Medical Director
Resume:
Enoc Juan Hollemweguer, Ph.D., SI(ASCP), ABMLI
Sun City West, AZ 85375
accf54@r.postjobfree.com
CURRICULUM VITAE
Education
University of South Dakota, Vermillion, S.D.
1978
Doctorate in Microbiology, Biochemistry Minor
Morningside College, Sioux City, IA
1970
B.S. Biology, Chemistry Minor
Honors
National Jewish Center for Immunology Research Fellowship
1980
Mayo Foundation Research Fellowship
1978-1980
Lucille B. Wendt Scholarship
1972
Sister City Exchange Student Scholarship
1966-1970
Credentials
Certified and Registered Specialist in Immunology
1982-Present
American Society for Clinical Pathologists
February 1982; Certificate No. 33
Certified as Diplomate 1985-
Present
American Board of Medical Laboratory Immunology
April 1985; Certificate No. 79
Laboratory Director License 1989-
1994
Diagnostic Immunology, State of Tennessee
Laboratory Director License 1989-
1995
Diagnostic Immunology/Cellular Immunology, State of New York
Laboratory Director License 1989-
1995
Diagnostic Immunology/Cellular Immunology, City of New York
Laboratory Director License 1981-
1990
State of Florida
Membership in Professional Societies
Association of Medical Laboratory Immunologists
1988-Present
American Society of Clinical Pathologists
1982-Present
Colorado Association for Continuing Medical Laboratory Education
1980
Association of Minnesota Immunologists
1978-1980
The Society of Sigma Xi
1977-1980
American Society for Microbiology
1973-1981
Professional Experience
Medical Laboratory Scientist Dec
2009 - Present
Sonora Quest Laboratories/Laboratory Sciences of Arizona
Banner Thunderbird Medical Center
Hematology
Technical Coordinator Evening Shift May 2009 -
Nov 2009
Sonora Quest Laboratories/Laboratory Sciences of Arizona
Banner Del E Webb Medical Center
Hematology Technical Coordinator Dec 2005 -
2009
Sonora Quest Laboratories/Laboratory Sciences of Arizona
Banner Thunderbird Medical Center
Hematology Section Lead Tech
Laboratory Technologist June
2005 - Dec - 2005
Hemex Laboratories, Phoenix, AZ
. Flow Cytometry technologist
. Assay Development
Program Manager, Clinical Immunology Research
1994-2005
BD-BIOSCIENCES PHARMINGEN, San Diego, CA
. New Product Planning, Marketing, Technical Service and Customer
Support.
. Develop technical & educational literature.
. Product Management and Coordination of Product Development.
. Quality Control and Manufacturing of monoclonal antibodies.
Biological Safety Officer
1996-2005
BD- BIOSCIENCES PHARMINGEN
. Member of Safety Committee.
. In charge of Bloodborne Pathogen training.
. Review requests to bring in cancer cell lines to cell bank.
Technical Director & Continuing Education Coordinator
1993-1994
CYTOMETRY ASSOCIATES, INC., Brentwood, TN
. Flowcytometric data interpretation of leukemia/lymphoma cases
for reporting to physicians.
. Performed flowcytometric analysis.
. Platelet Antibody & DNA Ploidy data analysis.
. Progenitor cell (CD34+) analysis.
. Obtained good knowledge of molecular analysis (T & B cell gene
re-arrangement, bcr/abl, bcl-2 southern blotting).
. Assist in the operation of laboratory, train nine laboratory
personnel.
. Provide consultation services to physicians.
Director, Flow Cytometry
1989 - 1993
National Reference Laboratory
NATIONAL HEALTH LABORATORIES, INC., Nashville, TN
. Management of Flow Cytometry section, budgeting, development
of new tests, train ten technologists.
. Performed flowcytometric analysis of HIV samples.
. Review of patient data (T-helper/T-suppressor analysis in HIV
cases).
. Provide consultation services to physicians.
Director, Immunology/Flow Cytometry
1989-1991
National Reference Laboratory
NATIONAL HEALTH LABORATORIES, INC., Nashville, TN
. Development of both, Immunology and Flow Cytometry sections.
. Management of Immunology and Flow Cytometry, budgeting, test
developments, hiring and training personnel.
. Review patient data (Autoimmune disorders, Complement function
assays,
HLA typing, using various immunoserolgical methods).
. Provide consultation services to physicians.
Director, Immunology Laboratory
1981-1989
Department of Pathology
ORLANDO REGIONAL MEDICAL CENTER, Orlando, FL
. Design and development of immunology laboratory.
. Management of immunology, budgeting, new test development, train
personnel.
. Assist technologists in performance of immunoassays.
. Review patient data and provide consultation to physicians.
Member, AIDS Task Force
1987-1989
ORLANDO REGIONAL MEDICAL CENTER, Orlando, FL
. Cooperated with Infection Control and Medical Education
Departments.
. Provided lectures and seminars for hospital personnel.
Member, Laboratory Safety Committee
1986-1989
ORLANDO REGIONAL MEDICAL CENTER, Orlando, FL
. Assisted in the design and implementation of procedures to
protect the personnel against Hepatitis B and HIV contaminated
material.
. Provided seminars for laboratory personnel
Member, Education Committee 1981 -
1989
School of Medical Technology
ORLANDO REGIONAL MEDICAL CENTER, Orlando, FL
. Taught Clinical Immunology to medical technology students.
. Developed complete Clinical Immunology course for laboratory
personnel.
. Developed workshops and training sessions for medical technology
students and pathology residents rotating through immunology
lab.
Research Fellow-Tumor Immunology 1980 -
1981
Department of Medicine
NATIONAL JEWISH CENTER FOR IMMUNOLOGY, Denver, CO
. Developed RIA methods for the study of tumor associated
antigens.
. Antibody purification through cellular immunoadsorbtion.
. Tumor associated antigen purification through affinity
chromatography.
. Tissue culture of tumor cells to the production of polyclonal
and monoclonal antibodies.
Research Fellow-Tumor Immunology 1977 -
1980
Microbiology Research Laboratory
MAYO CLINIC, Rochester, MN
. Study of cell mediated immunity to avian retroviruses.
. Tissue culture of infected fibroblasts for virus propagation.
. Adapted Leukocyte Adherence Inhibition (LAI) to avian retrovirus
system.
. Conducted cellular cytotoxicity assays on infected cells.
. Conducted lymphocyte blastogenesis assays to avian retrovirus
antigens.
Graduate Student-Research Assistant
1973-1977
Department of Microbiology
SCHOOL OF MEDICINE, UNIVERSITY OF SOUTH DAKOTA, Vermillion, S.D.
. Assisted in departmental research on mycoplasmas
. Conducted graduate research on Acholeplasma axanthum.
. Studied humoral and cellular immune response to mycoplasma
derived
membrane antigens.
. Underwent tissue culture and virus propagation training.
. Assisted postdoctoral fellows with their research on
mycoplasmas.
Graduate Student-Teaching Assistant
1972-1973
Department of Microbiology
SCHOOL OF MEDICINE, UNIVERSITY OF SOUTH DAKOTA,Vermillion, S.D.
. Taught Clinical Microbiology/Serology to Medical Technology,
Dental Hygiene and Nursing Students.
. Assisted in lectures to medical students.
Laboratory Technician 1970 -
1977
ST. LUKE'S REGIONAL MEDICAL CENTER, Sioux City, IA
. Training in bacteriology, hematology, serology, blood banking,
coagulation, chemistry, and phlebotomy.
. Worked full time first year and part time while pursuing Ph.D.
Teaching Experience
Adjunct Faculty May 2005 -
July 2005
Biology - Microbiology Instructor
Estrella Mountain Community College, Avondale, AZ
Adjunct Faculty/Consultant/Trainer
1999-2005
Biotechnology Training Program & General Biology
Miramar College, San Diego, CA
Guest Lecturer 1999-
2001
Biotechnology Training Program
Mesa College, San Diego, CA
Adjunct Faculty 1998-
2001
Biotechnology Training Program
San Diego City College, San Diego, CA
Immunology Lecturer 1983-
1986
Immunology, Immunodiagnostics
Central Florida Blood Bank, Orlando, FL
Faculty Member 1981-
1989
School of Medical Technology
Orlando Regional Medical Center, Orlando, FL
Faculty Member 1981-
1989
Pathology Residency Program
Lecturer 1981-
1989
Medical Education: Internal Medicine
Orlando Regional Medical Center, Orlando, FL
Continuing Education 1980 -
Present
. Attended symposia, courses, immunology meetings to maintain
current with new immunoassay and molecular biology technologies.
. Attended autoimmunity, immunodeficiency, tumor immunology and
molecular biology technology seminars.
. Attended various seminars on HIV immunopathology.
. Attended monoclonal antibody development courses.
. Attended instrument training courses, flowcytometers.
. Maintained current knowledge in diagnostic immunology through
review courses.
Publications, Presentations and Abstracts
An energy source for Thermoplasma acidophilum. 1973. Enoc J. Hollemweguer
ASM News. (North Central Branch ASM, Iowa City, IA, October 12, 1973). ASM
News. 39 (No. 12). 801.
Mycoplasma membrane derived antigens and lymphocyte transformation. 1975.
Enoc J. Hollemweguer. ASM News. (North Central Branch ASM, Madison, WI.,
October 16, 1975). ASM News, 41
Characterization of the immune response to lipopolysaccharide of
Acholeplasma axanthum. 1976. R. J. Lynn and E. J. Hollemweguer. Abstracts
of Annual Meeting of the ASM (1976), p. 76 (ASM, Atlantic City, N.J., May 2-
7, 1976).
Modulation of the immune response to bovine serum albumin (BSA) by
lipopolysaccharide of Acholeplasma axanthum. 1977. E. J. Hollemweguer and
R. J.Lynn. ASM News (North Central Branch ASM, Minneapolis, MN, October 29,
1977) ASM News, 44 (No. 2), 76.
An investigation of the immune response of rabbits to Acholeplasma axanthum
lipopolysaccharide. Jan 1978. Enoc J. Hollemweguer. Doctoral
Dissertation. University of South Dakota., Vermillion, S.D.
Humoral response of rabbits to acholeplasmal lipoglycans. 1980. R. J.
Lynn, P. Aneccino Guidici, E. J. Hollemweguer and P. F. Smith. Infec.
Immun. 29:926-933
Leukocyte Adherence Inhibition (LAI) reactivity to avian RNA tumor virus
induced antigens. E. J. Hollemweguer and P. Meyers. Abstracts of Annual
Meeting of the ASM (1980). p. 73 (ASM, Miami, FL., May 11-17, 1980).
Hepatitis Profiling: A Guide to Test Selection. Enoc J. Hollemweguer.
Laboratory Management 26(4):30-34, 1988.
Anti-Nuclear Antibodies: Comparison Study of Enzymeimmunoassay and
Immunofluorescence. Enoc J. Hollemweguer, Greg Mitchell and Martha Lee.
Abstract of 3rd Annual Meeting of the Association of Medical Laboratory
Immunologists, Washington, D.C., August 2-6, 1990.
HLA-B27 Determination by Immunofluorescence as Compared with Lymphocyte
Microcytotoxicity. Enoc J. Hollemweguer, Teri Jackson, Diane Hester and
Janice Arnett. Abstract of 3rd Annual Meeting of the Association of Medical
Laboratory Immunologists, Washington, D.C., August 2-6, 1990.
Autoantibodies to Extractable Nuclear Antigens, a Comparison Study:
Enzymeimmunoassay and Double Immunodiffusion. Enoc J. Hollemweguer, Teri
Jackson, Rhonda Harris and Janice Arnett. Abstract of 3rd Annual Meeting of
the Association of Medical Laboratory Immunologists, Washington, D.C.,
August 2-6, 1990.
Study of T-cytotoxic cells in Normal and HIV infected individuals using the
S6F1 Marker. Enoc J. Hollemweguer, Karen Johnson and Loretta DeWald.
Abstract of 3rd Annual Meeting of the Association of Medical Laboratory
Immunologists, Washington, D.C., August 2-6, 1990.
Yu. J.F., X.F Yang, E. Nguyen, G.X. Lin, E. Hollemweguer, and Z. Chen.
VIIth HLDA workshop cytokine receptor section: flow cytometric analysis of
65 antibodies on 11 different target cells. In: Mason D, Andre P,
Bensussan A, et al, eds. Leucocyte Typing VII: White Cell Differentiation
Antigens. New York, NY: Oxford University Press 2002: 275 - 278.
Yu, J.F., X.F. Yang, L.R. Shi, B.C. Sang, E. Nguyen, E. Hollemweguer, T.
Ward and Z. Chen. Clone D2-1173 is a novel monoclonal antibody against
human CD30L (CD153). In: Mason D, Andre P, Bensussan A, et al, eds.
Leucocyte Typing VII: White Cell Differentiation Antigens. New York, NY:
Oxford University Press 2002: 712-714.
Yu, J.F., X.F. Yang, L.R. Shi, B.C. Sang, E. Nguyen, E. Hollemweguer, T
Ward and Z. Chen. Generation and characterization of two novel monoclonal
antibodies against human 4-1BB ligand. In: Mason D, Andre P, Bensussan A,
et al, eds. Leucocyte Typing VII: White Cell Differentiation Antigens. New
York, NY: Oxford University Press 2002: 714 - 716.
Wu, X.W., E. Hollemweguer, and Z. Chen. SN2: a monoclonal antibody to CD165
induces platelet activation. In: Mason D, Andre P, Bensussan A, et al,
eds. Leucocyte Typing VII: White Cell Differentiation Antigens. New York,
NY: Oxford University Press 2002: 556 - 558.
Wu, X.W., E. Nguyen, E. Hollemweguer, and Z. Chen. Flow cytometric analysis
and functional studies of four monoclonal antibodies from the platelet
blind panel. In: Mason D, Andre P, Bensussan A, et al, eds. Leucocyte
Typing VII: White Cell Differentiation Antigens. New York, NY: Oxford
University Press 2002: 546-548.
Lin, G.X., X.F. Yang, E. Hollemweguer, J.F. Yu, L. Li, X.W. Wu, and Z.
Chen. Cross reactivity of CD antibodies on eight animal species. In:
Mason D, Andre P, Bensussan A, et al, eds. Leucocyte Typing VII: White Cell
Differentiation Antigens. New York, NY: Oxford University Press 2002: 519 -
524.
Effect of monoclonal antibodies to platelet associated antigens on platelet
function. Xiao-wei Wu, Enoc J. Hollemweguer, 13th annual meeting of the
Association of Medical Laboratory Immunology, St. Petersburg, FL, July,
2000.
Monoclonal antibodies raised to human cells-specificity for pig leukocytes.
2001. Haverson, K., Bailey, M., Stokes, C.R., Simon, A., Le fluffy, L.,
Banfield, G., Chen, Z., Hollemweguer, E., Ledbetter, J.A., Vet. Immunol.
Immunopathol. 80: 175-186
Summary of workshop findings for porcine T-lymphocyte-specific monoclonal
antibodies. 2001. Saalmuller, A., Kuebart, G., Hollemweguer, E., Chen, Z.,
Nielsen, J., Zuckerman, F., Haverson, K. Vet. Immunol. Immunopathol. 80: 35-
52.
References
David Ernst, Ph.D.
Associate Director Research & Development
BD Biosciences
10975 Torreyana Rd.
San Diego, CA 92121
accf54@r.postjobfree.com
David Singer, Ph.D.
Biology Dept.
San Diego City College
1313 Park Boulevard
San Diego, CA 92101
accf54@r.postjobfree.com
Sandra Slivka, Ph.D.
Biology Dept.
Miramar College
10440 Black Mountain Rd.
San Diego, CA 92126
accf54@r.postjobfree.com
Daniel Trubovitz, Ph.D.
Biology Dept.
Miramar College
10440 Black Mountain Rd.
San Diego, CA 92126
accf54@r.postjobfree.com
Mr. Robert Fremland
Chemistry Dept.
San Diego Mesa College
7250 Mesa College Dr.
San Diego, CA 92111
accf54@r.postjobfree.com
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