Atta Ahmad

**** #* ******* *****

Greenville, NC, 27834

Ph: 805-***-****

accfcw@r.postjobfree.com, accfcw@r.postjobfree.com

Present Position (Aug 2013 - ):

Visiting Scientist and Adjunct Assistant Professor

Department of Biology, East Carolina

University, Greenville, NC

Education:

Ph. D (2002)

Molecular and Structural Biology Division,

Central Drug Research Institute, Lucknow,

India Department of Chemistry, HNB Garhwal

University, Garhwal, India

M. Sc. (1996) Department of Chemistry, Aligarh Muslim

University, Aligarh, India

Professional Experience:

2006 -2013

Research Investigator, Biological Chemistry, University of

Michigan, Ann Arbor, MI

2006 -2008

Sr. Scientist, Applied Biotechnology Institute Inc., San Luis

Obispo, CA

2004 - 2006

Post Doctoral Associate, Department of Biology, Massachusetts

Institute of Technology, Cambridge, MA, USA

2001 - 2004

Post Doctoral Fellow, Dept. of Chem & Biochem, University of

California, Santa Cruz, CA

Publications: (* indicates corresponding author)

1. Li X, Srinivasan S, Connarn J, Ahmad A, Young Z, Kabza A, Zuiderweg E,

Sun D, Gestwicki JE. (2013) ACS Med Chem Lett 4, 1042-47

2. Cesa LC, Patury S, Komiyama T, Ahmad A, Zuiderweg ER and Gestwicki JE.

(2013) ACS Chem Biol 8, 1988-97

3. Zuiderweg ERP, Bertelsen EB, Rousaki A, Mayer MP, Gestwicki JE and Ahmad

A (2013) Top Curr Chem 328, 99-153

4. Ahmad A*, Burns CS, Fink AL and Uversky VN (2012) Journal of Biomol

Struct Dyn 29, 825-42

5. Zuiderweg ERP and Ahmad A (2012) Proc Natl Acad Sci USA 109, E735

6. Ahmad A, Bhattacharya A, McDonald RA, Cordes M, Ellington B, Bertelsen

EB and Zuiderweg ERP (2011) Proc Natl Acad Sci USA 108, 18966-71

7. Muzaffar M and Ahmad A* (2011) PLoS ONE 6(11): e27906

8. Ahmad A* (2010) Int J Biol Macromol. 46, 275-79

9. Ahmad A*, Muzaffar M and Ingram VM (2009) Biochim. Biophys. Acta. 1794,

1536-47

10. Munishkina LA, Ahmad A, Fink AL and Uversky VN (2008). Biochemistry 47,

8993-06

11. Hood EE, Love R, Lane J, Bray J, Clough R, Pappu K, Drees C, Hood KR,

Yoon S, Ahmad A, Howard JA (2007) Plant Biotechnol J. 5, 709-19

12. Hong DP, Ahmad A and Fink AL (2006) Biochemistry 45, 9342-53

13. Ahmad A, Uversky VN, Hong D and Fink AL (2005) J Biol. Chem. 280, 42669-

75

14. Ahmad A, Millet IS, Doniach S, Uversky VN and Fink AL (2004) J Biol.

Chem. 279, 14999-13

15. Ahmad A, Millet IS, Doniach S, Uversky VN and Fink AL (2003)

Biochemistry 42, 11404-16

16. Akhtar MS, Ahmad A and Bhakuni V (2002) Biochemistry 41, 3819-27

17. Akhtar MS, Ahmad A and Bhakuni V (2002) Biochemistry 41, 7142-49

18. Ahmad A, Akhtar MS and Bhakuni V (2001) Biochemistry 40, 1945-55

19. Ahmad A, Madhusudanan KP and Bhakuni V (2000) Biochim. Biophys. Acta.

1480, 201-10

20. Ali V, Prakash K, Kulkarni S, Ahmad A, Madhu KP and Bhakuni V (1999)

Biochemistry 38, 13635-42

Book Chapter:

1. Zuiderweg ERP, Bertelsen EB, Rousaki A, Mayer MP, Gestwicki JE and Ahmad

A (2012) Allostery in the Hsp70 chaperone proteins. Series: Topics in

Current Chemistry; Volume 328; Page 99-153.

Invited Talks/Lectures:

1. Institute of Environmental Health Sciences, Wayne State University,

Detroit, MI August 2013

2. Dophen Biomed, Sacramento, California, June 2013

3. Midwest Chaperone Conference, Chicago, January 2010

4. Applied Biotechnology Institute, Cal Ploy, San Luis Obispo, California,

July 2006

5. Adlyfe Pharmaceuticals, Baltimore, Maryland, March 2006

6. University of Texas Medical Branch, Galveston, Department of Neurology,

March 2006

7. ExSar Therapies, Princeton, New Jersey, April 2005

8. Massachusetts Institute of Technology, Biology Department, August 2004

9. University of Massachusetts Medical School, Worcester, Dept of Biochem

and Mol Pharm, January 2004

10. Tufts University, Department of Physiology, December 2003

11. University of Wisconsin, Madison, Department of Chemistry, November

2003

12. University of Virginia, Department of Biochemistry and Molecular

Genetics, August 2003

13. Dr. C R Krishnamurthy memorial oration and young scientist awards CDRI

Lucknow, India March 2000

Presentations:

1. Cesa LC, Patury S, Komiyama T, Ahmad A, Zuiderweg ERP, Gestwicki JE

(January 19, 2013) 19th Annual Midwest Stress Response and Molecular

Chaperone Meeting, Northwestern University, Chicago, IL USA

2. Zuiderweg EPR, Ahmad A, Rousaki A, Gestwicki JE (May 1-5, 2012) Cold

spring harbor, New York USA

3. Ahmad A, Bhattacharya A, Macdonald R, Bertelsen E, Zuiderweg E (July 25-

30, 2010) Protein Folding in the Cell?; The FASEB Summer Research

Conference, Saxtons River, Vermont, USA

4. Bertelsen E, Bhattacharya A, McDonald R, Ahmad A, Zuiderweg E (January

16, 2010) 15th Annual Midwest Stress Response and Molecular Chaperone

Meeting, Northwestern University, Chicago, IL USA

5. Ahmad A and Howard JA (Feb 2-6, 2008) Biophysical society Los Angeles,

California, USA

6. Ahmad A, Blanchard B, Ingram VM (Jan 2006) Massachusetts General

Hospital, Boston MA, USA

7. Ahmad A, Blanchard B, Ingram VM (Nov 12-16, 2005) 35th Meeting, Society

for Neurosciences, Washington DC, USA

8. Ingram VM, Blanchard B, Ahmad A (Sept 7-11, 2005) Strategies for

Engineered negligible senescence (SENS) IInd conference Queens College

Cambridge UK

9. Ahmad A and Ingram VM (Aug 28- Sep 1, 2005) Small molecules DAPH, decoy

peptides and their analogs may help rescuing the toxic effect of A beta 1-

42 in Alzheimer's disease, ACS Washington DC,

10. Ahmad A, Burns CS and Fink AL (March 1-5, 2003) Biophysical society

San Antonio, Texas, USA

11. Ahmad A, Millet IS, Doniach S, Fink AL (August 17-21, 2002) 16th

Symposium of the Protein Society San Diego, California, USA

12. Ahmad A, Kulkarni S, Madhusudanan KP and Bhakuni V (September 19-24,

1999) XIII International Biophysics Conference, New Delhi, India

13. Bhakuni V, Kulkarni S, Ahmad A and Prakash K (September 19-24, 1999)

XIII International Biophysics Conference, New Delhi, India

Funding:

Title: "Examining role of chaperones in protein quality control and its

relation with diabetes." Agency: MDRC Michigan - Status: Awarded - $69,976.

Awards and Honors:

1. Awarded Senior Research Fellow (SRF) (2000) by CSIR, Ministry of

Education, Government of India

2. Qualified Graduate Aptitude test in Engineering (GATE) in Chemistry

(1997) IIT, India

3. Awarded post-graduate merit scholarship, Department of Chemistry, AMU,

Aligarh, India (1994-96)

4. Member youth organizing Committee, International Conference on molecular

association (1996), Department of Chemistry, AMU, Aligarh, India

Memberships:

(1) Protein Society (USA); (2) Sigma Xi; (3) Biophysical Society (USA)

Thesis supervised:

1. Janelle Crane: Undergraduate thesis "Fibrillation of ?-synuclein"

Department of Chemistry and Biochemistry, University of California, Santa

Cruz, California, USA

2. Michael Downing: Undergraduate thesis "Effect of SDS on fibrillation

of A? peptides involved in Alzheimer's disease" Department of Biology,

Massachusetts Institute of Technology, Cambridge, MA, USA.

Teaching Experience:

1. Teaching instructor: Advance undergraduate course (7.346) in

Biology: Feb 2006 - May 2006: Department of Biology, Massachusetts

Institute of Technology (MIT), MA, USA. Prof. Bob Horvitz (Nobel Laureate

Physiology or Medicine 2002) Director, instructor/course-screening process.

Selected Teaching Instructor for 2007 semester - did not participate due to

conflicting schedules.

2. Teaching Instructor: Graduate course (BIOLCHEM 530 - Structural

Biology in Solution); Teaching biophysical techniques; Department of

Biological Chemistry, University of Michigan, Ann Arbor, 2009-Current

Editorial Board member:

(1) The Scientific World Journal; (2) International Scholarly Research

Network; (3) Alzheimer's & Dementia

Reviewed/Reviewer for:

(1) Alzheimer's Association; (2) Current Neuropharmacology; (3) Current

Proteomics; (4) Cure Parkinson Trust, London, UK; (5) Biochemistry Research

International; (6) ACS Chemical Neuroscience; (7) PLoS ONE

Synopsis:

I have more than 16 years of hands on experience with protein biological

and biophysical chemistry and am associated with chemical biology for two

years. (Please see papers published in corresponding year)

> I was part of one of the groups that applied mass spectroscopy to study

the protein-folding problem (1998-99).

> I explored conditions for industrially important enzyme Glucose oxidase

to make it 10 C more stable to temperature and 50% more stable towards

chemical denaturation, using Fluorescence, CD, FPLC, DSC, enzyme assays,

UV-visible Absorbance, proteolysis and electrophoresis, (2001-2002).

> I conceptualized a methodology to study stability and aggregation

propensity of pharmacologically important protein insulin using H/D

exchange Mass spectroscopy, SAXS, HPLC, ATR-FTIR, DLS, thus, accumulating

and analyzing biochemical and biophysical data in extensive detail which

resulted in publishing 6 articles as first, collaborating or

corresponding author that have been cited more than 300 times, (2003-13).

> I showed copper at ?M concentrations can aggravate Parkinson's disease

causing protein ?-synuclein aggregation by SEC, IEX, RP or affinity

chromatography, EPR and ITC (2012).

> I established calcium at physiological conditions could induce amyloid

formation of A-beta involved in Alzheimer's, (2009).

> For pharmacological intervention of neurodegenerative or other diseases I

proposed to harness the potential of physiological protein quality

control machinery - chaperones, and am working on it (2010).

> I helped solve the structure of DnaK/DnaJ complex by NMR method, (TROSY,

HSQC, MTSL protein labeling) using Site directed mutagenesis (2011).

> For developing a chemical pharmacophore against cancer, I worked with

groups at Ann Arbor on screening of chemical libraries and reported two

molecules of interest recently, (2013).

> My pilot-grant application of using chaperones to interfere in protein

aggregation relevant to diabetes was awarded recently ($45K) from MRDC

Michigan.

> I worked for two years in a biotechnology company on characterizing

proteins expressed in genetically modified plants (corn) for use in

industries related to bleaching (manganese peroxidase - as an alternative

for green chemistry), Food industry (monellin family as protein

sweetener); Biofuels industry (cellulose - for cellulose hydrolysis-

ethanol) and expression of oral vaccines of protein-origin in plants for

diseases like HIV and Hepatitis C using immunochemical methods like ELISA

and western blots (2007).

Technical research experience (Briefly listed)

Proteins/enzymes: Protein isolation and purification using (SEC, IEX, RP

and affinity chromatography HPLC/FPLC). One and 2-dimensional

electrophoresis, Isoelectric-focusing, Chemical and Radiolabelling and

proteolysis of proteins, enzyme assays, Electron Micrography (EM).

Immuno-Chemical: ELISA assays; Antibody purification and Western blot

Spectroscopy: UV-visible Absorbance, Circular Dichroism (CD), Metal

(Copper) binding to proteins by X-band Electron Paramagnetic Resonance

(EPR), Characterization of secondary structure of proteins by ATR-FTIR,

Characterization of protein conformations by H/D exchange by Mass

Spectrometry, Small Angel X-ray Scattering (SAXS), Fluorescence (intrinsic

and using dyes), Fluorometric/spectrophotometric studies by Multi well

plate readers.

Calorimetry: Isothermal Titration Calorimetry (ITC), Differential Scanning

Calorimetry (DSC)

DNA & RNA: DNA (plasmid) isolation and purification, Agarose

electrophoresis and Site directed mutagenesis

Peptide Chemistry: Edman degradation and Solid phase synthesis of peptide

fragments, TLC.

Crystallography: Hanging/sitting drop method of crystal production.

Cell culture: Maintenance, transfection and related work cos7, IMR32 cell

line, Cell viability assays (MTT)

NMR: Studying - current; Protein peak assignment, TROSY, HSQC

Visa status: US permanent resident - Green card

References: (on request)[pic]

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