IHC, molecular biology, and cell culture techniques

Resume

Soniya A. Parulekar, B.S., M.S.

Address: *** **** ****, *** *-442, Devon, PA 19333

Phone: 484-***-****, E-mail: acc4yw@r.postjobfree.com

Objective: To obtain a position of responsibilities that utilizes my skills and experience while working in an environment where I can enrich my knowledge and provide valuable input by being an asset to the company.

Education

MS in Biological and Pharmaceutical Biotechnology 08/2011-09/2013

Institute for Biotechnology, St. John’s University, Queens, NY

GPA: 3.97/4.0, highest in class

BS in Biotechnology, Department of Biotechnology 06/2008-05/2011

Dhempe College of Arts and Science, Goa University, Goa, INDIA

Graduated with Distinction, highest rank in class

Educational Honors

• Teaching assistantship for highest GPA, Institute for Biotechnology,

St. John’s University 08/2012-09/2013

• BS Biotechnology award of excellence for highest rank in class, Dhempe College of Arts and Science,

Goa University, India 12/2011

•Outstanding student of the year, Dhempe College of Arts and Science, Goa University, India 12/2010

Research Experience

Regeneron Pharmaceuticals, Tarrytown, NY 01/2013-08/2013

Graduate Intern (Co-operative study)

As part of technology development, successfully determined a suitable fluorescent marker to test for beta-

galactosidase expression in mice tissue required for further optical projection tomography research. This involved

working with mice in order to derive tissues, organs and embryos, followed by their further processing by fixation,

embedding, cryo-sectioning, staining, setting up positive and negative controls, immunohistochemistry and imaging

with bright field and fluorescent microscopy to detect targets for gene expression.

Supervisor: Dr. Johnathon Walls, Department of Pre-Therapeutic Target Discovery

St. Johns University, Queens, NY 07/2012-01/2013

Graduate Student Researcher

Worked on an on going research project for creating new mutants, by removal of transposons and producing new

alleles by transposon mutagenesis in Drosophila melanogaster.

Supervisor: Dr. Chris Bazinet, Department of Genetics of Biological Assemblies

Tulip Diagnostics, Verna, Goa, INDIA 06/2010-01/2011

Project Assistant

Assisted in an immunology project wherein mono-specific anti-sera against salmonella species, was prepared for

its flagella and cell wall antigens AH, BH, AO and BO.

Campal Clinic, Panaji, Goa, INDIA 05/2010

Summer Trainee – Assisted in performance of pathological tests for HIV, pregnancy, blood grouping, typhoid

(Widal test), blood count, hemoglobin count and malaria

Resume Parulekar, B.S., M.S.

Teaching Experience

• Adjunct Professor, Biology Department, St. Johns University 08/2011-12/2012

Taught Fundamentals of Biology I and II laboratory courses to undergraduate students. Course included concepts

in molecular biology techniques of transformation, PCR, gel electrophoresis.

• Tutored High School Students in Chemistry and Biology 06/2009-03/2010

Created lessons and quizzes for Mushtifund high school, India, students to improve their comprehension of

chemistry and biology

Seminars/ Conferences Attended

• Life sciences summit, NY, Dec 2012

• Introduced speakers during the 10th anniversary symposium at the institute of Biotechnology, St. John’s

University, May 2012

• New York Biotechnology Association 21st annual Meeting, May 2012

Skills

• Cell Culture Techniques and Toxicology

Establishment of primary cultures Karyotyping Multiplex cytotoxicity assay

MTT and NRU in vitro viability assay Cell count Passaging of confluent monolayers of cultured cells

Flow cytometry Cell proliferation assay Apo logix caspase detection assay

• Molecular Biology and Genetic Engineering

PCR UV survival curve Agarose gel electrophoresis and PAGE

Isolation of plasmid, DNA and RNA

Transformation of e-coli and yeast cells with recombinant DNA technology

• Microbiology

Isolation of bacteria by streak method Enumeration techniques

Staining and identification: gram, monochrome, negative and spore

• Immunology

Differential count of WBC Preparation of serum Immuno-electrophoresis Immunodiffusion methods

Sandwich ELISA

• Other Skills

OCT embedding Cryo-sectioning Immunohistochemistry Lac Z staining

Mice embryo and organ harvesting UV-Vis spectrometry HPLC

Fluorescent microscopy and bright field imaging Perfusion technique Centrifugation techniques

Extra Curricular Activities

• Volunteer teacher, meditation classes, Sri Chinmoy international meditation center (2006-07)

• Given vocal performances at the United Nations headquarters, NY as part of the Sri Chinmoy international music

group (2005-2007)

• Performed in the Manasi arts cultural dance group at Chicago, New York, Princeton NJ, Tampa and Orlando

(2011-2012)

Additional Skills

• Computer: Microsoft Word, Power Point, Excel, Photoshop and Image J

• Languages: English, Hindi, Konkani and Marathi

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