Protein Preparation_Certified Engineer/biomedical scientist

Maoshih Liang

*** ******* **** **. #* Amherst NY 14228 716-***-****

*******.*****@*****.***

SUMMARY

. Certified multidisciplinary engineer with 5+ years of experience in

bioprocess development: cell culture, proteins expression/purification

and bioreactor operation.

. Experienced in process optimization, hands-on analytical techniques and

technical documentation for tech transfer/publications. Equipped with

cGMP/GLP essentials.

. Competent communicator with proven track record in leading

research/groups, collaborating cross-functionally, mentoring less-

experienced colleagues and presenting in international conferences.

EDUCATION AND CERTIFICATES

. Fundamentals of Engineer Certificate (FE/EIT) New York

(2010)

. Doctoral Candidate, Chemical and Biological Engineering

SUNY at Buffalo (UB) (2007 - present)

. Master of Science, Chemical Engineering

National Taiwan University (NTU) (2007)

. Bachelor of Science, Chemical Engineering

National Taiwan University (NTU) (2004)

PROFESSIONAL EXPERIENCE

Bioprocess Engineering

. Engineered proteins for controlled release in artificial tissues

(SUNY at Buffalo, UB)

> Stably (cell lines development) or transiently (transfection)

expressed recombinant proteins in E. coli, HEK 293T and CHO cells.

Designed series of purification process including refolding,

filtration and preparative chromatography in AKTA systems

(affinity, ion exchange, size-exclusion, hydrophobic interaction

etc.)

> Quantified and characterized proteins using spectrophotometry,

Bradford assay, ELISA, electrophoresis (SDS-PAGE and Western

blot), circular dichroism (CD), EMSA, enzymatic kinetics analysis

and luciferase promoter-activity assay.

> Evaluated the effects of engineered proteins on tissues by DNA

extraction, mRNA isolation (qPCR), Instron, histology,

immunohistochemistry, contractility assay, and ECM proteins

analysis.

. Analyzed normal-flow filtration (NFF) filtration process. Correlated

the resistance to microorganisms' surface properties. Equipped with

member separation knowledge (TFF, DF, RO, UF, MF, etc.). ((National

Taiwan University, NTU)

. Utilized microorganisms to remediate oil-polluted solution. Monitored

pollutants degradation using gas chromatography (GC) and HPLC.

Established a swift estimation assay (2days down to 5mins) and

validated this assay in a scaled-up fed-batch bioreactor (NTU)

. Developed a novel lentiviral array to study real-time gene regulation

during stem cells differentiation. Validated this assay using

conventional techniques and functional staining (Alcian Blue, Von

Kossa, Oil Red O). Applied this assay and DOE to screen reagents for

medium formulation (UB)

Technology Development and Evaluation

. Invented a multi-well bioreactor for research of mechano-transduction

in 3-D tissues: computer-aid design, hands-on machine shop

manufacture, and implementation. Authored technical document/SOPs for

technology transfer (provisional patent filed, honorable mention in

UB Publications)

. Enhanced DNA delivery efficiency into hard-to-transfect cells by 10-

fold through optimizing magnetofection protocol. (UB)

. Evaluated a new continuous centrifugal unit operation process for

liquid of extreme viscosity treatment. Demonstrated a 10-100 fold

efficiency improvement. (NTU)

. Designed a chemical plant: process design/simulation and economy

analysis. (NTU)

Management/Mentorship

. Participated simultaneously in multiple research projects. Worked

independently or with internal or external collaborators of

interdisciplinary backgrounds. (UB, NTU)

. Managed the supply inventory in the lab and placed orders when

necessary. (UB, NTU)

. Personally trained/supervised 5+ PhD, MS, undergraduate or high

school students to familiarize with related techniques and to develop

research projects. (UB, NTU)

Professional Involvement

. Serves as reviewers for FASEB Journal and Journal of Controlled

Release

. Holds membership of Biomedical Eng. Society (BMES) & American

Institute of Chem. Eng. (AIChE)

TECHNICAL SKILLS

. Data Analysis : Statistical Analysis, Numerical Methods

. Software Proficiency : UNICORN, Preview, UniSim, AutoCAD, Fortran,

Matlab, MS Office

LEADERSHIP EXPERIENCES

Sharpened leadership and communication skills by participating in various

societies

. Active member of Toastmasters Club (2013-present): an international club

helps members gain public speech and communication skills.

. Rifle Private/Second Lieutenant Reserve, ROC (Taiwan) Army (2004):

military training, leadership/team-spirit building, first-aids/EMT basics

. Vice President (2010-2011) and Senator (2009-2010) of graduate student

associations: the liaison between students and university. Attended

meetings to seek physical/financial aids for students

. Class Representative of Chemical Engineering Department, NTU (Sep. 2001-

Jun. 2004)

PUBLICATIONS

Patent

1. D. Swartz, S. T. Andreadis, M. S. Liang, M. Koobatian, "Multi-well

Pulsation Devices" (provisional patent application filed by UB

STOR(6712))

Scientific Journals

2. S. Son*, M. S. Liang*, P. Lei, and S. T. Andreadis "Using Magnetic Force

to Achieve High-Efficiency DNA Delivery into Mesenchymal Stem Cells:

Overexpressing Nanog to promote proliferation and myogenesis" (In

preparation, *contributed equally)

3. M. S. Liang*, R. Padmashali*, P. Mistriotis*, and S. T. Andreadis

''Establishment of Regulatory Fingerprint of Mesenchymal Stem Cell

Differentiation: Applying Real-time, Live Cell Arrays" Stem Cells (Under

Revision, *contributed equally)

4. M. S. Liang, M. Koobatian, P. Lei, D. Swartz, S. T. Andreadis,

"Differential and Synergistic Effects of Mechanical Stimulation and

Growth Factor Presentation on Vascular Wall Function" Biomaterials 34(30)

7281-7291 (2013)

5. C. W. Liu, M. S. Liang, Y. C. Chen, L. A. Sayavedra-Soto and H. S. Liu

"Biodegradation of n-alkanes at high concentration and correlation to the

accumulation of H+ ions in Rhodococcus erythropolis NTU-1" Biochemical

Engineering Journal 63(15) 124-128 (2012)

6. M. S. Liang and S. T. Andreadis "Engineering fibrin-binding TGF-?1 for

sustained signaling and contractile function of MSC based vascular

constructs" Biomaterials 32-33-868*-**** (2011)

7. C. Y. Chiang, Y. S. Chen, M. S. Liang, F. Y. Lin, C. Y. D. Tai and H. S.

Liu "Absorption of Ethanol into Water and Glycerol/Water Solution in a

Rotating Packed Bed" Journal of the Taiwan Institute of Chemical

Engineers 40, 418-423 (2009)

Conference Papers

8. M. Koobatian, M. S. Liang, D. Swartz, S. T Andreadis, "Comparing the

Effects of Mechanical Stimulation on Bone Marrow and Hair-Follicle

Mesenchymal Stem Cells: Vascular Tissue Engineering" AIChE 2013, San

Francisco, Nov. 2013 (Oral Presentation)

9. P. Mistriotis, M. S. Liang, S. T. Andreadis, "Ectopic Expression of

Nanog Up-Regulates Srf and Reverses the Loss of Myogenic Differentiation

Capacity of Human Mesenchymal Stem Cells Due to Senescence" AIChE 2013,

San Francisco, Nov. 2013 (Oral Presentation)

10. M. S. Liang, S. Son, S. Sinha, S. T. Andreadis, "Engineering Nanog

Protein for Effective Protein Transduction: A Possible Alternative to

Reverse the Effects of Organismal Aging On Mesenchymal Stem Cells" AIChE

2013, San Francisco, Nov. 2013 (Oral Presentation)

11. S. Son, M. S. Liang, P. Lei, S. T. Andreadis, "Nanog Transient

Overexpression With Optimized Magnetofection to Reverse the Effects of

Organismal Aging On Mesenchymal Stem Cells" AIChE 2013, San Francisco,

Nov. 2013 (Poster Presentation)

12. P. Mistriotis, M. S. Liang, S. T. Andreadis, "Nanog enhances the

proliferation and reverses the effect of senescence on myogenic

differentiation of human Mesenchymnal Stem Cells" BMES 2013, Seattle,

Sep. 2013 (Poster presenter)

13. M. S. Liang, M. Koobatian, D. Swartz, S. T. Andreadis, "Development of

Biomimetic Environments with Appropriate Chemical and Mechanical Cues for

Cells in Bioengineered Vascular Grafts" BMES 2013, Seattle, Sep. 2013

(Poster presenter)

14. M. Koobatian, M. S. Liang, D. Swartz, S. T. Andreadis, "Differential

response of mesenchymal stem cells from different anatomic locations to

long-term culture and mechanical stimulation" BMES 2013, Seattle, Sep.

2013 (Poster presenter)

15. S. Son, M. S. Liang, P. Lei, S. T. Andreadis, "Non-viral DNA delivery

approach for high-efficiency Nanog transient overexpression in

mesenchymal stem cells to reverse the effects of organismal aging" BMES

2013, Seattle, Sep. 2013 (Oral presenter)

16. M. S. Liang, M. Koobatian, D. Swartz, S. T. Andreadis, "Development of

Biomimetic Environments with Appropriate Chemical and Mechanical Cues for

Cells in Bioengineered Vascular Grafts" AIChE 2012, Pittsburgh, Oct. 2012

(Oral presenter)

17. M. S. Liang*, R. Padmashali*, P. Mistriotis*,and S. T. Andreadis "Live-

Cell Screens for Studying Regulatory Networks in Human Mesenchymal Stem

Cell Differentiation" AIChE 2012, Pittsburgh, Oct. 2012 (Oral presenter,

*contributed equally)

18. M. S. Liang*, R. Padmashali*, P. Mistriotis*, and S. T. Andreadis

''Using Live Cell Arrays to Develop Gene Regulation Fingerprint for

Mesenchymal Stem Cell Differentiation Research" BMES 2012, Atlanta, Oct.

2012 (Poster, *contributed equally)

19. P. Mistriotis, M. S. Liang, J. Han, S. T. Andreadis "Nanog Reverses the

Effect of senescence on Myogenic Differentiation of Human Mesenchymal

Stem Cells" BMES 2012, Atlanta, Oct. 2012 (Poster)

20. R. M. Padmashali*, M. S. Liang*, P. Mistriotis*, S. T. Andreadis,

"Developing Signature Gene Regulation Patterns for Monitoring Mesenchymal

Stem Cells Differentiation Using Live Cell Arrays" NYSTEM 2012, New York

City, May 2012 (Poster, *contributed equally)

21. H. Peng, M. S. Liang, S. Row, E. Schlaich, D. D. Swartz, S. T.

Andreadis, "Implantable Arterial Vascular Grafts Based on Hair Follicle

Derived Mesenchymal Stem Cells and Small Intestinal Submucosa" NYSTEM

2012, New York City, May 2012 (Poster)

22. M. S. Liang and S. T. Andreadis "Covalent Immobilization of

Transforming Growth Factor-?1 (TGF-?1) for Enhanced Vascular

Functionality in vitro, Perhaps through prolonged activation of TGF-? 1

Pathway" AIChE 2011, Minneapolis, Oct. 2011 (Oral presenter)

23. M. S. Liang and S. T. Andreadis "Genetically engineered TGF- ?1 that

Binds to Fibrin and Enhances the Function of Vascular Grafts with MSC

Derived Smooth Muscle Progenitor Cells" BMES 2011, Harford, Oct. 2011

(Oral presenter)

24. M. S. Liang and S. T. Andreadis "Covalent Conjugation of Transforming

Growth Factor-?1 to fibrin hydrogel for Tissue Engineering" AIChE 2010,

Salt Lake City, Nov. 2010 (Oral presenter)

25. M. S. Liang and S. T. Andreadis "Covalent Conjugation of Transforming

Growth Factor-?1 to fibrin hydrogel for Tissue Engineering" BMES 2010,

Austin, Oct. 2010 (Oral presenter)

26. H. S. Liu, Y. S. Chen, M. S. Liang, C. C. Lin, and W. T. Liu "Mass

Transfer Enhancement in a Rotating Packed Bed for Non-Newtonian Fluid"

Symposium of Transport Phenomena and Applications, Taipei, Nov. 2003

(Oral presenter)

Thesis

27. M. S. Liang "The Applications of Cell-Pellets Forming and Protons

Releasing in Microbial Bioremediation" Master Thesis, National Taiwan

University (2007)[pic]

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