Nrusingh Prasad Mohapatra, Ph.D

**, ********* **, *************, **-24073

Ph. # 614-***-****, acc4k5@r.postjobfree.com

I have a Ph.D in Medical Microbiology with more than 11 years of research

and laboratory management experience in development of therapeutics,

vaccines, and diagnostics against human diseases. I have extensive

knowledge and hands-on experience in immunology, cell biology, molecular

biology, with bioinformatics (in-silico) analysis of proteins and RNA for

drug discovery against human diseases. I have used different cell culture

and animal models for discovery of new technologies to cure wound healings

in diabetic patients. I have used different cell receptor agonists and

antagonists to study the host-pathogen interactions during microbial

infection process in BSL-2 and BSL-3 laboratories. I have extensive

experience in isolation and handling of human and murine monocytes,

macrophages, neutrophils and dendritic cells for in vivo and in vitro

studies. I have used mouse model for discovery of new vaccines and

therapeutics against several human pathogens in ABSL-2 and ABSl-3

facilities. I have more than 10 years of experience in supervising and

mentoring the post-doctoral fellows, graduate, M.S., undergraduate students

and staffs in different laboratory environments. I have published 19 papers

and presented more than 35 research findings in scientific meetings. I have

used different institutional ordering processes for procurement of

chemicals, and instruments from external and internal vendors. I have

maintained and trained the people for instrument/equipment operations in

BSL-2 and BSL-3 laboratories. I have written several grant proposals

(Academics and SBIR) for Federal funding (NIH/DoD). I have written several

SOPs and manuals in compliance with laboratory, department and institutions

for maintaining the Federal guidelines for laboratory operation, disposal

of biological, chemical and radiological wastes according to the OSHA, CDC,

and FDA regulations. I have prepared documents for BSL-2 and BSL-3

laboratories, IBC approval, and CDC inspection.

Skills

Tissue culture of human and mouse lymphocytes, dendritic cells,

macrophages, and neutrophils; -flow cytometry; -microscopy (Confocal,

Electron, Scanning); -ELISA (cytokine, antibody titers); -phospho-

kinase/protein arrays; -DNA & RNA isolation; -DNA and RNA sequencing; -SDS-

PAGE; -protein-2D-DIGE; -Western blot; -Southern blot; -Northern blot;

-microbial cloning; -microbial mutagenesis; -qRT-PCR; -next generation

sequencing (RNA & DNA); -CHIP-CHIP; -RNA-RNA; RNA-protein; DNA-protein

interactions; -large scale protein purification; -microbiology techniques;

-aseptic techniques; -microbial culture maintenance; -isolation,

characterization and identification of microbes; -phage display;

-chromatography (affinity, TLC, ion-exchange); -LPS isolation and LCMS;

-Radio-labeling and DIGE labeling of DNA, RNA and proteins; animal

experiments in BSL-2 and BSL-3 facilities.

Management / Supervisor Skills

. I have managed and spearheaded the grant funding and group of more than

10 people in industry and academics.

. I have performed internal verification of standards; validated the test

methods and protocols.

. I have identified, invented, developed and implemented effective

solutions to technical issues based on scientific knowledge, evaluation

of the scientific literature and laboratory research.

. I have identified and resolved problems in a timely manner and completed

the tasks on time or notified appropriate person for alternate solutions.

Education

Doctorate of Philosophy (Ph. D) Medical Microbiology Delhi

University, India June 2004

Master of Science (MS) Microbiology, OUAT,

Bhubaneswar, India March 1995

Bachelor of Science (BS) Microbiology, Botany, Chemistry OUAT,

Bhubaneswar, India June 1991

Work Experience

Lynntech Inc. College Station, Texas September 2012-Present

Microbiology Head,

Life Science Department

Scientist II

Accomplishment: Submitted NIH/DoD Small business Industrial Research (SBIR)

grants. In-charge of the Microbiology group, performing independent

research in diverse field of microbial pathogenesis, vaccine development,

drug discovery, biomarker development and therapeutic innovation against

multiple diseases.

The Ohio State University, Columbus, Ohio December 2003-August 2012

Department of Microbial Infection and Immunity

Center for Microbial Interface Biology

Research Scientist December 2006-August 2012

Accomplishment: Published 11 papers, presented 14 posters / oral, Awarded 2

best posters / awards, Associated with writing 2 grants, received funding

from NIH, supervised Ph.D, Master, M.D students, submitted grant reports.

Design and perform in vivo and in vitro studies on the effects of multiple

gene knockouts in Francisella and Salmonella in BSL-3 and BSL-2 facilities.

Post-Doctoral Researcher Nov. 2003- Nov. 2006

Accomplishment: Published 2 papers, presented 7 posters / oral, Awarded 1

best posters / awards, 1 grant funded from NIH. Design and perform in vivo

and in vitro studies on the effects of multiple gene knockouts in

Francisella and Salmonella in BSL-3 and BSL-2 facilities.

University of Texas Health Science Center, San Antonio, Texas

Department of Microbiology

Post-Doctoral Fellow May 2002- Dec. 2003

Accomplishment: Perform and plan independent experiment to understand the

Bacillus subtilis sporulation induction under nutrient starvation.

Developed mariner transposon system for Bacillus species to make transposon

library and identify the virulence genes in Bacillus anthracis.

Center for Tuber Crop Research Institute, Bhubaneswar, India

Department of Microbiology

Research Associate Oct. 1996- Sept. 1997

Accomplishment: Identified fungi sweet potato rot during harvest season,

published one paper and presented an oral talk in a scientific meeting at

Trivendram, India.

Jawaharlal Nehru University, New Delhi, India

Center for Biotechnology

Junior Research Fellow May 1995- Aug. 1996

PI: Dr. Santos K Kar

Accomplishment: Identified dandruff causing bacterial and fungal species

isolated from human scalp, submitted project reports to the funding agency.

Honors and Awards

. Travel award for the Poster presentation, 3rd Center for Microbial

Inteface Biology retreat, Ohio State University, Columbus, Ohio, March

18th-19th, 2010.

. Senior Research Fellowship by Indian Council of Medical Research

(ICMR), May 1999 to 2002.

. Best Paper award by Hospital Infection Society, India (2001).

. Young Scientist Award by Molecular Epidemiology and Evolutionary

Genetics of Infectious Diseases, Vth International Conference (2000).

. Best Poster award by Indian Association of Medical Microbiologists,

Delhi Chapter (1997).

. Junior Research Fellow, Jawaharlal Nehru University, January 1995-

August 1996.

Project Management and Co-Investigator

Ongoing

. Rational design of high affinity peptide drug candidates, Funded by

NIH, Sept. 2010- Aug. 2013.

. Molecular epidemiology of key issues of food borne pathogens in

Eastern Africa, Funded by NIH Jun 2010- May 2015.

. Salmonella antimicrobial peptide resistance, Funded by NIH, Mar 2009-

Feb 2014.

. Great Lakes" Regional Center of Excellence in Biodefense and

EmergingInfectious Diseases Host and bacterial determinants in the

pathogenesis of pneumonic tularemia, Funded by NIH, Mar 2009-Feb 2014.

Completed

. A POC field test for the rapid determination of folate. Funded by HHS,

Sept. 2012-Mar. 2013.

. Pseudo-autotrophic selection of Francisella tularensis pentose-

phosphate auxotrophs. Funded by NIH, Sept 2007-Aug. 2010.

. Great Lakes" RCE in Biodefense and Emerging Infectious Diseases Lung

Innate Immune Responses To Francisella tularensis: A Central Role For

The Macrophage. Funded by NIH, Jan 2006-Jan 2009.

. Development of a bacteriophage lambda delivery system as a vaccine

candidate against intracellular bacterial pathogens. Funded by

Phagevax Inc.

. Regulation of Virulence by PmrA in Francisella spp. Funded by NIH, Feb

2006-Jan 2008.

Submitted

. Development of RNA-based vaccine/adjuvant to control bacterial

infection, Funding agency-NIH.

. Macrophage Targeted Delivery of Novel Anti-Francisella Drugs, Funding

agency-NIH.

. Polymeric microparticles for macrophage-targeted delivery of

tuberculosis drugs, Funding agency-NIH.

. A point-of-care device to measure the platelet function in trauma

patients. Funding agency-DoD.

. Develop a Sterility Assuring Biocompatible Layer Spray Deposition

approach for rapid disinfection of large area burn wounds, Funding

agency-DoD.

Organisms Familiar With

Francisella novicida, Francisella tularensis (LVS), Vibrio cholera,

Staphylococcus aures (MRSA), E. coli, Salmonella enterica serovar Typhi,

Salmonella enterica serovar Typhimurium, Salmonella enterica serovar

Paratyphi, Francisella tularensis (Schu S4), Streptococcus spp, Candida

albicans, Klebsiella spp. Lactobacillus spp., Listeria spp. Campylobacter

spp. Acinetobacter spp. Helicobacter spp. Candida spp. Histoplasma spp.

Cryptococcus spp.

Computer Skills

. Microsoft Office (Word, excel, power point, outlook)- 18 years

. Adobe Photoshop- 18 years

. Adobe Acrobat-18 years

. Adobe CS4 for web design- 3 years

. SPSS-10 years

. Prism- 10 years

. Endnote-15 years

. Vector NTI-12 years

. DNAman-13 years

. Geneous-13 years

Membership of Professional Society

American Society of Microbiology

Hospital Infection Society

References will be provided on request

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