Software Project
Resume:
Hollie E. Flick, Ph.D.
Philadelphia, PA 19130
************@*****.***
www.linkedin.com/in/hollieflick/
QUALIFICATIONS
Highly motivated and hard working individual with exceptional interpersonal skills.
Adaptable and resourceful with the ability to learn quickly and efficiently.
Possesses scientific knowledge and experience in various fields including
cellular/molecular biology, biochemistry, oncology and immunology
Experienced in pharmaceutical brand name development and project management
Encouraging leader and mentor while also being a supportive team player and
collaborator.
EDUCATION
Ph.D.: Drexel University College of Medicine, Philadelphia, Pennsylvania 2006-2013
Biochemistry
Title: Targeting the Immune Modulating Enzyme IDO1 Using Anticancer Agents
Degree Received: May 2013
B.A.: Capital University, Columbus, Ohio 2002-2006
Major: Biochemistry
Minor: Biology
Degree Received: May 2006
University of Paris/La Sorbonne, Paris, France 2004
Cultural Experiences Abroad Program
RESEARCH EXPERIENCE
Doctoral Student, LIMR and DUCOM 2011-2013
Studied the immune modulating enzyme IDO1 in enzyme assays and in cell culture.
Uncovered potential therapeutic inhibitors of IDO1.
Identified a novel induction pathway of IDO1.
Mentored graduate and undergraduate students
Advisor: Dr. Alexander J. Muller
Doctoral Student, DUCOM 2007-2011
Studied changes in cytokine profiles in response to IDO1 expression in cell culture.
Used an ecdysone inducible system to express IDO1 in B cells.
Studied effect of IDO1 expression in mixed lymphocyte reactions.
Studied serotonin as an autocrine growth factor in the context of cancer.
Mentored graduate and undergraduate students
Advisor: Dr. Bradford Jameson
Undergraduate Researcher, Capital University, Chemistry Department 2005-2006
Used DNA microarray analysis to study the diauxic shift of yeast.
Optimized protocol for DNA microarray analysis.
Evaluated various microarray analysis software packages.
Advisor: Dr. Jens Hemmingsen
Undergraduate Researcher, Capital University, Chemistry Department 2005
Studied methods for protein purification and site-directed mutagenesis of D. Vulgaris
Flavodoxin.
Advisor: Dr. Tracey Murray
Undergraduate Researcher, Capital University 2004
Developed a functional laboratory protocol for 2-D gel electrophoresis to obtain optimal
results for use in a biochemistry laboratory.
Advisor: Dr. Jens Hemmingsen
RELEVANT EXPERIENCE
Director of Brand Development, Brand Institute 2013-Present
Help to define and oversee branding strategies from early stages of product
development through submission to regulatory authorities
Work closely with C-level executives and global operations teams to ensure multiple
project deliverables are met to the highest standards and within time frames
Lead strategic and creative workshops with clients
Identify and develop new business opportunities
Develop and maintain client relationships
SKILLS
Lab Skills:
Protein: SDS PAGE, protein quantification, protein purification, immunoprecipitation, western
blot analysis, HPLC, FPLC, and enzyme kinetics
Molecular and Cellular Biology: cell culture, qPCR, RT-PCR, site-directed mutagenesis, cloning,
shRNA, siRNA, DNA and RNA purification, transfection, electroporation, proliferation assays
Immunology: ficoll gradients, mixed lymphocyte reactions, FACS, immunoflorescence, and
immunohistochemistry
Computer Skills: MS Office, Adobe Photoshop, Sigma Plot, Amira Software (microCT analysis),
End Note, Image Lab (BioRad Imager), SDS Software (real-time PCR), and blast searches
PUBLICATIONS
Flick, H.E., DuHadaway J.B., Muller, A.J. Immunotherapeutic Targeting of Cancer Signaling
Pathways with Sorafenib to Block IDO. Manuscript in preparation.
Flick, H.E., LaLonde, J.M., Malachowski, W.P., Muller, A.J. Tumor-Selective Cytotoxic Agent -
lapachone is a Potent Inhibitor of Indoleamine 2,3-Dioxygenase. Accepted by the
International Journal of Tryptophan Research.
Smith, C., Chang, M. Y., Parker, K. H., Beury, D. W., Duhadaway, J. B., Flick, H. E., Boulden,
J., Sutanto-Ward, E., Soler, A. P., Laury-Kleintop, L. D., Mandik-Nayak, L., Metz, R.,
Ostrand-Rosenberg, S., Prendergast, G. C., Muller, A. J. (2012). IDO Is a Nodal
Pathogenic Driver of Lung Cancer and Metastasis Development. Cancer Discov., 2(8),
722-735.
PRESENTATIONS
CRI International Cancer Immunotherapy Symposium, NYC, NY 2012
Poster: Immunotherapeutic Targeting of Cancer Signaling Pathways to Block IDO
DUCOM Annual Discovery Day, Philadelphia, PA 2012
Poster: Immunotherapeutic Targeting of Cancer Signaling Pathways to Block IDO
DUCOM Annual Discovery Day, Philadelphia, PA 2011
Poster: IDO Nullizygous Mice Resist Oncogenic KRAS-Induced Pulmonary
Adenocarcinoma
DUCOM Annual Discovery Day, Philadelphia, PA 2010
Poster: IDO Influences the Cytokine/Chemokine Expression Profile of Immune Cells
DUCOM Annual Discovery Day, Philadelphia, PA 2008
Poster: Colorectal Cancer Cells, Colo 320 DM, Require Serotonin Signal for Survival
National Conference on Undergraduate Research, UNC Asheville, NC 2006
Oral presentation: Comparison of the Image Analysis Software for DNA Microarrays
Symposium for Undergraduate Research, Capital University, Columbus, OH 2006
Poster: Site-Directed Mutagenesis of D. Vulgaris Flavodoxin
OTHER CONFERENCES ATTENDED
Immunochemotherapy: Correcting Immune Escape in Cancer, Philadelphia, PA 2011
Era of Hope Meeting for the D.O.D. Breast Cancer Research Program 2008
Baltimore, MD
HONORS AND AWARDS
Capital University Symposium on Undergraduate Scholarship 2006
PLTL Conference Travel Scholarship 2006
Dean’s List 2002-2006
Collegiate Fellow Recognition Award 2002-2006
Capital University Trustees Scholarship 2002-2006
Bey Scholarship (academic achievement in chemistry) 2004
Hendershot Pre-Med Scholarship 2003
References
Available Upon Request
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