Assistant Engineering

Helen Mingmei Li

**** ******** **

Sugar Land, TX 77479

Cell: 832-***-****, Email: *********@*****.***

Objective

A Laboratory Research Assistant position utilizing my excellent skills

in chemistry and Biology.

QUALIFICATIONS:

Many years of lab experience in chemistry/ Biology research both in

academic and industrial organizations.

Strong organizational and problem solving skills.

Capable of handling concurrent projects.

Able to work both independently and collaboratively.

PROFESSIONAL EXPERIENCE

Laboratory

? Analyzed the composition of raw materials

? Extracted and purified DNA/RNA

? Gene Cloning

? Performed synthesis, purification, distillation and crystallization

? Carried out solubility experiments

? Handled and storage of chemicals

Analytical

? Analyzed and interpreted experimental data qualitatively preformed

? Conducted statistical analysis of experimental data

Communication

? Wrote clear and concise laboratory reports

? Presented ideas and results to team members and supervisor

Organizational

? Designed and organized laboratory experiments

? Supervised the production

Work Chronology

2009-currently

Research Assistant(Clinical Laboratory)

Baylor College of Medicine

Extracting total RNA from Blood/Bone Marrow(Ribopure)

Extracting DNA from Blood/Tissue

BCR-ABL quantitative test( RT-qPCR) and data analysis

PML-RARA qualitative test and analysis

JAK2-V617F qualitative test and analysis

Illumina precapture/capture preparation of library

Solid precapture/capture preparation of library

454 Recombinants 3kb/8kb preparation of library

Solid Total RNA-Seq: whole Transcriptome/ Small RNA Library Preparation

DNA/RNA chip Bio analyzer

Pico/Ribogreen Bio analyzer

2008-2009

Lab Technician

Epoch Biolabs Inc, Houston, TX, USA

Gene synthesis

PCR, DNA sequencing reaction

DNA electrophoresis

DNA purification

Molecular Cloning

Miniprep Plasmid

Muteagenesis

2007-2008

Federal Medical Claims Assessor

Resolve Corporation, Ottawa, ON, Canada

EDUCATION

2005-2006

1-year professional work training of Biotechnology for adapting North

American Labs in Inno. Biotech. Inc. Toronto, ON, Canada.

DNA isolation electrophoresis

PCR and fragment purification

Gene cloning and vector

Sub-cloning and restriction endonulease

RNA and its characterization

RT-PCR, Nested PCR, LD-PCR and its application

5'-&3'-ready cDNA synthesis

5'-&3'-SMART RACE cDNA Amplification

Proteins expression in E. coli

Tissue protein isolation

Western blotting

Gene bank search and NCBI BLAST

2001-2003 Certificates of Medicine,

Toronto International Institute of TCM and Acupuncture, Toronto,

Ontario

Related courses:

Anatomy, Basic Theory of Traditional Chinese Medicine (TCM)

TCM Diagnostics, Acupuncture, TCM Gynecology

Herbalogy and Formulas

1986-1990 Bachelor of Science, Chemical Engineering

Qingdao University of Science & Technology, China,

Diploma thesis: Chemical modification of polyethylene

Related courses:

Inorganic chemistry, Analytical chemistry, Organic chemistry,

Physical chemistry, Polymer chemistry, Polymer physics,

General physics, Principles of chemical engineering, Electrotechnics,

Engineering mechanics, Advanced mathematics, Engineering,etc.

Contact this candidate