEH2A in human

prostate cancer.

Published and accepted

1. Simons BW, Hurley PJ, Huang Z, Ross AE, Miller R, Marchionni L, Berman

DM, Schaeffer EM. Wnt signaling though beta-catenin is required for

prostate lineage specification. Dev Biol. 2012 Nov 15;371(2):246-55.

2. Hurley PJ, Marchionni L, Simons BW, Ross AE, Peskoe SB, Miller RM,

Erho N, Vergara IA, Ghadessi M, Huang Z, Gurel B, Park BH, Davicioni

E, Jenkins RB, Platz EA, Berman DM, Schaeffer EM. Secreted protein,

acidic and rich in cysteine-like 1 (SPARCL1) is down regulated in

aggressive prostate cancers and is prognostic for poor clinical

outcome. Proc Natl Acad Sci U S A. 2012 Sep 11;109(37):14977-82.

3. Huang Z, Hurley PJ, Simons BW, Marchionni L, Berman DM, Ross AE,

Schaeffer EM. Sox9 is required for prostate development and prostate

cancer initiation. Oncotarget. 2012 Jun;3(6):651-63.

4. Schaeffer EM, Marchionni L, Huang Z, Simons B, Blackman A, Yu W,

Parmigiani G, Berman DM. Androgen-induced programs for prostate

epithelial growth and invasion arise in embryogenesis and are

reactivated in cancer. Oncogene. 2008 Dec 4;27(57):7180-91.

5. Zhenhua Huang, Yu J, Toselli P, Bhawan J, Sudireddy V, Taylor L,

Polgar P. Derivation of human embryonic stem cells into epithelial

lineage leading to keratinocyte formation accompanied by de novo

expression of angiotensin II type 1 and bradykinin B2 receptors. J

Cell Physiol. 2007 Jun;211(3):816-25.

6. Yu J, Lubinsky D, Tsomaia N, Zhenhua Huang, Taylor L, Mierke D,

Navarro J, Miraz O, Polgar P. Activation of ERK, JNK, Akt, and G-

protein coupled signaling by hybrid angiotensin II AT1/bradykinin B2

receptors expressed in HEK-293 cells. J Cell Biochem. 2007 May

1;101(1):192-204.

7. Zhenhua Huang, Linda Taylor, Bin Liu, Jun Yu, and Peter Polgar.

Modulation by bradykinin of angiotensin type 1 receptor-evoked RhoA

activation of connective tissue growth factor expression in human lung

fibroblasts. American Journal of Physiology - Lung Cellular and

Molecular Physiology. 2006 Jun;290(6):L1291-9.

8. Zhenhua Huang, Youguo Huang. The change of intracellular pH is

involved in the cisplatin-resistance of human lung adenocarcinoma

A549/DDP cells. Cancer Investigation. 2005;23(1):26-32.

9. Zhenhua Huang, Chang X, Riordan JR, Huang Y. Fluorescent modified

phosphatidylcholine floppase activity of reconstituted multidrug

resistance-associated protein MRP1. Biochimica et Biophysica Acta

(BBA) - Biomembranes . 2004 Jan 28;1660(1-2):155-63.

10. Zhenhua Huang, Youguo Huang. The relationship between MRP1 activities

and its NBD conformational changes. Science in China Series C: Life

Sciences. 2004 Oct;47(5):425-33.

11. Zhenhua Huang, Tong Y, Wang J, Huang Y. NMR studies of the

relationship between the changes of membrane lipids and the cisplatin-

resistance of A549/DDP cells. Cancer Cell International. 2003 Apr 8;

3(1):5.

12. Zhenhua Huang, Youguo Huang. Changes of the pHi and [Ca2+]i are

responsible for anti-apoptotic character of A549/DDP cells. Progress

in Biochemistry and Biophysics, 2001; 28 (5), 722-727.

13. Liang X J, Zhenhua Huang, et al. Changes of cell cycle and resistance

of A549 cells to cisplatin. Progress in Biochemistry and Biophysics,

2000, 27(6): 616-620.

Abstracts:

1. Zhenhua Huang Marchionni L, David Berman, Schaeffer EM. Roles for

the Transcriptional Activator Sox9 in Prostate Growth Control and

Cancer. Abstract: Multi-Institutional Prostate Cancer Meeting -

April 12 - 14, 2010, Ft. Lauderdale, Florida USA

2. Zhenhua Huang. Purification, Activity and Conformation of MRP1 and

its Multidrug Resistance. Ph.D. dissertation. Aug, 2003.

3. Zhenhua Huang. NMR Studies of the Relationship Between the Changes

of Membrane Lipids and the Cisplatin-resistance of A549/DDP Cells.

Abstract. 19th IUBMB Congress held in conjunction with the 2nd

Annual Human Proteome Organisation (HUPO) Congress, July, 2003.

4. Zhenhua Huang, Youguo Huang. The change of intracellular pH is

involved in the cisplatin-resistance of human lung adenocarcinoma

A549/DDP cells. Abstract: 8th China and oversea bio-membrane

Symposium held in Beihai, P.R.China, Mar, 2003.

5. Zhenhua Huang, Youguo Huang. Purify MRP1 and its interaction with

anti-cancer drugs. Abstract: 8th China and oversea bio-membrane

Symposium held in Beihai, P.R.China, Mar, 2003.

6. Zhenhua Huang, Tong Y, Wang J, Huang Y. NMR studies of the

relationship between the changes of membrane lipids and the

cisplatin-resistance of A549/DDP cells. Abstract: The Ninth

National Biophysics Symposium held in Dalian, P.R.China, May, 2002.

7. Zhenhua Huang, Youguo Huang. Changes of the pHi and [Ca2+]i are

responsible for anti-apoptotic character of A549/DDP cells.

Abstract: Biochemistry and Molecular Biology 8th Annual Meeting in

China held in Shanghai China, Sep, 2001.

Research Support

Ongoing Research Support:

1. Project Title: The pathogenic effect of cytosolic phospholipase C alpha

in prostate carcinogenesis

Award Agent: Howard Hughes Medical Institute

PI: Edward Schaeffer Currently (2010-1015)

Total Grant Amount: $375,000, Current Year Amount $75,000.

Role: Postdoctoral fellow:

Edward Schaeffer (PI) Currently (2008 to 3/2013)

2. Project Title: Roles for Phospholipase A2 Signaling in Prostate

Development

Award Agent: Federal Government

PI: Edward Schaeffer.

Total Grant Amount: $728,000, Current Year Amount: $145,600

Role: Postdoctoral fellow

3. Project Title: Study on Hedgehog pathway expression in prostate cancer

progosis.

David Berman (PI) Currently.

Role: Postdoctoral Fellow

Completed Research Support:

1. Project Title: Roles for the Transcriptional Activator Sox9 in Prostate

Growth Control and Cancer

Award Agent: Patrick Walsh Prostate Cancer Research Fund

PI Edward Schaeffer. 2008-2009

Total Grant Amount: $150,000, Year Amount: $75,000

2. Project Title: Study on differentiation human embryonic stem cell.

Peter Polgar (PI) 08/2005 - 07/2006

Role: Postdoctoral Fellow

3. Project Title: Study on the CTGF regulation in human lung embyonic

fibroblasts.

Peter Pogar (PI) 03/2004 - 07/2005

Role: Posdoctoral Fellow.

4. Study on the multidrug resistance of cancer cells.

Youguo HUANG (PI) 09/1998-07/2003

Role. Ph.D student.

Technologies:

I am good at many technologies required in basic research including cell

(animal, human, embryonic stem cell) culture, molecular biology,

biochemistry, embryology, cell biology and animal experiments et. al.

Contact this candidate