Curriculum Vitae

Giang H. Pham, Ph.D

*** ****** *****, ******, *** York-12054

Cell: 518-***-****; Work phone: 518-***-****

Email: accaxw@r.postjobfree.com; accaxw@r.postjobfree.com

Key capabilities

Extensive experience with immunoassays (ELISAs, CBA assays, ECL assays, Luminex

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assays, T cell biology and proliferation assays, Cytotoxic T lymphocyte killing assays,

dendritic cell biology, primary cell separation and isolation)

Expertise in flow cytometry

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Highly skilled in molecular biology techniques (cDNA library preparation, PCR, qPCR,

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DNA manipulation and cloning, plasmid vector construction, sequence analysis)

Excellent in microbiological techniques (bacterial transformation, selection, culture

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pathogen agents in BSL-2 and BSL-3)

Highly skilled in recombinant protein expression, purification and characterization (SDS-

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PAGE, Western blot)

Expertise in mammalian cells culture (cell lines, primary cell isolation/differentiation, in

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vitro transient and stable cell transfections)

Excellent experience with mouse infection models in BSL-2 and BSL-3 facilities

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Highly proficient at working and managing multidisciplinary projects

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Strong and critical data analysis skills; Excellent data management

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Excellent communication skills (written and presentation)

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Excellent computer literacy and knowledge of software applications (Microsoft Office

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documents, GraphPad Prism, FlowJo, Cellquest Pro, EndNote)

Excellent self-starting researcher and works effectively within a team

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Comfortable in a challenging, fast paced environment

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Experience in postgraduate supervision, laboratory management and organization

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Education

2007 Ph.D in Immunology, International Centre for Genetic Engineering and

Biotechnology (ICGEB), New Delhi, India.

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2003 Master of Science in Microbiology, School of Life Sciences, Jawaharlal Nehru

University (JNU), New Delhi, India.

1999 Engineer of Informatics, Hanoi University of Science and Technology (HUST),

Hanoi, Vietnam.

1994 Bachelor of Sciences Molecular Biology and Physiology, Biology Faculty,

Hanoi University of Science (HUS), Hanoi, Vietnam.

Research Experience

April 2010 - present Center for Immunology and Microbial Diseases, Albany

Medical College, Albany, NY 12208

Position: Postdoctoral Researcher

Responsibilities:

Conducting independent research on targeting Fc gamma receptors on Dendritic Cells

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to improve the immune responses against Francisella tularensis infection using mouse

model

Driving and utilization of ex vivo antigen loaded BMDCs as a new vaccine candidate for

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tularemia in mouse model of F.tularensis LVS infection

Design and implementation of in vitro and in vivo experiments evaluating the

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immunogenicity of inactivated F.tularenis LVS grown in different bacterial mediums

Culture of F. tularensis LVS in BSL-2, highly virulent pathogen F. tularensis SchuS4 in

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BSL-3; Preparation of inactivated F. tularensis for immunization

Implementation immunoassays (cell proliferations, cell maturation and activation,

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antigen binding and internalization, cytokine and antibody ELISAs, CBA, Luminex

assay, ECL assay, Flow cytometry acquisition and analysis)

Establish and optimization of PCR, quantitative RT-PCR protocols in genotyping of

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transgenic mouse

Stable cell transfection and selection of hybridoma cells to produce the fusion proteins

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Troubleshoots assay problems

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First author in two research articles (under preparation) and author in three peer-

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reviewed journal articles (published)

Contribute to organization and management of reagent stocks, standard

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operating protocols in the laboratory

Training of technicians, postdoctoral fellows and postgraduate students on

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specialized equipment: Flow machines (FACSscan, FACSCalibur, LSRII), FACSArray,

PCR-Thermal Cyclers, Luminex

Contribute to scientific activities within the department

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February 2009 - March 2010 Animal Science Department

Oklahoma State University, Stillwater, OK 74075

Position: Postdoctoral Researcher

Responsibilities:

Conducted independent studies on potential of antimicrobial peptides as antibiotic

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alternatives in infectious disease control and prevention in mouse model

Training of students and technicians in flow cytometry, tissue culture, molecular biology,

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immunoassays, and work with mouse model

Supervision of rotation postgraduate students

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Co-first authors in a research article (Accepted for publication, Molecular Immunology)

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December 2007 - December 2008 Division of Immunology

Institute for Infectious Disease and Molecular

Medicine

University of Cape Town, South Africa

Position: Postdoctoral Researcher

Responsibilities:

Investigated independent the role of alternatively activated macrophages and the impact

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of IL-4 receptor alpha in lung fibrosis disease induced by bleomycin

Supervision of undergraduate students

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Grant writing to obtain internal source of funding

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Peer-Reviewed Publications

A. Research Articles

1. G. Pham, C. Bitsaktsis, and E. J. Gosselin. Fc receptor-targeting of immunogen as a

strategy for enhanced antigen loading, vaccination, and protection using intranasally-

administered antigen-pulsed dendritic cells (under preparation).

2. Giang H. Pham, Bibiana V. Iglesias, Edmund J. Gosselin. Fc Receptor-Targeting of

Immunogen as a Strategy for Enhanced Antigen Loading, Vaccination, and Protection Using

Intranasally-Administered Antigen-Pulsed Dendritic Cells (submitted to Vaccine).

3. Yugendar R. Bommineni, Giang H. Pham*, Lakshmi T. Sunkara, Mallika Achanta,

Guolong Zhang. Immune Regulatory Activities of Fowlicidin-1, a Cathelicidin Host Defense

Peptide (Accepted for publication, Molecular Immunology)[*co first-authors].

4. Iglesias BV, Bitsaktis C, Pham G, Drake JR, Hazlett KR, Porter K, Gosselin EJ.

(2012). Multiple mechanisms mediate enhanced immunity generated by mAb-inactiavated F.

tularensis immunogen. Immunol Cell Biol. doi: 10.1038/icb.2012.66.

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5. Bitsaktsis C, Iglesias BV, Li Y, Colino J, Snapper CM, Hollingshead SK, Pham G,

Gosselin DR, Gosselin EJ. (2012). Mucosal immunization with an unadjuvanted vaccine that

targets Streptococcus pneumoniae PspA to human Fcγ receptor type I protects against

pneumococcal infection through complement- and lactoferrin-mediated bactericidal activity.

Infect Immun. 80(3):1166-80.

6. Periasamy S, Singh A, Sahay B, Rahman T, Feustel PJ, Pham GH, Gosselin EJ,

Sellati TJ. (2011). Development of tolerogenic dendritic cells and regulatory T cells favors

exponential bacterial growth and survival during early respiratory tularemia. J Leukoc Biol.

90(3):493-507.

7. Niladri Ganguly, Pham H. Giang, Chitra Gupta, Sandip K. Basu, Imran Siddiqui,

Dinakar M. Salunke and Pawan Sharma (2008). Mycobacterium tuberculosis secretory proteins

CFP-10, ESAT-6 and the CFP10:ESAT6 complex inhibits Lipopolysaccharide induced NF- κB

transactivation by downregulation of reactive oxidative species (ROS) production. Immunol Cell

Biol. 86(1):98-106.

8. Niladri Ganguly, Pham H. Giang, Sandip K Basu, Fayaz A Mir, Imran Siddiqui,

Pawan Sharma (2007). Mycobacterium tuberculosis 6-kDa Early Secreted Antigenic Target

(ESAT-6) protein downregulates lipopolysaccharide-induced c-myc expression by modulating

the Extracellular Signal Regulated Kinases . BMC Immunol., 8:24.

9. Malla R, Prasad R, Kumari R, Giang PH, Pokharel U, Oelueller R and Varma A

(2005). Phosphorus solubilizing symbiotic fungus: Piriformospra indica. Endocytobiosis. Cell

Res, Japan, 15: 579-600.

10. Peskan-Berghoefer T, Shahollari B, Giang PH, Hehl S, Markent C, Blank V, Kost G,

Varma A, Oelmueller R (2004). Association of Piriformospora indica with Arabidopsis thaliana

roots represent a novel system to study beneficial plant-microbe interactions and involve in

early plant protein modifications in the endocytoplasmic reticulum and in the plasma

membrane. Plant Physiology, Holland, 122: 465-471.

11. R. Oelmüller, B. Shahollari, T. Peškan-Berghöfer, A. Trebicka, P.H. Giang, I.

Sherameti, M. Oudhoff, Y. Venus, L. Altschmied, A. Varma (2004). Molecular analyses of the

interaction between Arabidopsis roots and the growth-promoting fungus Piriformospora indica.

Endocytobiosis Cell Res. Japan, 15: 504-517.

12. Kumari R, Pham GH, Sachdev M, Garg AP and Varma A (2004). Symbiotic Fungi

for Eco-friendly Environment: A Perspective. Natural Product Radiance CSIR, India, 3(6): 396-

400.

B. Book chapters

1. Das A, Prasad R, Srivastava A, Pham GH, Bhatnagar K and Varma A (2007) Fungal

Siderophores: Structure, Functions and Regulations. In: Microbial Siderophores (eds Varma A

and Chincholkar S), Springer-Verlag, Germany. 12: 1-42.

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2. Pham GH, Srivastava A, Saxena A K, Pareek A and Varma A (2005) Protocol to

Understand the Interaction Between Rhizobacteria and Symbiotic Fungus: Piriformospora

indica. In: Basic Research and Applications: Mycorrhizae (eds Podila G and Varma A).

Microbiology Series, IK International- India, New York. 1: 425-450.

3. Kumari R, Pham GH, Prasad R, Sachdev M, Srivastava A, Yadav V, Verma P K,

Sharma S, Malla R, Singh A., Maurya A K, Prakash S, Pareek A., Rexer K-H, Kost G, Garg A

P, Oelmueller R, Sharma M C and Varma A (2005) Piriformospora indica: Fungus of the

Millenium. In: Basic Research and Applications: Mycorrhizae (eds Podila G and Varma A).

Microbiology Series, IK International- India, New York. 1: 259-295.

4. Prasad R, Pham GH, Kumari R, Singh A, Yadav V, Sachdev M, Garg AP, Peskan T,

Hehl S, Sherameti I, Oelmüller R and Varma A (2005) Sebacinaceae: Culturable Mycorrhiza–

like Endosymbiotic Fungi and their Interaction with Non-transformed and Transformed Roots.

In: In vitro Culture of Mycorrhizas (eds. Declerck S, Fortin AJ and Strullu DG). Soil Biology

Series, Springer-Verlag, Germany. 4: 291-312.

5. Pham GH, Kumari R, Singh An, Sachdev M, Prasad R, Kaldorf M, Buscot F,

Oelmuller R, Tatjana P, Weiss M, Hampp R and Varma A (2004) Axenic Cultures of

Piriformospora indica. In: Plant Surface Microbiology (eds Varma A, Abbott L, Werner D and

Hampp R), Springer-Verlag, Germany pp. 593-613.

Conference Proceedings

1. AAI annual Meeting: Immunology 2013, May 3-7, 2013, Honolulu, Hawaii.

Oral poster presentation: “Fc receptor-targeting of immunogen as a strategy for

enhanced antigen loading, vaccination, and protection using intranasally-administered antigen-

pulsed dendritic cells”.

2. 15th Annual Upstate New York Immunology Conference, October 21 – 24, 2012,

Bolton Landing, New York.

Oral poster presentation: “Use of antigen-pulsed dendritic dells to further study

immune complex mediated protection against tularemia”.

3. 40th Meeting Season, Keystone Symposia on Molecular and Cellular Biology –

Meeting on Dendritic Cells and the Initiation of Adaptive Immunity, February 12 – 17,

2011, Keystone Colorado.

Oral poster presentation: “Targeting inactivated Francisella tularensis to Fc gamma

receptors on dendritic cells (DCs) enhances DC maturation and secretion of pro-inflammatory

cytokines”.

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