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Medical Director

Location:
Phoenix, AZ
Posted:
January 31, 2014

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Resume:

Enoc Juan Hollemweguer, Ph.D., SI(ASCP), ABMLI

***** *. *** **** **.

Sun City West, AZ 85375

623-***-****

accf54@r.postjobfree.com

CURRICULUM VITAE

Education

University of South Dakota, Vermillion, S.D.

1978

Doctorate in Microbiology, Biochemistry Minor

Morningside College, Sioux City, IA

1970

B.S. Biology, Chemistry Minor

Honors

National Jewish Center for Immunology Research Fellowship

1980

Mayo Foundation Research Fellowship

1978-1980

Lucille B. Wendt Scholarship

1972

Sister City Exchange Student Scholarship

1966-1970

Credentials

Certified and Registered Specialist in Immunology

1982-Present

American Society for Clinical Pathologists

February 1982; Certificate No. 33

Certified as Diplomate 1985-

Present

American Board of Medical Laboratory Immunology

April 1985; Certificate No. 79

Laboratory Director License 1989-

1994

Diagnostic Immunology, State of Tennessee

Laboratory Director License 1989-

1995

Diagnostic Immunology/Cellular Immunology, State of New York

Laboratory Director License 1989-

1995

Diagnostic Immunology/Cellular Immunology, City of New York

Laboratory Director License 1981-

1990

State of Florida

Membership in Professional Societies

Association of Medical Laboratory Immunologists

1988-Present

American Society of Clinical Pathologists

1982-Present

Colorado Association for Continuing Medical Laboratory Education

1980

Association of Minnesota Immunologists

1978-1980

The Society of Sigma Xi

1977-1980

American Society for Microbiology

1973-1981

Professional Experience

Medical Laboratory Scientist Dec

2009 - Present

Sonora Quest Laboratories/Laboratory Sciences of Arizona

Banner Thunderbird Medical Center

Hematology

Technical Coordinator Evening Shift May 2009 -

Nov 2009

Sonora Quest Laboratories/Laboratory Sciences of Arizona

Banner Del E Webb Medical Center

Hematology Technical Coordinator Dec 2005 -

2009

Sonora Quest Laboratories/Laboratory Sciences of Arizona

Banner Thunderbird Medical Center

Hematology Section Lead Tech

Laboratory Technologist June

2005 - Dec - 2005

Hemex Laboratories, Phoenix, AZ

. Flow Cytometry technologist

. Assay Development

Program Manager, Clinical Immunology Research

1994-2005

BD-BIOSCIENCES PHARMINGEN, San Diego, CA

. New Product Planning, Marketing, Technical Service and Customer

Support.

. Develop technical & educational literature.

. Product Management and Coordination of Product Development.

. Quality Control and Manufacturing of monoclonal antibodies.

Biological Safety Officer

1996-2005

BD- BIOSCIENCES PHARMINGEN

. Member of Safety Committee.

. In charge of Bloodborne Pathogen training.

. Review requests to bring in cancer cell lines to cell bank.

Technical Director & Continuing Education Coordinator

1993-1994

CYTOMETRY ASSOCIATES, INC., Brentwood, TN

. Flowcytometric data interpretation of leukemia/lymphoma cases

for reporting to physicians.

. Performed flowcytometric analysis.

. Platelet Antibody & DNA Ploidy data analysis.

. Progenitor cell (CD34+) analysis.

. Obtained good knowledge of molecular analysis (T & B cell gene

re-arrangement, bcr/abl, bcl-2 southern blotting).

. Assist in the operation of laboratory, train nine laboratory

personnel.

. Provide consultation services to physicians.

Director, Flow Cytometry

1989 - 1993

National Reference Laboratory

NATIONAL HEALTH LABORATORIES, INC., Nashville, TN

. Management of Flow Cytometry section, budgeting, development

of new tests, train ten technologists.

. Performed flowcytometric analysis of HIV samples.

. Review of patient data (T-helper/T-suppressor analysis in HIV

cases).

. Provide consultation services to physicians.

Director, Immunology/Flow Cytometry

1989-1991

National Reference Laboratory

NATIONAL HEALTH LABORATORIES, INC., Nashville, TN

. Development of both, Immunology and Flow Cytometry sections.

. Management of Immunology and Flow Cytometry, budgeting, test

developments, hiring and training personnel.

. Review patient data (Autoimmune disorders, Complement function

assays,

HLA typing, using various immunoserolgical methods).

. Provide consultation services to physicians.

Director, Immunology Laboratory

1981-1989

Department of Pathology

ORLANDO REGIONAL MEDICAL CENTER, Orlando, FL

. Design and development of immunology laboratory.

. Management of immunology, budgeting, new test development, train

personnel.

. Assist technologists in performance of immunoassays.

. Review patient data and provide consultation to physicians.

Member, AIDS Task Force

1987-1989

ORLANDO REGIONAL MEDICAL CENTER, Orlando, FL

. Cooperated with Infection Control and Medical Education

Departments.

. Provided lectures and seminars for hospital personnel.

Member, Laboratory Safety Committee

1986-1989

ORLANDO REGIONAL MEDICAL CENTER, Orlando, FL

. Assisted in the design and implementation of procedures to

protect the personnel against Hepatitis B and HIV contaminated

material.

. Provided seminars for laboratory personnel

Member, Education Committee 1981 -

1989

School of Medical Technology

ORLANDO REGIONAL MEDICAL CENTER, Orlando, FL

. Taught Clinical Immunology to medical technology students.

. Developed complete Clinical Immunology course for laboratory

personnel.

. Developed workshops and training sessions for medical technology

students and pathology residents rotating through immunology

lab.

Research Fellow-Tumor Immunology 1980 -

1981

Department of Medicine

NATIONAL JEWISH CENTER FOR IMMUNOLOGY, Denver, CO

. Developed RIA methods for the study of tumor associated

antigens.

. Antibody purification through cellular immunoadsorbtion.

. Tumor associated antigen purification through affinity

chromatography.

. Tissue culture of tumor cells to the production of polyclonal

and monoclonal antibodies.

Research Fellow-Tumor Immunology 1977 -

1980

Microbiology Research Laboratory

MAYO CLINIC, Rochester, MN

. Study of cell mediated immunity to avian retroviruses.

. Tissue culture of infected fibroblasts for virus propagation.

. Adapted Leukocyte Adherence Inhibition (LAI) to avian retrovirus

system.

. Conducted cellular cytotoxicity assays on infected cells.

. Conducted lymphocyte blastogenesis assays to avian retrovirus

antigens.

Graduate Student-Research Assistant

1973-1977

Department of Microbiology

SCHOOL OF MEDICINE, UNIVERSITY OF SOUTH DAKOTA, Vermillion, S.D.

. Assisted in departmental research on mycoplasmas

. Conducted graduate research on Acholeplasma axanthum.

. Studied humoral and cellular immune response to mycoplasma

derived

membrane antigens.

. Underwent tissue culture and virus propagation training.

. Assisted postdoctoral fellows with their research on

mycoplasmas.

Graduate Student-Teaching Assistant

1972-1973

Department of Microbiology

SCHOOL OF MEDICINE, UNIVERSITY OF SOUTH DAKOTA,Vermillion, S.D.

. Taught Clinical Microbiology/Serology to Medical Technology,

Dental Hygiene and Nursing Students.

. Assisted in lectures to medical students.

Laboratory Technician 1970 -

1977

ST. LUKE'S REGIONAL MEDICAL CENTER, Sioux City, IA

. Training in bacteriology, hematology, serology, blood banking,

coagulation, chemistry, and phlebotomy.

. Worked full time first year and part time while pursuing Ph.D.

Teaching Experience

Adjunct Faculty May 2005 -

July 2005

Biology - Microbiology Instructor

Estrella Mountain Community College, Avondale, AZ

Adjunct Faculty/Consultant/Trainer

1999-2005

Biotechnology Training Program & General Biology

Miramar College, San Diego, CA

Guest Lecturer 1999-

2001

Biotechnology Training Program

Mesa College, San Diego, CA

Adjunct Faculty 1998-

2001

Biotechnology Training Program

San Diego City College, San Diego, CA

Immunology Lecturer 1983-

1986

Immunology, Immunodiagnostics

Central Florida Blood Bank, Orlando, FL

Faculty Member 1981-

1989

School of Medical Technology

Orlando Regional Medical Center, Orlando, FL

Faculty Member 1981-

1989

Pathology Residency Program

Lecturer 1981-

1989

Medical Education: Internal Medicine

Orlando Regional Medical Center, Orlando, FL

Continuing Education 1980 -

Present

. Attended symposia, courses, immunology meetings to maintain

current with new immunoassay and molecular biology technologies.

. Attended autoimmunity, immunodeficiency, tumor immunology and

molecular biology technology seminars.

. Attended various seminars on HIV immunopathology.

. Attended monoclonal antibody development courses.

. Attended instrument training courses, flowcytometers.

. Maintained current knowledge in diagnostic immunology through

review courses.

Publications, Presentations and Abstracts

An energy source for Thermoplasma acidophilum. 1973. Enoc J. Hollemweguer

ASM News. (North Central Branch ASM, Iowa City, IA, October 12, 1973). ASM

News. 39 (No. 12). 801.

Mycoplasma membrane derived antigens and lymphocyte transformation. 1975.

Enoc J. Hollemweguer. ASM News. (North Central Branch ASM, Madison, WI.,

October 16, 1975). ASM News, 41

Characterization of the immune response to lipopolysaccharide of

Acholeplasma axanthum. 1976. R. J. Lynn and E. J. Hollemweguer. Abstracts

of Annual Meeting of the ASM (1976), p. 76 (ASM, Atlantic City, N.J., May 2-

7, 1976).

Modulation of the immune response to bovine serum albumin (BSA) by

lipopolysaccharide of Acholeplasma axanthum. 1977. E. J. Hollemweguer and

R. J.Lynn. ASM News (North Central Branch ASM, Minneapolis, MN, October 29,

1977) ASM News, 44 (No. 2), 76.

An investigation of the immune response of rabbits to Acholeplasma axanthum

lipopolysaccharide. Jan 1978. Enoc J. Hollemweguer. Doctoral

Dissertation. University of South Dakota., Vermillion, S.D.

Humoral response of rabbits to acholeplasmal lipoglycans. 1980. R. J.

Lynn, P. Aneccino Guidici, E. J. Hollemweguer and P. F. Smith. Infec.

Immun. 29:926-933

Leukocyte Adherence Inhibition (LAI) reactivity to avian RNA tumor virus

induced antigens. E. J. Hollemweguer and P. Meyers. Abstracts of Annual

Meeting of the ASM (1980). p. 73 (ASM, Miami, FL., May 11-17, 1980).

Hepatitis Profiling: A Guide to Test Selection. Enoc J. Hollemweguer.

Laboratory Management 26(4):30-34, 1988.

Anti-Nuclear Antibodies: Comparison Study of Enzymeimmunoassay and

Immunofluorescence. Enoc J. Hollemweguer, Greg Mitchell and Martha Lee.

Abstract of 3rd Annual Meeting of the Association of Medical Laboratory

Immunologists, Washington, D.C., August 2-6, 1990.

HLA-B27 Determination by Immunofluorescence as Compared with Lymphocyte

Microcytotoxicity. Enoc J. Hollemweguer, Teri Jackson, Diane Hester and

Janice Arnett. Abstract of 3rd Annual Meeting of the Association of Medical

Laboratory Immunologists, Washington, D.C., August 2-6, 1990.

Autoantibodies to Extractable Nuclear Antigens, a Comparison Study:

Enzymeimmunoassay and Double Immunodiffusion. Enoc J. Hollemweguer, Teri

Jackson, Rhonda Harris and Janice Arnett. Abstract of 3rd Annual Meeting of

the Association of Medical Laboratory Immunologists, Washington, D.C.,

August 2-6, 1990.

Study of T-cytotoxic cells in Normal and HIV infected individuals using the

S6F1 Marker. Enoc J. Hollemweguer, Karen Johnson and Loretta DeWald.

Abstract of 3rd Annual Meeting of the Association of Medical Laboratory

Immunologists, Washington, D.C., August 2-6, 1990.

Yu. J.F., X.F Yang, E. Nguyen, G.X. Lin, E. Hollemweguer, and Z. Chen.

VIIth HLDA workshop cytokine receptor section: flow cytometric analysis of

65 antibodies on 11 different target cells. In: Mason D, Andre P,

Bensussan A, et al, eds. Leucocyte Typing VII: White Cell Differentiation

Antigens. New York, NY: Oxford University Press 2002: 275 - 278.

Yu, J.F., X.F. Yang, L.R. Shi, B.C. Sang, E. Nguyen, E. Hollemweguer, T.

Ward and Z. Chen. Clone D2-1173 is a novel monoclonal antibody against

human CD30L (CD153). In: Mason D, Andre P, Bensussan A, et al, eds.

Leucocyte Typing VII: White Cell Differentiation Antigens. New York, NY:

Oxford University Press 2002: 712-714.

Yu, J.F., X.F. Yang, L.R. Shi, B.C. Sang, E. Nguyen, E. Hollemweguer, T

Ward and Z. Chen. Generation and characterization of two novel monoclonal

antibodies against human 4-1BB ligand. In: Mason D, Andre P, Bensussan A,

et al, eds. Leucocyte Typing VII: White Cell Differentiation Antigens. New

York, NY: Oxford University Press 2002: 714 - 716.

Wu, X.W., E. Hollemweguer, and Z. Chen. SN2: a monoclonal antibody to CD165

induces platelet activation. In: Mason D, Andre P, Bensussan A, et al,

eds. Leucocyte Typing VII: White Cell Differentiation Antigens. New York,

NY: Oxford University Press 2002: 556 - 558.

Wu, X.W., E. Nguyen, E. Hollemweguer, and Z. Chen. Flow cytometric analysis

and functional studies of four monoclonal antibodies from the platelet

blind panel. In: Mason D, Andre P, Bensussan A, et al, eds. Leucocyte

Typing VII: White Cell Differentiation Antigens. New York, NY: Oxford

University Press 2002: 546-548.

Lin, G.X., X.F. Yang, E. Hollemweguer, J.F. Yu, L. Li, X.W. Wu, and Z.

Chen. Cross reactivity of CD antibodies on eight animal species. In:

Mason D, Andre P, Bensussan A, et al, eds. Leucocyte Typing VII: White Cell

Differentiation Antigens. New York, NY: Oxford University Press 2002: 519 -

524.

Effect of monoclonal antibodies to platelet associated antigens on platelet

function. Xiao-wei Wu, Enoc J. Hollemweguer, 13th annual meeting of the

Association of Medical Laboratory Immunology, St. Petersburg, FL, July,

2000.

Monoclonal antibodies raised to human cells-specificity for pig leukocytes.

2001. Haverson, K., Bailey, M., Stokes, C.R., Simon, A., Le fluffy, L.,

Banfield, G., Chen, Z., Hollemweguer, E., Ledbetter, J.A., Vet. Immunol.

Immunopathol. 80: 175-186

Summary of workshop findings for porcine T-lymphocyte-specific monoclonal

antibodies. 2001. Saalmuller, A., Kuebart, G., Hollemweguer, E., Chen, Z.,

Nielsen, J., Zuckerman, F., Haverson, K. Vet. Immunol. Immunopathol. 80: 35-

52.

References

David Ernst, Ph.D.

Associate Director Research & Development

BD Biosciences

10975 Torreyana Rd.

San Diego, CA 92121

858-***-****

accf54@r.postjobfree.com

David Singer, Ph.D.

Biology Dept.

San Diego City College

1313 Park Boulevard

San Diego, CA 92101

619-***-****

accf54@r.postjobfree.com

Sandra Slivka, Ph.D.

Biology Dept.

Miramar College

10440 Black Mountain Rd.

San Diego, CA 92126

619-***-****

accf54@r.postjobfree.com

Daniel Trubovitz, Ph.D.

Biology Dept.

Miramar College

10440 Black Mountain Rd.

San Diego, CA 92126

619-***-****

accf54@r.postjobfree.com

Mr. Robert Fremland

Chemistry Dept.

San Diego Mesa College

7250 Mesa College Dr.

San Diego, CA 92111

619-***-****

accf54@r.postjobfree.com



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