Medical Years Experience
Resume:
JON F. TALLY
*** ** **** ***** ***** Home: 816-***-****
Lee’s Summit, MO 64082 Cell: 816-***-****
*********@*****.***
EXECUTIVE SUMMARY:
Jon Tally is a senior scientist with over 25 years experience developing innovative
experimental solutions to explore biochemical properties of proteins. He is proficient
in protein purification in gram quantities, assay development, electron microscopy,
analytical method troubleshooting and documented expertise in protein-ligand
interaction both quantitatively and qualitatively as a purification strategy.
PROFESSIONAL QUALIFICATIONS:
• Skilled protein structure scientist and innovative biochemical analyst.
o Developed new and troubleshooted standard methods for purification of
gram quantities of numerous protein species from a variety of prokaryotic
and eukaryotic organisms.
o Proven experience and aptitude for quantifying protein- ligand
binding kinetics using spectroscopy, microcalorimetry and biolayer
interferometry in multiple protein-ligand systems.
o Trained associates in new techniques of biochemical analysis: high
resolution electron microscopy, protein purification, enzyme kinetics,
UV-Vis and fluorescence spectroscopy, biolayer interferometry and
isothermal titration microcalorimitry.
o Maintained and monitored calibration of laboratory instrumentation and mentored
technicians in proper care and use of analytical instrumentation.
• Recent Scientific achievements
o First to produce and visualize by electron microscopy the formation of
hydrophobic pore forming assemblies of Tetanus neurotoxin inserted into
lipid nanodisks.
o Determined the pH and concentration dependence of bacterial toxin
hydrophilic to hydrophobic transition using bio-layer interferometry.
o Published numerous peer reviewed articles in frontline scientific journals
describing the kinetics of transient substrate species of enzyme reactions.
EDUCATION:
M.S. - Microbiology, University of Missouri Kansas City, Kansas City, MO
B.S. - Biology, University of Missouri Kansas City, Kansas City, MO
AFFILIATIONS:
American Chemical Society (ACS) (2008 – present)
JON F. TALLY
808 SW Lake Pines Drive
Lee’s Summit, MO 64082
*********@*****.***
American Association for the Advancement of Science (AAAS) (2005 – present)
EMPLOYMENT HISTORY:
Visiting senior scientist volunteer, UNIVERSITY OF KANSAS MEDICAL
CENTER, Kansas City, KS (April, 2013 – July, 2013)
• Visiting senior scientist exploring protein-ligand binding using biolayer
interferometry.
Senior Research Associate UNIVERSITY OF KANSAS MEDICAL CENTER,
Biochemistry and Molecular Biology, Dr. Mark Fisher Laboratory, Kansas City, KS
(August, 2011 - March, 2013)
• Developed methods for transformation of hydrophilic monomers to hydrophobic
oligomers of the membrane insertable form of Tetanus toxin for ligand binding
studies using biolayer interferometry and high resolution electron microscopy.
• Directed protein purification using a variety of gel chromatography media using
AKTA (GE) and Bio-Rad controllers. Many proteins were used for biochemical
analysis of proteins implicated in protein miss-folding disease and recovery of
proteins trapped in insoluble inclusion bodies.
Senior Research Associate UNIVERSITY OF KANSAS MEDICAL CENTER,
Biochemistry and Molecular Biology, Dr. Harvey Fisher Laboratory, Kansas City, KS
(August 2006 - August 2011)
• Supervised production of numerous cloned enzyme mutants by gel filtration and
affinity chromatography.
• Designed experiments and interpreted the kinetics of deuterium substrate isotope
and solvent isotope effects to elucidate the reaction mechanism of various enzymes.
Managing Stockholder LAKE PINES ASSOCIATES, Lee’s Summit, MO
(January, 2005 - July, 2006)
• Co-owned and operated a Car-X automotive repair franchise during a break of
NIH funding.
Senior Research Associate VETERANS AFFAIRS MEDICAL CENTER and
UNIVERSITY OF KANSAS MEDICAL CENTER, Kansas City, KS (1992 - 2004)
2
JON F. TALLY
• Designed experimental procedures and interpreted results of investigations related
to enzyme structure, function and mechanism viewed through UV-Vis
spectroscopy, electron microscopy and micro-calorimetry.
• Purified gram quantities of various dehydrogenase species from anaerobic cell
cultures as well as E.Coli mutants.
3
Contact this candidate