Development Assistant

*** ********** ***

Gregory Bonello, PhD

Oceanside CA 92057

Permanent resident of the United States

*********@*****.*** Ph: 760-***-****

Page 1 of 3

MOLECULAR AND CELL BIOLOGIST – Industry contractor Scientist

Open to long term opportunity – Willing to relocate (USA, Europe, worldwide)

SUMMARY

● Industry experience (September 2013 – present) – Research and Development – Validation

● Genome Editing and Cell Engineering – Gene and Cell therapy

● Vaccine and assay development – Stem Cell biology

● Cell isolation (blood, stem cells), purification, culture and in vitro differentiation

● Extensive experience in Flow cytometry and Cell sorting

● Excellent communication skills (oral/poster presentations and illustrations)

● Laboratory management – Supervisor of students (high school – Master level)

● Safety management – Ionizing reagents and Biosafety level 3 (BSL-3) safety training (2010)

SKILLS

● Genome Editing and Cell Engineering – Zinc Finger Nuclease and CRISPR technologies

● Recombinant DNA techniques – Automation systems – Next Generation Sequencing

● Recombinant protein techniques – Production, purification and characterization – Mass Spectrometry

● Microscopy – Confocal Microscopy – Time-Lapse Confocal Microscopy - Immunofluorescence

● Viral capsid (VLP) production and purification – mitochondrial and lipid raft isolation - Microinjection

● Bioinformatics and data processing – Vector NTI, NEB cutter, sequence alignments, sequence blasts,

in silico determination of transcription factor binding sites, Cytoscape, FloJo

● Signal transduction – Gene regulation – Epigenetics - Oncology

● Immunology – Microbiology – Virology – Embryology – Physiology – Neurophysiology

● Mice handling, use, care and breeding

EDUCATION

● PhD Immunology – Universite de la Mediterranee, Marseille, France (May 2004)

● MS Molecular and Cell Biology – Universite J Fourier Grenoble, France (June 1997)

● BS Cell Biology and Physiology – Universite J Fourier Grenoble, France (June 1995)

WORK HISTORY

Sept 2013 – Present: Contractor Scientist at Life Technologies, Carlsbad, CA – Research and

Development, Genetic Circuits and Cell Engineering Group – Verification and Validation

Development of molecular tools for Genome Editing and Cell Engineering

● Development and optimization of CRISPR technology tools

● Verification and Validation of the GeneArt CRISPR Nuclease Vector Kits

Page 2 of 3

Aug 2010 – Jan 2013: Staff Scientist at the TBRI, San Antonio, TX

Development of new approaches to an effective HIV vaccine

● Development of molecular tools (retroviral backbones)

● Production of viral vectors (VSV-G pseudotyped retroviruses)

Generation of Rhesus macaque cells resistant to SIV infection as a model for the development of

a cure for HIV infection

● Zinc-Finger Nuclease technology - Development of molecular tools (adenoviral vectors)

● Stem cell isolation (blood and bone marrow), purification, culture and in vitro differentiation

● Flow cytometry (cell marker characterization – cell population/purity characterization)

Additional responsibilities

● Supervisor of a Master student and laboratory management

● Preparation of oral/poster presentations (symposia and local scientific events)

● Preparation of biohazard/hazardous material and recombinant DNA authorization requests

● Assistant of the Principal Investigator for the preparation of grant applications

● Assistant of the Principal Investigator for the preparation of IACUC authorization requests

● Active member of the organizing committee of the 30th Annual Symposium for

Non Human Primate Models for AIDS

Jul 2009 – Mar 2010: Research Fellow at the UC Irvine, CA

Determination of short RNA landscapes in Trypanosoma brucei

● Mitochondria purification, RNA purification and establishment of cDNA libraries by SMART

● High throughput (next generation) sequencing

Characterization of the RNA editing machinery in Trypanosoma brucei

● Establishment of stable T brucei transfectants

● Tandem affinity protein purification, mass spectrometry analysis, use of Cytoscape software

Nov 2004 – Apr 2009: Postdoctoral Fellow at the UT Health Science Center at San Antonio, TX

Study of the regulation of the transcription of Ccl2 (Mcp-1) and Ccr5 genes – Assay developments

● Cell isolation (blood), purification and culture – Flow cytometry (cell marker/population characterization)

● Development and optimization of molecular assays: in vivo footprinting by linker-medited PCR (LM-

PCR), chromatin immunoprecipitation (ChIP), chromatin accessibility assay by real-time PCR (ChART-

PCR) and chromosome conformation capture (3C)

Mar 2000 – May 2004: Doctoral Fellow at the Unite INSERM U119 at Marseille, France

Study of the dynamic recruitment of the adaptor protein LAT within the immunological synapse upon

T cell stimulation

● Flow cytometry (population characterization and sorting)

● T cell-Antigen presentation cell conjugate formation

● Lipid raft isolation on sucrose gradient

● Confocal microscopy and time-lapse confocal microscopy (video-imaging)

Page 3 of 3

Contact this candidate