Medical Assistant

CURRICULUM VITAE

Natallia Dziuba

HOUSTON, TEXAS

Cell phone: 409-***-****

E-mail: ********@*****.***

EDUCATION:

**** – present Associates in Arts; College of the Mainland, Texas City, Texas.

2004 – 2011 Biomedical Scientist; School of Biomedical Sciences, UTMB,

Galveston, Texas.

1997 – 2004 M.D.; General Medicine. Belarussian State Medical University,

Minsk, Republic of Belarus.

Graduate study: The effect of low-energy laser treatment on lipid

peroxidation in the blood of patients with allergic skin diseases

PROFESSIONAL APPOINTMENTS AND EXPERINECE:

2011 – present Substitute Teacher. Clear Creek Independent School District,

League City, Texas. Teaching, curriculum development,

curriculum design, lesson planning, educational technology,

classroom management, adult education, instructional design.

2007 – 2011 Lab Director Assistant. Department of Internal Medicine/Infectious

Diseases, University of Texas Medical Branch, Galveston, Texas,

USA.

Planning, organizing, monitoring, coordinating, assessing and

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improving the day-to-day operation in the cell/tissue culture

facilities, chemical and biosafety laboratory.

Negotiating with vendors, replying to the phone calls and making the

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phone calls

Scanning and filing the documents, entering the data into the

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spreadsheets, performing statistics calculations

Training the lab staff on the good laboratory practices for use of

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tissue culture equipment and the biosafety rooms

Training the lab staff on proper chemical safety techniques

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Interfacing with Environmental Health and Safety, assuring

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compliance with state and federal regulations

Interacting with faculty members managing and controlling the

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equipment inventory of tissue culture and biosafety labs

Managing proper usage, timely repairs and annual servicing of lab

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equipment

Working as a tissue culture room technician, maintaining tissue

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culture inventory

2007 – 2011 Postdoctoral Associate. Department of Internal Medicine/Infectious

Diseases, University of Texas Medical Branch, Galveston, Texas,

USA.

High-throughput screening and identification of cell factors required

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for replication of HIV-1 and other viruses in primary human

macrophages

Investigating the role of CD63 and other tetraspane transmembrane

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proteins in HIV infection by siRNA transfection in cell lines.

Carried out experiments with primary human macrophages to look at

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the traffic of CD63 inside of the cell upon infection with HIV.

Expression of CD81 protein as a potential target for oligonucleotide

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type drug development

Animal handling and maintenance

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2005 – 2007 Postdoctoral Fellow. Department of Pathology, Center for

Biodefense and Emerging Infectious Diseases, University of Texas

Medical Branch, Galveston, Texas, USA.

Evaluated Peramivir against pandemic and epidemic influenza virus

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infection with ferret animal model.

Carried out transfection in human cell lines for generation of

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recombinant vaccine influenza virus.

Designed the PCR system for detection of influenza virus strain in

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ferret nasal washes with strain specific as well as universal influenza

A primers.

Established the Reverse-Transcriptase PCR system for detection of

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resistance mutations in virus stock.

Developed of Reverse Transcriptase-PCR assay for detection of

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different strains of Junin virus in animal organs and tissues.

Demonstrated the role of chemokine storm during infection with

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virulent, attenuated and vaccine strains of VEEV in primary

microglia and astrocytes cell culture.

Examined function of different brain cell types and blood/brain

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barrier in chemokine production and protection against lethal VEEV.

Showed the role of γδ T cells subset in brain infection with

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recombinant Sindbis/VEEV vaccine strain virus.

Determined the DNA structure of persistent VEE virus isolated from

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mouse brain and initiated the studies on its role in latent VEEV

infection.

Established primary mouse brain cell culture as an ex vivo model of

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alphavirus infection and a potential alternative to animal in vivo

experiments.

Showed the effectivity of morpholinos against VEEV in mouse

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animal model.

01/2005 – 11/2005 Research Fellow. Department of Obstetrics and Gynecology,

University of Texas Medical Branch, Galveston, Texas, USA.

Performed dual perfusion of human placental lobule

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08/2004 – 01/2005 Research Assistant. Laboratory of Proteomics, Belarussian National

Academy of Science, Minsk, Republic of Belarus.

TEACHING AND VOLUNTEERING EXPERIENCE:

07/2003 – 11/2003 Physical Therapist. Family rehabilitation center for brain-injured

children, Copenhagen, Denmark. IAHP’s Rehabilitation Program for

brain-injured children.

2010 - 2011 High School Science Fair Judge, Galveston Community College.

2010 - 2011 Molecular Biology and Genetics Small-group Discussion Facilitator.

Graduate School of Biomedical Sciences, UTMB, Galveston.

2010 - 2011 Medical Student Summer Research Program Research Poster

Session Judge, School of Medicine, UTMB, Galveston.

2010 -.2010 Molecules, Cells and Tissues PBL Course Facilitator, School of

Medicine, UTMB, Galveston

PROFESSIONAL MEMBERSHIPS:

Member, American Society For Virology (ASV)

Member, American Society For Microbiology (ASM)

AWARDS:

Student Scientific Society Award for a research project “Rehabilitation of patients with

focal scleroderma by influence of low-energy laser on lipid peroxidation”. Belarussian

State Medical University, Minsk, Belarus, 2004.

HOBBIES AND INTERESTS

Singing, Jogging, Public Speaking

SELECTED PUBLICATIONS:

1. Dziuba, N., B. Friedrich, G. Li, J. L. Murray, T. Hodge, W.A. O’Brien, M.

Ferguson. High-throughput Screening and Identification of Cellular Proteins

Required for Replication of Human Immunodeficiency Virus type 1 in Primary

Human Macrophages. Manuscript is in preparation.

2. Li G, Dziuba N, Friedrich B, Murray JL, Ferguson MR. A post-entry role for CD63

in early HIV-1 replication. Virology. 2011 Apr (412(2):315-24)

3. Friedrich, B., G. Li, N. Dziuba, M. Ferguson. Quantitative PCR used to assess HIV-

1 integration and 2-LTR circle formation in human macrophages, peripheral blood

lymphocytes and a CD4+ cell line. Virol J. 2010 Dec (3(7):354)

4. Cabada, M.M., F. Maldonado, K. Mozo, N. Dziuba, E. Gotuzzo. Pre-Travel

Preparation for Cusco, Peru: A Comparison between European and North American

Travelers. Journal of Travel Medicine. 2010. Nov-Dec (17(6):382-6)

5. Chen H.*, N. Dziuba*, B. Friedrich, J. von Lindern, J. L. Murray, D. R. Rojo, T.

Hodge, W. A. O’Brien, M.R. Ferguson. A critical role for CD63 in HIV replication

and infection of macrophages and cell lines. Virology. 2008 Sep 30 (Vol. 379, Issue

2, Pages 191-6). * equal authorship

6. Yun, N., N. Linde, N. Dziuba, M. Zacks, S. Jeanon; S. Jennifer; J. Aronson, O.

Chumakova, H. Lander, C. Peters, S. Paessler. Pathogenesis of XJ and Romero

strain of Junin virus in two strains of guinea pigs. American Journal of Tropical

Medicine& Hygiene. 2008 Aug (Vol. 79, Issue 2, Pages 275-82).

7. Paessler, S., Rijnbrand R., D.A. Stein, H. Ni, N. Yun, N. Dziuba, V. Borisevich, A.

Seregin, Y. Ma; R. Blouch, P. L. Iversen, M.A. Zacks. Inhibition of alphavirus

infection in cell culture and in mice with antisense morpholino oligomers. Virology.

July 2008 (Vol.376, Issue 2, Pages 357-370).

8. Yun, N., N. Linde, M. Zacks, I. Barr, A. Hurt, J. Smith, N. Dziuba, M. Holbrook,

L. Zhang; J. Kilpatrick, C. Arnold, S. Paessler. Injectable peramivir mitigates

disease and promotes survival in ferrets and mice infected with the highly virulent

type A/H5N1 influenza virus. Virology. April 2008 (Vol. 374, Issue 1, Pages 198-

209).

9. Paessler, S., N. Yun, B. Judy, N. Dziuba, M. Zacks, A. Grund, I. Frolov, G.

Campbell, S. Weaver, and D. Estes. Alpha-beta T cells provide protection against

lethal encephalitis in the murine model of VEEV infection. Virology. October 2007

(Vol. 367, Issue 2, Pages 307-323).

10. Veremeichik, A. P., V. E. Butvilovski, N. Dziuba. The effect of low-energy laser

treatment on lipid peroxidation in patients with allergic skin diseases. Materials of

the VI International Conference “Bioantioxidant”, Moscow, Russia. April 16-19,

2002. (Pages 85-87). Article in Russian.

11. Veremeichik, A.P., V.M. Dyuba, N. Dziuba. The effect of low-energy laser therapy

on the lipid peroxidation in the liver of guinea pigs with contact dermatitis. Laser

physics and optic technology. Materials of VI International Conference, Grodno,

Belarus. September 25-29, 2006 (Pages 347-349). Article in Russian.

PUBLISHED ABSTRACTS:

1. Dziuba, N., I. Nekhayeva, T. Nanovskaya, G. Hankins, M.S. Ahmed. Transfer of

metformin across the dually perfused human placental lobule. American Journal of

Obstetrics & Gynecology. December 2005 (Vol. 193, Issue 6, Page S36).

2. Nekhayeva, I., T. Nanovskaya, G. Hankins, N. Dziuba, M.S. Ahmed. The transfer of

glyburide across human placenta obtained from healthy and diabetic patients and the

role of human serum albumin. American Journal of Obstetrics & Gynecology.

December 2005, (Vol.193, Issue 6, Page S92).

ORAL AND POSTER PRESENTATIONS:

1. Dziuba, N., M.R. Ferguson. Identification of cellular factors involved in HIV

replication by siRNA down-regulation in primary human macrophages. Oral

presentation at the Postdoctoral Appreciation Day, Levin Hall, Galveston, TX,

September 24, 2009

2. Dziuba, N., H. Chen, B. Friedrich, W.A. O’Brien, and M.R. Ferguson. CD63 – the

role in HIV infection and replication in macrophages and cell lines. Oral

presentation at the 27th Annual ASV Meeting at Cornell University, Ithaca, New

York, July 12-16, 2008.

3. Dziuba, N., H. Chen, B. Friedrich, W.A. O’Brien, and M.R. Ferguson. CD63 – the

role in HIV infection and replication in macrophages and cell lines. Presented at

IHII/McLaughlin colloquium, Galveston, Texas, April 2, 2008.

4. Ferguson, M.R., B. Friedrich, W.A. O’Brien, and N. Dziuba. The role of tetraspanin

CD63 in HIV entry of macrophages. Presented at FASEB Summer Research

Conference, New Haven, Connecticut, June 22-27, 2008

5. Dziuba, N., K. Bourne, N.Yun, M. Estes, M. Zacks, and S. Paessler. Ex vivo model

of inflammatory response to alphavirus infection. Presented at the 26th Annual ASV

meeting at Oregon State University, Corvalis, Oregon, July 14-18, 2007.

6. Yun, N., H. Ni, B. Judy, N. Dziuba, M. Zacks, A. H. Grund, I. Frolov, G. Campbell,

S. Weaver, M. Estes, and S. Paessler. Alpha-beta T cells provide protection against

lethal encephalitis in the murine model of VEEV infection. Presented at the 26th

Annual ASV meeting at Oregon State University, Corvalis, Oregon, July 14-18,

2007.

7. Paessler, S., N. Yun, N. Linde, N. Dziuba, M. Zacks, J. N. Smith, J. Smith, and M.

Kelley. Pathogenesis of XJ and Romero strain of Junin virus in two strains of

guinea pigs. Presented at the 26th Annual ASV meeting at Oregon State University,

Corvalis, Oregon, July 14-18, 2007.

8. Yun, N., N. Linde, M. Zacks, I. G. Barr, A. C. Hurt, J. N. Smith, N. Dziuba, M.

Holbrook, L. Zhang, J. M. Kilpatrick, C. S. Arnold, and S. Paessler. Injectable

peramivir mitigates disease and promotes survival in ferrets and mice infected with

the highly virulent H5N1 influenza virus isolate A/Vietnam/1203/04. Presented at the

Options for the Control of Influenza VI, Toronto, Ontario, Canada, June 17-23,

2007.

9. Paessler, S., N. E. Yun, H. Ni, B. M. Judy, N. Dziuba, M. A. Zacks, I. Frolov, G. A.

Campbell, S. C. Weaver, and D. M. Estes. Mechanisms of protection from

alphavirus-mediated encephalitis. Presented at the UTMB-MD Anderson

symposium for Immunology/cancer/infectious diseases, Galveston, TX, April 13,

2007.

10. Paessler, S., N. Yun, H. Ni, B. Judy, N. Dziuba, M. Zacks, I. Frolov, G. Campbell,

S. Weaver, and M. Estes. Development of live-attenuated, chimeric alphavirus-

based vaccine against Venezuelan equine encephalitis virus. Presented at the

International Meeting on Emerging Diseases and Surveillance (IMED), Vienna,

Austria, February 23-25, 2007.

11. Zacks*, M., N. Dziuba*, H. Ni, I. Frolov, G. Campbell, N. Yun, S. Weaver, M.

Estes, and S. Paessler. Persistence of attenuated variants of Venezuelan equine

encephalitis virus (VEEV) in the murine brain. Presented at the American Society of

Tropical Medicine and Hygiene 55th Annual Meeting, Atlanta, Georgia, USA,

November 12-16, 2006. * equal authorship

12. Yun, N., N. Linde, M. Zacks, N. Dziuba, J. N. Smith, H. Grund, J. M. Kilpatrick,

and S. Paessler. Peramivir mitigates disease and promotes survival in ferrets and

mice infected with H5N1 influenza virus isolate A/Vietnam/1203/04. Presented at the

American Society of Tropical Medicine and Hygiene 55th Annual Meeting, Atlanta,

Georgia, USA, November 12-16, 2006.

13. Yun, N., D. Estes, N. Dziuba, H. Ni, B. Judy, M.. Zacks, N. Linde and S. Paessler,

Role of CD4+ and CD8+ T cells in protection against lethal encephalitis mediated by

a Sinbis-VEEV chimeric vaccine. Presented at the American Society of Tropical

Medicine and Hygiene 55th Annual Meeting, Atlanta, Georgia, USA, November 12-

16, 2006.

14. Paessler, S., N. Yun, H. Ni, B. Judy, N. Dziuba, M. Zacks, I. Frolov, G. Campbell,

S. Weaver, and M. Estes. Impact of selective lymphoid deficiencies on encephalitis

and virus persistence in the murine brain (VEEV). Presented at the The Changing

Landscape of Vaccine Development: Vaccines for Global Health, Sealy Center for

Vaccine Development, Galveston, Texas, November 8-10, 2006.

15. Arnold, C. S., M. Zacks, N. Dziuba, N. Yun, and N. Linde, et al. Injectable

peramivir is effective in the treatment of highly pathogenic avian influenza

A/Vietnam/1203/04 (H5N1) infections in mouse and ferret models. Presented at the

46th Annual Interscience Conference on Antimicrobial Agents and Chemotherapy,

San Francisco, California, September 30, 2006.

REFERENCES: AVAILABLE UPON REQUEST

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