Medical Project

Jie-Gen Chen

I. Personal Data

Place of Birth: P.R. China

Visa Status: Permanent Residency

Home Address: *** ******* **, ****** ****, NC 27514

Home / Cell Phone: 919-***-**** / 919-***-****

Email: ******.****@*****.*** or ******.****@****.***

II. Education

2003. 09 – 2006. 07: Ph.D. in Biochemistry and Molecular Biology,

advised by Prof. Qi-Qun Tang, Shanghai Medical

College, Fudan University, Shanghai, China

(This is the equivalent of the U.S. degree of Doctor

of philosophy in Biochemistry and Molecular Biology

earned at a regionally accredited institution of

higher education in the United States)

2000. 09 – 2003. 07: Master of Medicine in Clinical Laboratory Science,

advised by Prof. Jian-Xin Lu, Wenzhou Medical

College, Zhejiang, China

(This is the equivalent of the U.S. degree of Master

of Science in Medical Laboratory Technology earned

at a regionally accredited institution of higher

education in the United States)

III. Research

2007.02 – 2014.02: Postdoctoral Research Associate, Department of

Surgery, Duke University.

Working on a NIH project (K23DK075907): “Impact

of GBS on CVD in Type 2 Diabetes Mellitus”; a

Research Project Awarded from the Society of

American Gastrointestinal and Endoscopic Surgeons

(SAGES): “Effect of Obesity on Adipogenic

Differentiation of Adipose Tissue-Derived Human

Mesenchymal Stem Cells” and a project (R21CA-

128692) “In Vivo Selection of Tumor- Specific RNA

Binding Motifs”

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2003.09 – 2006.07: PhD candidate in Department of Biochemistry, Fudan

University School of Medicine.

Working on a project from National Natural

Scientific Foundation of China (30270319):

“Regulation of PAI-1 Gene Expression during

3T3-L1 Adipocyte Differentiation”

2000.09 – 2003.07: Graduate student in School of Laboratory Medicine

and Life Science, Wenzhou Medical College.

Working on a project from National Hi-Tech

Research and Development Program of China (No.

2001AA215051): “The Expression Product of

β-Lactamase/Aminoglycoside Inactive Enzyme

Fusion Gene and Its Application”

IV. Funding

1. Research Project Awarded from SAGES “Effect of Obesity on

Adipogenic Differentiation of Adipose Tissue-Derived Human

Mesenchymal Stem Cells” (2008), Co-investigator.

V. Publications

Articles:

1. Khoo CM, Muehlbauer MJ, Stevens RD, Pamuklar Z, Chen J,

Newgard CB, Torquati A. Postprandial Metabolite Profiles Reveal

Differential Nutrient Handling After Bariatric Surgery Compared With

Matched Caloric Restriction. Ann Surg. 2013 Jun 19. [Epub ahead of

print] PMID:23787216.

2. Khoo CM, Chen J, Pamuklar Z, Torquati A. Effects of Roux-en-Y

Gastric Bypass or Diabetes Support and Education on Insulin

Sensitivity and Insulin Secretion in Morbidly Obese Patients With

Type 2 Diabetes. Ann Surg. 2013 May 31. [Epub ahead of print]

PMID:23732262.

3. Chen JG, Spagnoli A, Torquati A. Adipogenic differentiation of

adipose tissue-derived human mesenchymal stem cells: effect of

gastric bypass surgery. Surg Endosc. 2012, 26(12): 3449-56.

4. Chen J, Pamuklar Z, Spagnoli A, Torquati A. Serum leptin levels are

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inversely correlated with omental gene expression of adiponectin and

markedly decreased after gastric bypass surgery. Surg Endosc. 2012,

26 (5): 1476-80.

5. Chen J, Spagnoli A, Torquati A. Omental Gene Expression of

Adiponectin Correlates with Degree of Insulin Sensitivity Before and

After Gastric Bypass Surgery. Obes Surg. 2012, 22 (3):472-7.

6. Pamuklar ZN, Chen J, Muehlbauer M, Spagnoli A, Torquati A.

Necdin-E2F4 interaction provides insulin-sensitizing effect after

weight loss induced by gastric bypass surgery. Surg Obes Relat Dis.

2013, 9 (1): 94-9.

7. Li X, Huang HY, Chen JG, Jiang L, Liu HL, Liu DG, Song TJ, He Q,

Ma CG, Ma D, Song HY, Tang QQ. Lactacystin Inhibits 3T3-L1

Adipocyte Differentiation through Induction of CHOP-10 expression.

Biochem Biophys Res Commun. 2006, 350 (1):1-6.

8. Chen JG, Li X, Huang HY, Liu HL, Liu DG, Song TJ, Ma CG, Ma D,

Song HY, Tang QQ. Identification of a Peroxisome Proliferator

Responsive Element (PPRE)-like Cis-element in the Mouse

Plasminogen Activator Inhibitor-1 Gene Promoter. Biochem Biophys

Res Commun. 2006, 347 (3):821-6.

9. Bin-Bin Zha, Li Zhang, Dong-Hai Jiao, Lin Jiang, Jie-Gen Chen and

Yi-Ming Li. Effect of Refined Rhubarb Tablet on Metabolic

Parameters and Energy Metabolism in Skeletal Muscle of High Fat

Diet Induced Rats. Shanghai Journal of Traditional Chinese Medicine.

2006, 40 (10): 57-9.

10. Dou Jinmin, Guo Lili, Liu Honglei, Chen Jiegen, Huang Haiyan,

Song Houyan and Tang Qiqun. Cloning, Expression, Purification and

Characterization of Recombinant Human Ciliary Neurotrophic Factor

Genes. Journal of Fudan University (Medical Science). 2005, 32 (4):

419-26.

11. Chen JG, Lu JX, Lou YL, Peng Y, Chen XS, Lu YS. Conserved

Region Analysis of aac(3)-II Gene from E.coli Aminoglycoside

Resistance Strain. Hereditas (Beijing). 2004, 26 (2): 202-4.

In Progress:

12. Jiegen Chen, Jean Paul Kovalik, Anna Spagnoli, Zehra Pamuklar

and Alfonso Torquati. Regulation of RBP4 effect on insulin response

by STRA6 in human adipocyte. (Poster Presented on 2010 and 2011

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annual meeting of the Endocrine Society)

13. Jiegen Chen, Anna Spagnoli, and Alfonso Torquati. Expression of

Visfatin/PBEF/Nampt in Visceral Adipose Tissue Normalizes After

Gastric Bypass Surgery. (Poster Presented on 2008 annual meeting of

the Endocrine Society)

14. Jiegen Chen, Anna Spagnoli and Alfonso Torquati. Zfp423 in

Human Adipose Stem Cell. (Poster Presented on 2011 annual meeting

of the Obeisty Society)

Abstracts:

1. Jiegen Chen, Anna Spagnoli and Alfonso Torquati. Effect of Obesity

on Adipogenic Differentiation of Adipose Tissue-Derived Human

Mesenchymal Stem Cells. Surg Endosc. 2012; 26: S186.

2. Chen J.G, Spagnoli A, Pamukla Z and Torquati A. Regulation of

RBP4 effect on insulin response by STRA6 in human adipocyte.

Endocr Rev 2011, 32: P2-522.

3. Chen Jiegen, Pamuklar Zehra, Spagnoli Anna and Torquati Alfonso.

Zfp423 in Human Adipose Stem Cell. Obesity. 2011; 19 (Suppl 1):

S73.

4. Chen J.G, Spagnoli A, Pamuklar Z and Torquati A. Adipose

expression of Stra6 gene: correlation with insulin sensitivity. Endocr

Rev. 2010; 31 (3) Suppl 1.

5. Chen Jiegen, Torquati Alfonso, Pamuklar Zehra and Spagnoli Anna.

Retinol-binding protein 4: impact of circulating and tissue expression

levels on adiponectin. Obesity. 2010; 18 (Suppl 2): S141.

6. Sarah Evans, Jiegen Chen, Zehra Pamuklar and Alfonso Torquati.

Serum Leptin Level are Inversely Correlated with Omental Gene

Expression of Adiponectin and are Markedly Decrease After

Laparoscopic Gastric Bypass Surgery. Surg Endosc. 2009; 23: S151.

7. Spagnoli A, Chen J, Pamuklar Z.N, Granero-Molto F and Torquati A.

Uncarboxylated Osteocalcin is an Endocrine Link between the

Skeleton and the Glucose Metabolism: Clinical Evidences from Obese

and Post Gastric By-pass Patients. J Bone Miner Res. 2008, 23: S125.

8. Jiegen Chen, Anna Spagnoli, Sherry Kernodle, William Richards and

Alfonso Torquati. Surg Endosc. 2008, 22: S149.

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VI. International Meeting Presentation

Oral Presentation:

1. Jiegen Chen, Anna Spagnoli and Alfonso Torquati. Effect of Obesity

on Adipogenic Differentiation of Adipose Tissue-Derived Human

Mesenchymal Stem Cells. Society of American Gastrointestinal and

Endoscopic Surgeons (SAGES) Scientific Session & Postgraduate

Course, San Diego, CA, March 7-10, 2012.

2. Zehra Pamuklar, Jiegen Chen, Anna Spagnoli, Alfonso Torquati.

Insulin-sensitizing effects of gastric bypass surgery (GBS) are

mediated through the necdin-E2F4 interaction. 28th ASMBS Annual

Meeting in Orlando, FL, June 12-17, 2011.

3. Sarah Evans, Jiegen Chen, Zehra Pamuklar, Alfonso Torquati. Serum

leptin levels are inversely correlated with omental gene expression of

adiponectin and are markedly decrease after laparoscopic gastric

bypass surgery. Society of American Gastrointestinal and Endoscopic

Surgeons (SAGES) Scientific Session & Postgraduate Course,

Phoenix, AZ, April 22–25, 2009.

4. Jiegen Chen, Anna Spagnoli, Sherry Kernodle, William Richards,

Alfonso Torquati. C-reactive protein concentration before and after

gastric bypass surgery inversely correlate with omental gene

expression of adiponectin. Society of American Gastrointestinal and

Endoscopic Surgeons (SAGES) Scientific Session & Postgraduate

Course, Philadelphia, PA, April 9–12, 2008.

Poster Presentation:

5. Khoo Chin Meng, Pamuklar Zehra, Chen Jiegen, Park Chan W,

Muehlbauer J Michael, Newgard B Christopher and Torquati Alfonso.

Early Changes in Metabolic and Hormonal Dynamics Following

Roux-en-Y bypass. Later break poster presentation. 71st ADA

Sessions in San Diego, CA, June 24-28, 2011.

6. Chen J.G, Spagnoli A, Pamuklar Z and Torquati A. Zfp423 in Human

Adipose Stem Cell. 29th Obesity Society Annual Scientific Meeting in

Orlando, FL, Oct 1-5, 2011.

7. Chen Jie-Gen, Spagnoli Anna, Pamuklar Zehra and Torquati Alfonso.

Regulation of RBP4 effect on insulin response by STRA6 in human

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adipocyte. 93th Annual meeting of the Endocrine Society in Boston,

MA, June 4-7, 2011.

8. Chen J.G, Spagnoli A, Pamuklar Z and Torquati A. Retinol-binding

protein 4: impact of circulating and tissue expression levels on

adiponectin. 28th Obesity Society Annual Scientific Meeting in San

Diego, CA, Oct 8-12, 2010.

9. Chen J.G, Spagnoli A, Pamuklar Z and Torquati A. Adipose

expression of Stra6 gene: correlation with insulin sensitivity. 92th

Annual meeting of the Endocrine Society in San Diego, CA, June

19-22, 2010.

10. Spagnoli A, Chen J, Pamuklar Z.N, Granero-Molto F, Torquati A.

Uncarboxylated Osteocalcin is an Endocrine Link between the

Skeleton and the Glucose Metabolism: Clinical Evidences from Obese

and Post Gastric By-pass Patients. 30th ASBMR Annual meeting in

Montréal, Québec, Canada, Sept 12-16, 2008.

11. Chen Jie-Gen, Spagnoli Anna, Granero-Molto Froilan, O’Rear Linda

and Torquati Alfonso. Visfatin Expression in Visceral Adipose Tissue

Normalizes after Gastric Bypass Surgery: Correlation with C-reactive

Protein but not with Insulin Sensitivity. 90th Annual meeting of the

Endocrine Society, San Francisco, CA, June 15-18, 2008.

VII. Reference

1. Alfonso Torquati, MD (Postdoc supervisor)

Chief, Metabolic and Weight Loss Surgery

Director, Duke Center for Metabolic and Weight Loss Surgery

Associate Professor, Department of Surgery, Duke University Medical

Center

407 Crutchfield Street, Durham, NC 27704

Phone: 919-***-****; Fax: 919-***-****

E-mail Address:

*******.********@****.***

2. Qi-Qun Tang, MD, PhD (PhD supervisor)

Adjunct Professor, Department of Biochemistry, School of Medicine,

Johns Hopkins University

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Professor and Director, Department of Biochemistry and Molecular

Biology; Key Laboratory of Molecular Medicine, Ministry of

Education

Dean, Fudan University Basic Medical School

P.O.Box 238, 138 YiXueYuan Road, Shanghai 200032, P.R China

Phone: 086-21-54237198; Fax: 086-21-64033738

E-mail Address:

******@****.***.**

3. Martha Delahunty, PhD (Research Colleague)

Research Scientist, Department of Medicine, Duke University

Medical Center

Box 2615, Meidcal Center, Research Drive, MSRB1 Room 337,

Durham, NC 27710

Phone: 919-***-****;

E-mail Address:

******@****.***

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