KOMALI GNANA PRAVEENA B

E-mail:acc8e5@r.postjobfree.com Mobile: 900-***-****

Objective:

To seek a challenging position, with potential for usage and advancement of my skills and abilities

being resourceful, innovative and flexible for the organization.

Educational Excellence:

Degree Year Of Board/University % Class

Passing

B.Tech 2006 Jawaharlal Nehru Technological 72 First Class with

Biotechnology University, Hyderabad Distinction

Intermediate 2000 Board of Intermediate 71 First Class

SSC 1998 Board of Secondary Education 82 First Class with

Distinction

Professional Experience: 5yrs

Research Associate (2010- 2011)

@ St. Johns Research Institute, Bangalore.

• Isothermal amplifications (LAMP and Helicase LAMP) with clinical samples (Tuberculosis).

• Literature search for isothermal amplification techniques like LAMP(Loop Mediated

Amplification) and Helicase LAMP etc.,

• Comparative study on 22 types of Isothermal amplifications and prepare a detailed review of

them in specific to Mycobacterium using NCBI Pubmed and Google advanced scholar search.

• Isolation of Mycobacterial DNA from lysed culture.

• Perform Isothermal amplification of the isolated DNA using LAMP in Real time PCR machine

and End point PCR machine.

• Analyse the result on AGE (Agarose Gel Electrophoresis) and Real time PCR machine.

• Integrate use of Helicase to LAMP

• Standardize Helicase LAMP with respect to amount of Helicase to template DNA and buffer

compatibility.

KOMALI GNANA PRAVEENA B

• Perform Helicase LAMP at human body temperature.

• Reverse Transcription PCR

Research Assistant and Lab In charge (2009- 2010)

@ Institute of Bioinformatics and Applied Biotechnology, Bangalore.

• Manuscript writing for paper publication which was communicated to Oxford journal

(Bioinformatics)

• Plan and schedule the course experimental protocols for students.

• Teach Bio techniques to PG diploma students of Bio techniques at wet lab.

• Evaluate performance of students in biotech lab skills, record maintenance, presentations and

exams.

• Lab management and maintenance.

• Explain about various lab equipment and methods to different category of guests like medical

doctors, students etc.,

Paper under publication:

• Title: Ex-Ex Primer: a tool for designing oligonucleotides spanning spliced nucleic acid

regions

• An experimentally validated novel and unique Bioinformatics tool to design specific primers

and probes for different molecular biology experiments had been designed. This tool has

many sub tools which can be used in designing primers for various experiments like Detect of

gene expression, RTPCR while overcoming genomic DNA contamination, cloning, Micro

Array, Real time PCR etc.,

• Communicated to: Bioinformatics

Junior Scientist (2008- 2009)

@ Connexios Life Sciences Pvt Ltd., Bangalore.

• Design primers and probes for real Time PCR according to specifications of the project.

• Standardize primers or probes considering various factors that influence their activity in the

final PCR mixture.

• Finally standardize real-time PCR with the designed primers or probes.

• Troubleshoot End point and Real Time PCR with respect to primers performed by different

assay groups.

KOMALI GNANA PRAVEENA B

• Involved in different cell based assays, Biomarker expressions.

• Some of the cell lines handled: C2C12, HEK, Hep G2 etc.,

• In charge and maintenance of the confidential drug stock of the company.

• RNA isolation from Cell lines

• Reverse Transcription PCR

• Cell culture techniques like Seeding, Reseeding, Drug Induction, transfection, freezing,

Splitting and revival.

• AGE (Agarose Gel Electrophoresis)

Research Assistant and Lab In charge (2007- 2008)

@ Institute of Bioinformatics and Applied Biotechnology, Bangalore.

• Worked for project “Primer Junction”, a novel and unique Bioinformatics tool to design specific

primers and probes for PCR, Micro Array, Real Time PCR etc.,

• Design of primers, validation by Insilico and practical methods to check different parameters

given by the tool.

• Interacted with the software programmers in order to develop this tool. Teaching students of

Laboratory Course in Bio techniques (LCBT) of IBAB in certain aspects like Primer design,

RNA Isolation, PCR and RTPCR, DNA sequencing etc.,

• General Lab management: Check the chemical stock, Interact with different companies to

negotiate about the products prices and request for placing order after comparing the different

company quotations.

• Set up, cleaned and maintained laboratory equipment

• Electrophoresis Techniques (AGE, SDS PAGE)

• DNA Sequencing with Beckman Coulter CEQ 2000 equipment.

Research Assistant (2006- 2007)

@ Institute of Bioinformatics and Applied Biotechnology, Bangalore.

• Manual design of primers for cloning.

• Design of primers with different automated tools like Primer Express (Commercial), Gene

Runner (Free), Oligo analyzer, Primer3 etc.,

• Study on reliable melting temperature (Tm) calculation module.

• RNA and DNA isolation from Plant and Animal tissues

• Polymerase Chain Reactions

KOMALI GNANA PRAVEENA B

• Reverse Transcription PCR

• AGE (Agarose Gel Electrophoresis)

Project Trainee (2005- 2006 i.e., continued project work of final semester of B.Tech

Biotechnology) @ Institute of Bioinformatics and Applied Biotechnology, Bangalore.

• Expertise with Bioinformatics tools like BLAST, Web cutter, Neb Cutter etc.

• Familiar in using Bioinformatics databases like NCBI, REBASE, and ENSEMBL etc.

• Design of PCR Cycle.

Other wet lab skills:

• Western Blot

• Southern Blot

• SDS PAGE

Related Skills:

• Micro Array Technique: undergone theoretical sessions at IBAB by Ocimum Biosolutuions.

• General Lab Practices: Theoretical sessions on GLP and GMP by Lotus organization.

Skills exposed to:

• HPLC

• 2 D Gel Electrophoresis (BIO Rad)

Computer Skills:

• Fundamentals of 'C'

• Basic computer skills.

Other Skills:

• Effective management, technical and supervisory

KOMALI GNANA PRAVEENA B

• Public speaking and presentation experience

• Work well as a team member or independently.

• Use clear, concise communication skills in conjunction with organizational skills to perform

daily duties.

• Performance Evaluations

Poster presentation:

• A poster which includes the Oligo Junction was presented in Bangalore- Bio2007 (June 7, 8,

9th of 2007).

Workshops and Symposiums:

• Workshop in Recombinant DNA Technology at Institute of Bioinformatics and Applied

Biotechnology, Bangalore for twenty days.

• DNA sequencing by Beckman Coulter Company for a week at IBAB.

• Real time PCR: Undergone theoretical and practical sessions by Eppendorf Real Time PCR

equipment.

• Active participation in Bangalore Bio ’07.

• Paper presented at National Level Symposium on “The Effects of Cholesterol and the

methods to reduce cholesterol”.

Academic project work:

Title: Specialized Primer Designing: Comparison of Automated Vs. Manual Processes.

• Team Size : One

• Duration : Two months

• Institution : Institute of Bioinformatics and Applied Biotechnology, Bangalore

Description: I was involved in an original ongoing research work conceptualized and initiated by

Dr. Kshitish Acharya, Faculty scientist, IBAB. This project provides the information as to what the

molecular biologists need for a primer to be designed and how these requirements can act as

guidelines in developing a novel primer designing tool.

Mini project:

Title: Effects of cholesterol and the methods to reduce cholesterol.

• Team Size : Three

• Duration : One month

KOMALI GNANA PRAVEENA B

• Institution : Madanapalle Institute of Technology and Science (MITS)

Description: Three rabbits were selected for this project, one as Control, second fed with

cholesterol along with normal diet and another with cholesterol, normal diet and treatment. The

cholesterol concentration before treatment and after treatment was calculated in liver, kidney and

blood using biochemical experimental methods. The treatment has given satisfactory results.

Areas of interest:

• Molecular Biology

• Genomics

• Bioinformatics

Extra-curricular activities:

• President for Interact Rotary club at school level during 1997-98.

• President for the Association of Biotechnology at college level during 2003-05.

• Editor, representing the Dept. of Biotechnology for the college English newsletter.

• Championship in games and sports at school level.

• Active participation in quiz, elocutions, essay writings at school and college level.

• Anchored for most of the college functions.

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