Project Assistant
Resume:
Marc Chalow Bostrom
**** ***** ***.-********, ** 08361
********@*******.***
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Bio-Tech/Microbiology Researcher
Education
MS in Biotechnology Thomas Jefferson University PA GPA: 3.59
2010
BS in Biology Lock Haven University PA
GPA: 3.0 2007
Profile of Qualifications
Key Studies:
Molecular Biology
Basic Molecular Techniques
Molecular Prep Techniques
Molecular Diagnostic Techniques
Microbial Genetics
Protein Purification & Characterization
Regulatory/Fiscal Issues in Lab Mgmt
Pathology
Cell & Tissue Culture
Research Design
Systems Biology
Immunology
Biochemistry
Human Genetics
Laboratory Proficiencies:
. Western Blot ~ BLAST databank ~ Spectrophotometric analysis ~ DNA
fingerprinting ~ PCR/RT-PCR ~ Cell counting ~ Histo staining/imaging
. Cell growth and tissue culture ~ Transformation ~Recombinant protein
expression-T7 system ~ Cell-Free Expression System (The Expressway
Mini) ~ DIG random-primed DNA labeling
. X-Gal Staining (beta-Galactosidase)
. Detecting genomic polymorphisms using Ready-To-Go RAPD Analysis Beads
~ ARMS-PCR assay ~ Mitochondrial DNA-based ID testing
. Expression of GFP using a Galactose inducible expression system
(yeast)
. pAcGHLT-C baculovirus transfer vector (insect cell transfection)
. Mammalian Transfection Systems using 293T cells (human kidney cell
derived cell line)/
Ca2PO4 mediated DNA Transfection
. Antigen-antibody reactions (precipitation, agglutination, and passive
agglutination)ANA screening
. Solution and media preparations ~ Gel preparations (SDS-PAGE and
agarose) ~ Plasmid preps
. Restriction endonuclease based analysis ~ Gel column/Affinity/Ion
Exchange filtration ~ Bradford protein assay
. Electrophoresis (Protein and nucleic acid) ~ Basic protein
purification
. Use of liquid handling devices, i.e., micropipettes ~ Data imaging and
analysis ~ Sterile technique
. Micro-CT imaging ~ Compression testing
. GAG assay ~ Picogreen assay ~ OHP assay
. Experience in handling mice
. Experience growing plants
> PRACTICAL RESEARCH BACKGROUND in Recombinant Protein Expression, E.coli,
genotype-phenotype mutation database project and in CNS regeneration
literature project; proficient in literature searches/reviews
> GENERAL LAB ASSISTANT/Thomas Jefferson University
Prepared solutions, autoclaved glassware/plastic ware and washed
glassware as well as assisted with laboratory sessions setup for high
school students
Marc Chalow Bostrom
3239 Dante Ave.-Vineland, NJ 08361
********@*******.***
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Research Experience
Thomas Jefferson University Literature Projects Overview
September 2009-April 2010
. Grape Seed Extract Inducing Cell Cycle Arrest and Apoptosis in Human
Colon Carcinoma Cells
. Gene Therapy for PKU Disorder
. Gene Therapy for Tyrosinemia type 1
. Down Syndrome
. Panitumumab and Prasugrel (treatment review)
. Histopathology Case 619
. Why can't I just take a Pill to Lose Weight? (group report)
Thomas Jefferson University Literature Research Project I & II
May-August 2010
. Successfully performed research on CNS regeneration and created and
delivered PowerPoint presentation to instructors and classmates
. Included comprehensive literature review; effectively gathered data on
current state and future prospects of CNS regeneration
Database Project
May-June 2010
. Effectively created genotype-phenotype mutation database for ABCR via MS
Excel, resulting in 309+ database entries; presented findings to Director
of Biotechnology for use in ongoing genetic research
. Conducted PubMed literature search and analyzed data to define
information relevant to mutations
Recombinant Protein Expression in E.coli
May-August 2010
. Facilitated generation and purification of plasmid DNA from wild-type and
mutant recombinant strains and verified plasmid insert via restriction
endonuclease digestion
. Research included transformation of DE3 E.coli cell line for use in
recombinant protein production and expression of recombinant proteins
using IPTG inducible T7 system as well as verification of correct
polypeptide using SDS-PAGE analysis followed by Coomassie and/or silver
staining
. Employed electronic photo documentation of SDS-PAGE
. Prepared frozen stabs for archiving of recombinant strains; stored in
-80 C freezer for future experimental use
Work Experience
University of Pennsylvania Temporary Research Assistant
February 2013-December 2013
. Handle overflow in the Histology portion of the Department of
Orthopaedic Surgery laboratories.
. Perform tasks such as: sectioning slides with a microtome; staining
slides; imaging slides with an Eclipse microscope; analyze slides with
the micro-CT machine; compression testing; OHP, GAG, and Picogreen
assays.
Excellent References Available Upon Request
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